Cellulomonas composti
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3111 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511JE20|A0A511JE20_9CELL Ribosomal RNA small subunit methyltransferase E OS=Cellulomonas composti OX=266130 GN=CCO02nite_28440 PE=3 SV=1
MM1 pKa = 7.55 IGGAPGRR8 pKa = 11.84 WGGALLLVVLAVLTSACGGHH28 pKa = 6.74 AAATEE33 pKa = 4.13 SSNAEE38 pKa = 3.76 SVAFSGEE45 pKa = 4.06 VPEE48 pKa = 5.42 FEE50 pKa = 4.37 GPYY53 pKa = 9.85 AAEE56 pKa = 3.81 FADD59 pKa = 5.12 FYY61 pKa = 11.26 VHH63 pKa = 5.92 STSEE67 pKa = 3.82 FVRR70 pKa = 11.84 AVLADD75 pKa = 3.73 GEE77 pKa = 4.5 ITDD80 pKa = 3.85 AEE82 pKa = 4.35 YY83 pKa = 11.44 AEE85 pKa = 4.47 MVTRR89 pKa = 11.84 LSEE92 pKa = 4.22 CLDD95 pKa = 3.87 GYY97 pKa = 10.75 GVEE100 pKa = 4.59 LQSIAPGAGMSTTNAPNGADD120 pKa = 2.89 THH122 pKa = 6.51 EE123 pKa = 4.97 IINTCSVVAGEE134 pKa = 4.19 NSIGALRR141 pKa = 11.84 DD142 pKa = 3.94 FMADD146 pKa = 3.25 NPDD149 pKa = 3.73 NADD152 pKa = 3.58 PTPLIVQCLVDD163 pKa = 4.09 SGTVPSDD170 pKa = 3.38 YY171 pKa = 10.94 GVEE174 pKa = 4.57 DD175 pKa = 5.03 FDD177 pKa = 4.69 TDD179 pKa = 3.7 SQSRR183 pKa = 11.84 FTDD186 pKa = 3.79 LDD188 pKa = 3.32 QLDD191 pKa = 4.69 PGLSAAFTSCTMDD204 pKa = 3.11 VSRR207 pKa = 11.84 SMTADD212 pKa = 3.26 ASRR215 pKa = 4.06
Molecular weight: 22.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.706
IPC_protein 3.706
Toseland 3.49
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.541
EMBOSS 3.63
Sillero 3.821
Patrickios 0.731
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.741
Protein with the highest isoelectric point:
>tr|A0A511JDR3|A0A511JDR3_9CELL Uncharacterized protein OS=Cellulomonas composti OX=266130 GN=CCO02nite_27700 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.61 GRR40 pKa = 11.84 AEE42 pKa = 3.84 LSAA45 pKa = 4.93
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3111
0
3111
1071251
39
3147
344.3
36.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.323 ± 0.065
0.584 ± 0.011
6.665 ± 0.03
5.226 ± 0.041
2.509 ± 0.022
9.234 ± 0.04
2.061 ± 0.022
2.983 ± 0.031
1.489 ± 0.03
10.153 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.496 ± 0.017
1.524 ± 0.024
5.977 ± 0.039
2.665 ± 0.024
7.72 ± 0.056
5.119 ± 0.034
6.59 ± 0.064
10.282 ± 0.046
1.564 ± 0.019
1.835 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here