Mytilus galloprovincialis (Mediterranean mussel)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Bivalvia; Autobranchia; Pteriomorphia; Mytiloida; Mytiloidea; Mytilidae; Mytilinae; Mytilus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3147 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3L5TR72|A0A3L5TR72_MYTGA Sodium-transporter and chloride-dependent neurotransmitter (Fragment) OS=Mytilus galloprovincialis OX=29158 GN=AM593_02716 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.33RR3 pKa = 11.84KK4 pKa = 9.28ILHH7 pKa = 6.51RR8 pKa = 11.84KK9 pKa = 9.23LKK11 pKa = 9.77TEE13 pKa = 3.87QHH15 pKa = 5.1EE16 pKa = 4.46HH17 pKa = 4.62NQKK20 pKa = 10.66LGLISMTFEE29 pKa = 4.33GQCKK33 pKa = 9.21FGRR36 pKa = 11.84RR37 pKa = 11.84NYY39 pKa = 9.8VLKK42 pKa = 10.48QASQLGVARR51 pKa = 11.84QLDD54 pKa = 4.06TMVTLCRR61 pKa = 11.84FQPDD65 pKa = 3.12VTIQIDD71 pKa = 4.11RR72 pKa = 11.84LPALAMVYY80 pKa = 10.21CRR82 pKa = 11.84TEE84 pKa = 3.91DD85 pKa = 3.74VITVAQSSSTIDD97 pKa = 4.36PITEE101 pKa = 4.7IITVPPIIPIILDD114 pKa = 3.59DD115 pKa = 4.1VLLSGALVRR124 pKa = 11.84DD125 pKa = 3.76LLYY128 pKa = 11.16SNKK131 pKa = 9.64KK132 pKa = 9.49DD133 pKa = 3.19INVIISKK140 pKa = 10.09EE141 pKa = 3.92ILSASLDD148 pKa = 3.59VDD150 pKa = 4.17DD151 pKa = 4.34VCPRR155 pKa = 11.84SVDD158 pKa = 3.66DD159 pKa = 3.82VCVKK163 pKa = 10.5FVDD166 pKa = 4.22AVCAGFVDD174 pKa = 4.87DD175 pKa = 4.04VCVGYY180 pKa = 10.38FEE182 pKa = 5.06VVCTRR187 pKa = 11.84FVDD190 pKa = 4.61DD191 pKa = 3.43VCKK194 pKa = 10.57RR195 pKa = 11.84LLNDD199 pKa = 2.88VCTVFVDD206 pKa = 4.39DD207 pKa = 3.9VCAGFVDD214 pKa = 5.89DD215 pKa = 3.81VCAWFVDD222 pKa = 4.02DD223 pKa = 5.05VFAGFIANVCAEE235 pKa = 4.4FVDD238 pKa = 4.25DD239 pKa = 4.62LCAGFVDD246 pKa = 4.87DD247 pKa = 4.19VCAGFVDD254 pKa = 4.73DD255 pKa = 4.14VCAGFVDD262 pKa = 4.73DD263 pKa = 3.97VCAGFVVDD271 pKa = 3.82VCAGFVDD278 pKa = 4.73DD279 pKa = 4.14VCAGFVDD286 pKa = 4.51KK287 pKa = 11.1VWAGFVDD294 pKa = 5.98DD295 pKa = 3.89VCTWFDD301 pKa = 3.66DD302 pKa = 4.39FVFAGFVDD310 pKa = 4.65DD311 pKa = 3.97VCAGFVDD318 pKa = 4.73DD319 pKa = 4.14VCAGFVDD326 pKa = 4.73DD327 pKa = 4.14VCAGFVDD334 pKa = 4.73DD335 pKa = 3.97VCAGFVVDD343 pKa = 3.82VCAGFVDD350 pKa = 4.73DD351 pKa = 4.14VCAGFVDD358 pKa = 4.51KK359 pKa = 11.1VWAGFVDD366 pKa = 4.87DD367 pKa = 5.35GLLMMYY373 pKa = 9.93VQGLLMMSAQGLLMISAHH391 pKa = 6.53ALLMMSAHH399 pKa = 7.05ALLMMSAHH407 pKa = 6.41GLLMISAQGLL417 pKa = 3.6

Molecular weight: 45.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3L5TT46|A0A3L5TT46_MYTGA Uncharacterized protein (Fragment) OS=Mytilus galloprovincialis OX=29158 GN=AM593_08727 PE=4 SV=1
MM1 pKa = 7.6AVPAKK6 pKa = 10.04IVLKK10 pKa = 9.72STTKK14 pKa = 10.0MSLNDD19 pKa = 3.68RR20 pKa = 11.84FSTIQATVRR29 pKa = 11.84QPAQQASVSNIRR41 pKa = 11.84AKK43 pKa = 9.82MAAQHH48 pKa = 5.74QATAANKK55 pKa = 9.21RR56 pKa = 11.84LAMQMANRR64 pKa = 11.84PSVQAALKK72 pKa = 10.09IKK74 pKa = 10.36KK75 pKa = 9.7KK76 pKa = 9.28SLKK79 pKa = 9.71QRR81 pKa = 11.84LGFGNTSVKK90 pKa = 10.5SRR92 pKa = 11.84LTLGGRR98 pKa = 11.84GGGAIVRR105 pKa = 11.84GGRR108 pKa = 11.84GQGRR112 pKa = 11.84LMRR115 pKa = 11.84GRR117 pKa = 11.84GQMRR121 pKa = 11.84GRR123 pKa = 11.84GRR125 pKa = 11.84GGRR128 pKa = 11.84GGSGSTPGSPTGNQMVRR145 pKa = 11.84GGNRR149 pKa = 11.84GQRR152 pKa = 11.84GGFVQRR158 pKa = 11.84GRR160 pKa = 11.84GQRR163 pKa = 11.84GGFVQRR169 pKa = 11.84GEE171 pKa = 3.81NRR173 pKa = 11.84GRR175 pKa = 11.84RR176 pKa = 11.84GGMQRR181 pKa = 11.84GRR183 pKa = 11.84GFQRR187 pKa = 11.84GNRR190 pKa = 11.84GGRR193 pKa = 11.84GQTQGFRR200 pKa = 11.84GNRR203 pKa = 11.84GSGGFRR209 pKa = 3.24

Molecular weight: 22.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3147

0

3147

761033

18

2702

241.8

27.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.877 ± 0.036

2.443 ± 0.043

6.143 ± 0.047

6.574 ± 0.054

3.878 ± 0.034

5.624 ± 0.069

2.417 ± 0.025

6.442 ± 0.047

7.278 ± 0.061

8.234 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.413 ± 0.021

5.551 ± 0.042

4.145 ± 0.048

4.186 ± 0.041

4.515 ± 0.038

7.787 ± 0.048

6.738 ± 0.092

6.227 ± 0.039

1.079 ± 0.017

3.445 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski