Amino acid dipepetide frequency for Clostridium intestinale URNW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.697AlaAla: 3.697 ± 0.068	0.617AlaCys: 0.617 ± 0.024	2.543AlaAsp: 2.543 ± 0.043	3.409AlaGlu: 3.409 ± 0.056	2.534AlaPhe: 2.534 ± 0.053	3.708AlaGly: 3.708 ± 0.072	0.828AlaHis: 0.828 ± 0.028	5.724AlaIle: 5.724 ± 0.075	4.501AlaLys: 4.501 ± 0.061	6.157AlaLeu: 6.157 ± 0.083	1.612AlaMet: 1.612 ± 0.04	2.543AlaAsn: 2.543 ± 0.052	1.479AlaPro: 1.479 ± 0.056	1.389AlaGln: 1.389 ± 0.034	1.841AlaArg: 1.841 ± 0.041	3.452AlaSer: 3.452 ± 0.07	3.098AlaThr: 3.098 ± 0.096	4.03AlaVal: 4.03 ± 0.065	0.433AlaTrp: 0.433 ± 0.019	2.115AlaTyr: 2.115 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.519CysAla: 0.519 ± 0.021	0.163CysCys: 0.163 ± 0.012	0.576CysAsp: 0.576 ± 0.022	0.704CysGlu: 0.704 ± 0.026	0.436CysPhe: 0.436 ± 0.021	0.895CysGly: 0.895 ± 0.029	0.179CysHis: 0.179 ± 0.011	0.94CysIle: 0.94 ± 0.03	0.765CysLys: 0.765 ± 0.028	0.8CysLeu: 0.8 ± 0.029	0.244CysMet: 0.244 ± 0.014	0.57CysAsn: 0.57 ± 0.021	0.422CysPro: 0.422 ± 0.02	0.168CysGln: 0.168 ± 0.011	0.335CysArg: 0.335 ± 0.017	0.692CysSer: 0.692 ± 0.023	0.508CysThr: 0.508 ± 0.021	0.582CysVal: 0.582 ± 0.022	0.057CysTrp: 0.057 ± 0.008	0.377CysTyr: 0.377 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	2.487AspAla: 2.487 ± 0.043	0.468AspCys: 0.468 ± 0.021	2.548AspAsp: 2.548 ± 0.042	4.699AspGlu: 4.699 ± 0.059	2.832AspPhe: 2.832 ± 0.048	3.536AspGly: 3.536 ± 0.068	0.544AspHis: 0.544 ± 0.025	6.208AspIle: 6.208 ± 0.072	5.556AspLys: 5.556 ± 0.066	4.987AspLeu: 4.987 ± 0.066	1.5AspMet: 1.5 ± 0.037	3.291AspAsn: 3.291 ± 0.061	1.299AspPro: 1.299 ± 0.033	0.764AspGln: 0.764 ± 0.025	1.889AspArg: 1.889 ± 0.039	3.346AspSer: 3.346 ± 0.057	2.411AspThr: 2.411 ± 0.049	3.54AspVal: 3.54 ± 0.053	0.386AspTrp: 0.386 ± 0.022	2.588AspTyr: 2.588 ± 0.053	0.0AspXaa: 0.0 ± 0.0
Glu	4.581GluAla: 4.581 ± 0.065	0.613GluCys: 0.613 ± 0.025	4.671GluAsp: 4.671 ± 0.064	7.344GluGlu: 7.344 ± 0.094	3.076GluPhe: 3.076 ± 0.051	4.39GluGly: 4.39 ± 0.059	0.952GluHis: 0.952 ± 0.025	7.348GluIle: 7.348 ± 0.086	7.57GluLys: 7.57 ± 0.087	7.172GluLeu: 7.172 ± 0.085	1.875GluMet: 1.875 ± 0.038	5.111GluAsn: 5.111 ± 0.074	1.473GluPro: 1.473 ± 0.036	1.729GluGln: 1.729 ± 0.041	2.59GluArg: 2.59 ± 0.05	3.775GluSer: 3.775 ± 0.055	3.123GluThr: 3.123 ± 0.052	5.304GluVal: 5.304 ± 0.064	0.477GluTrp: 0.477 ± 0.016	3.157GluTyr: 3.157 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	1.938PheAla: 1.938 ± 0.04	0.47PheCys: 0.47 ± 0.021	2.426PheAsp: 2.426 ± 0.042	2.714PheGlu: 2.714 ± 0.049	1.931PhePhe: 1.931 ± 0.047	2.881PheGly: 2.881 ± 0.054	0.536PheHis: 0.536 ± 0.021	4.821PheIle: 4.821 ± 0.073	3.892PheLys: 3.892 ± 0.067	4.184PheLeu: 4.184 ± 0.065	1.207PheMet: 1.207 ± 0.03	2.959PheAsn: 2.959 ± 0.051	1.197PhePro: 1.197 ± 0.03	0.963PheGln: 0.963 ± 0.027	1.306PheArg: 1.306 ± 0.036	3.464PheSer: 3.464 ± 0.059	2.365PheThr: 2.365 ± 0.041	2.596PheVal: 2.596 ± 0.043	0.314PheTrp: 0.314 ± 0.016	1.859PheTyr: 1.859 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	4.458GlyAla: 4.458 ± 0.103	0.765GlyCys: 0.765 ± 0.025	3.422GlyAsp: 3.422 ± 0.058	4.611GlyGlu: 4.611 ± 0.065	3.181GlyPhe: 3.181 ± 0.05	4.402GlyGly: 4.402 ± 0.09	0.939GlyHis: 0.939 ± 0.028	6.728GlyIle: 6.728 ± 0.078	5.243GlyLys: 5.243 ± 0.068	5.543GlyLeu: 5.543 ± 0.075	1.706GlyMet: 1.706 ± 0.038	3.314GlyAsn: 3.314 ± 0.056	1.237GlyPro: 1.237 ± 0.042	1.483GlyGln: 1.483 ± 0.037	2.125GlyArg: 2.125 ± 0.046	3.856GlySer: 3.856 ± 0.062	3.506GlyThr: 3.506 ± 0.06	5.182GlyVal: 5.182 ± 0.077	0.524GlyTrp: 0.524 ± 0.022	3.006GlyTyr: 3.006 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	0.659HisAla: 0.659 ± 0.022	0.19HisCys: 0.19 ± 0.012	0.693HisAsp: 0.693 ± 0.025	0.902HisGlu: 0.902 ± 0.029	0.636HisPhe: 0.636 ± 0.024	1.01HisGly: 1.01 ± 0.031	0.3HisHis: 0.3 ± 0.017	1.289HisIle: 1.289 ± 0.032	1.067HisLys: 1.067 ± 0.028	1.204HisLeu: 1.204 ± 0.032	0.331HisMet: 0.331 ± 0.016	0.768HisAsn: 0.768 ± 0.025	0.566HisPro: 0.566 ± 0.023	0.333HisGln: 0.333 ± 0.015	0.511HisArg: 0.511 ± 0.02	0.85HisSer: 0.85 ± 0.027	0.62HisThr: 0.62 ± 0.021	0.757HisVal: 0.757 ± 0.025	0.114HisTrp: 0.114 ± 0.01	0.556HisTyr: 0.556 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	5.722IleAla: 5.722 ± 0.073	1.065IleCys: 1.065 ± 0.028	5.732IleAsp: 5.732 ± 0.081	7.297IleGlu: 7.297 ± 0.07	4.244IlePhe: 4.244 ± 0.066	6.071IleGly: 6.071 ± 0.074	1.284IleHis: 1.284 ± 0.03	9.225IleIle: 9.225 ± 0.116	8.85IleLys: 8.85 ± 0.105	9.34IleLeu: 9.34 ± 0.118	2.386IleMet: 2.386 ± 0.046	6.525IleAsn: 6.525 ± 0.087	3.246IlePro: 3.246 ± 0.047	2.155IleGln: 2.155 ± 0.042	3.13IleArg: 3.13 ± 0.054	7.325IleSer: 7.325 ± 0.099	5.171IleThr: 5.171 ± 0.076	6.081IleVal: 6.081 ± 0.075	0.538IleTrp: 0.538 ± 0.023	3.705IleTyr: 3.705 ± 0.06	0.0IleXaa: 0.0 ± 0.0
Lys	5.121LysAla: 5.121 ± 0.082	0.687LysCys: 0.687 ± 0.028	6.083LysAsp: 6.083 ± 0.077	9.082LysGlu: 9.082 ± 0.099	3.084LysPhe: 3.084 ± 0.05	5.182LysGly: 5.182 ± 0.068	1.054LysHis: 1.054 ± 0.031	8.151LysIle: 8.151 ± 0.093	7.704LysLys: 7.704 ± 0.089	7.743LysLeu: 7.743 ± 0.084	2.207LysMet: 2.207 ± 0.04	5.942LysAsn: 5.942 ± 0.078	1.996LysPro: 1.996 ± 0.04	2.04LysGln: 2.04 ± 0.041	3.037LysArg: 3.037 ± 0.05	5.182LysSer: 5.182 ± 0.073	4.091LysThr: 4.091 ± 0.061	6.114LysVal: 6.114 ± 0.065	0.595LysTrp: 0.595 ± 0.019	3.891LysTyr: 3.891 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	5.351LeuAla: 5.351 ± 0.071	0.988LeuCys: 0.988 ± 0.025	5.305LeuAsp: 5.305 ± 0.072	6.833LeuGlu: 6.833 ± 0.079	3.892LeuPhe: 3.892 ± 0.063	6.374LeuGly: 6.374 ± 0.079	1.099LeuHis: 1.099 ± 0.03	8.827LeuIle: 8.827 ± 0.11	8.682LeuLys: 8.682 ± 0.082	8.338LeuLeu: 8.338 ± 0.109	2.413LeuMet: 2.413 ± 0.041	5.962LeuAsn: 5.962 ± 0.082	2.773LeuPro: 2.773 ± 0.05	2.019LeuGln: 2.019 ± 0.043	3.114LeuArg: 3.114 ± 0.06	7.006LeuSer: 7.006 ± 0.08	4.72LeuThr: 4.72 ± 0.065	5.728LeuVal: 5.728 ± 0.075	0.615LeuTrp: 0.615 ± 0.021	3.254LeuTyr: 3.254 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	1.547MetAla: 1.547 ± 0.04	0.202MetCys: 0.202 ± 0.014	1.493MetAsp: 1.493 ± 0.036	1.8MetGlu: 1.8 ± 0.038	1.055MetPhe: 1.055 ± 0.032	1.783MetGly: 1.783 ± 0.044	0.381MetHis: 0.381 ± 0.017	2.35MetIle: 2.35 ± 0.045	2.509MetLys: 2.509 ± 0.042	2.164MetLeu: 2.164 ± 0.046	0.648MetMet: 0.648 ± 0.023	1.599MetAsn: 1.599 ± 0.036	0.835MetPro: 0.835 ± 0.025	0.648MetGln: 0.648 ± 0.025	0.881MetArg: 0.881 ± 0.025	1.678MetSer: 1.678 ± 0.037	1.1MetThr: 1.1 ± 0.031	1.632MetVal: 1.632 ± 0.032	0.165MetTrp: 0.165 ± 0.011	0.843MetTyr: 0.843 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.625AsnAla: 2.625 ± 0.046	0.576AsnCys: 0.576 ± 0.025	2.802AsnAsp: 2.802 ± 0.05	4.398AsnGlu: 4.398 ± 0.065	2.59AsnPhe: 2.59 ± 0.045	3.657AsnGly: 3.657 ± 0.069	0.789AsnHis: 0.789 ± 0.026	7.003AsnIle: 7.003 ± 0.085	6.01AsnLys: 6.01 ± 0.081	5.785AsnLeu: 5.785 ± 0.075	1.519AsnMet: 1.519 ± 0.035	4.134AsnAsn: 4.134 ± 0.069	2.172AsnPro: 2.172 ± 0.045	1.292AsnGln: 1.292 ± 0.032	2.004AsnArg: 2.004 ± 0.044	4.228AsnSer: 4.228 ± 0.065	2.899AsnThr: 2.899 ± 0.055	3.408AsnVal: 3.408 ± 0.059	0.444AsnTrp: 0.444 ± 0.018	2.657AsnTyr: 2.657 ± 0.047	0.0AsnXaa: 0.0 ± 0.0
Pro	1.381ProAla: 1.381 ± 0.032	0.269ProCys: 0.269 ± 0.016	1.433ProAsp: 1.433 ± 0.037	2.217ProGlu: 2.217 ± 0.042	1.425ProPhe: 1.425 ± 0.032	1.625ProGly: 1.625 ± 0.036	0.448ProHis: 0.448 ± 0.019	2.677ProIle: 2.677 ± 0.047	2.279ProLys: 2.279 ± 0.048	2.635ProLeu: 2.635 ± 0.047	0.688ProMet: 0.688 ± 0.022	1.62ProAsn: 1.62 ± 0.033	0.611ProPro: 0.611 ± 0.027	0.749ProGln: 0.749 ± 0.025	0.845ProArg: 0.845 ± 0.025	1.731ProSer: 1.731 ± 0.042	1.499ProThr: 1.499 ± 0.046	2.19ProVal: 2.19 ± 0.111	0.237ProTrp: 0.237 ± 0.015	1.215ProTyr: 1.215 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.358GlnAla: 1.358 ± 0.031	0.2GlnCys: 0.2 ± 0.012	1.161GlnAsp: 1.161 ± 0.031	1.812GlnGlu: 1.812 ± 0.037	0.808GlnPhe: 0.808 ± 0.022	1.661GlnGly: 1.661 ± 0.039	0.271GlnHis: 0.271 ± 0.014	2.036GlnIle: 2.036 ± 0.042	1.958GlnLys: 1.958 ± 0.035	2.017GlnLeu: 2.017 ± 0.049	0.552GlnMet: 0.552 ± 0.021	1.367GlnAsn: 1.367 ± 0.031	0.516GlnPro: 0.516 ± 0.02	0.614GlnGln: 0.614 ± 0.028	0.862GlnArg: 0.862 ± 0.024	1.328GlnSer: 1.328 ± 0.034	1.031GlnThr: 1.031 ± 0.034	1.541GlnVal: 1.541 ± 0.034	0.205GlnTrp: 0.205 ± 0.012	0.971GlnTyr: 0.971 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	1.872ArgAla: 1.872 ± 0.036	0.352ArgCys: 0.352 ± 0.02	1.795ArgAsp: 1.795 ± 0.043	2.986ArgGlu: 2.986 ± 0.057	1.507ArgPhe: 1.507 ± 0.036	2.038ArgGly: 2.038 ± 0.043	0.446ArgHis: 0.446 ± 0.021	3.105ArgIle: 3.105 ± 0.059	3.028ArgLys: 3.028 ± 0.055	2.98ArgLeu: 2.98 ± 0.052	0.89ArgMet: 0.89 ± 0.027	2.014ArgAsn: 2.014 ± 0.044	0.766ArgPro: 0.766 ± 0.027	0.753ArgGln: 0.753 ± 0.029	1.382ArgArg: 1.382 ± 0.04	1.729ArgSer: 1.729 ± 0.036	1.53ArgThr: 1.53 ± 0.035	2.36ArgVal: 2.36 ± 0.046	0.259ArgTrp: 0.259 ± 0.015	1.529ArgTyr: 1.529 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.153SerAla: 3.153 ± 0.056	0.604SerCys: 0.604 ± 0.021	3.214SerAsp: 3.214 ± 0.055	4.442SerGlu: 4.442 ± 0.058	3.241SerPhe: 3.241 ± 0.055	4.355SerGly: 4.355 ± 0.064	0.937SerHis: 0.937 ± 0.027	6.866SerIle: 6.866 ± 0.089	5.987SerLys: 5.987 ± 0.071	6.672SerLeu: 6.672 ± 0.089	1.664SerMet: 1.664 ± 0.039	3.843SerAsn: 3.843 ± 0.059	1.792SerPro: 1.792 ± 0.05	1.591SerGln: 1.591 ± 0.038	2.152SerArg: 2.152 ± 0.042	4.777SerSer: 4.777 ± 0.088	3.402SerThr: 3.402 ± 0.061	3.928SerVal: 3.928 ± 0.066	0.446SerTrp: 0.446 ± 0.019	2.802SerTyr: 2.802 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	2.889ThrAla: 2.889 ± 0.061	0.432ThrCys: 0.432 ± 0.016	2.343ThrAsp: 2.343 ± 0.049	3.089ThrGlu: 3.089 ± 0.052	2.243ThrPhe: 2.243 ± 0.047	4.068ThrGly: 4.068 ± 0.157	0.773ThrHis: 0.773 ± 0.025	4.784ThrIle: 4.784 ± 0.07	3.795ThrLys: 3.795 ± 0.057	5.189ThrLeu: 5.189 ± 0.064	1.103ThrMet: 1.103 ± 0.031	2.69ThrAsn: 2.69 ± 0.054	1.994ThrPro: 1.994 ± 0.059	1.184ThrGln: 1.184 ± 0.026	1.485ThrArg: 1.485 ± 0.038	3.521ThrSer: 3.521 ± 0.059	2.682ThrThr: 2.682 ± 0.064	3.245ThrVal: 3.245 ± 0.057	0.396ThrTrp: 0.396 ± 0.019	1.913ThrTyr: 1.913 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.044ValAla: 4.044 ± 0.085	0.734ValCys: 0.734 ± 0.026	3.888ValAsp: 3.888 ± 0.057	4.593ValGlu: 4.593 ± 0.058	3.0ValPhe: 3.0 ± 0.051	4.301ValGly: 4.301 ± 0.072	0.901ValHis: 0.901 ± 0.025	6.378ValIle: 6.378 ± 0.071	5.294ValLys: 5.294 ± 0.056	6.007ValLeu: 6.007 ± 0.078	1.602ValMet: 1.602 ± 0.031	3.668ValAsn: 3.668 ± 0.056	2.001ValPro: 2.001 ± 0.04	1.428ValGln: 1.428 ± 0.038	2.038ValArg: 2.038 ± 0.045	4.564ValSer: 4.564 ± 0.065	3.614ValThr: 3.614 ± 0.069	4.732ValVal: 4.732 ± 0.078	0.399ValTrp: 0.399 ± 0.016	2.515ValTyr: 2.515 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.403TrpAla: 0.403 ± 0.018	0.085TrpCys: 0.085 ± 0.008	0.389TrpAsp: 0.389 ± 0.017	0.454TrpGlu: 0.454 ± 0.018	0.319TrpPhe: 0.319 ± 0.017	0.571TrpGly: 0.571 ± 0.022	0.117TrpHis: 0.117 ± 0.01	0.656TrpIle: 0.656 ± 0.025	0.537TrpLys: 0.537 ± 0.024	0.615TrpLeu: 0.615 ± 0.022	0.198TrpMet: 0.198 ± 0.013	0.453TrpAsn: 0.453 ± 0.02	0.143TrpPro: 0.143 ± 0.012	0.199TrpGln: 0.199 ± 0.013	0.298TrpArg: 0.298 ± 0.015	0.451TrpSer: 0.451 ± 0.019	0.335TrpThr: 0.335 ± 0.018	0.438TrpVal: 0.438 ± 0.02	0.081TrpTrp: 0.081 ± 0.009	0.262TrpTyr: 0.262 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.906TyrAla: 1.906 ± 0.041	0.454TyrCys: 0.454 ± 0.019	2.362TyrAsp: 2.362 ± 0.046	2.942TyrGlu: 2.942 ± 0.057	1.986TyrPhe: 1.986 ± 0.039	2.725TyrGly: 2.725 ± 0.048	0.578TyrHis: 0.578 ± 0.02	3.977TyrIle: 3.977 ± 0.069	3.762TyrLys: 3.762 ± 0.054	3.757TyrLeu: 3.757 ± 0.057	0.967TyrMet: 0.967 ± 0.026	2.621TyrAsn: 2.621 ± 0.05	1.257TyrPro: 1.257 ± 0.032	0.767TyrGln: 0.767 ± 0.027	1.493TyrArg: 1.493 ± 0.034	2.915TyrSer: 2.915 ± 0.047	2.111TyrThr: 2.111 ± 0.048	2.317TyrVal: 2.317 ± 0.04	0.317TyrTrp: 0.317 ± 0.016	1.889TyrTyr: 1.889 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4340 proteins (1276823 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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