Serratia odorifera DSM 4582
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5121 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D4E3H3|D4E3H3_SEROD Fatty acid oxidation complex subunit alpha OS=Serratia odorifera DSM 4582 OX=667129 GN=fadJ PE=3 SV=1
MM1 pKa = 8.18 DD2 pKa = 4.43 NTDD5 pKa = 3.4 CTASYY10 pKa = 10.71 SQVFTDD16 pKa = 3.76 QQQAQQALAALTDD29 pKa = 3.43 KK30 pKa = 11.12 ARR32 pKa = 11.84 AVEE35 pKa = 4.99 SDD37 pKa = 3.27 PCDD40 pKa = 3.31 INSSINPVDD49 pKa = 3.88 GGFQLDD55 pKa = 3.21 VDD57 pKa = 6.34 FIFCCQAEE65 pKa = 4.29 TLIFQLGLRR74 pKa = 4.32
Molecular weight: 8.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.617
IPC_protein 3.554
Toseland 3.338
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.605
Rodwell 3.401
Grimsley 3.261
Solomon 3.541
Lehninger 3.503
Nozaki 3.732
DTASelect 3.999
Thurlkill 3.439
EMBOSS 3.605
Sillero 3.694
Patrickios 0.604
IPC_peptide 3.541
IPC2_peptide 3.656
IPC2.peptide.svr19 3.676
Protein with the highest isoelectric point:
>tr|D4E049|D4E049_SEROD Homoserine/Threonine efflux protein OS=Serratia odorifera DSM 4582 OX=667129 GN=rhtB2 PE=4 SV=1
MM1 pKa = 7.31 KK2 pKa = 10.17 RR3 pKa = 11.84 NRR5 pKa = 11.84 SHH7 pKa = 7.03 GFRR10 pKa = 11.84 ARR12 pKa = 11.84 MATKK16 pKa = 10.4 NGRR19 pKa = 11.84 QVLARR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 AKK28 pKa = 10.41 GRR30 pKa = 11.84 TRR32 pKa = 11.84 LSVSKK37 pKa = 10.96
Molecular weight: 4.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.618
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.34
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.165
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5121
0
5121
1498009
31
3986
292.5
32.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.44 ± 0.042
1.074 ± 0.013
5.16 ± 0.027
5.124 ± 0.034
3.757 ± 0.028
7.64 ± 0.035
2.26 ± 0.016
5.52 ± 0.034
3.805 ± 0.034
11.222 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.705 ± 0.016
3.543 ± 0.026
4.581 ± 0.024
5.091 ± 0.031
5.93 ± 0.032
5.821 ± 0.027
5.073 ± 0.025
6.955 ± 0.027
1.46 ± 0.017
2.837 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here