Mycobacterium phage Sheen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5A603|A0A0B5A603_9CAUD Uncharacterized protein OS=Mycobacterium phage Sheen OX=1589274 GN=83 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 5.41GLVATLLVALGIVLGAFTAAANCDD26 pKa = 3.83PFDD29 pKa = 3.7QASFPCQEE37 pKa = 4.6DD38 pKa = 3.84EE39 pKa = 4.52VLGYY43 pKa = 10.81APEE46 pKa = 4.51FGPDD50 pKa = 3.01KK51 pKa = 10.69VGCIHH56 pKa = 7.42IDD58 pKa = 3.34NLRR61 pKa = 3.87

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5A603|A0A0B5A603_9CAUD Uncharacterized protein OS=Mycobacterium phage Sheen OX=1589274 GN=83 PE=4 SV=1
MM1 pKa = 5.97TTAVVEE7 pKa = 5.16TPAKK11 pKa = 6.72MTPARR16 pKa = 11.84ALGITQGLLRR26 pKa = 11.84DD27 pKa = 4.05HH28 pKa = 6.81GLKK31 pKa = 9.95GWRR34 pKa = 11.84VQFDD38 pKa = 3.38NAKK41 pKa = 10.05RR42 pKa = 11.84RR43 pKa = 11.84AGQCNYY49 pKa = 9.29RR50 pKa = 11.84LSVISLSRR58 pKa = 11.84PLMARR63 pKa = 11.84RR64 pKa = 11.84SYY66 pKa = 10.87QDD68 pKa = 2.96TMNTITHH75 pKa = 7.19EE76 pKa = 4.04IAHH79 pKa = 6.49ALVGPNHH86 pKa = 6.19GHH88 pKa = 6.29DD89 pKa = 3.91AVWARR94 pKa = 11.84QHH96 pKa = 6.36KK97 pKa = 10.52ALGGDD102 pKa = 3.91GKK104 pKa = 11.04RR105 pKa = 11.84CFEE108 pKa = 4.33TDD110 pKa = 4.35DD111 pKa = 3.96VDD113 pKa = 5.56LTAPWIGTCEE123 pKa = 3.88HH124 pKa = 6.86GKK126 pKa = 9.69QFARR130 pKa = 11.84YY131 pKa = 8.13RR132 pKa = 11.84RR133 pKa = 11.84PKK135 pKa = 10.14RR136 pKa = 11.84LEE138 pKa = 3.58GWRR141 pKa = 11.84CRR143 pKa = 11.84CPQGSSSVVWSHH155 pKa = 6.39RR156 pKa = 11.84RR157 pKa = 3.24

Molecular weight: 17.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

16677

25

1126

198.5

21.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.54 ± 0.358

0.881 ± 0.124

6.59 ± 0.237

6.584 ± 0.274

3.184 ± 0.184

9.312 ± 0.715

2.075 ± 0.186

4.851 ± 0.158

4.197 ± 0.205

8.233 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.261 ± 0.122

3.358 ± 0.152

5.439 ± 0.305

3.712 ± 0.29

6.614 ± 0.423

4.965 ± 0.206

6.062 ± 0.224

7.232 ± 0.204

2.015 ± 0.162

2.896 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski