Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfocapsa; Desulfocapsa sulfexigens

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1PGR0|M1PGR0_DESSD 3-dehydroquinate synthase OS=Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1) OX=1167006 GN=aroB PE=3 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84KK3 pKa = 9.21IVILVTCCFLLGFSNRR19 pKa = 11.84VHH21 pKa = 7.2ALEE24 pKa = 4.32ILYY27 pKa = 10.59NDD29 pKa = 4.37LSGGGINAIQMSAFNEE45 pKa = 3.99AASYY49 pKa = 9.31WEE51 pKa = 4.88SIFVDD56 pKa = 3.55NVTVRR61 pKa = 11.84VDD63 pKa = 3.3IDD65 pKa = 3.52MQALGTGIIGSAQSYY80 pKa = 9.93KK81 pKa = 10.29VDD83 pKa = 3.38TYY85 pKa = 11.71YY86 pKa = 11.68GDD88 pKa = 3.35IRR90 pKa = 11.84NALAGDD96 pKa = 3.68VRR98 pKa = 11.84STDD101 pKa = 4.78DD102 pKa = 4.43ILATSNLQTTDD113 pKa = 2.94HH114 pKa = 6.68LNVVVNDD121 pKa = 4.8LYY123 pKa = 11.39DD124 pKa = 5.18DD125 pKa = 3.52NHH127 pKa = 7.03AWILNSSSTVEE138 pKa = 4.19VNSEE142 pKa = 3.79LWVNRR147 pKa = 11.84ANLKK151 pKa = 10.31ALSLLNDD158 pKa = 4.73DD159 pKa = 4.58GQADD163 pKa = 3.95ASVLMSSNFSFDD175 pKa = 3.26YY176 pKa = 10.82DD177 pKa = 3.84RR178 pKa = 11.84SDD180 pKa = 5.52GIDD183 pKa = 3.41SDD185 pKa = 4.11KK186 pKa = 11.46LDD188 pKa = 3.63FAGVMVHH195 pKa = 7.03EE196 pKa = 5.38IGHH199 pKa = 6.34ALGFVSGVDD208 pKa = 2.84IMDD211 pKa = 3.62YY212 pKa = 10.98YY213 pKa = 11.58GLDD216 pKa = 3.65GPGNGSWDD224 pKa = 3.6WGWSGEE230 pKa = 4.17GSSNTLEE237 pKa = 4.25DD238 pKa = 2.85EE239 pKa = 5.21GYY241 pKa = 10.88FSVLDD246 pKa = 3.56LFRR249 pKa = 11.84YY250 pKa = 9.5SDD252 pKa = 4.14YY253 pKa = 11.12INEE256 pKa = 4.22YY257 pKa = 10.43EE258 pKa = 4.18SGSLFGYY265 pKa = 10.51LDD267 pKa = 3.18WTYY270 pKa = 11.45EE271 pKa = 4.2SVEE274 pKa = 4.29GGWGDD279 pKa = 3.73SYY281 pKa = 11.81FSFDD285 pKa = 4.37GGTTNLAPFSTGRR298 pKa = 11.84YY299 pKa = 9.31NGDD302 pKa = 3.52GYY304 pKa = 10.82QASHH308 pKa = 6.74WEE310 pKa = 4.13DD311 pKa = 3.59DD312 pKa = 3.86LGLGRR317 pKa = 11.84MDD319 pKa = 3.43PTTAYY324 pKa = 10.47GEE326 pKa = 4.45YY327 pKa = 10.49YY328 pKa = 10.83DD329 pKa = 5.78FLTPLDD335 pKa = 3.9LRR337 pKa = 11.84AFDD340 pKa = 4.13VIGWDD345 pKa = 3.97LSVTDD350 pKa = 5.1PVPEE354 pKa = 4.18PTTLLLFGAGLSGLIYY370 pKa = 10.33VRR372 pKa = 11.84SRR374 pKa = 11.84KK375 pKa = 9.1KK376 pKa = 10.48RR377 pKa = 11.84KK378 pKa = 9.18

Molecular weight: 41.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1P2T4|M1P2T4_DESSD Uncharacterized protein OS=Desulfocapsa sulfexigens (strain DSM 10523 / SB164P1) OX=1167006 GN=UWK_01232 PE=4 SV=1
MM1 pKa = 7.59PPKK4 pKa = 10.37KK5 pKa = 9.98RR6 pKa = 11.84VFQRR10 pKa = 11.84KK11 pKa = 8.75RR12 pKa = 11.84SCRR15 pKa = 11.84FCSDD19 pKa = 2.86KK20 pKa = 11.1EE21 pKa = 3.78MSIDD25 pKa = 3.68YY26 pKa = 10.27KK27 pKa = 11.12DD28 pKa = 3.61PKK30 pKa = 8.85TLRR33 pKa = 11.84NFVTEE38 pKa = 4.04RR39 pKa = 11.84GKK41 pKa = 10.51IIPRR45 pKa = 11.84RR46 pKa = 11.84IYY48 pKa = 8.18GTCATHH54 pKa = 6.63QRR56 pKa = 11.84QLCEE60 pKa = 3.94AIKK63 pKa = 10.25RR64 pKa = 11.84ARR66 pKa = 11.84QIALLPYY73 pKa = 10.19SGSTQRR79 pKa = 4.61

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3514

0

3514

1190136

23

2577

338.7

37.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.674 ± 0.037

1.329 ± 0.015

5.493 ± 0.041

6.508 ± 0.036

4.471 ± 0.027

7.155 ± 0.04

2.172 ± 0.019

7.018 ± 0.029

5.719 ± 0.04

10.483 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.018

3.946 ± 0.025

4.075 ± 0.025

3.685 ± 0.028

5.006 ± 0.033

6.571 ± 0.036

5.461 ± 0.026

6.689 ± 0.033

1.018 ± 0.013

2.932 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski