Massilia sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Massilia; unclassified Massilia

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1435 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519PFU2|A0A519PFU2_9BURK Histidine kinase (Fragment) OS=Massilia sp. OX=1882437 GN=EOO64_03020 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 4.79EE3 pKa = 4.36EE4 pKa = 4.34LVANIVSFEE13 pKa = 4.08EE14 pKa = 4.39DD15 pKa = 3.0DD16 pKa = 4.04VMVAFAFCAHH26 pKa = 7.38EE27 pKa = 4.68GQSSDD32 pKa = 3.81YY33 pKa = 11.7LMLQYY38 pKa = 10.44PLQADD43 pKa = 3.61QQDD46 pKa = 3.41RR47 pKa = 11.84RR48 pKa = 11.84LRR50 pKa = 11.84WAPAWWDD57 pKa = 3.58WQPHH61 pKa = 6.22DD62 pKa = 4.51PHH64 pKa = 8.05ASSPGCDD71 pKa = 3.57AGRR74 pKa = 11.84CCQSIQEE81 pKa = 4.36PVPFGLSEE89 pKa = 3.89QDD91 pKa = 3.46QITT94 pKa = 3.37

Molecular weight: 10.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519PD14|A0A519PD14_9BURK AAA family ATPase (Fragment) OS=Massilia sp. OX=1882437 GN=EOO64_05035 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1435

0

1435

372938

25

1060

259.9

28.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.479 ± 0.088

0.833 ± 0.02

5.383 ± 0.057

5.515 ± 0.066

3.912 ± 0.049

8.205 ± 0.067

2.104 ± 0.035

5.013 ± 0.054

4.264 ± 0.063

10.104 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.035

3.242 ± 0.049

4.765 ± 0.042

3.761 ± 0.043

6.202 ± 0.067

5.534 ± 0.054

5.416 ± 0.047

7.584 ± 0.055

1.31 ± 0.03

2.656 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski