Amino acid dipepetide frequency for Methanococcus voltae (strain ATCC BAA-1334 / A3)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.397AlaAla: 2.397 ± 0.13
0.81AlaCys: 0.81 ± 0.059
2.834AlaAsp: 2.834 ± 0.099
3.988AlaGlu: 3.988 ± 0.126
2.076AlaPhe: 2.076 ± 0.069
3.909AlaGly: 3.909 ± 0.108
0.856AlaHis: 0.856 ± 0.043
5.391AlaIle: 5.391 ± 0.107
4.886AlaLys: 4.886 ± 0.103
5.232AlaLeu: 5.232 ± 0.122
1.502AlaMet: 1.502 ± 0.053
2.876AlaAsn: 2.876 ± 0.082
1.613AlaPro: 1.613 ± 0.067
1.162AlaGln: 1.162 ± 0.053
1.704AlaArg: 1.704 ± 0.064
3.154AlaSer: 3.154 ± 0.106
2.88AlaThr: 2.88 ± 0.093
3.698AlaVal: 3.698 ± 0.1
0.338AlaTrp: 0.338 ± 0.028
2.096AlaTyr: 2.096 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.826CysAla: 0.826 ± 0.06
0.159CysCys: 0.159 ± 0.02
0.836CysAsp: 0.836 ± 0.044
0.928CysGlu: 0.928 ± 0.044
0.344CysPhe: 0.344 ± 0.027
1.466CysGly: 1.466 ± 0.083
0.264CysHis: 0.264 ± 0.022
1.17CysIle: 1.17 ± 0.059
1.148CysLys: 1.148 ± 0.051
0.818CysLeu: 0.818 ± 0.045
0.31CysMet: 0.31 ± 0.025
0.868CysAsn: 0.868 ± 0.051
0.92CysPro: 0.92 ± 0.068
0.236CysGln: 0.236 ± 0.02
0.381CysArg: 0.381 ± 0.027
0.751CysSer: 0.751 ± 0.042
0.685CysThr: 0.685 ± 0.038
0.836CysVal: 0.836 ± 0.053
0.062CysTrp: 0.062 ± 0.012
0.471CysTyr: 0.471 ± 0.037
0.0CysXaa: 0.0 ± 0.0
Asp
3.067AspAla: 3.067 ± 0.089
0.757AspCys: 0.757 ± 0.043
3.11AspAsp: 3.11 ± 0.101
5.051AspGlu: 5.051 ± 0.102
2.445AspPhe: 2.445 ± 0.082
3.259AspGly: 3.259 ± 0.091
0.614AspHis: 0.614 ± 0.032
6.187AspIle: 6.187 ± 0.105
4.91AspLys: 4.91 ± 0.113
5.756AspLeu: 5.756 ± 0.12
1.498AspMet: 1.498 ± 0.052
3.969AspAsn: 3.969 ± 0.112
1.398AspPro: 1.398 ± 0.054
0.562AspGln: 0.562 ± 0.041
1.541AspArg: 1.541 ± 0.063
3.029AspSer: 3.029 ± 0.079
2.709AspThr: 2.709 ± 0.088
4.249AspVal: 4.249 ± 0.102
0.405AspTrp: 0.405 ± 0.031
3.033AspTyr: 3.033 ± 0.08
0.0AspXaa: 0.0 ± 0.0
Glu
3.569GluAla: 3.569 ± 0.104
0.854GluCys: 0.854 ± 0.043
4.39GluAsp: 4.39 ± 0.106
6.489GluGlu: 6.489 ± 0.154
3.361GluPhe: 3.361 ± 0.098
3.647GluGly: 3.647 ± 0.093
1.015GluHis: 1.015 ± 0.045
7.818GluIle: 7.818 ± 0.156
7.8GluLys: 7.8 ± 0.163
8.15GluLeu: 8.15 ± 0.16
2.076GluMet: 2.076 ± 0.077
6.553GluAsn: 6.553 ± 0.131
1.692GluPro: 1.692 ± 0.058
1.79GluGln: 1.79 ± 0.058
2.167GluArg: 2.167 ± 0.081
3.542GluSer: 3.542 ± 0.086
3.585GluThr: 3.585 ± 0.107
4.408GluVal: 4.408 ± 0.095
0.427GluTrp: 0.427 ± 0.03
3.849GluTyr: 3.849 ± 0.104
0.0GluXaa: 0.0 ± 0.0
Phe
1.996PheAla: 1.996 ± 0.091
0.51PheCys: 0.51 ± 0.034
2.54PheAsp: 2.54 ± 0.075
3.452PheGlu: 3.452 ± 0.085
1.202PhePhe: 1.202 ± 0.056
2.822PheGly: 2.822 ± 0.092
0.473PheHis: 0.473 ± 0.034
3.128PheIle: 3.128 ± 0.097
3.685PheLys: 3.685 ± 0.09
3.12PheLeu: 3.12 ± 0.088
1.081PheMet: 1.081 ± 0.053
2.463PheAsn: 2.463 ± 0.074
1.037PhePro: 1.037 ± 0.043
0.735PheGln: 0.735 ± 0.051
1.067PheArg: 1.067 ± 0.045
2.366PheSer: 2.366 ± 0.065
1.813PheThr: 1.813 ± 0.069
2.123PheVal: 2.123 ± 0.068
0.284PheTrp: 0.284 ± 0.026
1.247PheTyr: 1.247 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
4.044GlyAla: 4.044 ± 0.131
1.047GlyCys: 1.047 ± 0.048
3.222GlyAsp: 3.222 ± 0.092
3.681GlyGlu: 3.681 ± 0.098
2.564GlyPhe: 2.564 ± 0.068
3.99GlyGly: 3.99 ± 0.108
1.106GlyHis: 1.106 ± 0.056
6.577GlyIle: 6.577 ± 0.135
5.297GlyLys: 5.297 ± 0.123
5.476GlyLeu: 5.476 ± 0.128
1.591GlyMet: 1.591 ± 0.07
3.667GlyAsn: 3.667 ± 0.093
1.281GlyPro: 1.281 ± 0.054
1.323GlyGln: 1.323 ± 0.052
2.08GlyArg: 2.08 ± 0.067
3.557GlySer: 3.557 ± 0.091
3.833GlyThr: 3.833 ± 0.116
4.457GlyVal: 4.457 ± 0.107
0.413GlyTrp: 0.413 ± 0.03
2.856GlyTyr: 2.856 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
0.793HisAla: 0.793 ± 0.044
0.336HisCys: 0.336 ± 0.03
0.711HisAsp: 0.711 ± 0.044
0.838HisGlu: 0.838 ± 0.041
0.572HisPhe: 0.572 ± 0.035
0.955HisGly: 0.955 ± 0.049
0.316HisHis: 0.316 ± 0.026
1.388HisIle: 1.388 ± 0.05
1.188HisLys: 1.188 ± 0.05
1.136HisLeu: 1.136 ± 0.049
0.377HisMet: 0.377 ± 0.03
1.031HisAsn: 1.031 ± 0.05
0.729HisPro: 0.729 ± 0.042
0.312HisGln: 0.312 ± 0.024
0.489HisArg: 0.489 ± 0.031
0.898HisSer: 0.898 ± 0.051
0.802HisThr: 0.802 ± 0.044
0.836HisVal: 0.836 ± 0.039
0.081HisTrp: 0.081 ± 0.013
0.638HisTyr: 0.638 ± 0.034
0.0HisXaa: 0.0 ± 0.0
Ile
5.641IleAla: 5.641 ± 0.108
1.301IleCys: 1.301 ± 0.056
5.863IleAsp: 5.863 ± 0.113
6.787IleGlu: 6.787 ± 0.139
3.341IlePhe: 3.341 ± 0.095
5.601IleGly: 5.601 ± 0.12
1.418IleHis: 1.418 ± 0.054
8.678IleIle: 8.678 ± 0.154
7.913IleLys: 7.913 ± 0.122
8.577IleLeu: 8.577 ± 0.164
2.276IleMet: 2.276 ± 0.084
6.676IleAsn: 6.676 ± 0.163
3.641IlePro: 3.641 ± 0.096
2.096IleGln: 2.096 ± 0.073
2.461IleArg: 2.461 ± 0.079
6.789IleSer: 6.789 ± 0.14
6.169IleThr: 6.169 ± 0.133
6.024IleVal: 6.024 ± 0.1
0.514IleTrp: 0.514 ± 0.032
3.841IleTyr: 3.841 ± 0.093
0.0IleXaa: 0.0 ± 0.0
Lys
4.056LysAla: 4.056 ± 0.104
1.112LysCys: 1.112 ± 0.063
5.722LysAsp: 5.722 ± 0.113
8.124LysGlu: 8.124 ± 0.164
3.128LysPhe: 3.128 ± 0.095
4.551LysGly: 4.551 ± 0.111
1.196LysHis: 1.196 ± 0.058
9.359LysIle: 9.359 ± 0.164
8.767LysLys: 8.767 ± 0.181
8.368LysLeu: 8.368 ± 0.152
2.511LysMet: 2.511 ± 0.077
8.322LysAsn: 8.322 ± 0.163
2.606LysPro: 2.606 ± 0.074
1.76LysGln: 1.76 ± 0.057
2.777LysArg: 2.777 ± 0.076
5.327LysSer: 5.327 ± 0.119
4.719LysThr: 4.719 ± 0.102
5.244LysVal: 5.244 ± 0.094
0.497LysTrp: 0.497 ± 0.033
4.634LysTyr: 4.634 ± 0.121
0.0LysXaa: 0.0 ± 0.0
Leu
4.916LeuAla: 4.916 ± 0.12
1.148LeuCys: 1.148 ± 0.051
5.339LeuAsp: 5.339 ± 0.116
6.811LeuGlu: 6.811 ± 0.107
3.196LeuPhe: 3.196 ± 0.099
5.754LeuGly: 5.754 ± 0.13
1.245LeuHis: 1.245 ± 0.054
8.104LeuIle: 8.104 ± 0.152
10.448LeuLys: 10.448 ± 0.196
7.47LeuLeu: 7.47 ± 0.17
2.393LeuMet: 2.393 ± 0.078
7.103LeuAsn: 7.103 ± 0.136
2.781LeuPro: 2.781 ± 0.074
1.732LeuGln: 1.732 ± 0.057
2.729LeuArg: 2.729 ± 0.086
6.263LeuSer: 6.263 ± 0.134
4.485LeuThr: 4.485 ± 0.105
4.717LeuVal: 4.717 ± 0.126
0.483LeuTrp: 0.483 ± 0.036
3.043LeuTyr: 3.043 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.831MetAla: 1.831 ± 0.077
0.286MetCys: 0.286 ± 0.023
1.972MetAsp: 1.972 ± 0.065
1.945MetGlu: 1.945 ± 0.063
0.824MetPhe: 0.824 ± 0.044
2.31MetGly: 2.31 ± 0.073
0.409MetHis: 0.409 ± 0.03
2.131MetIle: 2.131 ± 0.074
1.996MetLys: 1.996 ± 0.061
1.889MetLeu: 1.889 ± 0.063
0.596MetMet: 0.596 ± 0.038
1.641MetAsn: 1.641 ± 0.058
0.926MetPro: 0.926 ± 0.043
0.56MetGln: 0.56 ± 0.037
0.717MetArg: 0.717 ± 0.039
1.378MetSer: 1.378 ± 0.053
1.289MetThr: 1.289 ± 0.053
1.847MetVal: 1.847 ± 0.064
0.115MetTrp: 0.115 ± 0.016
0.902MetTyr: 0.902 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
3.208AsnAla: 3.208 ± 0.113
0.971AsnCys: 0.971 ± 0.045
3.812AsnAsp: 3.812 ± 0.11
5.387AsnGlu: 5.387 ± 0.111
2.435AsnPhe: 2.435 ± 0.078
3.863AsnGly: 3.863 ± 0.095
0.858AsnHis: 0.858 ± 0.041
7.244AsnIle: 7.244 ± 0.178
6.696AsnLys: 6.696 ± 0.166
6.557AsnLeu: 6.557 ± 0.129
1.817AsnMet: 1.817 ± 0.056
7.613AsnAsn: 7.613 ± 0.274
2.743AsnPro: 2.743 ± 0.079
1.523AsnGln: 1.523 ± 0.06
1.851AsnArg: 1.851 ± 0.079
5.007AsnSer: 5.007 ± 0.132
4.074AsnThr: 4.074 ± 0.123
4.447AsnVal: 4.447 ± 0.118
0.419AsnTrp: 0.419 ± 0.034
3.452AsnTyr: 3.452 ± 0.096
0.0AsnXaa: 0.0 ± 0.0
Pro
1.617ProAla: 1.617 ± 0.062
0.479ProCys: 0.479 ± 0.041
1.462ProAsp: 1.462 ± 0.058
2.936ProGlu: 2.936 ± 0.08
1.335ProPhe: 1.335 ± 0.054
1.549ProGly: 1.549 ± 0.062
0.592ProHis: 0.592 ± 0.033
2.926ProIle: 2.926 ± 0.076
2.838ProLys: 2.838 ± 0.087
2.866ProLeu: 2.866 ± 0.072
0.814ProMet: 0.814 ± 0.041
1.875ProAsn: 1.875 ± 0.069
0.81ProPro: 0.81 ± 0.038
0.763ProGln: 0.763 ± 0.034
0.862ProArg: 0.862 ± 0.048
1.853ProSer: 1.853 ± 0.059
1.716ProThr: 1.716 ± 0.071
2.542ProVal: 2.542 ± 0.067
0.173ProTrp: 0.173 ± 0.019
1.398ProTyr: 1.398 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
0.965GlnAla: 0.965 ± 0.045
0.264GlnCys: 0.264 ± 0.024
1.051GlnAsp: 1.051 ± 0.043
1.498GlnGlu: 1.498 ± 0.065
0.777GlnPhe: 0.777 ± 0.045
1.15GlnGly: 1.15 ± 0.05
0.28GlnHis: 0.28 ± 0.025
2.036GlnIle: 2.036 ± 0.069
2.175GlnLys: 2.175 ± 0.069
1.802GlnLeu: 1.802 ± 0.065
0.669GlnMet: 0.669 ± 0.033
1.647GlnAsn: 1.647 ± 0.063
0.556GlnPro: 0.556 ± 0.039
0.542GlnGln: 0.542 ± 0.041
0.757GlnArg: 0.757 ± 0.042
1.104GlnSer: 1.104 ± 0.042
0.914GlnThr: 0.914 ± 0.045
1.237GlnVal: 1.237 ± 0.053
0.141GlnTrp: 0.141 ± 0.016
1.037GlnTyr: 1.037 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
1.76ArgAla: 1.76 ± 0.061
0.455ArgCys: 0.455 ± 0.035
1.674ArgAsp: 1.674 ± 0.063
2.078ArgGlu: 2.078 ± 0.066
1.237ArgPhe: 1.237 ± 0.05
1.825ArgGly: 1.825 ± 0.08
0.501ArgHis: 0.501 ± 0.032
2.652ArgIle: 2.652 ± 0.069
2.614ArgLys: 2.614 ± 0.072
2.562ArgLeu: 2.562 ± 0.066
0.84ArgMet: 0.84 ± 0.043
1.821ArgAsn: 1.821 ± 0.063
0.934ArgPro: 0.934 ± 0.053
0.642ArgGln: 0.642 ± 0.037
1.235ArgArg: 1.235 ± 0.057
1.373ArgSer: 1.373 ± 0.056
1.392ArgThr: 1.392 ± 0.062
2.006ArgVal: 2.006 ± 0.075
0.205ArgTrp: 0.205 ± 0.021
1.414ArgTyr: 1.414 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
3.156SerAla: 3.156 ± 0.088
0.693SerCys: 0.693 ± 0.04
3.176SerAsp: 3.176 ± 0.087
5.127SerGlu: 5.127 ± 0.105
2.304SerPhe: 2.304 ± 0.104
3.877SerGly: 3.877 ± 0.099
0.804SerHis: 0.804 ± 0.04
5.583SerIle: 5.583 ± 0.123
6.948SerLys: 6.948 ± 0.133
5.24SerLeu: 5.24 ± 0.1
1.382SerMet: 1.382 ± 0.055
4.501SerAsn: 4.501 ± 0.113
1.73SerPro: 1.73 ± 0.054
1.365SerGln: 1.365 ± 0.053
1.766SerArg: 1.766 ± 0.065
4.02SerSer: 4.02 ± 0.138
3.347SerThr: 3.347 ± 0.098
3.714SerVal: 3.714 ± 0.099
0.338SerTrp: 0.338 ± 0.029
2.304SerTyr: 2.304 ± 0.07
0.0SerXaa: 0.0 ± 0.0
Thr
2.985ThrAla: 2.985 ± 0.093
0.616ThrCys: 0.616 ± 0.035
2.58ThrAsp: 2.58 ± 0.081
3.448ThrGlu: 3.448 ± 0.083
1.863ThrPhe: 1.863 ± 0.069
3.688ThrGly: 3.688 ± 0.096
0.806ThrHis: 0.806 ± 0.046
4.924ThrIle: 4.924 ± 0.127
4.286ThrLys: 4.286 ± 0.103
4.839ThrLeu: 4.839 ± 0.128
1.204ThrMet: 1.204 ± 0.05
3.615ThrAsn: 3.615 ± 0.138
2.141ThrPro: 2.141 ± 0.068
1.122ThrGln: 1.122 ± 0.051
1.371ThrArg: 1.371 ± 0.055
3.085ThrSer: 3.085 ± 0.09
3.144ThrThr: 3.144 ± 0.107
3.48ThrVal: 3.48 ± 0.101
0.276ThrTrp: 0.276 ± 0.025
2.123ThrTyr: 2.123 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
3.901ValAla: 3.901 ± 0.106
0.957ValCys: 0.957 ± 0.052
3.826ValAsp: 3.826 ± 0.082
4.916ValGlu: 4.916 ± 0.106
2.344ValPhe: 2.344 ± 0.071
4.314ValGly: 4.314 ± 0.111
0.961ValHis: 0.961 ± 0.044
6.092ValIle: 6.092 ± 0.133
5.44ValLys: 5.44 ± 0.103
5.941ValLeu: 5.941 ± 0.11
1.424ValMet: 1.424 ± 0.059
4.092ValAsn: 4.092 ± 0.104
2.231ValPro: 2.231 ± 0.072
1.41ValGln: 1.41 ± 0.063
1.899ValArg: 1.899 ± 0.067
4.789ValSer: 4.789 ± 0.107
1.579ValThr: 1.579 ± 0.089
4.485ValVal: 4.485 ± 0.131
0.363ValTrp: 0.363 ± 0.029
2.455ValTyr: 2.455 ± 0.081
0.0ValXaa: 0.0 ± 0.0
Trp
0.375TrpAla: 0.375 ± 0.028
0.083TrpCys: 0.083 ± 0.015
0.451TrpAsp: 0.451 ± 0.036
0.463TrpGlu: 0.463 ± 0.032
0.175TrpPhe: 0.175 ± 0.019
0.427TrpGly: 0.427 ± 0.031
0.121TrpHis: 0.121 ± 0.018
0.503TrpIle: 0.503 ± 0.03
0.489TrpLys: 0.489 ± 0.037
0.427TrpLeu: 0.427 ± 0.032
0.183TrpMet: 0.183 ± 0.02
0.415TrpAsn: 0.415 ± 0.032
0.105TrpPro: 0.105 ± 0.015
0.141TrpGln: 0.141 ± 0.016
0.193TrpArg: 0.193 ± 0.021
0.288TrpSer: 0.288 ± 0.024
0.282TrpThr: 0.282 ± 0.024
0.453TrpVal: 0.453 ± 0.028
0.056TrpTrp: 0.056 ± 0.01
0.248TrpTyr: 0.248 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.3TyrAla: 2.3 ± 0.07
0.642TyrCys: 0.642 ± 0.039
2.979TyrAsp: 2.979 ± 0.098
3.234TyrGlu: 3.234 ± 0.098
1.619TyrPhe: 1.619 ± 0.053
3.107TyrGly: 3.107 ± 0.084
0.592TyrHis: 0.592 ± 0.034
3.714TyrIle: 3.714 ± 0.081
3.257TyrLys: 3.257 ± 0.097
3.933TyrLeu: 3.933 ± 0.101
1.017TyrMet: 1.017 ± 0.046
3.226TyrAsn: 3.226 ± 0.106
1.531TyrPro: 1.531 ± 0.064
0.828TyrGln: 0.828 ± 0.047
1.172TyrArg: 1.172 ± 0.053
2.801TyrSer: 2.801 ± 0.074
2.181TyrThr: 2.181 ± 0.072
2.618TyrVal: 2.618 ± 0.078
0.288TyrTrp: 0.288 ± 0.025
2.141TyrTyr: 2.141 ± 0.087
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1658 proteins (496543 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski