Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1)
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2794 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E4U0I0|E4U0I0_SULKY Response regulator receiver modulated diguanylate cyclase with PAS/PAC sensor OS=Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1) OX=709032 GN=Sulku_1636 PE=4 SV=1
MM1 pKa = 7.65 DD2 pKa = 4.43 TATPQQIAFILDD14 pKa = 3.33 NVTDD18 pKa = 3.93 YY19 pKa = 11.45 QSLVEE24 pKa = 4.96 GIPEE28 pKa = 4.17 GTPVYY33 pKa = 10.66 VLNNVDD39 pKa = 5.18 DD40 pKa = 4.44 VLAQIAAITADD51 pKa = 3.92 YY52 pKa = 11.21 SNLDD56 pKa = 4.17 AIHH59 pKa = 6.58 LLSHH63 pKa = 6.74 GSDD66 pKa = 3.36 GSINLGTFSLNSDD79 pKa = 4.0 NLNTYY84 pKa = 11.04 ADD86 pKa = 3.69 TLGQIGASLTEE97 pKa = 4.43 HH98 pKa = 7.14 GDD100 pKa = 3.16 ILLYY104 pKa = 10.03 GCNVARR110 pKa = 11.84 DD111 pKa = 3.45 QSGIDD116 pKa = 3.76 FIGKK120 pKa = 8.99 LAQLSGADD128 pKa = 3.14 IAASNDD134 pKa = 3.26 MTGSNTQNGDD144 pKa = 3.58 WILEE148 pKa = 4.12 SSIGMIDD155 pKa = 3.51 TPALVLNQYY164 pKa = 9.56 NQTLAAGTVTFTSSPSAATATDD186 pKa = 3.73 GTASNDD192 pKa = 2.91 IAGIVLNFTSDD203 pKa = 3.94 LGTNWTYY210 pKa = 7.38 EE211 pKa = 4.03 TPYY214 pKa = 8.64 TGSVITVGYY223 pKa = 7.85 ATNAPQALMTIKK235 pKa = 10.7 SSDD238 pKa = 3.19 GSNFWFKK245 pKa = 11.42 SLFIADD251 pKa = 3.83 SGGQPVKK258 pKa = 11.24 VEE260 pKa = 3.96 GFEE263 pKa = 4.68 NNVSTGSVNLDD274 pKa = 3.33 TTGGGSLYY282 pKa = 10.67 EE283 pKa = 4.19 NTFGTASLTQSIFQNVDD300 pKa = 2.86 EE301 pKa = 4.86 VRR303 pKa = 11.84 ITPQSGVQMWIGVNNIQIDD322 pKa = 4.12 NPVLPGPTVTSALYY336 pKa = 10.22 DD337 pKa = 3.4 ASTNSLVVTGTNMTATAGVTNDD359 pKa = 2.9 INVAKK364 pKa = 9.37 LTLTGEE370 pKa = 4.37 GGATYY375 pKa = 9.95 TLTSSNVEE383 pKa = 3.73 IDD385 pKa = 3.2 SATQFTVALNATDD398 pKa = 3.69 QINVEE403 pKa = 4.0 GLLNKK408 pKa = 10.43 NGTSSVGTTTYY419 pKa = 10.9 NIAAAADD426 pKa = 3.5 WDD428 pKa = 4.19 VASTGNADD436 pKa = 3.44 LTGNGITVSNTQTPTITSATYY457 pKa = 10.28 DD458 pKa = 3.45 AATGSLVVTGTNLVKK473 pKa = 8.89 TTGATNDD480 pKa = 2.94 ITVNKK485 pKa = 8.93 LTFTGEE491 pKa = 4.0 GGSTYY496 pKa = 10.6 TLTSSNVEE504 pKa = 3.77 ITDD507 pKa = 3.37 ATSFSVTLNATDD519 pKa = 4.66 LAALNQIVNKK529 pKa = 10.28 NGTSSTGATTYY540 pKa = 11.38 NLAAADD546 pKa = 4.09 DD547 pKa = 4.34 WNSVITAGTIADD559 pKa = 3.6 ATSAVTASNVAVPAITNATYY579 pKa = 10.47 DD580 pKa = 3.41 ASTGALVVTGTGFLKK595 pKa = 10.96 LSGATNDD602 pKa = 2.83 IVANKK607 pKa = 8.26 FTFTGEE613 pKa = 3.66 GGATYY618 pKa = 10.2 TLTDD622 pKa = 3.52 TSNVEE627 pKa = 4.09 ITSGTAFTITLSATDD642 pKa = 3.78 KK643 pKa = 11.15 AALNLMMDD651 pKa = 3.84 KK652 pKa = 10.92 NGTSSTSGTTYY663 pKa = 11.3 NLAAAEE669 pKa = 4.06 DD670 pKa = 3.91 WAAGADD676 pKa = 3.67 AAVVVADD683 pKa = 3.76 TTGNGVTASNVAVPTITSSTYY704 pKa = 9.72 NASTGALVVTGTGFLSKK721 pKa = 10.63 SGATNDD727 pKa = 2.69 IVANKK732 pKa = 8.26 FTFTGEE738 pKa = 3.66 GGATYY743 pKa = 10.2 TLTDD747 pKa = 3.52 TSNVEE752 pKa = 3.93 ITSGTVFTITLSATDD767 pKa = 4.1 LAGVDD772 pKa = 3.95 QIMNKK777 pKa = 10.09 NGTASTSGTTYY788 pKa = 11.3 NLAAAEE794 pKa = 4.27 DD795 pKa = 4.02 WASGADD801 pKa = 3.49 AAVVVADD808 pKa = 3.76 TTGNGVTASNVAVPTITSATYY829 pKa = 10.15 DD830 pKa = 3.54 YY831 pKa = 8.69 NTNVLTVTGTGFLNKK846 pKa = 10.07 SGATNDD852 pKa = 2.97 IDD854 pKa = 3.98 LTKK857 pKa = 10.2 LTFSGEE863 pKa = 4.13 GGSTYY868 pKa = 10.77 TLTNATGVEE877 pKa = 4.3 ITSGTSFSVTLSGADD892 pKa = 3.96 LINVEE897 pKa = 4.3 TLLNKK902 pKa = 10.41 NGTSAVSGTTYY913 pKa = 11.3 NLAAAEE919 pKa = 4.06 DD920 pKa = 3.91 WAAGADD926 pKa = 3.67 AAVVVADD933 pKa = 3.85 TTGNGITLSNYY944 pKa = 8.44 AAPTVTSATYY954 pKa = 10.34 DD955 pKa = 3.04 AGTHH959 pKa = 4.84 VLSVTGTNLVSKK971 pKa = 10.72 SGATNDD977 pKa = 3.49 VAVSMLTVTGEE988 pKa = 3.96 GGTYY992 pKa = 9.67 TLTSSDD998 pKa = 3.36 VDD1000 pKa = 3.25 ITNATTFSVTLNAADD1015 pKa = 3.46 QLVVNGLLNKK1025 pKa = 10.44 NGTASSGATTYY1036 pKa = 11.46 NLAAAEE1042 pKa = 4.14 DD1043 pKa = 4.21 WLAGTASATVVADD1056 pKa = 3.44 LTGNGITVSNVQTPTITSATYY1077 pKa = 10.77 DD1078 pKa = 3.64 STTGSLVVTGTNFFSKK1094 pKa = 10.58 VGANNDD1100 pKa = 2.86 IDD1102 pKa = 4.22 LTKK1105 pKa = 10.39 LTFTGGAANATYY1117 pKa = 9.71 TLSSTSNVEE1126 pKa = 3.67 ITSATSFSITLSGADD1141 pKa = 3.58 KK1142 pKa = 10.71 TSVDD1146 pKa = 3.97 ALLDD1150 pKa = 4.3 NIGTTSSGGSTYY1162 pKa = 11.36 NLAAANNWLAGADD1175 pKa = 3.61 SAAVITDD1182 pKa = 3.74 AVNAITVSINPAITSATYY1200 pKa = 10.2 DD1201 pKa = 3.23 ASTGSLVVTGTNMQVMAGAANDD1223 pKa = 3.19 ITANKK1228 pKa = 8.7 LTFTGEE1234 pKa = 3.65 GGATYY1239 pKa = 10.15 TLTDD1243 pKa = 3.5 SANVEE1248 pKa = 4.17 LSSATSFTIALSATDD1263 pKa = 3.83 KK1264 pKa = 11.25 AAINQIVNKK1273 pKa = 9.96 NGTSSTGATTYY1284 pKa = 11.38 NLAAADD1290 pKa = 3.73 DD1291 pKa = 4.16 WNAQVSAGDD1300 pKa = 3.57 TSDD1303 pKa = 2.98 ATNAVTVSSVAAPTITNATYY1323 pKa = 10.44 DD1324 pKa = 3.59 ASTGALVVTGTGFLKK1339 pKa = 10.96 LSGATNDD1346 pKa = 2.83 IVANKK1351 pKa = 8.26 FTFTGEE1357 pKa = 3.66 GGATYY1362 pKa = 10.2 TLTDD1366 pKa = 3.52 TSNVEE1371 pKa = 4.09 ITSGTAFTITLSATDD1386 pKa = 3.78 KK1387 pKa = 11.15 AALNLMMDD1395 pKa = 3.84 KK1396 pKa = 10.92 NGTSSTSGTTYY1407 pKa = 11.3 NLAAAEE1413 pKa = 4.06 DD1414 pKa = 3.91 WAAGADD1420 pKa = 3.67 AAVVVADD1427 pKa = 3.76 TTGNGVTASNVAVPTITSSTYY1448 pKa = 9.72 NASTGALVVTGTGFLSKK1465 pKa = 10.63 SGATNDD1471 pKa = 2.69 IVANKK1476 pKa = 8.26 FTFTGEE1482 pKa = 3.66 GGATYY1487 pKa = 10.2 TLTDD1491 pKa = 3.52 TSNVEE1496 pKa = 3.93 ITSGTVFTITLSATDD1511 pKa = 4.1 LAGVDD1516 pKa = 3.95 QIMNKK1521 pKa = 10.09 NGTASTSGTTYY1532 pKa = 11.3 NLAAAEE1538 pKa = 4.27 DD1539 pKa = 4.02 WASGADD1545 pKa = 3.49 AAVVVADD1552 pKa = 3.76 TTGNGVTASNVAVPAITSSTYY1573 pKa = 10.09 NASTGALVVTGTGFLKK1589 pKa = 10.96 LSGATNDD1596 pKa = 2.83 IVANKK1601 pKa = 8.26 FTFTGEE1607 pKa = 3.66 GGATYY1612 pKa = 10.2 TLTDD1616 pKa = 3.52 TSNVEE1621 pKa = 4.09 ITSGTAFTITLSATDD1636 pKa = 3.78 KK1637 pKa = 11.15 AALNLMMDD1645 pKa = 3.84 KK1646 pKa = 10.92 NGTSSTSGTTYY1657 pKa = 11.3 NLAAAEE1663 pKa = 4.06 DD1664 pKa = 3.91 WAAGADD1670 pKa = 3.67 AAVVVADD1677 pKa = 3.76 TTGNGVTASNVAVPTITSSTYY1698 pKa = 9.72 NASTGALVVTGTGFLSKK1715 pKa = 10.63 SGATNDD1721 pKa = 2.69 IVANKK1726 pKa = 8.26 FTFTGEE1732 pKa = 3.66 GGATYY1737 pKa = 10.2 TLTDD1741 pKa = 3.52 TSNVEE1746 pKa = 3.93 ITSGTVFTITLSATDD1761 pKa = 4.1 LAGVDD1766 pKa = 3.95 QIMNKK1771 pKa = 10.09 NGTASTSGTTYY1782 pKa = 11.3 NLAAAEE1788 pKa = 4.27 DD1789 pKa = 4.02 WASGADD1795 pKa = 3.49 AAVVVADD1802 pKa = 3.76 TTGNGVTASNVAVPTITSATYY1823 pKa = 10.5 DD1824 pKa = 3.25 SGTGVLTVSGTHH1836 pKa = 5.88 FVHH1839 pKa = 7.59 KK1840 pKa = 10.46 SGSTNDD1846 pKa = 3.44 IDD1848 pKa = 6.19 LSMLTFTGEE1857 pKa = 3.7 AGGTYY1862 pKa = 9.82 TLTTATDD1869 pKa = 3.53 VEE1871 pKa = 4.73 ITSGTSFTVTLSGADD1886 pKa = 3.31 KK1887 pKa = 10.74 TGVDD1891 pKa = 3.42 TLLNKK1896 pKa = 10.11 IGTSSTDD1903 pKa = 2.72 ATTYY1907 pKa = 11.2 NLAAAEE1913 pKa = 4.12 DD1914 pKa = 4.16 WATGADD1920 pKa = 3.26 AAVNVVDD1927 pKa = 3.91 ATNAITVSQTIASSGGGGGTSTPTTTIDD1955 pKa = 3.24 GATVEE1960 pKa = 4.62 TTTATNSSGQTVEE1973 pKa = 4.32 QIIILPISSTRR1984 pKa = 11.84 VNSTGDD1990 pKa = 3.05 TATADD1995 pKa = 3.22 IPLFWGEE2002 pKa = 3.87 SSRR2005 pKa = 11.84 TVDD2008 pKa = 2.86 ATTANLPTGIGLSATGARR2026 pKa = 11.84 APQSGHH2032 pKa = 6.45 TIQDD2036 pKa = 3.83 SINDD2040 pKa = 3.94 LLALINEE2047 pKa = 4.78 TAPSTDD2053 pKa = 3.04 TSKK2056 pKa = 10.17 TSMLGGGSTFLNTLSGITDD2075 pKa = 3.25 NLVINKK2081 pKa = 7.94 ITLTHH2086 pKa = 6.96 TGTSAPGMPITIQGTTSTVTTSGGALPPVEE2116 pKa = 4.76 ALVIDD2121 pKa = 4.47 GQTLLSNTTLSLNNVEE2137 pKa = 4.28 FATIIGEE2144 pKa = 4.0 NLIIRR2149 pKa = 11.84 GGEE2152 pKa = 3.79 GRR2154 pKa = 11.84 NTIFTGDD2161 pKa = 3.24 GHH2163 pKa = 6.8 QDD2165 pKa = 3.12 IMCGTDD2171 pKa = 3.85 DD2172 pKa = 5.74 DD2173 pKa = 4.51 EE2174 pKa = 4.61 LHH2176 pKa = 7.08 AGGGDD2181 pKa = 3.67 DD2182 pKa = 3.82 TVGSAGGNDD2191 pKa = 3.83 RR2192 pKa = 11.84 IFAEE2196 pKa = 4.64 AGNDD2200 pKa = 3.54 TVFGGTGDD2208 pKa = 4.27 DD2209 pKa = 4.03 FMHH2212 pKa = 6.75 GGSGNDD2218 pKa = 2.96 TVTYY2222 pKa = 10.2 DD2223 pKa = 3.79 GNRR2226 pKa = 11.84 GDD2228 pKa = 4.28 YY2229 pKa = 10.94 VITRR2233 pKa = 11.84 DD2234 pKa = 3.24 NGKK2237 pKa = 7.74 TYY2239 pKa = 10.75 VYY2241 pKa = 9.47 TVSTGEE2247 pKa = 3.8 TDD2249 pKa = 3.49 TVVNAEE2255 pKa = 4.49 SIQFADD2261 pKa = 4.91 AIYY2264 pKa = 9.56 TITNNEE2270 pKa = 4.71 DD2271 pKa = 3.02 LTQIASLYY2279 pKa = 8.19 QQILGRR2285 pKa = 11.84 QADD2288 pKa = 4.02 LDD2290 pKa = 3.88 GFQYY2294 pKa = 7.64 WTSTFDD2300 pKa = 3.27 NGDD2303 pKa = 3.75 TIGAIATSFVRR2314 pKa = 11.84 SSEE2317 pKa = 4.06 YY2318 pKa = 10.85 FNDD2321 pKa = 3.7 TGNVWDD2327 pKa = 5.19 SMSNDD2332 pKa = 3.01 QRR2334 pKa = 11.84 IEE2336 pKa = 3.83 TFYY2339 pKa = 11.42 QLMLGRR2345 pKa = 11.84 ASDD2348 pKa = 4.34 AVGKK2352 pKa = 9.94 AYY2354 pKa = 9.76 WMDD2357 pKa = 5.06 AINNGTMSIQDD2368 pKa = 3.25 IAGSFVEE2375 pKa = 4.24 SSEE2378 pKa = 3.83 MQGIYY2383 pKa = 9.62 TGQQAWSFFVV2393 pKa = 3.18
Molecular weight: 241.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.617
IPC_protein 3.694
Toseland 3.439
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.503
Grimsley 3.35
Solomon 3.694
Lehninger 3.643
Nozaki 3.795
DTASelect 4.088
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.808
Patrickios 1.659
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|E4U0N6|E4U0N6_SULKY pEK499_p136 domain-containing protein OS=Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1) OX=709032 GN=Sulku_1692 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.04 QPHH8 pKa = 4.86 NTPRR12 pKa = 11.84 KK13 pKa = 7.32 RR14 pKa = 11.84 THH16 pKa = 6.0 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.15 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2794
0
2794
867431
30
3343
310.5
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.729 ± 0.05
0.904 ± 0.017
5.551 ± 0.039
7.081 ± 0.047
4.584 ± 0.039
6.386 ± 0.05
2.313 ± 0.025
7.913 ± 0.043
6.467 ± 0.046
9.819 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.776 ± 0.026
4.539 ± 0.041
3.406 ± 0.03
3.225 ± 0.026
4.377 ± 0.034
6.79 ± 0.034
5.403 ± 0.056
6.186 ± 0.037
0.847 ± 0.015
3.705 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here