Apis mellifera associated microvirus 14

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTY1|A0A3S8UTY1_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 14 OX=2494741 PE=4 SV=1
MM1 pKa = 7.78DD2 pKa = 5.62AEE4 pKa = 4.2TGEE7 pKa = 4.3IIEE10 pKa = 4.7RR11 pKa = 11.84KK12 pKa = 9.41PPFIRR17 pKa = 11.84TAYY20 pKa = 8.45NYY22 pKa = 10.88SMEE25 pKa = 4.37AVSEE29 pKa = 4.3EE30 pKa = 4.23TGLTCPEE37 pKa = 4.23DD38 pKa = 3.58SRR40 pKa = 11.84TKK42 pKa = 10.63QEE44 pKa = 4.63FKK46 pKa = 11.24DD47 pKa = 3.49EE48 pKa = 3.98ADD50 pKa = 2.98INKK53 pKa = 9.29IVEE56 pKa = 4.25RR57 pKa = 11.84FGLTGEE63 pKa = 4.63LPQNLRR69 pKa = 11.84MPISGDD75 pKa = 3.29FTGLGDD81 pKa = 3.48YY82 pKa = 10.56HH83 pKa = 6.75EE84 pKa = 4.99AMNLVLQAQEE94 pKa = 4.72EE95 pKa = 4.46FDD97 pKa = 3.76KK98 pKa = 11.48LPAKK102 pKa = 10.16VRR104 pKa = 11.84EE105 pKa = 4.19RR106 pKa = 11.84FGNDD110 pKa = 2.32AGAFIAWAEE119 pKa = 4.09RR120 pKa = 11.84EE121 pKa = 4.31DD122 pKa = 3.86NLEE125 pKa = 3.88EE126 pKa = 4.38AKK128 pKa = 10.98LLGIARR134 pKa = 11.84EE135 pKa = 4.36EE136 pKa = 4.22KK137 pKa = 10.67AKK139 pKa = 9.81PQPIEE144 pKa = 3.56VRR146 pKa = 11.84LAKK149 pKa = 10.66EE150 pKa = 3.96EE151 pKa = 4.25TPPPAA156 pKa = 5.48

Molecular weight: 17.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8U605|A0A3Q8U605_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 14 OX=2494741 PE=4 SV=1
MM1 pKa = 7.71PCYY4 pKa = 10.38NPLKK8 pKa = 10.39AYY10 pKa = 10.33QLTNGEE16 pKa = 4.32IKK18 pKa = 10.37FHH20 pKa = 5.36EE21 pKa = 4.31TRR23 pKa = 11.84NVQRR27 pKa = 11.84EE28 pKa = 4.15LKK30 pKa = 9.99LPCGQCIGCRR40 pKa = 11.84LEE42 pKa = 5.77RR43 pKa = 11.84SRR45 pKa = 11.84QWAVRR50 pKa = 11.84CMHH53 pKa = 6.81EE54 pKa = 4.19ARR56 pKa = 11.84MYY58 pKa = 9.21EE59 pKa = 4.11QNCFITLTYY68 pKa = 10.62APEE71 pKa = 4.19HH72 pKa = 5.47VPKK75 pKa = 10.51RR76 pKa = 11.84GSLDD80 pKa = 3.15YY81 pKa = 11.42SVFQKK86 pKa = 10.52FMKK89 pKa = 10.35RR90 pKa = 11.84LRR92 pKa = 11.84KK93 pKa = 8.18HH94 pKa = 5.65TGPEE98 pKa = 3.23RR99 pKa = 11.84VRR101 pKa = 11.84FYY103 pKa = 10.48MCGEE107 pKa = 4.04YY108 pKa = 10.94GEE110 pKa = 4.82TEE112 pKa = 4.25HH113 pKa = 7.05RR114 pKa = 11.84PHH116 pKa = 6.28YY117 pKa = 9.47HH118 pKa = 6.2ACLFGYY124 pKa = 9.6DD125 pKa = 3.69WPDD128 pKa = 2.64KK129 pKa = 10.44TFFRR133 pKa = 11.84TTPTGEE139 pKa = 3.61KK140 pKa = 9.94LYY142 pKa = 10.73RR143 pKa = 11.84SEE145 pKa = 4.2TLEE148 pKa = 4.02KK149 pKa = 9.99LWPYY153 pKa = 11.16GFATTGTLTFEE164 pKa = 4.03SAAYY168 pKa = 8.37VARR171 pKa = 11.84YY172 pKa = 6.32CTKK175 pKa = 10.71KK176 pKa = 9.16ITGHH180 pKa = 5.53NAKK183 pKa = 10.15DD184 pKa = 3.05HH185 pKa = 6.23YY186 pKa = 10.54KK187 pKa = 10.3RR188 pKa = 11.84QDD190 pKa = 3.07AEE192 pKa = 4.3GEE194 pKa = 4.36YY195 pKa = 10.48EE196 pKa = 4.1LEE198 pKa = 4.26PEE200 pKa = 4.5FGHH203 pKa = 6.56MSLKK207 pKa = 10.43PGIGAAWLAKK217 pKa = 10.3YY218 pKa = 10.61GNTDD222 pKa = 2.77VWPFDD227 pKa = 3.7HH228 pKa = 7.12VITRR232 pKa = 11.84GMEE235 pKa = 4.17TKK237 pKa = 10.12PPKK240 pKa = 10.79YY241 pKa = 9.5YY242 pKa = 10.91DD243 pKa = 4.43KK244 pKa = 10.69ILKK247 pKa = 8.13RR248 pKa = 11.84TNPDD252 pKa = 2.52RR253 pKa = 11.84FAEE256 pKa = 4.17IQEE259 pKa = 4.26SRR261 pKa = 11.84EE262 pKa = 3.62VTAAQWAADD271 pKa = 3.7QTPDD275 pKa = 3.05RR276 pKa = 11.84LAAKK280 pKa = 8.83EE281 pKa = 3.95QVKK284 pKa = 9.93RR285 pKa = 11.84AQLRR289 pKa = 11.84QLKK292 pKa = 8.43RR293 pKa = 11.84TLL295 pKa = 3.34

Molecular weight: 34.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1364

81

534

272.8

30.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.63 ± 1.22

0.953 ± 0.581

4.692 ± 0.301

7.185 ± 2.177

4.106 ± 0.602

6.891 ± 0.703

2.126 ± 0.713

4.839 ± 0.584

4.545 ± 1.052

6.672 ± 0.23

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.372 ± 0.343

4.839 ± 0.935

5.352 ± 1.109

4.912 ± 0.738

7.185 ± 1.031

4.472 ± 0.869

7.698 ± 0.991

4.179 ± 0.67

1.393 ± 0.226

3.959 ± 1.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski