Erinaceus europaeus (Western European hedgehog)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Eulipotyphl

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S3WIK7|A0A1S3WIK7_ERIEU CD160 antigen OS=Erinaceus europaeus OX=9365 GN=CD160 PE=4 SV=1
MM1 pKa = 7.35EE2 pKa = 6.23ASTLTPSEE10 pKa = 4.6LGLLLLPVLAVLLLALCARR29 pKa = 11.84CRR31 pKa = 11.84DD32 pKa = 3.72LPSSYY37 pKa = 10.59EE38 pKa = 4.18SNDD41 pKa = 3.45SYY43 pKa = 10.84THH45 pKa = 6.7SSGLVIKK52 pKa = 10.37SPSTMAPWIPDD63 pKa = 3.02SSYY66 pKa = 11.16PPMVCCPPLSQPDD79 pKa = 4.07LLPISRR85 pKa = 11.84SPRR88 pKa = 11.84LSGGSHH94 pKa = 6.57RR95 pKa = 11.84MQPPRR100 pKa = 11.84QDD102 pKa = 3.09SASANSVASYY112 pKa = 10.36EE113 pKa = 4.19NQEE116 pKa = 4.17AACEE120 pKa = 4.2DD121 pKa = 4.01EE122 pKa = 6.1DD123 pKa = 5.14EE124 pKa = 5.46EE125 pKa = 6.41DD126 pKa = 5.85DD127 pKa = 3.75EE128 pKa = 4.8DD129 pKa = 4.53HH130 pKa = 7.14NEE132 pKa = 4.34GYY134 pKa = 10.97LEE136 pKa = 4.03VLPDD140 pKa = 3.81SVPATGTSAPPVPRR154 pKa = 11.84PNNPSFRR161 pKa = 11.84DD162 pKa = 3.37SAFSMEE168 pKa = 4.23SGEE171 pKa = 4.99DD172 pKa = 3.63YY173 pKa = 11.66VNVPEE178 pKa = 4.68SEE180 pKa = 4.29EE181 pKa = 4.1SADD184 pKa = 3.58ASLDD188 pKa = 3.44GSRR191 pKa = 11.84EE192 pKa = 3.99YY193 pKa = 11.72VNVSQEE199 pKa = 4.14LPPPPEE205 pKa = 4.31TEE207 pKa = 3.75PATPSSQEE215 pKa = 3.75VEE217 pKa = 4.35EE218 pKa = 4.67EE219 pKa = 3.94EE220 pKa = 4.8GAPDD224 pKa = 3.71YY225 pKa = 11.74EE226 pKa = 4.53NVQEE230 pKa = 4.0LHH232 pKa = 6.33

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S3WUG8|A0A1S3WUG8_ERIEU death effector domain-containing protein isoform X1 OS=Erinaceus europaeus OX=9365 GN=DEDD PE=4 SV=1
MM1 pKa = 7.57SSHH4 pKa = 5.15KK5 pKa = 8.91TFRR8 pKa = 11.84IKK10 pKa = 10.64RR11 pKa = 11.84FLAKK15 pKa = 9.71KK16 pKa = 9.58QKK18 pKa = 8.69QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.3RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 3.95WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19244

8384

27628

16097671

28

33415

582.7

64.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.014

2.178 ± 0.012

4.836 ± 0.011

6.974 ± 0.019

3.666 ± 0.011

6.503 ± 0.021

2.591 ± 0.007

4.338 ± 0.013

5.595 ± 0.018

10.061 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.196 ± 0.006

3.563 ± 0.01

6.334 ± 0.023

4.789 ± 0.015

5.7 ± 0.014

8.371 ± 0.018

5.365 ± 0.016

6.065 ± 0.013

1.181 ± 0.005

2.677 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski