Faecalibacterium prausnitzii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Faecalibacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A173XAR1|A0A173XAR1_9FIRM ATP phosphoribosyltransferase OS=Faecalibacterium prausnitzii OX=853 GN=hisG PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 10.0FWKK5 pKa = 8.97RR6 pKa = 11.84TALCLALCAALLAGCTPARR25 pKa = 11.84DD26 pKa = 3.78AAEE29 pKa = 4.43SDD31 pKa = 3.9TPADD35 pKa = 3.75PLTGQEE41 pKa = 4.65LLWPGQRR48 pKa = 11.84PVAVTIEE55 pKa = 4.27NDD57 pKa = 3.57PSSTTQWGLSTASVVLQALTEE78 pKa = 4.4TQTPTSLCLVYY89 pKa = 9.89PAVDD93 pKa = 3.48AVPQVGPVSAGQDD106 pKa = 3.71LYY108 pKa = 10.94WRR110 pKa = 11.84LLVGQQVLPVQRR122 pKa = 11.84GGGAFDD128 pKa = 3.77QNYY131 pKa = 10.12LDD133 pKa = 4.29YY134 pKa = 11.26YY135 pKa = 9.13SLRR138 pKa = 11.84VVDD141 pKa = 4.86ALEE144 pKa = 4.28TGTNAFSCDD153 pKa = 3.86GAWSNSPLWYY163 pKa = 9.58TSGSALAGVLGKK175 pKa = 10.84LNISSVLTEE184 pKa = 4.3SRR186 pKa = 11.84LTDD189 pKa = 3.44AAASGTSEE197 pKa = 4.55SEE199 pKa = 4.14TEE201 pKa = 4.18TTVLSVPALLPQQTKK216 pKa = 9.71GHH218 pKa = 6.69LPDD221 pKa = 4.61AAASDD226 pKa = 3.92AVSVRR231 pKa = 11.84VQFDD235 pKa = 3.08ASNATGFSYY244 pKa = 11.04DD245 pKa = 4.35ADD247 pKa = 3.59SSTYY251 pKa = 11.2KK252 pKa = 9.8MLHH255 pKa = 6.79ADD257 pKa = 3.94GTPQLDD263 pKa = 3.79ANNGQQAGFDD273 pKa = 3.54NLLVLFSASALRR285 pKa = 11.84DD286 pKa = 3.61DD287 pKa = 5.71GLTLDD292 pKa = 4.48YY293 pKa = 10.81DD294 pKa = 3.78LTMGGGVWLNGGHH307 pKa = 7.62LWPITWTQGSDD318 pKa = 3.17TTFFFYY324 pKa = 10.82DD325 pKa = 3.73ADD327 pKa = 4.13GQPLTLTAGSSYY339 pKa = 10.38IALVSSLTGQEE350 pKa = 4.15LMVLNSAGEE359 pKa = 4.33SLTPALATEE368 pKa = 4.69ADD370 pKa = 3.87SASINPP376 pKa = 3.84

Molecular weight: 39.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A173XXL3|A0A173XXL3_9FIRM Ribosomal large subunit pseudouridine synthase D OS=Faecalibacterium prausnitzii OX=853 GN=rluD_2 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.47KK9 pKa = 7.63RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.09EE14 pKa = 3.35VHH16 pKa = 5.99GFLTRR21 pKa = 11.84MSTKK25 pKa = 10.35NGRR28 pKa = 11.84KK29 pKa = 9.33VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.97SLTVV44 pKa = 3.12

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2653

0

2653

826170

29

3304

311.4

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.043 ± 0.07

1.7 ± 0.019

5.567 ± 0.039

6.77 ± 0.052

3.803 ± 0.035

7.445 ± 0.047

1.887 ± 0.023

5.426 ± 0.045

5.426 ± 0.046

9.862 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.025

3.66 ± 0.028

4.089 ± 0.03

3.584 ± 0.031

5.177 ± 0.047

5.453 ± 0.04

5.676 ± 0.037

7.034 ± 0.042

1.05 ± 0.018

3.58 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski