Amino acid dipepetide frequency for Mycobacterium phage Sheen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.974AlaAla: 10.974 ± 1.046
0.84AlaCys: 0.84 ± 0.185
6.117AlaAsp: 6.117 ± 0.609
5.577AlaGlu: 5.577 ± 0.761
3.178AlaPhe: 3.178 ± 0.532
8.875AlaGly: 8.875 ± 1.051
2.099AlaHis: 2.099 ± 0.328
4.078AlaIle: 4.078 ± 0.413
3.958AlaLys: 3.958 ± 0.504
8.455AlaLeu: 8.455 ± 0.792
2.339AlaMet: 2.339 ± 0.399
2.639AlaAsn: 2.639 ± 0.372
4.138AlaPro: 4.138 ± 0.44
3.718AlaGln: 3.718 ± 0.487
5.397AlaArg: 5.397 ± 0.729
4.557AlaSer: 4.557 ± 0.559
5.937AlaThr: 5.937 ± 0.463
7.856AlaVal: 7.856 ± 0.73
2.459AlaTrp: 2.459 ± 0.393
2.219AlaTyr: 2.219 ± 0.463
0.0AlaXaa: 0.0 ± 0.0
Cys
0.78CysAla: 0.78 ± 0.222
0.24CysCys: 0.24 ± 0.127
0.66CysAsp: 0.66 ± 0.172
0.72CysGlu: 0.72 ± 0.214
0.24CysPhe: 0.24 ± 0.12
0.899CysGly: 0.899 ± 0.25
0.36CysHis: 0.36 ± 0.165
0.12CysIle: 0.12 ± 0.075
0.42CysLys: 0.42 ± 0.157
0.66CysLeu: 0.66 ± 0.193
0.3CysMet: 0.3 ± 0.124
0.36CysAsn: 0.36 ± 0.118
0.42CysPro: 0.42 ± 0.163
0.42CysGln: 0.42 ± 0.165
0.66CysArg: 0.66 ± 0.229
0.18CysSer: 0.18 ± 0.11
0.36CysThr: 0.36 ± 0.18
0.54CysVal: 0.54 ± 0.188
0.3CysTrp: 0.3 ± 0.127
0.18CysTyr: 0.18 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
6.476AspAla: 6.476 ± 0.61
0.54AspCys: 0.54 ± 0.187
5.397AspAsp: 5.397 ± 0.738
4.438AspGlu: 4.438 ± 0.501
1.979AspPhe: 1.979 ± 0.41
5.997AspGly: 5.997 ± 0.848
1.559AspHis: 1.559 ± 0.343
2.219AspIle: 2.219 ± 0.457
2.758AspLys: 2.758 ± 0.455
5.877AspLeu: 5.877 ± 0.561
0.84AspMet: 0.84 ± 0.225
2.219AspAsn: 2.219 ± 0.359
4.438AspPro: 4.438 ± 0.563
1.919AspGln: 1.919 ± 0.311
3.478AspArg: 3.478 ± 0.443
3.358AspSer: 3.358 ± 0.392
4.198AspThr: 4.198 ± 0.451
4.917AspVal: 4.917 ± 0.472
1.439AspTrp: 1.439 ± 0.248
1.859AspTyr: 1.859 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
6.296GluAla: 6.296 ± 0.576
0.36GluCys: 0.36 ± 0.134
4.557GluAsp: 4.557 ± 0.516
4.258GluGlu: 4.258 ± 0.535
2.698GluPhe: 2.698 ± 0.424
5.757GluGly: 5.757 ± 0.797
1.079GluHis: 1.079 ± 0.241
3.058GluIle: 3.058 ± 0.419
2.519GluLys: 2.519 ± 0.396
6.296GluLeu: 6.296 ± 0.693
1.319GluMet: 1.319 ± 0.267
1.979GluAsn: 1.979 ± 0.289
2.579GluPro: 2.579 ± 0.438
3.178GluGln: 3.178 ± 0.537
4.378GluArg: 4.378 ± 0.675
2.758GluSer: 2.758 ± 0.417
3.778GluThr: 3.778 ± 0.564
5.337GluVal: 5.337 ± 0.466
1.559GluTrp: 1.559 ± 0.308
2.099GluTyr: 2.099 ± 0.356
0.0GluXaa: 0.0 ± 0.0
Phe
2.998PheAla: 2.998 ± 0.472
0.12PheCys: 0.12 ± 0.093
2.878PheAsp: 2.878 ± 0.41
1.859PheGlu: 1.859 ± 0.327
0.48PhePhe: 0.48 ± 0.152
3.538PheGly: 3.538 ± 0.422
0.54PheHis: 0.54 ± 0.145
1.319PheIle: 1.319 ± 0.389
1.259PheLys: 1.259 ± 0.271
2.399PheLeu: 2.399 ± 0.337
0.66PheMet: 0.66 ± 0.203
1.139PheAsn: 1.139 ± 0.244
1.679PhePro: 1.679 ± 0.351
1.199PheGln: 1.199 ± 0.252
1.859PheArg: 1.859 ± 0.318
1.979PheSer: 1.979 ± 0.353
2.279PheThr: 2.279 ± 0.331
2.279PheVal: 2.279 ± 0.403
0.72PheTrp: 0.72 ± 0.192
0.66PheTyr: 0.66 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
7.616GlyAla: 7.616 ± 1.127
0.959GlyCys: 0.959 ± 0.244
6.596GlyAsp: 6.596 ± 0.911
4.977GlyGlu: 4.977 ± 0.52
3.238GlyPhe: 3.238 ± 0.485
11.993GlyGly: 11.993 ± 3.7
2.159GlyHis: 2.159 ± 0.382
5.097GlyIle: 5.097 ± 0.532
4.378GlyLys: 4.378 ± 0.519
7.256GlyLeu: 7.256 ± 0.809
1.379GlyMet: 1.379 ± 0.306
3.358GlyAsn: 3.358 ± 0.587
5.697GlyPro: 5.697 ± 1.792
2.818GlyGln: 2.818 ± 0.461
4.557GlyArg: 4.557 ± 0.694
4.857GlySer: 4.857 ± 0.586
5.277GlyThr: 5.277 ± 0.732
6.057GlyVal: 6.057 ± 0.627
1.739GlyTrp: 1.739 ± 0.339
3.118GlyTyr: 3.118 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
2.039HisAla: 2.039 ± 0.361
0.12HisCys: 0.12 ± 0.09
1.439HisAsp: 1.439 ± 0.301
1.439HisGlu: 1.439 ± 0.303
0.84HisPhe: 0.84 ± 0.22
1.979HisGly: 1.979 ± 0.379
0.6HisHis: 0.6 ± 0.193
1.079HisIle: 1.079 ± 0.259
0.72HisLys: 0.72 ± 0.219
1.739HisLeu: 1.739 ± 0.41
0.3HisMet: 0.3 ± 0.115
0.42HisAsn: 0.42 ± 0.164
1.019HisPro: 1.019 ± 0.229
0.48HisGln: 0.48 ± 0.167
2.039HisArg: 2.039 ± 0.359
0.84HisSer: 0.84 ± 0.23
1.259HisThr: 1.259 ± 0.244
1.319HisVal: 1.319 ± 0.312
0.48HisTrp: 0.48 ± 0.17
0.6HisTyr: 0.6 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
5.097IleAla: 5.097 ± 0.508
0.36IleCys: 0.36 ± 0.143
3.898IleAsp: 3.898 ± 0.412
3.838IleGlu: 3.838 ± 0.355
1.019IlePhe: 1.019 ± 0.235
3.898IleGly: 3.898 ± 0.446
1.259IleHis: 1.259 ± 0.252
1.859IleIle: 1.859 ± 0.319
1.679IleLys: 1.679 ± 0.273
3.598IleLeu: 3.598 ± 0.427
0.66IleMet: 0.66 ± 0.2
1.919IleAsn: 1.919 ± 0.289
4.198IlePro: 4.198 ± 0.521
1.919IleGln: 1.919 ± 0.36
4.198IleArg: 4.198 ± 0.408
2.399IleSer: 2.399 ± 0.368
3.178IleThr: 3.178 ± 0.421
2.219IleVal: 2.219 ± 0.418
0.66IleTrp: 0.66 ± 0.192
0.66IleTyr: 0.66 ± 0.193
0.0IleXaa: 0.0 ± 0.0
Lys
4.677LysAla: 4.677 ± 0.587
0.18LysCys: 0.18 ± 0.095
2.339LysAsp: 2.339 ± 0.387
2.758LysGlu: 2.758 ± 0.404
1.139LysPhe: 1.139 ± 0.211
4.078LysGly: 4.078 ± 0.701
0.84LysHis: 0.84 ± 0.192
2.339LysIle: 2.339 ± 0.394
2.219LysLys: 2.219 ± 0.443
3.238LysLeu: 3.238 ± 0.372
1.319LysMet: 1.319 ± 0.317
1.259LysAsn: 1.259 ± 0.283
2.818LysPro: 2.818 ± 0.399
1.199LysGln: 1.199 ± 0.29
2.639LysArg: 2.639 ± 0.485
1.979LysSer: 1.979 ± 0.317
1.979LysThr: 1.979 ± 0.342
3.118LysVal: 3.118 ± 0.489
0.48LysTrp: 0.48 ± 0.154
1.379LysTyr: 1.379 ± 0.256
0.0LysXaa: 0.0 ± 0.0
Leu
8.695LeuAla: 8.695 ± 1.082
0.6LeuCys: 0.6 ± 0.241
5.337LeuAsp: 5.337 ± 0.533
4.797LeuGlu: 4.797 ± 0.52
2.099LeuPhe: 2.099 ± 0.326
6.836LeuGly: 6.836 ± 0.672
1.679LeuHis: 1.679 ± 0.322
4.198LeuIle: 4.198 ± 0.404
3.418LeuLys: 3.418 ± 0.479
5.817LeuLeu: 5.817 ± 0.583
2.579LeuMet: 2.579 ± 0.34
2.938LeuAsn: 2.938 ± 0.659
4.258LeuPro: 4.258 ± 0.546
2.758LeuGln: 2.758 ± 0.541
6.117LeuArg: 6.117 ± 0.629
4.677LeuSer: 4.677 ± 0.488
5.637LeuThr: 5.637 ± 0.742
5.997LeuVal: 5.997 ± 0.589
1.199LeuTrp: 1.199 ± 0.276
2.698LeuTyr: 2.698 ± 0.492
0.0LeuXaa: 0.0 ± 0.0
Met
2.219MetAla: 2.219 ± 0.408
0.12MetCys: 0.12 ± 0.072
1.199MetAsp: 1.199 ± 0.32
1.199MetGlu: 1.199 ± 0.298
0.54MetPhe: 0.54 ± 0.266
1.979MetGly: 1.979 ± 0.422
0.18MetHis: 0.18 ± 0.09
1.199MetIle: 1.199 ± 0.288
1.199MetLys: 1.199 ± 0.217
1.559MetLeu: 1.559 ± 0.368
0.24MetMet: 0.24 ± 0.094
0.959MetAsn: 0.959 ± 0.2
0.959MetPro: 0.959 ± 0.196
1.079MetGln: 1.079 ± 0.269
1.379MetArg: 1.379 ± 0.271
1.919MetSer: 1.919 ± 0.318
2.639MetThr: 2.639 ± 0.367
1.319MetVal: 1.319 ± 0.274
0.12MetTrp: 0.12 ± 0.086
0.6MetTyr: 0.6 ± 0.2
0.0MetXaa: 0.0 ± 0.0
Asn
3.238AsnAla: 3.238 ± 0.475
0.3AsnCys: 0.3 ± 0.148
1.439AsnAsp: 1.439 ± 0.311
2.039AsnGlu: 2.039 ± 0.306
1.079AsnPhe: 1.079 ± 0.231
3.418AsnGly: 3.418 ± 0.654
0.66AsnHis: 0.66 ± 0.222
1.739AsnIle: 1.739 ± 0.402
1.499AsnLys: 1.499 ± 0.305
2.758AsnLeu: 2.758 ± 0.489
1.079AsnMet: 1.079 ± 0.22
1.019AsnAsn: 1.019 ± 0.27
2.878AsnPro: 2.878 ± 0.478
0.959AsnGln: 0.959 ± 0.227
1.799AsnArg: 1.799 ± 0.338
1.739AsnSer: 1.739 ± 0.33
1.979AsnThr: 1.979 ± 0.43
2.159AsnVal: 2.159 ± 0.303
0.78AsnTrp: 0.78 ± 0.204
1.019AsnTyr: 1.019 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
4.677ProAla: 4.677 ± 0.423
0.42ProCys: 0.42 ± 0.213
3.418ProAsp: 3.418 ± 0.488
4.438ProGlu: 4.438 ± 0.809
1.859ProPhe: 1.859 ± 0.378
4.617ProGly: 4.617 ± 0.625
0.84ProHis: 0.84 ± 0.247
2.339ProIle: 2.339 ± 0.35
2.039ProLys: 2.039 ± 0.398
4.198ProLeu: 4.198 ± 0.504
0.959ProMet: 0.959 ± 0.256
2.219ProAsn: 2.219 ± 0.345
2.399ProPro: 2.399 ± 0.516
2.998ProGln: 2.998 ± 0.706
2.758ProArg: 2.758 ± 0.464
2.818ProSer: 2.818 ± 0.38
4.378ProThr: 4.378 ± 0.601
4.378ProVal: 4.378 ± 0.519
1.079ProTrp: 1.079 ± 0.423
1.559ProTyr: 1.559 ± 0.354
0.0ProXaa: 0.0 ± 0.0
Gln
4.138GlnAla: 4.138 ± 0.517
0.24GlnCys: 0.24 ± 0.114
1.499GlnAsp: 1.499 ± 0.309
2.219GlnGlu: 2.219 ± 0.377
1.439GlnPhe: 1.439 ± 0.348
4.737GlnGly: 4.737 ± 1.527
0.72GlnHis: 0.72 ± 0.207
1.859GlnIle: 1.859 ± 0.358
1.319GlnLys: 1.319 ± 0.33
3.358GlnLeu: 3.358 ± 0.765
1.199GlnMet: 1.199 ± 0.328
0.899GlnAsn: 0.899 ± 0.242
1.739GlnPro: 1.739 ± 0.339
1.679GlnGln: 1.679 ± 0.346
2.938GlnArg: 2.938 ± 0.429
0.959GlnSer: 0.959 ± 0.221
1.739GlnThr: 1.739 ± 0.314
2.579GlnVal: 2.579 ± 0.458
0.959GlnTrp: 0.959 ± 0.244
0.899GlnTyr: 0.899 ± 0.207
0.0GlnXaa: 0.0 ± 0.0
Arg
4.797ArgAla: 4.797 ± 0.722
1.199ArgCys: 1.199 ± 0.338
3.718ArgAsp: 3.718 ± 0.534
5.097ArgGlu: 5.097 ± 0.878
2.039ArgPhe: 2.039 ± 0.387
4.378ArgGly: 4.378 ± 0.635
1.739ArgHis: 1.739 ± 0.327
4.497ArgIle: 4.497 ± 0.471
2.998ArgLys: 2.998 ± 0.578
6.057ArgLeu: 6.057 ± 0.732
2.039ArgMet: 2.039 ± 0.349
2.339ArgAsn: 2.339 ± 0.359
3.178ArgPro: 3.178 ± 0.52
2.519ArgGln: 2.519 ± 0.348
5.937ArgArg: 5.937 ± 0.695
2.519ArgSer: 2.519 ± 0.351
2.219ArgThr: 2.219 ± 0.375
4.557ArgVal: 4.557 ± 0.477
1.679ArgTrp: 1.679 ± 0.366
2.579ArgTyr: 2.579 ± 0.473
0.0ArgXaa: 0.0 ± 0.0
Ser
4.078SerAla: 4.078 ± 0.539
0.12SerCys: 0.12 ± 0.09
3.058SerAsp: 3.058 ± 0.402
3.778SerGlu: 3.778 ± 0.563
2.099SerPhe: 2.099 ± 0.369
5.697SerGly: 5.697 ± 0.705
1.019SerHis: 1.019 ± 0.239
1.919SerIle: 1.919 ± 0.316
1.979SerLys: 1.979 ± 0.443
4.078SerLeu: 4.078 ± 0.561
1.199SerMet: 1.199 ± 0.273
1.499SerAsn: 1.499 ± 0.308
2.459SerPro: 2.459 ± 0.348
1.559SerGln: 1.559 ± 0.383
4.138SerArg: 4.138 ± 0.53
2.399SerSer: 2.399 ± 0.373
2.938SerThr: 2.938 ± 0.524
3.358SerVal: 3.358 ± 0.408
1.019SerTrp: 1.019 ± 0.268
1.259SerTyr: 1.259 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
5.817ThrAla: 5.817 ± 0.459
0.48ThrCys: 0.48 ± 0.155
3.538ThrAsp: 3.538 ± 0.595
4.617ThrGlu: 4.617 ± 0.517
2.219ThrPhe: 2.219 ± 0.407
6.117ThrGly: 6.117 ± 0.754
0.78ThrHis: 0.78 ± 0.233
2.459ThrIle: 2.459 ± 0.412
2.399ThrLys: 2.399 ± 0.386
5.457ThrLeu: 5.457 ± 0.735
1.319ThrMet: 1.319 ± 0.261
1.679ThrAsn: 1.679 ± 0.275
3.538ThrPro: 3.538 ± 0.463
2.219ThrGln: 2.219 ± 0.422
3.538ThrArg: 3.538 ± 0.539
3.178ThrSer: 3.178 ± 0.493
3.478ThrThr: 3.478 ± 0.506
4.977ThrVal: 4.977 ± 0.521
1.259ThrTrp: 1.259 ± 0.283
1.559ThrTyr: 1.559 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
6.596ValAla: 6.596 ± 0.638
0.959ValCys: 0.959 ± 0.249
5.157ValAsp: 5.157 ± 0.569
4.737ValGlu: 4.737 ± 0.461
2.039ValPhe: 2.039 ± 0.382
4.438ValGly: 4.438 ± 0.466
1.739ValHis: 1.739 ± 0.355
4.138ValIle: 4.138 ± 0.474
3.418ValLys: 3.418 ± 0.422
5.517ValLeu: 5.517 ± 0.64
1.619ValMet: 1.619 ± 0.368
2.698ValAsn: 2.698 ± 0.423
3.598ValPro: 3.598 ± 0.445
2.459ValGln: 2.459 ± 0.46
4.917ValArg: 4.917 ± 0.535
4.138ValSer: 4.138 ± 0.503
4.438ValThr: 4.438 ± 0.45
6.117ValVal: 6.117 ± 0.532
1.439ValTrp: 1.439 ± 0.329
2.159ValTyr: 2.159 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
1.799TrpAla: 1.799 ± 0.327
0.48TrpCys: 0.48 ± 0.187
1.619TrpAsp: 1.619 ± 0.361
1.439TrpGlu: 1.439 ± 0.266
0.6TrpPhe: 0.6 ± 0.156
1.679TrpGly: 1.679 ± 0.34
0.42TrpHis: 0.42 ± 0.186
1.139TrpIle: 1.139 ± 0.255
0.72TrpLys: 0.72 ± 0.181
1.319TrpLeu: 1.319 ± 0.289
0.6TrpMet: 0.6 ± 0.156
0.66TrpAsn: 0.66 ± 0.183
0.78TrpPro: 0.78 ± 0.212
0.78TrpGln: 0.78 ± 0.181
1.199TrpArg: 1.199 ± 0.259
1.199TrpSer: 1.199 ± 0.253
1.199TrpThr: 1.199 ± 0.31
1.379TrpVal: 1.379 ± 0.236
0.48TrpTrp: 0.48 ± 0.199
0.66TrpTyr: 0.66 ± 0.251
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.399TyrAla: 2.399 ± 0.387
0.18TyrCys: 0.18 ± 0.107
2.039TyrAsp: 2.039 ± 0.42
1.619TyrGlu: 1.619 ± 0.347
1.019TyrPhe: 1.019 ± 0.216
2.219TyrGly: 2.219 ± 0.347
0.48TyrHis: 0.48 ± 0.17
1.859TyrIle: 1.859 ± 0.441
1.079TyrLys: 1.079 ± 0.237
2.698TyrLeu: 2.698 ± 0.43
0.6TyrMet: 0.6 ± 0.17
1.379TyrAsn: 1.379 ± 0.302
1.619TyrPro: 1.619 ± 0.274
1.259TyrGln: 1.259 ± 0.261
2.219TyrArg: 2.219 ± 0.379
1.199TyrSer: 1.199 ± 0.293
1.739TyrThr: 1.739 ± 0.314
1.859TyrVal: 1.859 ± 0.366
0.3TyrTrp: 0.3 ± 0.14
1.199TyrTyr: 1.199 ± 0.283
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16677 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski