Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Thiovulaceae; Sulfuricurvum; Sulfuricurvum kujiense

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2794 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|E4U0I0|E4U0I0_SULKY Response regulator receiver modulated diguanylate cyclase with PAS/PAC sensor OS=Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1) OX=709032 GN=Sulku_1636 PE=4 SV=1
MM1 pKa = 7.65DD2 pKa = 4.43TATPQQIAFILDD14 pKa = 3.33NVTDD18 pKa = 3.93YY19 pKa = 11.45QSLVEE24 pKa = 4.96GIPEE28 pKa = 4.17GTPVYY33 pKa = 10.66VLNNVDD39 pKa = 5.18DD40 pKa = 4.44VLAQIAAITADD51 pKa = 3.92YY52 pKa = 11.21SNLDD56 pKa = 4.17AIHH59 pKa = 6.58LLSHH63 pKa = 6.74GSDD66 pKa = 3.36GSINLGTFSLNSDD79 pKa = 4.0NLNTYY84 pKa = 11.04ADD86 pKa = 3.69TLGQIGASLTEE97 pKa = 4.43HH98 pKa = 7.14GDD100 pKa = 3.16ILLYY104 pKa = 10.03GCNVARR110 pKa = 11.84DD111 pKa = 3.45QSGIDD116 pKa = 3.76FIGKK120 pKa = 8.99LAQLSGADD128 pKa = 3.14IAASNDD134 pKa = 3.26MTGSNTQNGDD144 pKa = 3.58WILEE148 pKa = 4.12SSIGMIDD155 pKa = 3.51TPALVLNQYY164 pKa = 9.56NQTLAAGTVTFTSSPSAATATDD186 pKa = 3.73GTASNDD192 pKa = 2.91IAGIVLNFTSDD203 pKa = 3.94LGTNWTYY210 pKa = 7.38EE211 pKa = 4.03TPYY214 pKa = 8.64TGSVITVGYY223 pKa = 7.85ATNAPQALMTIKK235 pKa = 10.7SSDD238 pKa = 3.19GSNFWFKK245 pKa = 11.42SLFIADD251 pKa = 3.83SGGQPVKK258 pKa = 11.24VEE260 pKa = 3.96GFEE263 pKa = 4.68NNVSTGSVNLDD274 pKa = 3.33TTGGGSLYY282 pKa = 10.67EE283 pKa = 4.19NTFGTASLTQSIFQNVDD300 pKa = 2.86EE301 pKa = 4.86VRR303 pKa = 11.84ITPQSGVQMWIGVNNIQIDD322 pKa = 4.12NPVLPGPTVTSALYY336 pKa = 10.22DD337 pKa = 3.4ASTNSLVVTGTNMTATAGVTNDD359 pKa = 2.9INVAKK364 pKa = 9.37LTLTGEE370 pKa = 4.37GGATYY375 pKa = 9.95TLTSSNVEE383 pKa = 3.73IDD385 pKa = 3.2SATQFTVALNATDD398 pKa = 3.69QINVEE403 pKa = 4.0GLLNKK408 pKa = 10.43NGTSSVGTTTYY419 pKa = 10.9NIAAAADD426 pKa = 3.5WDD428 pKa = 4.19VASTGNADD436 pKa = 3.44LTGNGITVSNTQTPTITSATYY457 pKa = 10.28DD458 pKa = 3.45AATGSLVVTGTNLVKK473 pKa = 8.89TTGATNDD480 pKa = 2.94ITVNKK485 pKa = 8.93LTFTGEE491 pKa = 4.0GGSTYY496 pKa = 10.6TLTSSNVEE504 pKa = 3.77ITDD507 pKa = 3.37ATSFSVTLNATDD519 pKa = 4.66LAALNQIVNKK529 pKa = 10.28NGTSSTGATTYY540 pKa = 11.38NLAAADD546 pKa = 4.09DD547 pKa = 4.34WNSVITAGTIADD559 pKa = 3.6ATSAVTASNVAVPAITNATYY579 pKa = 10.47DD580 pKa = 3.41ASTGALVVTGTGFLKK595 pKa = 10.96LSGATNDD602 pKa = 2.83IVANKK607 pKa = 8.26FTFTGEE613 pKa = 3.66GGATYY618 pKa = 10.2TLTDD622 pKa = 3.52TSNVEE627 pKa = 4.09ITSGTAFTITLSATDD642 pKa = 3.78KK643 pKa = 11.15AALNLMMDD651 pKa = 3.84KK652 pKa = 10.92NGTSSTSGTTYY663 pKa = 11.3NLAAAEE669 pKa = 4.06DD670 pKa = 3.91WAAGADD676 pKa = 3.67AAVVVADD683 pKa = 3.76TTGNGVTASNVAVPTITSSTYY704 pKa = 9.72NASTGALVVTGTGFLSKK721 pKa = 10.63SGATNDD727 pKa = 2.69IVANKK732 pKa = 8.26FTFTGEE738 pKa = 3.66GGATYY743 pKa = 10.2TLTDD747 pKa = 3.52TSNVEE752 pKa = 3.93ITSGTVFTITLSATDD767 pKa = 4.1LAGVDD772 pKa = 3.95QIMNKK777 pKa = 10.09NGTASTSGTTYY788 pKa = 11.3NLAAAEE794 pKa = 4.27DD795 pKa = 4.02WASGADD801 pKa = 3.49AAVVVADD808 pKa = 3.76TTGNGVTASNVAVPTITSATYY829 pKa = 10.15DD830 pKa = 3.54YY831 pKa = 8.69NTNVLTVTGTGFLNKK846 pKa = 10.07SGATNDD852 pKa = 2.97IDD854 pKa = 3.98LTKK857 pKa = 10.2LTFSGEE863 pKa = 4.13GGSTYY868 pKa = 10.77TLTNATGVEE877 pKa = 4.3ITSGTSFSVTLSGADD892 pKa = 3.96LINVEE897 pKa = 4.3TLLNKK902 pKa = 10.41NGTSAVSGTTYY913 pKa = 11.3NLAAAEE919 pKa = 4.06DD920 pKa = 3.91WAAGADD926 pKa = 3.67AAVVVADD933 pKa = 3.85TTGNGITLSNYY944 pKa = 8.44AAPTVTSATYY954 pKa = 10.34DD955 pKa = 3.04AGTHH959 pKa = 4.84VLSVTGTNLVSKK971 pKa = 10.72SGATNDD977 pKa = 3.49VAVSMLTVTGEE988 pKa = 3.96GGTYY992 pKa = 9.67TLTSSDD998 pKa = 3.36VDD1000 pKa = 3.25ITNATTFSVTLNAADD1015 pKa = 3.46QLVVNGLLNKK1025 pKa = 10.44NGTASSGATTYY1036 pKa = 11.46NLAAAEE1042 pKa = 4.14DD1043 pKa = 4.21WLAGTASATVVADD1056 pKa = 3.44LTGNGITVSNVQTPTITSATYY1077 pKa = 10.77DD1078 pKa = 3.64STTGSLVVTGTNFFSKK1094 pKa = 10.58VGANNDD1100 pKa = 2.86IDD1102 pKa = 4.22LTKK1105 pKa = 10.39LTFTGGAANATYY1117 pKa = 9.71TLSSTSNVEE1126 pKa = 3.67ITSATSFSITLSGADD1141 pKa = 3.58KK1142 pKa = 10.71TSVDD1146 pKa = 3.97ALLDD1150 pKa = 4.3NIGTTSSGGSTYY1162 pKa = 11.36NLAAANNWLAGADD1175 pKa = 3.61SAAVITDD1182 pKa = 3.74AVNAITVSINPAITSATYY1200 pKa = 10.2DD1201 pKa = 3.23ASTGSLVVTGTNMQVMAGAANDD1223 pKa = 3.19ITANKK1228 pKa = 8.7LTFTGEE1234 pKa = 3.65GGATYY1239 pKa = 10.15TLTDD1243 pKa = 3.5SANVEE1248 pKa = 4.17LSSATSFTIALSATDD1263 pKa = 3.83KK1264 pKa = 11.25AAINQIVNKK1273 pKa = 9.96NGTSSTGATTYY1284 pKa = 11.38NLAAADD1290 pKa = 3.73DD1291 pKa = 4.16WNAQVSAGDD1300 pKa = 3.57TSDD1303 pKa = 2.98ATNAVTVSSVAAPTITNATYY1323 pKa = 10.44DD1324 pKa = 3.59ASTGALVVTGTGFLKK1339 pKa = 10.96LSGATNDD1346 pKa = 2.83IVANKK1351 pKa = 8.26FTFTGEE1357 pKa = 3.66GGATYY1362 pKa = 10.2TLTDD1366 pKa = 3.52TSNVEE1371 pKa = 4.09ITSGTAFTITLSATDD1386 pKa = 3.78KK1387 pKa = 11.15AALNLMMDD1395 pKa = 3.84KK1396 pKa = 10.92NGTSSTSGTTYY1407 pKa = 11.3NLAAAEE1413 pKa = 4.06DD1414 pKa = 3.91WAAGADD1420 pKa = 3.67AAVVVADD1427 pKa = 3.76TTGNGVTASNVAVPTITSSTYY1448 pKa = 9.72NASTGALVVTGTGFLSKK1465 pKa = 10.63SGATNDD1471 pKa = 2.69IVANKK1476 pKa = 8.26FTFTGEE1482 pKa = 3.66GGATYY1487 pKa = 10.2TLTDD1491 pKa = 3.52TSNVEE1496 pKa = 3.93ITSGTVFTITLSATDD1511 pKa = 4.1LAGVDD1516 pKa = 3.95QIMNKK1521 pKa = 10.09NGTASTSGTTYY1532 pKa = 11.3NLAAAEE1538 pKa = 4.27DD1539 pKa = 4.02WASGADD1545 pKa = 3.49AAVVVADD1552 pKa = 3.76TTGNGVTASNVAVPAITSSTYY1573 pKa = 10.09NASTGALVVTGTGFLKK1589 pKa = 10.96LSGATNDD1596 pKa = 2.83IVANKK1601 pKa = 8.26FTFTGEE1607 pKa = 3.66GGATYY1612 pKa = 10.2TLTDD1616 pKa = 3.52TSNVEE1621 pKa = 4.09ITSGTAFTITLSATDD1636 pKa = 3.78KK1637 pKa = 11.15AALNLMMDD1645 pKa = 3.84KK1646 pKa = 10.92NGTSSTSGTTYY1657 pKa = 11.3NLAAAEE1663 pKa = 4.06DD1664 pKa = 3.91WAAGADD1670 pKa = 3.67AAVVVADD1677 pKa = 3.76TTGNGVTASNVAVPTITSSTYY1698 pKa = 9.72NASTGALVVTGTGFLSKK1715 pKa = 10.63SGATNDD1721 pKa = 2.69IVANKK1726 pKa = 8.26FTFTGEE1732 pKa = 3.66GGATYY1737 pKa = 10.2TLTDD1741 pKa = 3.52TSNVEE1746 pKa = 3.93ITSGTVFTITLSATDD1761 pKa = 4.1LAGVDD1766 pKa = 3.95QIMNKK1771 pKa = 10.09NGTASTSGTTYY1782 pKa = 11.3NLAAAEE1788 pKa = 4.27DD1789 pKa = 4.02WASGADD1795 pKa = 3.49AAVVVADD1802 pKa = 3.76TTGNGVTASNVAVPTITSATYY1823 pKa = 10.5DD1824 pKa = 3.25SGTGVLTVSGTHH1836 pKa = 5.88FVHH1839 pKa = 7.59KK1840 pKa = 10.46SGSTNDD1846 pKa = 3.44IDD1848 pKa = 6.19LSMLTFTGEE1857 pKa = 3.7AGGTYY1862 pKa = 9.82TLTTATDD1869 pKa = 3.53VEE1871 pKa = 4.73ITSGTSFTVTLSGADD1886 pKa = 3.31KK1887 pKa = 10.74TGVDD1891 pKa = 3.42TLLNKK1896 pKa = 10.11IGTSSTDD1903 pKa = 2.72ATTYY1907 pKa = 11.2NLAAAEE1913 pKa = 4.12DD1914 pKa = 4.16WATGADD1920 pKa = 3.26AAVNVVDD1927 pKa = 3.91ATNAITVSQTIASSGGGGGTSTPTTTIDD1955 pKa = 3.24GATVEE1960 pKa = 4.62TTTATNSSGQTVEE1973 pKa = 4.32QIIILPISSTRR1984 pKa = 11.84VNSTGDD1990 pKa = 3.05TATADD1995 pKa = 3.22IPLFWGEE2002 pKa = 3.87SSRR2005 pKa = 11.84TVDD2008 pKa = 2.86ATTANLPTGIGLSATGARR2026 pKa = 11.84APQSGHH2032 pKa = 6.45TIQDD2036 pKa = 3.83SINDD2040 pKa = 3.94LLALINEE2047 pKa = 4.78TAPSTDD2053 pKa = 3.04TSKK2056 pKa = 10.17TSMLGGGSTFLNTLSGITDD2075 pKa = 3.25NLVINKK2081 pKa = 7.94ITLTHH2086 pKa = 6.96TGTSAPGMPITIQGTTSTVTTSGGALPPVEE2116 pKa = 4.76ALVIDD2121 pKa = 4.47GQTLLSNTTLSLNNVEE2137 pKa = 4.28FATIIGEE2144 pKa = 4.0NLIIRR2149 pKa = 11.84GGEE2152 pKa = 3.79GRR2154 pKa = 11.84NTIFTGDD2161 pKa = 3.24GHH2163 pKa = 6.8QDD2165 pKa = 3.12IMCGTDD2171 pKa = 3.85DD2172 pKa = 5.74DD2173 pKa = 4.51EE2174 pKa = 4.61LHH2176 pKa = 7.08AGGGDD2181 pKa = 3.67DD2182 pKa = 3.82TVGSAGGNDD2191 pKa = 3.83RR2192 pKa = 11.84IFAEE2196 pKa = 4.64AGNDD2200 pKa = 3.54TVFGGTGDD2208 pKa = 4.27DD2209 pKa = 4.03FMHH2212 pKa = 6.75GGSGNDD2218 pKa = 2.96TVTYY2222 pKa = 10.2DD2223 pKa = 3.79GNRR2226 pKa = 11.84GDD2228 pKa = 4.28YY2229 pKa = 10.94VITRR2233 pKa = 11.84DD2234 pKa = 3.24NGKK2237 pKa = 7.74TYY2239 pKa = 10.75VYY2241 pKa = 9.47TVSTGEE2247 pKa = 3.8TDD2249 pKa = 3.49TVVNAEE2255 pKa = 4.49SIQFADD2261 pKa = 4.91AIYY2264 pKa = 9.56TITNNEE2270 pKa = 4.71DD2271 pKa = 3.02LTQIASLYY2279 pKa = 8.19QQILGRR2285 pKa = 11.84QADD2288 pKa = 4.02LDD2290 pKa = 3.88GFQYY2294 pKa = 7.64WTSTFDD2300 pKa = 3.27NGDD2303 pKa = 3.75TIGAIATSFVRR2314 pKa = 11.84SSEE2317 pKa = 4.06YY2318 pKa = 10.85FNDD2321 pKa = 3.7TGNVWDD2327 pKa = 5.19SMSNDD2332 pKa = 3.01QRR2334 pKa = 11.84IEE2336 pKa = 3.83TFYY2339 pKa = 11.42QLMLGRR2345 pKa = 11.84ASDD2348 pKa = 4.34AVGKK2352 pKa = 9.94AYY2354 pKa = 9.76WMDD2357 pKa = 5.06AINNGTMSIQDD2368 pKa = 3.25IAGSFVEE2375 pKa = 4.24SSEE2378 pKa = 3.83MQGIYY2383 pKa = 9.62TGQQAWSFFVV2393 pKa = 3.18

Molecular weight:
241.54 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|E4U0N6|E4U0N6_SULKY pEK499_p136 domain-containing protein OS=Sulfuricurvum kujiense (strain ATCC BAA-921 / DSM 16994 / JCM 11577 / YK-1) OX=709032 GN=Sulku_1692 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.0GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.15NGRR28 pKa = 11.84RR29 pKa = 11.84VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2794

0

2794

867431

30

3343

310.5

34.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.729 ± 0.05

0.904 ± 0.017

5.551 ± 0.039

7.081 ± 0.047

4.584 ± 0.039

6.386 ± 0.05

2.313 ± 0.025

7.913 ± 0.043

6.467 ± 0.046

9.819 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.776 ± 0.026

4.539 ± 0.041

3.406 ± 0.03

3.225 ± 0.026

4.377 ± 0.034

6.79 ± 0.034

5.403 ± 0.056

6.186 ± 0.037

0.847 ± 0.015

3.705 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski