Hydrotalea sandarakina

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Hydrotalea

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2813 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7RVH0|A0A2W7RVH0_9BACT PAS domain S-box-containing protein OS=Hydrotalea sandarakina OX=1004304 GN=LX80_01530 PE=4 SV=1
MM1 pKa = 7.54LVQEE5 pKa = 4.81YY6 pKa = 10.53KK7 pKa = 9.19RR8 pKa = 11.84TYY10 pKa = 10.6YY11 pKa = 10.19PILQLTDD18 pKa = 3.24TAKK21 pKa = 10.22QAIVLMEE28 pKa = 4.9DD29 pKa = 3.83YY30 pKa = 10.34FVEE33 pKa = 4.13HH34 pKa = 6.75WPVCTNGTFVGILNRR49 pKa = 11.84DD50 pKa = 3.27ALASVSEE57 pKa = 4.14EE58 pKa = 3.78ASLSEE63 pKa = 5.39LIDD66 pKa = 3.37EE67 pKa = 5.79FIPVAVLNTDD77 pKa = 4.81FIFTAVKK84 pKa = 10.05LAALNHH90 pKa = 5.65LTLVPVVTPEE100 pKa = 4.32NILDD104 pKa = 4.75GIVTANDD111 pKa = 3.66LLRR114 pKa = 11.84ATAHH118 pKa = 4.65YY119 pKa = 9.42TGIEE123 pKa = 4.19LPGGTIVLQMEE134 pKa = 4.18RR135 pKa = 11.84KK136 pKa = 8.34EE137 pKa = 4.05YY138 pKa = 11.0SFGEE142 pKa = 4.0INRR145 pKa = 11.84LVEE148 pKa = 4.14TNDD151 pKa = 3.74AFITQLNSSINSNNGLLDD169 pKa = 3.86VIIKK173 pKa = 10.03INKK176 pKa = 8.06TEE178 pKa = 3.84ISDD181 pKa = 3.86VVATFQRR188 pKa = 11.84YY189 pKa = 7.62EE190 pKa = 4.19YY191 pKa = 10.93NVLYY195 pKa = 10.8YY196 pKa = 10.79LGDD199 pKa = 3.05EE200 pKa = 4.51WYY202 pKa = 9.75TNEE205 pKa = 5.81LKK207 pKa = 10.91DD208 pKa = 4.58NYY210 pKa = 9.92NNLMNYY216 pKa = 10.36LNVV219 pKa = 3.46

Molecular weight: 24.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7S965|A0A2W7S965_9BACT Mercuric transport protein MerT OS=Hydrotalea sandarakina OX=1004304 GN=LX80_02811 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 9.59RR3 pKa = 11.84TFQPHH8 pKa = 4.67KK9 pKa = 9.7RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.4SVHH16 pKa = 5.0GFRR19 pKa = 11.84KK20 pKa = 10.11RR21 pKa = 11.84MQTANGRR28 pKa = 11.84KK29 pKa = 8.63VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.28GRR39 pKa = 11.84KK40 pKa = 8.75KK41 pKa = 9.98LTVSSEE47 pKa = 4.1KK48 pKa = 10.7GLKK51 pKa = 9.34

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2813

0

2813

986317

39

2437

350.6

39.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.646 ± 0.046

0.899 ± 0.015

4.527 ± 0.034

5.158 ± 0.051

4.979 ± 0.035

6.415 ± 0.039

2.147 ± 0.024

7.899 ± 0.041

6.821 ± 0.038

9.534 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.382 ± 0.02

6.479 ± 0.048

4.004 ± 0.023

4.637 ± 0.031

3.25 ± 0.026

5.692 ± 0.039

5.854 ± 0.033

6.189 ± 0.033

1.269 ± 0.021

4.219 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski