Streptomyces boncukensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7299 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G4WU91|A0A6G4WU91_9ACTN GNAT family N-acetyltransferase OS=Streptomyces boncukensis OX=2711219 GN=G5C65_10885 PE=4 SV=1
MM1 pKa = 7.6FPTLDD6 pKa = 4.11DD7 pKa = 4.25LPAIVASRR15 pKa = 11.84PSDD18 pKa = 3.42QQYY21 pKa = 11.38APLLVDD27 pKa = 3.68PANARR32 pKa = 11.84VVRR35 pKa = 11.84ADD37 pKa = 3.48EE38 pKa = 4.38VKK40 pKa = 11.01AGDD43 pKa = 3.75TVLAAVDD50 pKa = 3.72SRR52 pKa = 11.84EE53 pKa = 4.08GGFDD57 pKa = 3.42VDD59 pKa = 3.83WFEE62 pKa = 4.03EE63 pKa = 4.72AYY65 pKa = 10.46AADD68 pKa = 4.53PQPFDD73 pKa = 3.85PTCQCGACGLADD85 pKa = 3.79PAEE88 pKa = 4.58GEE90 pKa = 4.5TIVLCTDD97 pKa = 3.35SASYY101 pKa = 10.75GPSLTCDD108 pKa = 3.28PWPAARR114 pKa = 11.84LVLVVPAA121 pKa = 4.94

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G4X8A1|A0A6G4X8A1_9ACTN SDR family NAD(P)-dependent oxidoreductase (Fragment) OS=Streptomyces boncukensis OX=2711219 GN=G5C65_33005 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVANRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.06GRR40 pKa = 11.84TRR42 pKa = 11.84LSAA45 pKa = 3.71

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7299

0

7299

2369374

29

5476

324.6

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.137 ± 0.044

0.83 ± 0.008

5.932 ± 0.023

5.988 ± 0.028

2.604 ± 0.016

9.897 ± 0.028

2.333 ± 0.016

2.731 ± 0.02

1.88 ± 0.025

10.434 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.657 ± 0.011

1.55 ± 0.014

6.259 ± 0.023

2.712 ± 0.016

8.718 ± 0.031

4.926 ± 0.02

5.747 ± 0.022

8.155 ± 0.029

1.498 ± 0.013

2.012 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski