Chryseobacterium sp. (strain P1-3)
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A086F321|A0A086F321_CHRP1 LysM domain-containing protein OS=Chryseobacterium sp. (strain P1-3) OX=1517683 GN=HX13_21015 PE=4 SV=1
MM1 pKa = 7.9 DD2 pKa = 4.96 KK3 pKa = 10.41 EE4 pKa = 4.58 ISHH7 pKa = 6.95 FWLGYY12 pKa = 9.24 FKK14 pKa = 11.19 NEE16 pKa = 3.26 EE17 pKa = 3.74 DD18 pKa = 4.04 FYY20 pKa = 11.82 AFVEE24 pKa = 4.06 EE25 pKa = 4.47 DD26 pKa = 3.04 EE27 pKa = 4.68 NYY29 pKa = 10.32 YY30 pKa = 10.82 LEE32 pKa = 5.44 EE33 pKa = 4.13 EE34 pKa = 4.62 TEE36 pKa = 4.0 DD37 pKa = 3.6 QYY39 pKa = 12.09 VSKK42 pKa = 10.26 FAEE45 pKa = 4.11 SQNIKK50 pKa = 9.97 WFDD53 pKa = 3.73 DD54 pKa = 3.71 DD55 pKa = 4.26 FIEE58 pKa = 5.38 YY59 pKa = 10.48 GFEE62 pKa = 3.89 DD63 pKa = 4.3 EE64 pKa = 5.66 RR65 pKa = 11.84 LGIYY69 pKa = 10.1 EE70 pKa = 4.24 KK71 pKa = 10.72 FSEE74 pKa = 4.66 YY75 pKa = 10.74 SYY77 pKa = 11.56 ADD79 pKa = 2.83 QWLPLLEE86 pKa = 4.19 QKK88 pKa = 10.9 LNEE91 pKa = 4.84 LGLDD95 pKa = 3.62 TPVNAIIFASRR106 pKa = 11.84 FVIPNPVSVDD116 pKa = 3.23 GEE118 pKa = 4.43 EE119 pKa = 3.83 NKK121 pKa = 10.47 LYY123 pKa = 10.73 YY124 pKa = 9.86 IGEE127 pKa = 4.13 IEE129 pKa = 4.58 FEE131 pKa = 4.39 VV132 pKa = 3.47
Molecular weight: 15.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.656
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.024
Thurlkill 3.681
EMBOSS 3.681
Sillero 3.948
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.842
Protein with the highest isoelectric point:
>tr|A0A086F6F9|A0A086F6F9_CHRP1 TonB-dependent receptor OS=Chryseobacterium sp. (strain P1-3) OX=1517683 GN=HX13_10355 PE=3 SV=1
MM1 pKa = 7.75 IYY3 pKa = 10.33 FATKK7 pKa = 9.81 FEE9 pKa = 4.39 KK10 pKa = 10.43 VKK12 pKa = 10.52 IMKK15 pKa = 10.57 SMFKK19 pKa = 10.23 IIGAGVVMLMLSSCIVHH36 pKa = 6.29 EE37 pKa = 3.97 PHH39 pKa = 6.79 GRR41 pKa = 11.84 RR42 pKa = 11.84 MPPGHH47 pKa = 7.29 AKK49 pKa = 10.14 RR50 pKa = 11.84 FYY52 pKa = 10.33 GGSARR57 pKa = 11.84 YY58 pKa = 7.82 YY59 pKa = 11.11 APGHH63 pKa = 4.87 VKK65 pKa = 9.95 KK66 pKa = 11.03 VYY68 pKa = 8.86 IYY70 pKa = 9.92 EE71 pKa = 4.41 DD72 pKa = 3.39 RR73 pKa = 11.84 GHH75 pKa = 5.98 HH76 pKa = 5.43 HH77 pKa = 7.26 RR78 pKa = 11.84 GRR80 pKa = 11.84 GRR82 pKa = 11.84 GHH84 pKa = 6.32 HH85 pKa = 6.07 RR86 pKa = 3.44
Molecular weight: 9.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 10.087
IPC_protein 10.716
Toseland 10.687
ProMoST 10.555
Dawson 10.818
Bjellqvist 10.511
Wikipedia 11.023
Rodwell 11.169
Grimsley 10.877
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.511
Thurlkill 10.701
EMBOSS 11.082
Sillero 10.745
Patrickios 10.906
IPC_peptide 10.891
IPC2_peptide 9.428
IPC2.peptide.svr19 8.254
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3118
0
3118
889964
42
2742
285.4
32.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.358 ± 0.041
0.759 ± 0.013
5.302 ± 0.027
6.55 ± 0.054
5.232 ± 0.038
6.568 ± 0.043
1.803 ± 0.02
7.876 ± 0.045
8.002 ± 0.044
9.019 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.399 ± 0.024
6.108 ± 0.047
3.552 ± 0.028
3.594 ± 0.026
3.464 ± 0.027
6.478 ± 0.04
5.615 ± 0.042
5.982 ± 0.031
1.065 ± 0.016
4.278 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here