Yersinia phage JC221

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8PGT8|A0A5P8PGT8_9CAUD CxxC_CXXC_SSSS domain-containing protein OS=Yersinia phage JC221 OX=2654973 GN=JC221_233 PE=4 SV=1
MM1 pKa = 7.64TYY3 pKa = 11.06DD4 pKa = 4.4DD5 pKa = 4.34MMTFVAAGKK14 pKa = 9.41VAYY17 pKa = 10.38LPVYY21 pKa = 9.03PNVVILRR28 pKa = 11.84EE29 pKa = 3.97GDD31 pKa = 3.58TVIRR35 pKa = 11.84RR36 pKa = 11.84IYY38 pKa = 10.02HH39 pKa = 5.56SPNIDD44 pKa = 3.28MLFIPSSGEE53 pKa = 3.68LRR55 pKa = 11.84TKK57 pKa = 10.23DD58 pKa = 3.41WQIEE62 pKa = 4.37DD63 pKa = 4.05YY64 pKa = 11.41SDD66 pKa = 4.61DD67 pKa = 5.13ANDD70 pKa = 4.25LYY72 pKa = 11.51DD73 pKa = 4.88DD74 pKa = 4.93FVYY77 pKa = 10.96DD78 pKa = 4.11PDD80 pKa = 4.08NFSLQPMEE88 pKa = 4.72IEE90 pKa = 4.08

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8PG94|A0A5P8PG94_9CAUD Uncharacterized protein OS=Yersinia phage JC221 OX=2654973 GN=JC221_021 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.1AYY4 pKa = 10.69NMKK7 pKa = 10.7DD8 pKa = 3.09LVCFNTRR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.57ARR19 pKa = 11.84AYY21 pKa = 10.49VKK23 pKa = 10.59AAGKK27 pKa = 7.88NTSAVIDD34 pKa = 4.35LGTDD38 pKa = 3.16KK39 pKa = 11.31AVGARR44 pKa = 11.84WAAVVVITVAATPVAEE60 pKa = 4.43VKK62 pKa = 9.97TLVLGRR68 pKa = 11.84RR69 pKa = 11.84TEE71 pKa = 4.0TLKK74 pKa = 10.23TSRR77 pKa = 11.84CNNKK81 pKa = 9.81GIHH84 pKa = 6.67DD85 pKa = 3.89VLVVRR90 pKa = 11.84KK91 pKa = 9.33RR92 pKa = 11.84RR93 pKa = 11.84VMEE96 pKa = 3.77VRR98 pKa = 11.84AA99 pKa = 3.86

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

274

0

274

54942

39

1238

200.5

22.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.658 ± 0.161

1.209 ± 0.061

6.565 ± 0.108

6.927 ± 0.16

4.277 ± 0.106

6.126 ± 0.161

1.796 ± 0.075

6.898 ± 0.115

7.526 ± 0.175

7.422 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.072

5.684 ± 0.119

3.567 ± 0.074

3.032 ± 0.109

4.709 ± 0.113

6.203 ± 0.128

5.888 ± 0.191

6.855 ± 0.131

1.31 ± 0.059

4.441 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski