Vicingus serpentipes
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2518 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C6RWW8|A0A5C6RWW8_9FLAO CoA transferase OS=Vicingus serpentipes OX=1926625 GN=FRY74_00430 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.27 SYY4 pKa = 10.89 LKK6 pKa = 10.57 QIFLFATLIVTNSALAQQLDD26 pKa = 3.75 WLGHH30 pKa = 4.95 FRR32 pKa = 11.84 STNHH36 pKa = 5.44 VKK38 pKa = 9.04 STAISTDD45 pKa = 2.85 NDD47 pKa = 3.47 GNIYY51 pKa = 10.35 VVGTIEE57 pKa = 5.37 IDD59 pKa = 3.84 SADD62 pKa = 4.28 LNPDD66 pKa = 2.76 AGTYY70 pKa = 9.39 YY71 pKa = 10.96 LSPEE75 pKa = 4.11 FSNAAFVCKK84 pKa = 10.1 LDD86 pKa = 3.79 SLGNFIWAKK95 pKa = 10.06 SFGGGSSISPTSVAADD111 pKa = 3.64 DD112 pKa = 4.1 FGGVYY117 pKa = 10.04 IGGSYY122 pKa = 10.6 FNTVDD127 pKa = 4.42 FDD129 pKa = 4.45 PGLGVYY135 pKa = 10.43 DD136 pKa = 3.38 LTVFGGSDD144 pKa = 3.49 LFLSKK149 pKa = 11.13 LNVNGDD155 pKa = 3.76 FEE157 pKa = 4.64 WAFNAGSTSTDD168 pKa = 2.93 AVTDD172 pKa = 3.53 IAVDD176 pKa = 3.26 IDD178 pKa = 3.81 NNVYY182 pKa = 8.05 ITGYY186 pKa = 10.42 FDD188 pKa = 4.45 YY189 pKa = 11.54 ADD191 pKa = 4.55 FDD193 pKa = 4.52 PGAGWFALNPPEE205 pKa = 4.04 KK206 pKa = 10.08 AGYY209 pKa = 7.58 IAKK212 pKa = 9.79 YY213 pKa = 9.92 KK214 pKa = 10.17 PDD216 pKa = 3.41 GSFVNAVDD224 pKa = 4.34 FEE226 pKa = 5.49 GDD228 pKa = 3.58 GPSGSNSIAIAPNGSLYY245 pKa = 11.0 VVGAFNSTIDD255 pKa = 3.62 FDD257 pKa = 4.58 PGVGTFNLTSISSNVDD273 pKa = 2.71 IYY275 pKa = 11.38 VCKK278 pKa = 10.48 LDD280 pKa = 4.04 TSLTFQWAKK289 pKa = 10.96 SFGSSSADD297 pKa = 3.17 NGHH300 pKa = 5.84 TVSVSDD306 pKa = 3.61 SDD308 pKa = 3.92 EE309 pKa = 4.0 PVITGGFWNTIDD321 pKa = 4.04 VDD323 pKa = 4.37 PGPSTYY329 pKa = 11.1 NVTSNGLVDD338 pKa = 4.62 NLLLKK343 pKa = 10.66 LDD345 pKa = 3.8 ASGNFMWANTYY356 pKa = 11.06 GNAQTEE362 pKa = 4.39 YY363 pKa = 11.03 SLDD366 pKa = 3.26 AAIAVNDD373 pKa = 4.91 DD374 pKa = 3.34 IYY376 pKa = 11.09 ILGKK380 pKa = 10.43 FIDD383 pKa = 4.84 SIDD386 pKa = 3.56 VDD388 pKa = 4.31 GGPGTQLIASVSPSDD403 pKa = 4.03 SYY405 pKa = 11.09 IARR408 pKa = 11.84 LDD410 pKa = 3.45 GSGNLISSGQLGGPGSVSGKK430 pKa = 9.28 GLCISNTQKK439 pKa = 10.32 IYY441 pKa = 9.14 HH442 pKa = 6.17 TGIFSGSAEE451 pKa = 4.62 LDD453 pKa = 3.3 PSAGIDD459 pKa = 3.51 SLTSLGLNDD468 pKa = 4.68 SYY470 pKa = 11.4 IQIAIPSAMVGLKK483 pKa = 9.54 TISTEE488 pKa = 4.12 TTKK491 pKa = 10.95 KK492 pKa = 10.35 EE493 pKa = 3.97 LIKK496 pKa = 10.65 IIDD499 pKa = 3.14 IMGRR503 pKa = 11.84 YY504 pKa = 9.26 IKK506 pKa = 10.07 DD507 pKa = 3.39 QPNTLLIYY515 pKa = 10.19 VYY517 pKa = 11.21 SDD519 pKa = 2.97 GTTEE523 pKa = 3.76 KK524 pKa = 10.55 VFRR527 pKa = 11.84 VEE529 pKa = 3.78
Molecular weight: 56.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.872
IPC_protein 3.923
Toseland 3.681
ProMoST 4.088
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.897
Rodwell 3.745
Grimsley 3.592
Solomon 3.935
Lehninger 3.884
Nozaki 4.037
DTASelect 4.355
Thurlkill 3.745
EMBOSS 3.91
Sillero 4.05
Patrickios 1.43
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.924
Protein with the highest isoelectric point:
>tr|A0A5C6S045|A0A5C6S045_9FLAO DUF368 domain-containing protein OS=Vicingus serpentipes OX=1926625 GN=FRY74_02015 PE=4 SV=1
MM1 pKa = 7.5 AKK3 pKa = 10.25 EE4 pKa = 3.94 SMKK7 pKa = 10.47 ARR9 pKa = 11.84 EE10 pKa = 4.29 RR11 pKa = 11.84 KK12 pKa = 9.4 RR13 pKa = 11.84 EE14 pKa = 4.0 AMVEE18 pKa = 3.95 KK19 pKa = 10.81 YY20 pKa = 10.44 ADD22 pKa = 3.02 KK23 pKa = 10.81 RR24 pKa = 11.84 AALKK28 pKa = 10.63 AAGDD32 pKa = 3.42 WDD34 pKa = 4.21 ALQKK38 pKa = 10.78 LPKK41 pKa = 9.8 NSSKK45 pKa = 10.38 VRR47 pKa = 11.84 LHH49 pKa = 5.96 NRR51 pKa = 11.84 CKK53 pKa = 9.95 LTGRR57 pKa = 11.84 PRR59 pKa = 11.84 GYY61 pKa = 9.01 MRR63 pKa = 11.84 QFGISRR69 pKa = 11.84 VTFRR73 pKa = 11.84 EE74 pKa = 3.92 MASAGLIPGIKK85 pKa = 8.99 KK86 pKa = 10.68 ASWW89 pKa = 2.94
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.297
IPC2_protein 9.853
IPC_protein 10.613
Toseland 11.111
ProMoST 10.818
Dawson 11.155
Bjellqvist 10.847
Wikipedia 11.359
Rodwell 11.462
Grimsley 11.169
Solomon 11.33
Lehninger 11.286
Nozaki 11.082
DTASelect 10.847
Thurlkill 11.082
EMBOSS 11.506
Sillero 11.082
Patrickios 11.199
IPC_peptide 11.33
IPC2_peptide 9.692
IPC2.peptide.svr19 8.617
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2518
0
2518
911875
38
3426
362.1
40.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.045 ± 0.055
0.91 ± 0.023
5.535 ± 0.04
6.593 ± 0.065
5.168 ± 0.045
6.5 ± 0.06
1.6 ± 0.022
8.521 ± 0.05
7.922 ± 0.086
9.039 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.046 ± 0.025
6.885 ± 0.047
3.277 ± 0.032
3.152 ± 0.026
2.815 ± 0.036
6.774 ± 0.046
5.844 ± 0.077
6.155 ± 0.038
0.987 ± 0.016
4.231 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here