Cellulomonas composti 
Average proteome isoelectric point is 5.99 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3111 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A511JE20|A0A511JE20_9CELL Ribosomal RNA small subunit methyltransferase E OS=Cellulomonas composti OX=266130 GN=CCO02nite_28440 PE=3 SV=1 
MM1 pKa = 7.55  IGGAPGRR8 pKa = 11.84  WGGALLLVVLAVLTSACGGHH28 pKa = 6.74  AAATEE33 pKa = 4.13  SSNAEE38 pKa = 3.76  SVAFSGEE45 pKa = 4.06  VPEE48 pKa = 5.42  FEE50 pKa = 4.37  GPYY53 pKa = 9.85  AAEE56 pKa = 3.81  FADD59 pKa = 5.12  FYY61 pKa = 11.26  VHH63 pKa = 5.92  STSEE67 pKa = 3.82  FVRR70 pKa = 11.84  AVLADD75 pKa = 3.73  GEE77 pKa = 4.5  ITDD80 pKa = 3.85  AEE82 pKa = 4.35  YY83 pKa = 11.44  AEE85 pKa = 4.47  MVTRR89 pKa = 11.84  LSEE92 pKa = 4.22  CLDD95 pKa = 3.87  GYY97 pKa = 10.75  GVEE100 pKa = 4.59  LQSIAPGAGMSTTNAPNGADD120 pKa = 2.89  THH122 pKa = 6.51  EE123 pKa = 4.97  IINTCSVVAGEE134 pKa = 4.19  NSIGALRR141 pKa = 11.84  DD142 pKa = 3.94  FMADD146 pKa = 3.25  NPDD149 pKa = 3.73  NADD152 pKa = 3.58  PTPLIVQCLVDD163 pKa = 4.09  SGTVPSDD170 pKa = 3.38  YY171 pKa = 10.94  GVEE174 pKa = 4.57  DD175 pKa = 5.03  FDD177 pKa = 4.69  TDD179 pKa = 3.7  SQSRR183 pKa = 11.84  FTDD186 pKa = 3.79  LDD188 pKa = 3.32  QLDD191 pKa = 4.69  PGLSAAFTSCTMDD204 pKa = 3.11  VSRR207 pKa = 11.84  SMTADD212 pKa = 3.26  ASRR215 pKa = 4.06   
 Molecular weight: 22.31 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.732 
IPC2_protein 3.706 
IPC_protein 3.706 
Toseland    3.49 
ProMoST     3.859 
Dawson      3.694 
Bjellqvist  3.846 
Wikipedia   3.617 
Rodwell     3.528 
Grimsley    3.401 
Solomon     3.681 
Lehninger   3.63 
Nozaki      3.808 
DTASelect   4.024 
Thurlkill   3.541 
EMBOSS      3.63 
Sillero     3.821 
Patrickios  0.731 
IPC_peptide 3.681 
IPC2_peptide  3.795 
IPC2.peptide.svr19  3.741 
 Protein with the highest isoelectric point: 
>tr|A0A511JDR3|A0A511JDR3_9CELL Uncharacterized protein OS=Cellulomonas composti OX=266130 GN=CCO02nite_27700 PE=4 SV=1 
MM1 pKa = 7.69  SKK3 pKa = 9.0  RR4 pKa = 11.84  TFQPNNRR11 pKa = 11.84  RR12 pKa = 11.84  RR13 pKa = 11.84  AKK15 pKa = 8.7  THH17 pKa = 5.15  GFRR20 pKa = 11.84  LRR22 pKa = 11.84  MRR24 pKa = 11.84  TRR26 pKa = 11.84  AGRR29 pKa = 11.84  SILAARR35 pKa = 11.84  RR36 pKa = 11.84  RR37 pKa = 11.84  KK38 pKa = 9.61  GRR40 pKa = 11.84  AEE42 pKa = 3.84  LSAA45 pKa = 4.93   
 Molecular weight: 5.32 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.453 
IPC2_protein 10.979 
IPC_protein 12.574 
Toseland    12.749 
ProMoST     13.247 
Dawson      12.749 
Bjellqvist  12.735 
Wikipedia   13.217 
Rodwell     12.34 
Grimsley    12.778 
Solomon     13.247 
Lehninger   13.144 
Nozaki      12.749 
DTASelect   12.735 
Thurlkill   12.749 
EMBOSS      13.247 
Sillero     12.749 
Patrickios  12.076 
IPC_peptide 13.247 
IPC2_peptide  12.237 
IPC2.peptide.svr19  9.123 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3111 
 
        
        0
 
        
        3111 
         
        1071251
 
        39
 
        3147
 
        344.3
 
        36.5
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        14.323 ± 0.065
0.584 ± 0.011
 
        6.665 ± 0.03
5.226 ± 0.041
 
        2.509 ± 0.022
9.234 ± 0.04
 
        2.061 ± 0.022
2.983 ± 0.031
       
        1.489 ± 0.03
10.153 ± 0.057
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        1.496 ± 0.017
1.524 ± 0.024
 
        5.977 ± 0.039
2.665 ± 0.024
 
        7.72 ± 0.056
5.119 ± 0.034
 
        6.59 ± 0.064
10.282 ± 0.046
       
        1.564 ± 0.019
1.835 ± 0.021
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here