Amino acid dipepetide frequency for Massilia sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.171AlaAla: 15.171 ± 0.311
1.14AlaCys: 1.14 ± 0.066
6.025AlaAsp: 6.025 ± 0.143
6.347AlaGlu: 6.347 ± 0.14
4.025AlaPhe: 4.025 ± 0.098
9.484AlaGly: 9.484 ± 0.154
2.322AlaHis: 2.322 ± 0.083
5.524AlaIle: 5.524 ± 0.141
4.684AlaLys: 4.684 ± 0.127
12.305AlaLeu: 12.305 ± 0.196
3.346AlaMet: 3.346 ± 0.1
3.475AlaAsn: 3.475 ± 0.105
5.18AlaPro: 5.18 ± 0.145
4.631AlaGln: 4.631 ± 0.13
7.068AlaArg: 7.068 ± 0.129
6.261AlaSer: 6.261 ± 0.142
5.677AlaThr: 5.677 ± 0.121
7.983AlaVal: 7.983 ± 0.145
1.477AlaTrp: 1.477 ± 0.064
2.66AlaTyr: 2.66 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
1.056CysAla: 1.056 ± 0.058
0.11CysCys: 0.11 ± 0.017
0.464CysAsp: 0.464 ± 0.037
0.442CysGlu: 0.442 ± 0.034
0.306CysPhe: 0.306 ± 0.03
0.821CysGly: 0.821 ± 0.05
0.26CysHis: 0.26 ± 0.028
0.383CysIle: 0.383 ± 0.028
0.271CysLys: 0.271 ± 0.024
0.684CysLeu: 0.684 ± 0.048
0.236CysMet: 0.236 ± 0.027
0.233CysAsn: 0.233 ± 0.025
0.437CysPro: 0.437 ± 0.034
0.228CysGln: 0.228 ± 0.023
0.445CysArg: 0.445 ± 0.035
0.499CysSer: 0.499 ± 0.039
0.472CysThr: 0.472 ± 0.037
0.635CysVal: 0.635 ± 0.047
0.091CysTrp: 0.091 ± 0.015
0.255CysTyr: 0.255 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
6.264AspAla: 6.264 ± 0.129
0.405AspCys: 0.405 ± 0.036
2.633AspAsp: 2.633 ± 0.104
3.223AspGlu: 3.223 ± 0.091
2.378AspPhe: 2.378 ± 0.08
4.566AspGly: 4.566 ± 0.116
1.083AspHis: 1.083 ± 0.053
3.006AspIle: 3.006 ± 0.088
2.502AspLys: 2.502 ± 0.096
5.368AspLeu: 5.368 ± 0.114
1.397AspMet: 1.397 ± 0.063
1.528AspAsn: 1.528 ± 0.06
2.805AspPro: 2.805 ± 0.106
1.764AspGln: 1.764 ± 0.075
3.118AspArg: 3.118 ± 0.096
2.459AspSer: 2.459 ± 0.087
2.655AspThr: 2.655 ± 0.077
4.164AspVal: 4.164 ± 0.107
0.901AspTrp: 0.901 ± 0.052
1.609AspTyr: 1.609 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
6.248GluAla: 6.248 ± 0.131
0.4GluCys: 0.4 ± 0.033
2.448GluAsp: 2.448 ± 0.077
3.124GluGlu: 3.124 ± 0.08
1.976GluPhe: 1.976 ± 0.083
4.062GluGly: 4.062 ± 0.112
1.284GluHis: 1.284 ± 0.064
3.027GluIle: 3.027 ± 0.1
2.51GluLys: 2.51 ± 0.077
5.899GluLeu: 5.899 ± 0.139
1.603GluMet: 1.603 ± 0.061
1.847GluAsn: 1.847 ± 0.083
2.169GluPro: 2.169 ± 0.094
2.665GluGln: 2.665 ± 0.096
4.239GluArg: 4.239 ± 0.114
2.448GluSer: 2.448 ± 0.084
2.805GluThr: 2.805 ± 0.087
4.17GluVal: 4.17 ± 0.118
0.684GluTrp: 0.684 ± 0.047
1.542GluTyr: 1.542 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
4.242PheAla: 4.242 ± 0.097
0.383PheCys: 0.383 ± 0.035
2.649PheAsp: 2.649 ± 0.09
2.134PheGlu: 2.134 ± 0.063
1.59PhePhe: 1.59 ± 0.079
3.706PheGly: 3.706 ± 0.136
0.783PheHis: 0.783 ± 0.046
1.915PheIle: 1.915 ± 0.07
1.603PheLys: 1.603 ± 0.055
3.373PheLeu: 3.373 ± 0.113
0.955PheMet: 0.955 ± 0.056
1.373PheAsn: 1.373 ± 0.068
1.807PhePro: 1.807 ± 0.068
1.185PheGln: 1.185 ± 0.055
2.126PheArg: 2.126 ± 0.068
2.44PheSer: 2.44 ± 0.08
2.37PheThr: 2.37 ± 0.081
2.866PheVal: 2.866 ± 0.093
0.507PheTrp: 0.507 ± 0.041
1.107PheTyr: 1.107 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
8.607GlyAla: 8.607 ± 0.181
0.794GlyCys: 0.794 ± 0.048
4.041GlyAsp: 4.041 ± 0.111
4.416GlyGlu: 4.416 ± 0.122
3.518GlyPhe: 3.518 ± 0.113
6.733GlyGly: 6.733 ± 0.157
1.815GlyHis: 1.815 ± 0.078
4.457GlyIle: 4.457 ± 0.116
4.175GlyLys: 4.175 ± 0.12
8.224GlyLeu: 8.224 ± 0.14
2.655GlyMet: 2.655 ± 0.092
2.609GlyAsn: 2.609 ± 0.092
2.797GlyPro: 2.797 ± 0.09
3.137GlyGln: 3.137 ± 0.099
4.915GlyArg: 4.915 ± 0.107
4.639GlySer: 4.639 ± 0.114
4.422GlyThr: 4.422 ± 0.122
6.315GlyVal: 6.315 ± 0.141
1.274GlyTrp: 1.274 ± 0.061
2.512GlyTyr: 2.512 ± 0.085
0.0GlyXaa: 0.0 ± 0.0
His
2.309HisAla: 2.309 ± 0.089
0.26HisCys: 0.26 ± 0.025
1.075HisAsp: 1.075 ± 0.052
1.121HisGlu: 1.121 ± 0.055
0.925HisPhe: 0.925 ± 0.052
2.126HisGly: 2.126 ± 0.099
0.668HisHis: 0.668 ± 0.056
0.963HisIle: 0.963 ± 0.053
0.711HisLys: 0.711 ± 0.045
1.952HisLeu: 1.952 ± 0.081
0.491HisMet: 0.491 ± 0.037
0.544HisAsn: 0.544 ± 0.047
1.309HisPro: 1.309 ± 0.07
0.745HisGln: 0.745 ± 0.046
1.325HisArg: 1.325 ± 0.069
1.046HisSer: 1.046 ± 0.065
0.944HisThr: 0.944 ± 0.056
1.453HisVal: 1.453 ± 0.065
0.322HisTrp: 0.322 ± 0.032
0.756HisTyr: 0.756 ± 0.042
0.0HisXaa: 0.0 ± 0.0
Ile
6.312IleAla: 6.312 ± 0.143
0.453IleCys: 0.453 ± 0.034
3.454IleAsp: 3.454 ± 0.096
3.258IleGlu: 3.258 ± 0.099
1.77IlePhe: 1.77 ± 0.072
4.583IleGly: 4.583 ± 0.128
0.853IleHis: 0.853 ± 0.046
2.175IleIle: 2.175 ± 0.093
2.207IleLys: 2.207 ± 0.079
4.081IleLeu: 4.081 ± 0.111
1.091IleMet: 1.091 ± 0.045
1.906IleAsn: 1.906 ± 0.078
2.212IlePro: 2.212 ± 0.085
1.499IleGln: 1.499 ± 0.064
2.826IleArg: 2.826 ± 0.091
2.756IleSer: 2.756 ± 0.099
2.99IleThr: 2.99 ± 0.092
3.899IleVal: 3.899 ± 0.097
0.496IleTrp: 0.496 ± 0.038
1.309IleTyr: 1.309 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
4.808LysAla: 4.808 ± 0.114
0.22LysCys: 0.22 ± 0.024
2.287LysAsp: 2.287 ± 0.083
2.234LysGlu: 2.234 ± 0.089
1.365LysPhe: 1.365 ± 0.058
3.049LysGly: 3.049 ± 0.096
0.818LysHis: 0.818 ± 0.052
2.177LysIle: 2.177 ± 0.092
2.049LysLys: 2.049 ± 0.084
4.54LysLeu: 4.54 ± 0.117
1.185LysMet: 1.185 ± 0.062
1.636LysAsn: 1.636 ± 0.072
2.362LysPro: 2.362 ± 0.079
1.622LysGln: 1.622 ± 0.061
2.722LysArg: 2.722 ± 0.093
2.349LysSer: 2.349 ± 0.094
2.531LysThr: 2.531 ± 0.08
3.279LysVal: 3.279 ± 0.11
0.448LysTrp: 0.448 ± 0.037
0.96LysTyr: 0.96 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
12.302LeuAla: 12.302 ± 0.203
0.917LeuCys: 0.917 ± 0.051
5.73LeuAsp: 5.73 ± 0.137
5.1LeuGlu: 5.1 ± 0.134
3.907LeuPhe: 3.907 ± 0.111
8.017LeuGly: 8.017 ± 0.142
1.963LeuHis: 1.963 ± 0.075
4.722LeuIle: 4.722 ± 0.102
4.207LeuLys: 4.207 ± 0.101
10.382LeuLeu: 10.382 ± 0.221
2.671LeuMet: 2.671 ± 0.087
3.22LeuAsn: 3.22 ± 0.087
5.081LeuPro: 5.081 ± 0.123
3.762LeuGln: 3.762 ± 0.103
6.269LeuArg: 6.269 ± 0.148
6.108LeuSer: 6.108 ± 0.145
5.534LeuThr: 5.534 ± 0.134
7.663LeuVal: 7.663 ± 0.175
1.099LeuTrp: 1.099 ± 0.058
2.381LeuTyr: 2.381 ± 0.091
0.0LeuXaa: 0.0 ± 0.0
Met
3.081MetAla: 3.081 ± 0.104
0.212MetCys: 0.212 ± 0.023
1.298MetAsp: 1.298 ± 0.057
1.266MetGlu: 1.266 ± 0.07
0.957MetPhe: 0.957 ± 0.051
1.928MetGly: 1.928 ± 0.087
0.566MetHis: 0.566 ± 0.038
1.215MetIle: 1.215 ± 0.062
1.475MetLys: 1.475 ± 0.062
2.807MetLeu: 2.807 ± 0.099
0.694MetMet: 0.694 ± 0.045
0.968MetAsn: 0.968 ± 0.053
1.44MetPro: 1.44 ± 0.064
1.132MetGln: 1.132 ± 0.055
1.872MetArg: 1.872 ± 0.073
1.839MetSer: 1.839 ± 0.075
1.66MetThr: 1.66 ± 0.066
1.949MetVal: 1.949 ± 0.074
0.249MetTrp: 0.249 ± 0.025
0.56MetTyr: 0.56 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.652AsnAla: 3.652 ± 0.103
0.311AsnCys: 0.311 ± 0.028
1.641AsnAsp: 1.641 ± 0.066
1.673AsnGlu: 1.673 ± 0.06
1.196AsnPhe: 1.196 ± 0.069
2.84AsnGly: 2.84 ± 0.092
0.644AsnHis: 0.644 ± 0.038
1.593AsnIle: 1.593 ± 0.069
1.413AsnLys: 1.413 ± 0.069
3.215AsnLeu: 3.215 ± 0.102
0.762AsnMet: 0.762 ± 0.048
1.126AsnAsn: 1.126 ± 0.06
2.116AsnPro: 2.116 ± 0.08
1.024AsnGln: 1.024 ± 0.058
1.874AsnArg: 1.874 ± 0.071
1.566AsnSer: 1.566 ± 0.071
1.869AsnThr: 1.869 ± 0.078
2.48AsnVal: 2.48 ± 0.091
0.488AsnTrp: 0.488 ± 0.034
0.941AsnTyr: 0.941 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
5.843ProAla: 5.843 ± 0.152
0.265ProCys: 0.265 ± 0.028
2.944ProAsp: 2.944 ± 0.093
3.365ProGlu: 3.365 ± 0.082
1.893ProPhe: 1.893 ± 0.071
4.21ProGly: 4.21 ± 0.102
1.067ProHis: 1.067 ± 0.055
1.976ProIle: 1.976 ± 0.075
1.92ProLys: 1.92 ± 0.075
4.612ProLeu: 4.612 ± 0.122
1.115ProMet: 1.115 ± 0.06
1.494ProAsn: 1.494 ± 0.067
1.941ProPro: 1.941 ± 0.089
1.687ProGln: 1.687 ± 0.076
2.384ProArg: 2.384 ± 0.085
2.553ProSer: 2.553 ± 0.085
2.502ProThr: 2.502 ± 0.088
4.027ProVal: 4.027 ± 0.122
0.638ProTrp: 0.638 ± 0.046
1.217ProTyr: 1.217 ± 0.062
0.0ProXaa: 0.0 ± 0.0
Gln
4.432GlnAla: 4.432 ± 0.112
0.263GlnCys: 0.263 ± 0.024
1.724GlnAsp: 1.724 ± 0.068
1.896GlnGlu: 1.896 ± 0.087
1.448GlnPhe: 1.448 ± 0.067
2.832GlnGly: 2.832 ± 0.082
0.888GlnHis: 0.888 ± 0.046
1.976GlnIle: 1.976 ± 0.072
1.397GlnLys: 1.397 ± 0.063
3.842GlnLeu: 3.842 ± 0.11
1.089GlnMet: 1.089 ± 0.058
1.105GlnAsn: 1.105 ± 0.06
1.85GlnPro: 1.85 ± 0.068
1.856GlnGln: 1.856 ± 0.082
2.687GlnArg: 2.687 ± 0.079
1.92GlnSer: 1.92 ± 0.089
1.821GlnThr: 1.821 ± 0.067
3.068GlnVal: 3.068 ± 0.079
0.509GlnTrp: 0.509 ± 0.038
1.014GlnTyr: 1.014 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
6.17ArgAla: 6.17 ± 0.13
0.416ArgCys: 0.416 ± 0.037
3.489ArgAsp: 3.489 ± 0.088
3.7ArgGlu: 3.7 ± 0.106
2.81ArgPhe: 2.81 ± 0.09
4.36ArgGly: 4.36 ± 0.113
1.577ArgHis: 1.577 ± 0.064
3.607ArgIle: 3.607 ± 0.101
2.601ArgLys: 2.601 ± 0.082
6.315ArgLeu: 6.315 ± 0.147
1.965ArgMet: 1.965 ± 0.074
2.062ArgAsn: 2.062 ± 0.077
2.834ArgPro: 2.834 ± 0.096
2.534ArgGln: 2.534 ± 0.073
4.105ArgArg: 4.105 ± 0.138
3.325ArgSer: 3.325 ± 0.096
3.186ArgThr: 3.186 ± 0.101
4.395ArgVal: 4.395 ± 0.122
0.863ArgTrp: 0.863 ± 0.044
1.711ArgTyr: 1.711 ± 0.073
0.0ArgXaa: 0.0 ± 0.0
Ser
5.947SerAla: 5.947 ± 0.129
0.357SerCys: 0.357 ± 0.033
2.746SerAsp: 2.746 ± 0.094
2.864SerGlu: 2.864 ± 0.094
2.408SerPhe: 2.408 ± 0.08
5.13SerGly: 5.13 ± 0.132
1.166SerHis: 1.166 ± 0.058
2.845SerIle: 2.845 ± 0.093
2.105SerLys: 2.105 ± 0.075
5.719SerLeu: 5.719 ± 0.126
1.558SerMet: 1.558 ± 0.065
1.668SerAsn: 1.668 ± 0.072
2.609SerPro: 2.609 ± 0.085
1.799SerGln: 1.799 ± 0.075
3.368SerArg: 3.368 ± 0.105
3.427SerSer: 3.427 ± 0.116
3.186SerThr: 3.186 ± 0.106
4.103SerVal: 4.103 ± 0.113
0.815SerTrp: 0.815 ± 0.044
1.526SerTyr: 1.526 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
5.8ThrAla: 5.8 ± 0.135
0.4ThrCys: 0.4 ± 0.032
2.722ThrAsp: 2.722 ± 0.097
2.48ThrGlu: 2.48 ± 0.102
2.051ThrPhe: 2.051 ± 0.074
4.891ThrGly: 4.891 ± 0.13
1.102ThrHis: 1.102 ± 0.049
2.821ThrIle: 2.821 ± 0.104
1.96ThrLys: 1.96 ± 0.089
5.864ThrLeu: 5.864 ± 0.124
1.322ThrMet: 1.322 ± 0.059
1.719ThrAsn: 1.719 ± 0.079
3.263ThrPro: 3.263 ± 0.1
1.88ThrGln: 1.88 ± 0.078
3.295ThrArg: 3.295 ± 0.096
2.899ThrSer: 2.899 ± 0.089
3.084ThrThr: 3.084 ± 0.099
4.497ThrVal: 4.497 ± 0.117
0.711ThrTrp: 0.711 ± 0.051
1.4ThrTyr: 1.4 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
8.583ValAla: 8.583 ± 0.151
0.633ValCys: 0.633 ± 0.041
4.288ValAsp: 4.288 ± 0.114
4.491ValGlu: 4.491 ± 0.105
2.963ValPhe: 2.963 ± 0.093
5.685ValGly: 5.685 ± 0.124
1.351ValHis: 1.351 ± 0.063
3.896ValIle: 3.896 ± 0.103
3.231ValLys: 3.231 ± 0.106
7.741ValLeu: 7.741 ± 0.165
2.019ValMet: 2.019 ± 0.078
2.588ValAsn: 2.588 ± 0.09
3.593ValPro: 3.593 ± 0.104
2.756ValGln: 2.756 ± 0.084
4.564ValArg: 4.564 ± 0.114
4.389ValSer: 4.389 ± 0.098
4.132ValThr: 4.132 ± 0.118
6.194ValVal: 6.194 ± 0.132
0.861ValTrp: 0.861 ± 0.051
1.88ValTyr: 1.88 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
1.132TrpAla: 1.132 ± 0.059
0.153TrpCys: 0.153 ± 0.021
0.708TrpAsp: 0.708 ± 0.041
0.56TrpGlu: 0.56 ± 0.043
0.526TrpPhe: 0.526 ± 0.035
0.866TrpGly: 0.866 ± 0.05
0.316TrpHis: 0.316 ± 0.032
0.657TrpIle: 0.657 ± 0.043
0.609TrpLys: 0.609 ± 0.046
1.571TrpLeu: 1.571 ± 0.077
0.391TrpMet: 0.391 ± 0.035
0.485TrpAsn: 0.485 ± 0.035
0.544TrpPro: 0.544 ± 0.038
0.627TrpGln: 0.627 ± 0.048
1.048TrpArg: 1.048 ± 0.056
0.724TrpSer: 0.724 ± 0.049
0.772TrpThr: 0.772 ± 0.048
0.845TrpVal: 0.845 ± 0.056
0.223TrpTrp: 0.223 ± 0.028
0.341TrpTyr: 0.341 ± 0.031
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.826TyrAla: 2.826 ± 0.084
0.239TyrCys: 0.239 ± 0.026
1.464TyrAsp: 1.464 ± 0.065
1.456TyrGlu: 1.456 ± 0.064
1.105TyrPhe: 1.105 ± 0.053
2.167TyrGly: 2.167 ± 0.08
0.518TyrHis: 0.518 ± 0.038
1.196TyrIle: 1.196 ± 0.063
1.011TyrLys: 1.011 ± 0.05
2.542TyrLeu: 2.542 ± 0.091
0.622TyrMet: 0.622 ± 0.045
0.837TyrAsn: 0.837 ± 0.053
1.303TyrPro: 1.303 ± 0.072
1.081TyrGln: 1.081 ± 0.056
1.772TyrArg: 1.772 ± 0.074
1.697TyrSer: 1.697 ± 0.065
1.55TyrThr: 1.55 ± 0.078
1.853TyrVal: 1.853 ± 0.07
0.442TyrTrp: 0.442 ± 0.041
0.877TyrTyr: 0.877 ± 0.058
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1435 proteins (372938 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski