Sistotremastrum niveocremeum HHB9708
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13037 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A164RVU1|A0A164RVU1_9AGAM Uncharacterized protein OS=Sistotremastrum niveocremeum HHB9708 OX=1314777 GN=SISNIDRAFT_468170 PE=4 SV=1
VV1 pKa = 7.84 DD2 pKa = 4.52 DD3 pKa = 5.55 ASDD6 pKa = 3.54 VTYY9 pKa = 7.56 TTTMNIGGRR18 pKa = 11.84 DD19 pKa = 3.28 ISVLIDD25 pKa = 3.37 TGSSDD30 pKa = 3.7 LWATGQIPNSTPTNFTADD48 pKa = 3.16 VTYY51 pKa = 10.8 AQGDD55 pKa = 3.41 AGGYY59 pKa = 9.64 ISIAPVQFAGYY70 pKa = 7.27 TIPDD74 pKa = 3.23 QAYY77 pKa = 9.01 INVTSGDD84 pKa = 3.93 PPSVDD89 pKa = 3.15 GLIGLGPYY97 pKa = 8.83 MGSTIRR103 pKa = 11.84 TTVGSPSADD112 pKa = 2.91 PVLNRR117 pKa = 11.84 IFAQNTSTPNFLSFILGRR135 pKa = 11.84 SSGPSQPFSGEE146 pKa = 3.91 LTVGEE151 pKa = 4.46 VIQGFEE157 pKa = 4.35 KK158 pKa = 10.53 ISGEE162 pKa = 4.25 VKK164 pKa = 10.85 NEE166 pKa = 3.85 VQVLPTSDD174 pKa = 5.06 SGDD177 pKa = 3.55 QHH179 pKa = 6.37 WALSLDD185 pKa = 3.98 TFTGPDD191 pKa = 3.5 GKK193 pKa = 10.53 AIPYY197 pKa = 6.68 TTTVPNEE204 pKa = 4.24 SKK206 pKa = 10.47 PVAVLDD212 pKa = 4.11 SGFSLPQIPGNISDD226 pKa = 5.26 AIYY229 pKa = 10.78 GRR231 pKa = 11.84 VQGAQWLTTSPVGPVWQIPCEE252 pKa = 3.97 QEE254 pKa = 4.28 LNISVTIGGKK264 pKa = 9.69 VYY266 pKa = 9.92 PIHH269 pKa = 6.73 PLDD272 pKa = 3.58 VSLEE276 pKa = 3.87 ITINPDD282 pKa = 2.62 IPNICVGAFQPSTIGFSNAAQIFDD306 pKa = 4.09 MVLGMSFLRR315 pKa = 11.84 NTYY318 pKa = 7.84 TLINFGDD325 pKa = 4.0 FVDD328 pKa = 4.3 VNSSVTADD336 pKa = 4.01 PYY338 pKa = 10.89 IQLLSLTDD346 pKa = 3.89 PGQAHH351 pKa = 6.28 QDD353 pKa = 3.73 FVSTRR358 pKa = 11.84 LNNVDD363 pKa = 3.77 TTSDD367 pKa = 3.45 SQFALLPASSQQSSS381 pKa = 3.05
Molecular weight: 40.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.795
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.643
Grimsley 3.49
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.24
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.948
Patrickios 1.914
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|A0A165A9D2|A0A165A9D2_9AGAM Cytochrome P450 OS=Sistotremastrum niveocremeum HHB9708 OX=1314777 GN=SISNIDRAFT_481369 PE=3 SV=1
MM1 pKa = 7.39 PRR3 pKa = 11.84 IPFSLSRR10 pKa = 11.84 LLARR14 pKa = 11.84 PLPIVQRR21 pKa = 11.84 QPLVNRR27 pKa = 11.84 SVSLTTHH34 pKa = 7.08 LSSSIPRR41 pKa = 11.84 LSSSPILRR49 pKa = 11.84 AVSLLRR55 pKa = 11.84 SSISLLPTTPTASHH69 pKa = 7.22 LLQVRR74 pKa = 11.84 FNQRR78 pKa = 11.84 GVDD81 pKa = 3.75 YY82 pKa = 10.92 QPSQRR87 pKa = 11.84 KK88 pKa = 8.81 RR89 pKa = 11.84 KK90 pKa = 9.17 RR91 pKa = 11.84 KK92 pKa = 9.36 HH93 pKa = 5.58 GFLARR98 pKa = 11.84 LRR100 pKa = 11.84 SAGGRR105 pKa = 11.84 KK106 pKa = 8.73 ILARR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 AKK114 pKa = 9.28 GRR116 pKa = 11.84 KK117 pKa = 8.14 YY118 pKa = 10.54 LSHH121 pKa = 7.04
Molecular weight: 13.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.404
IPC2_protein 11.082
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.237
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.959
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13030
7
13037
5547635
50
4925
425.5
47.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.036 ± 0.018
1.249 ± 0.009
5.567 ± 0.012
5.937 ± 0.018
3.873 ± 0.014
6.161 ± 0.021
2.605 ± 0.009
5.257 ± 0.015
4.507 ± 0.017
9.582 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.971 ± 0.007
3.483 ± 0.011
6.556 ± 0.029
3.687 ± 0.013
6.157 ± 0.017
9.156 ± 0.03
6.009 ± 0.014
6.123 ± 0.014
1.477 ± 0.008
2.606 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here