Corynebacterium halotolerans YIM 70093 = DSM 44683
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2853 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1MYC0|M1MYC0_9CORY Membrane protein OS=Corynebacterium halotolerans YIM 70093 = DSM 44683 OX=1121362 GN=A605_08660 PE=4 SV=1
MM1 pKa = 7.8 SGLTLAATGVLAPTVTAQEE20 pKa = 4.32 DD21 pKa = 4.24 TATTAWEE28 pKa = 3.88 EE29 pKa = 3.64 TAYY32 pKa = 10.37 RR33 pKa = 11.84 GSVEE37 pKa = 4.68 VVDD40 pKa = 4.52 EE41 pKa = 4.16 QDD43 pKa = 5.11 DD44 pKa = 4.26 EE45 pKa = 4.49 NTLEE49 pKa = 4.13 GVVFEE54 pKa = 4.84 DD55 pKa = 3.94 TNKK58 pKa = 10.3 NSQRR62 pKa = 11.84 DD63 pKa = 3.62 EE64 pKa = 4.42 GEE66 pKa = 3.69 PGIPGVSVSNGRR78 pKa = 11.84 TIVTTDD84 pKa = 2.67 AEE86 pKa = 4.49 GRR88 pKa = 11.84 YY89 pKa = 8.76 EE90 pKa = 4.62 LQVDD94 pKa = 3.88 DD95 pKa = 4.28 NTTVFITQPAGYY107 pKa = 9.0 QVPVDD112 pKa = 4.28 EE113 pKa = 5.13 NNVAQFFYY121 pKa = 10.87 NHH123 pKa = 5.73 VPEE126 pKa = 5.05 GSPDD130 pKa = 3.27 LRR132 pKa = 11.84 YY133 pKa = 10.55 SGIEE137 pKa = 3.79 PTGPLPDD144 pKa = 3.76 AVNFPLSPSEE154 pKa = 4.02 GTASPEE160 pKa = 3.94 QNCVIGGDD168 pKa = 3.41 IQTYY172 pKa = 5.57 TAEE175 pKa = 4.11 EE176 pKa = 3.92 VEE178 pKa = 4.48 YY179 pKa = 11.02 ARR181 pKa = 11.84 NGAFADD187 pKa = 4.12 LAARR191 pKa = 11.84 DD192 pKa = 4.47 DD193 pKa = 4.05 YY194 pKa = 11.8 ANCGALFIGDD204 pKa = 3.83 VVGDD208 pKa = 4.43 DD209 pKa = 3.6 LSLYY213 pKa = 10.08 PDD215 pKa = 3.53 VKK217 pKa = 10.75 EE218 pKa = 3.91 LTGMLNGPARR228 pKa = 11.84 FLPGNHH234 pKa = 7.08 DD235 pKa = 4.3 LDD237 pKa = 4.62 FDD239 pKa = 5.63 ASDD242 pKa = 4.07 EE243 pKa = 4.03 DD244 pKa = 4.42 HH245 pKa = 7.2 KK246 pKa = 11.4 FDD248 pKa = 3.75 TYY250 pKa = 10.89 RR251 pKa = 11.84 SHH253 pKa = 7.73 FGPEE257 pKa = 4.15 YY258 pKa = 10.46 YY259 pKa = 10.81 SFDD262 pKa = 3.26 VGNAHH267 pKa = 6.16 VVALEE272 pKa = 4.03 SVQYY276 pKa = 10.18 PLEE279 pKa = 5.54 DD280 pKa = 3.88 GDD282 pKa = 4.76 GYY284 pKa = 11.56 NGAIDD289 pKa = 5.05 DD290 pKa = 4.69 EE291 pKa = 4.28 QLEE294 pKa = 4.4 WLRR297 pKa = 11.84 QDD299 pKa = 3.23 IAHH302 pKa = 6.12 TPEE305 pKa = 4.88 DD306 pKa = 4.05 KK307 pKa = 11.18 LIVLATHH314 pKa = 6.08 VPLMSFADD322 pKa = 3.61 QGSDD326 pKa = 2.99 VHH328 pKa = 8.11 QIDD331 pKa = 3.85 QVQEE335 pKa = 3.61 IYY337 pKa = 10.72 EE338 pKa = 4.31 IIGDD342 pKa = 3.93 RR343 pKa = 11.84 EE344 pKa = 4.21 AIAVSGHH351 pKa = 3.93 THH353 pKa = 6.69 ALANMRR359 pKa = 11.84 EE360 pKa = 4.07 GDD362 pKa = 4.2 SLEE365 pKa = 4.14 GWNEE369 pKa = 3.84 LYY371 pKa = 10.66 GVEE374 pKa = 4.51 EE375 pKa = 4.65 LPFTHH380 pKa = 6.63 IVAGAISGDD389 pKa = 3.6 WYY391 pKa = 11.09 SGEE394 pKa = 4.2 TTEE397 pKa = 5.2 NGYY400 pKa = 8.81 PQAVGRR406 pKa = 11.84 DD407 pKa = 3.51 GSLPGVLTLDD417 pKa = 3.19 IDD419 pKa = 3.93 GSEE422 pKa = 3.87 VSEE425 pKa = 4.96 FYY427 pKa = 9.99 TVRR430 pKa = 11.84 GEE432 pKa = 4.19 DD433 pKa = 3.37 QSTQMALSLNTPAYY447 pKa = 9.91 RR448 pKa = 11.84 DD449 pKa = 3.47 WYY451 pKa = 10.08 EE452 pKa = 4.67 DD453 pKa = 3.69 NIDD456 pKa = 3.69 NAGEE460 pKa = 4.07 AEE462 pKa = 4.37 EE463 pKa = 4.53 FADD466 pKa = 3.67 PLVVPEE472 pKa = 5.06 DD473 pKa = 3.84 EE474 pKa = 5.1 VAEE477 pKa = 4.33 TWLTTNFWMGSTGSTVEE494 pKa = 3.71 VSIDD498 pKa = 3.18 GGEE501 pKa = 3.8 AVAAEE506 pKa = 4.67 RR507 pKa = 11.84 TQQMDD512 pKa = 3.76 GEE514 pKa = 4.64 VPNVGAEE521 pKa = 3.94 WSDD524 pKa = 3.44 PAAVQQQLVHH534 pKa = 6.47 GGSVADD540 pKa = 4.32 RR541 pKa = 11.84 AAHH544 pKa = 6.53 IWRR547 pKa = 11.84 LPLPADD553 pKa = 4.37 LGAGEE558 pKa = 4.37 HH559 pKa = 5.13 TAEE562 pKa = 4.07 VTATDD567 pKa = 3.71 VHH569 pKa = 6.12 GHH571 pKa = 6.59 EE572 pKa = 4.46 YY573 pKa = 10.7 TEE575 pKa = 4.27 TLTFTVSPVEE585 pKa = 4.38 GGDD588 pKa = 4.58 DD589 pKa = 3.88 NGDD592 pKa = 4.14 DD593 pKa = 3.9 DD594 pKa = 4.95 TEE596 pKa = 4.91 APSSGLSSLSSNLSSAGSLFF616 pKa = 3.54
Molecular weight: 66.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.579
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.897
Patrickios 1.494
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|M1N1M5|M1N1M5_9CORY Uncharacterized protein OS=Corynebacterium halotolerans YIM 70093 = DSM 44683 OX=1121362 GN=A605_14212 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.73 GRR42 pKa = 11.84 AKK44 pKa = 9.63 LTAA47 pKa = 4.21
Molecular weight: 5.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2853
0
2853
924155
33
3023
323.9
34.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.941 ± 0.06
0.601 ± 0.011
6.302 ± 0.042
6.449 ± 0.048
3.137 ± 0.026
9.066 ± 0.044
2.259 ± 0.024
4.513 ± 0.034
2.235 ± 0.036
10.062 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.098 ± 0.02
2.371 ± 0.024
5.402 ± 0.036
2.901 ± 0.024
7.035 ± 0.043
5.242 ± 0.03
6.331 ± 0.032
8.576 ± 0.047
1.469 ± 0.021
2.01 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here