Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) (LRV-1-1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Leishmaniavirus; Leishmania RNA virus 1

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q83096|ORF1_LRVLG Putative ORF1 protein OS=Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) OX=58103 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.57PMSLDD6 pKa = 3.43SLAVGMVALLVDD18 pKa = 4.66YY19 pKa = 10.56YY20 pKa = 11.64SKK22 pKa = 11.47ALRR25 pKa = 11.84ASLNSTGIDD34 pKa = 4.12RR35 pKa = 11.84RR36 pKa = 11.84WGDD39 pKa = 3.23QWPSTVEE46 pKa = 3.51LTGRR50 pKa = 11.84GVIEE54 pKa = 4.09WEE56 pKa = 4.19SPTFCICSSSRR67 pKa = 11.84STAIVV72 pKa = 2.95

Molecular weight: 7.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q83096|ORF1_LRVLG Putative ORF1 protein OS=Leishmania RNA virus 1 - 1 (isolate Leishmania guyanensis) OX=58103 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.16QCPNQNHH8 pKa = 5.52MLVNRR13 pKa = 11.84AMVVAALDD21 pKa = 3.59SFEE24 pKa = 4.17DD25 pKa = 3.26ARR27 pKa = 11.84RR28 pKa = 11.84IISGVLDD35 pKa = 3.97LSRR38 pKa = 11.84LTNTSVKK45 pKa = 10.12GQHH48 pKa = 6.2TNDD51 pKa = 2.94TNYY54 pKa = 9.97FSRR57 pKa = 11.84YY58 pKa = 9.42SNFFSQMPAIILNQLKK74 pKa = 10.31CCIKK78 pKa = 10.69AQVDD82 pKa = 3.83DD83 pKa = 4.07AVKK86 pKa = 10.09MVLQKK91 pKa = 10.77AKK93 pKa = 10.03KK94 pKa = 9.2VEE96 pKa = 3.89VSKK99 pKa = 10.62PVEE102 pKa = 3.93KK103 pKa = 10.37MLTFTTLNTYY113 pKa = 10.4LGYY116 pKa = 9.74PEE118 pKa = 4.33STGCVMEE125 pKa = 4.15YY126 pKa = 10.15TEE128 pKa = 4.48EE129 pKa = 4.01QSGPIAAKK137 pKa = 10.65LLITLLSSTLNAMVRR152 pKa = 11.84PKK154 pKa = 10.46SDD156 pKa = 3.53PNISQNKK163 pKa = 8.27IPRR166 pKa = 11.84HH167 pKa = 4.79YY168 pKa = 9.8YY169 pKa = 7.43QLKK172 pKa = 8.43VHH174 pKa = 6.47VGAQKK179 pKa = 10.45KK180 pKa = 9.86VNLTAIEE187 pKa = 4.33VIRR190 pKa = 11.84GCQHH194 pKa = 5.66EE195 pKa = 4.59CSVVYY200 pKa = 10.58CEE202 pKa = 4.09FVRR205 pKa = 11.84YY206 pKa = 8.79SAYY209 pKa = 10.32FAGLYY214 pKa = 9.93DD215 pKa = 4.16DD216 pKa = 4.43QVAAILLYY224 pKa = 10.65AVAAHH229 pKa = 5.94NVQGFGARR237 pKa = 11.84FCVLWALMCVRR248 pKa = 11.84IAGFADD254 pKa = 5.0DD255 pKa = 3.31INIYY259 pKa = 9.79IKK261 pKa = 10.48HH262 pKa = 6.47RR263 pKa = 11.84GMSGLLPQLVEE274 pKa = 4.36MKK276 pKa = 10.18CLLGRR281 pKa = 11.84GVNEE285 pKa = 3.69IDD287 pKa = 3.62VEE289 pKa = 4.53TEE291 pKa = 3.06ARR293 pKa = 11.84RR294 pKa = 11.84RR295 pKa = 11.84LDD297 pKa = 3.31VGSLSMQRR305 pKa = 11.84LDD307 pKa = 3.83EE308 pKa = 4.48NEE310 pKa = 3.35LRR312 pKa = 11.84AAVRR316 pKa = 11.84LIYY319 pKa = 10.34SEE321 pKa = 4.04EE322 pKa = 3.73LRR324 pKa = 11.84RR325 pKa = 11.84PVTYY329 pKa = 10.15PLICDD334 pKa = 4.53FWSSRR339 pKa = 11.84WLWAANGSHH348 pKa = 6.18SRR350 pKa = 11.84ALEE353 pKa = 4.05HH354 pKa = 6.54AHH356 pKa = 7.1PEE358 pKa = 3.62LATRR362 pKa = 11.84KK363 pKa = 9.35EE364 pKa = 3.84GQAYY368 pKa = 9.18RR369 pKa = 11.84KK370 pKa = 10.0AVMEE374 pKa = 4.05QWQHH378 pKa = 6.38NPMDD382 pKa = 3.62RR383 pKa = 11.84WDD385 pKa = 3.51GTVYY389 pKa = 8.71VTPSAKK395 pKa = 10.15LEE397 pKa = 4.2HH398 pKa = 6.6GKK400 pKa = 7.98TRR402 pKa = 11.84LLLACDD408 pKa = 3.7TLSYY412 pKa = 10.0MWFEE416 pKa = 3.92YY417 pKa = 10.81ALRR420 pKa = 11.84PVEE423 pKa = 4.94RR424 pKa = 11.84IWEE427 pKa = 4.02NSNVILDD434 pKa = 4.11PGSMGNCGIATRR446 pKa = 11.84INGWRR451 pKa = 11.84NGMPGQSFFAVDD463 pKa = 3.44YY464 pKa = 11.42DD465 pKa = 4.57DD466 pKa = 6.21FNSQHH471 pKa = 5.85TLMSQKK477 pKa = 10.04IVFEE481 pKa = 4.21EE482 pKa = 4.33LFHH485 pKa = 7.46HH486 pKa = 6.79IGYY489 pKa = 8.57NASWVKK495 pKa = 9.77TLVDD499 pKa = 3.99SFDD502 pKa = 5.35SMEE505 pKa = 3.9LWIKK509 pKa = 9.08GKK511 pKa = 10.22CAGIMAGTLMSGHH524 pKa = 7.1RR525 pKa = 11.84ATSFINSVLNRR536 pKa = 11.84AYY538 pKa = 9.67IICAGGHH545 pKa = 5.89VPTSMHH551 pKa = 6.21VGDD554 pKa = 6.22DD555 pKa = 3.1ILMSCTLGHH564 pKa = 7.38ADD566 pKa = 4.54NLIANLNRR574 pKa = 11.84KK575 pKa = 7.49GVRR578 pKa = 11.84LNASKK583 pKa = 10.6QVFSKK588 pKa = 10.12TSGEE592 pKa = 4.28FLRR595 pKa = 11.84VAHH598 pKa = 7.0RR599 pKa = 11.84EE600 pKa = 4.36HH601 pKa = 6.78TSHH604 pKa = 6.95GYY606 pKa = 8.27LARR609 pKa = 11.84VISSAVSGNWVSDD622 pKa = 3.2HH623 pKa = 6.44TLNQQEE629 pKa = 4.57ALMNAIVCCRR639 pKa = 11.84GILNRR644 pKa = 11.84SLPGEE649 pKa = 4.16KK650 pKa = 10.33NPVVRR655 pKa = 11.84VISRR659 pKa = 11.84SVSKK663 pKa = 9.68RR664 pKa = 11.84TKK666 pKa = 10.09IEE668 pKa = 3.79EE669 pKa = 3.82KK670 pKa = 10.11TIRR673 pKa = 11.84LLLSGRR679 pKa = 11.84ACLKK683 pKa = 10.22GGVVYY688 pKa = 10.74GEE690 pKa = 3.88QTNYY694 pKa = 8.69IQVYY698 pKa = 9.85RR699 pKa = 11.84INCRR703 pKa = 11.84VEE705 pKa = 3.88RR706 pKa = 11.84SEE708 pKa = 4.78EE709 pKa = 3.68KK710 pKa = 10.65LPPYY714 pKa = 10.25RR715 pKa = 11.84HH716 pKa = 5.69ATEE719 pKa = 6.02DD720 pKa = 3.53YY721 pKa = 10.92LNNHH725 pKa = 6.63LADD728 pKa = 3.64IEE730 pKa = 4.55VMAVRR735 pKa = 11.84QYY737 pKa = 11.7GSDD740 pKa = 3.01IADD743 pKa = 3.14IMAQASWKK751 pKa = 10.46KK752 pKa = 10.93SMSTEE757 pKa = 3.67GAEE760 pKa = 4.21DD761 pKa = 3.63VSRR764 pKa = 11.84LSLQRR769 pKa = 11.84DD770 pKa = 3.19KK771 pKa = 11.05TLPCLHH777 pKa = 7.23CITEE781 pKa = 4.54KK782 pKa = 9.9EE783 pKa = 4.4TSLLPVRR790 pKa = 11.84YY791 pKa = 9.16GLFSSYY797 pKa = 10.12PILMMLKK804 pKa = 9.99DD805 pKa = 4.93RR806 pKa = 11.84IPIKK810 pKa = 10.33EE811 pKa = 4.11ALKK814 pKa = 10.12LAVTIGYY821 pKa = 9.2RR822 pKa = 11.84PQPNSDD828 pKa = 4.25LEE830 pKa = 5.0LDD832 pKa = 3.65LWGEE836 pKa = 4.56SNNSCAIEE844 pKa = 4.4GVLPYY849 pKa = 10.92NEE851 pKa = 4.23ATSLAQKK858 pKa = 9.53LPCGGVVIQVIHH870 pKa = 5.75NVYY873 pKa = 9.98VV874 pKa = 3.24

Molecular weight: 98.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1687

72

874

562.3

62.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.773 ± 1.014

2.845 ± 0.078

5.098 ± 0.65

4.861 ± 0.646

2.845 ± 0.359

6.106 ± 0.125

3.023 ± 0.45

5.513 ± 0.247

3.794 ± 0.794

9.247 ± 0.469

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.142 ± 0.221

4.92 ± 0.552

4.149 ± 0.545

3.142 ± 0.51

6.165 ± 0.165

7.647 ± 1.139

5.868 ± 0.97

7.291 ± 0.51

1.541 ± 0.406

4.031 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski