Methanococcus voltae (strain ATCC BAA-1334 / A3)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanococcaceae; Methanococcus; Methanococcus voltae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7DR98|D7DR98_METV3 Flagellin OS=Methanococcus voltae (strain ATCC BAA-1334 / A3) OX=456320 GN=Mvol_1720 PE=4 SV=1
MM1 pKa = 7.62KK2 pKa = 10.41NNNILKK8 pKa = 9.99IFLCVMVSLVVMCSGCVSSSSSDD31 pKa = 3.41SADD34 pKa = 3.05DD35 pKa = 3.64VVVEE39 pKa = 4.45KK40 pKa = 10.42TSTNDD45 pKa = 2.94NTEE48 pKa = 3.81PKK50 pKa = 10.21EE51 pKa = 4.06NEE53 pKa = 3.79EE54 pKa = 4.35TKK56 pKa = 10.86DD57 pKa = 3.48DD58 pKa = 4.16EE59 pKa = 4.51KK60 pKa = 11.22PKK62 pKa = 10.07TEE64 pKa = 4.02EE65 pKa = 3.9PVEE68 pKa = 4.13EE69 pKa = 4.53GLPDD73 pKa = 3.32EE74 pKa = 4.55YY75 pKa = 11.56VAVFYY80 pKa = 10.15EE81 pKa = 4.59AYY83 pKa = 8.33STKK86 pKa = 10.64SFDD89 pKa = 3.16GSYY92 pKa = 10.82GKK94 pKa = 10.65EE95 pKa = 3.43EE96 pKa = 3.88AADD99 pKa = 3.68GKK101 pKa = 10.92KK102 pKa = 10.63YY103 pKa = 10.71LIVDD107 pKa = 3.56ITVYY111 pKa = 11.17NNGYY115 pKa = 10.52DD116 pKa = 3.9EE117 pKa = 5.33ISVNPLYY124 pKa = 10.75FDD126 pKa = 5.56AIVDD130 pKa = 4.01GVEE133 pKa = 3.57YY134 pKa = 10.2DD135 pKa = 3.8YY136 pKa = 11.6SIDD139 pKa = 4.09SYY141 pKa = 11.77SLEE144 pKa = 4.03NDD146 pKa = 3.16LDD148 pKa = 4.26SVNLRR153 pKa = 11.84NGGKK157 pKa = 8.49TSGSLAFEE165 pKa = 4.28IPEE168 pKa = 4.84DD169 pKa = 3.78SSSTYY174 pKa = 10.44SIEE177 pKa = 4.1YY178 pKa = 10.58NPITWDD184 pKa = 3.43KK185 pKa = 11.53LNIIYY190 pKa = 10.59NPEE193 pKa = 3.93DD194 pKa = 3.51VDD196 pKa = 4.04IEE198 pKa = 4.25EE199 pKa = 4.37AQKK202 pKa = 10.99AAASSSNTGAEE213 pKa = 3.93KK214 pKa = 9.82STPTTSNDD222 pKa = 3.34DD223 pKa = 3.62TDD225 pKa = 4.07SDD227 pKa = 4.3SVNIITAATVTEE239 pKa = 4.19SFNYY243 pKa = 10.53NIGSYY248 pKa = 9.91EE249 pKa = 3.96YY250 pKa = 10.27TRR252 pKa = 11.84EE253 pKa = 4.08APSGKK258 pKa = 8.63TYY260 pKa = 10.76LITYY264 pKa = 10.17ISIEE268 pKa = 3.94NNGYY272 pKa = 10.5DD273 pKa = 4.35EE274 pKa = 5.39ISTSPLYY281 pKa = 10.63FDD283 pKa = 6.1AIVDD287 pKa = 4.11GVGYY291 pKa = 10.33SYY293 pKa = 11.73ASDD296 pKa = 3.52TYY298 pKa = 11.27SLEE301 pKa = 3.93NHH303 pKa = 6.55LEE305 pKa = 4.15SVDD308 pKa = 3.6LRR310 pKa = 11.84NGGKK314 pKa = 8.47TSGLLIYY321 pKa = 7.7EE322 pKa = 4.23IPKK325 pKa = 10.81GSDD328 pKa = 3.18SYY330 pKa = 11.26EE331 pKa = 4.09LEE333 pKa = 4.15YY334 pKa = 11.3DD335 pKa = 4.17PISWDD340 pKa = 3.31KK341 pKa = 11.67LNIKK345 pKa = 9.98YY346 pKa = 10.27EE347 pKa = 4.17YY348 pKa = 10.2ISWDD352 pKa = 3.64EE353 pKa = 4.03IEE355 pKa = 4.14

Molecular weight: 39.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7DQW1|D7DQW1_METV3 Uncharacterized protein OS=Methanococcus voltae (strain ATCC BAA-1334 / A3) OX=456320 GN=Mvol_1583 PE=4 SV=1
MM1 pKa = 7.82ASNKK5 pKa = 9.66PLGKK9 pKa = 10.15KK10 pKa = 9.18IRR12 pKa = 11.84LAKK15 pKa = 10.44ALNQNRR21 pKa = 11.84RR22 pKa = 11.84VPLFAVAKK30 pKa = 8.49TKK32 pKa = 10.68GAVRR36 pKa = 11.84SHH38 pKa = 6.01PKK40 pKa = 7.76MRR42 pKa = 11.84NWRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 5.59TLKK50 pKa = 10.45KK51 pKa = 10.72

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1658

0

1658

496543

33

2506

299.5

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.34 ± 0.071

1.348 ± 0.035

5.755 ± 0.042

7.718 ± 0.07

3.714 ± 0.039

6.206 ± 0.058

1.443 ± 0.021

9.561 ± 0.056

9.278 ± 0.092

9.016 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.03

7.398 ± 0.106

3.141 ± 0.034

2.06 ± 0.024

2.922 ± 0.045

6.137 ± 0.057

5.159 ± 0.063

6.342 ± 0.055

0.588 ± 0.015

4.318 ± 0.054
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski