Clostridium intestinale URNW

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium intestinale

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U2NM21|U2NM21_9CLOT ABC transporter OS=Clostridium intestinale URNW OX=1294142 GN=CINTURNW_2905 PE=4 SV=1
MM1 pKa = 7.7LLFKK5 pKa = 10.34IDD7 pKa = 3.28GVSFYY12 pKa = 11.17KK13 pKa = 10.61EE14 pKa = 4.23DD15 pKa = 3.89FQDD18 pKa = 5.47DD19 pKa = 4.14FYY21 pKa = 10.86EE22 pKa = 4.36YY23 pKa = 10.85QDD25 pKa = 4.06IIPIIEE31 pKa = 4.21EE32 pKa = 3.84FQDD35 pKa = 3.29ALNIEE40 pKa = 5.09KK41 pKa = 8.59ITCADD46 pKa = 4.13LNDD49 pKa = 4.16CCEE52 pKa = 4.15QTKK55 pKa = 10.54EE56 pKa = 4.04NYY58 pKa = 8.53FAQIQGFINEE68 pKa = 4.11EE69 pKa = 4.16DD70 pKa = 3.6EE71 pKa = 4.68FLLIDD76 pKa = 3.65EE77 pKa = 4.44VEE79 pKa = 4.17EE80 pKa = 4.62GKK82 pKa = 9.87EE83 pKa = 3.82HH84 pKa = 7.7LDD86 pKa = 3.35LFVIRR91 pKa = 11.84IYY93 pKa = 11.2KK94 pKa = 10.37CMSCNKK100 pKa = 8.99WMIDD104 pKa = 3.02ILEE107 pKa = 4.13

Molecular weight: 12.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2NRU4|U2NRU4_9CLOT Catalase OS=Clostridium intestinale URNW OX=1294142 GN=CINTURNW_0809 PE=3 SV=1
MM1 pKa = 7.34IQTNHH6 pKa = 3.75NTIRR10 pKa = 11.84QGYY13 pKa = 4.37TRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TRR19 pKa = 11.84LISVNIILIVLTLLLCGIMLVYY41 pKa = 10.38GKK43 pKa = 10.16NIYY46 pKa = 10.05DD47 pKa = 4.27LNTVIRR53 pKa = 11.84VLSGEE58 pKa = 4.23QIQGATFTIGTLRR71 pKa = 11.84LPRR74 pKa = 11.84MLCGLLAGLAFGIAGNTFQTMLRR97 pKa = 11.84NPLASPDD104 pKa = 3.6IIGISSGSSVAAVFCILVLRR124 pKa = 11.84ISGTGVSIAAVVSGILVASLIYY146 pKa = 10.4VLSKK150 pKa = 11.11GSGFSGGRR158 pKa = 11.84LILIGIGMQAMINALISYY176 pKa = 8.99LLAKK180 pKa = 10.31SSQHH184 pKa = 6.49DD185 pKa = 3.89VPTALRR191 pKa = 11.84WLNGSLNGMVIEE203 pKa = 4.69DD204 pKa = 3.81VPFLFVVVVVFGITILCLTKK224 pKa = 10.23QLQVLEE230 pKa = 5.02LGDD233 pKa = 4.09EE234 pKa = 4.38FATTLGVRR242 pKa = 11.84INVVRR247 pKa = 11.84IMLILSAVFLIAFSTAVTGPISFVAFLAGPIAARR281 pKa = 11.84LVGLGSPNVLSAGLVGALLVLSADD305 pKa = 5.12LIGQFAFGTRR315 pKa = 11.84FPVGVITGILGAPYY329 pKa = 9.75MLFLLIVINRR339 pKa = 11.84RR340 pKa = 11.84SQII343 pKa = 3.33

Molecular weight: 36.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4340

0

4340

1276823

35

2582

294.2

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.571 ± 0.041

0.993 ± 0.016

5.488 ± 0.028

7.633 ± 0.046

4.402 ± 0.029

6.523 ± 0.045

1.336 ± 0.015

9.909 ± 0.051

8.899 ± 0.035

9.329 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.015

6.099 ± 0.035

2.777 ± 0.025

2.227 ± 0.02

3.332 ± 0.024

6.587 ± 0.034

4.982 ± 0.041

6.57 ± 0.033

0.7 ± 0.011

4.11 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski