Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geobacter; Geobacter daltonii

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3798 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B9M0G2|B9M0G2_GEODF Flagellar biogenesis chaperone FlgN OS=Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32) OX=316067 GN=flgN PE=3 SV=1
MM1 pKa = 7.94DD2 pKa = 6.05CDD4 pKa = 3.57GTTTISIVNLEE15 pKa = 4.11TGKK18 pKa = 10.73ASEE21 pKa = 4.71FKK23 pKa = 10.78LFPNEE28 pKa = 3.87SSLTCFVKK36 pKa = 10.64LMNIEE41 pKa = 4.11VIEE44 pKa = 4.34LVDD47 pKa = 5.88LPDD50 pKa = 4.93DD51 pKa = 3.83YY52 pKa = 11.85CEE54 pKa = 4.14VVATGTAMDD63 pKa = 4.02SVGHH67 pKa = 5.99IFKK70 pKa = 10.77VQLLYY75 pKa = 11.11SPEE78 pKa = 3.84TTAEE82 pKa = 4.13KK83 pKa = 10.44EE84 pKa = 3.91QVLADD89 pKa = 3.8IQAGRR94 pKa = 11.84YY95 pKa = 8.4YY96 pKa = 10.52SASGQFSYY104 pKa = 10.97VAEE107 pKa = 4.67EE108 pKa = 4.23GMLMYY113 pKa = 10.23DD114 pKa = 3.94AKK116 pKa = 10.85CRR118 pKa = 11.84EE119 pKa = 4.18LSDD122 pKa = 3.8EE123 pKa = 4.12EE124 pKa = 4.15AAGVIQVFNINEE136 pKa = 4.07MASS139 pKa = 3.17

Molecular weight: 15.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068EZW7|A0A068EZW7_GEODF Uncharacterized protein OS=Geobacter daltonii (strain DSM 22248 / JCM 15807 / FRC-32) OX=316067 GN=Geob_3849 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNVSRR12 pKa = 11.84KK13 pKa = 7.52RR14 pKa = 11.84THH16 pKa = 6.03GFLVRR21 pKa = 11.84MSTKK25 pKa = 10.31NGRR28 pKa = 11.84LVIKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84KK40 pKa = 8.3NLAVSIASKK49 pKa = 10.89

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3798

0

3798

1289234

19

3904

339.5

37.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.248 ± 0.046

1.289 ± 0.02

5.197 ± 0.029

6.368 ± 0.047

4.113 ± 0.032

8.016 ± 0.047

2.045 ± 0.023

6.318 ± 0.037

5.415 ± 0.048

9.954 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.024

3.678 ± 0.04

4.354 ± 0.028

3.251 ± 0.021

5.572 ± 0.04

6.033 ± 0.04

5.561 ± 0.071

7.106 ± 0.032

1.012 ± 0.016

2.918 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski