Geoglobus ahangari
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1973 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F7DBG7|A0A0F7DBG7_9EURY Uncharacterized protein OS=Geoglobus ahangari OX=113653 GN=GAH_01653 PE=4 SV=1
MM1 pKa = 7.79 AKK3 pKa = 9.79 YY4 pKa = 9.07 QCKK7 pKa = 9.09 VCGYY11 pKa = 10.22 VYY13 pKa = 10.75 DD14 pKa = 4.28 EE15 pKa = 5.8 AEE17 pKa = 4.39 GDD19 pKa = 3.82 PDD21 pKa = 3.67 NDD23 pKa = 3.28 IPAGTKK29 pKa = 9.36 WEE31 pKa = 4.7 DD32 pKa = 3.58 LPEE35 pKa = 4.73 DD36 pKa = 3.99 WVCPVCGASKK46 pKa = 10.89 EE47 pKa = 4.06 DD48 pKa = 3.82 FEE50 pKa = 6.28 KK51 pKa = 10.73 IEE53 pKa = 3.91
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.973
IPC_protein 3.872
Toseland 3.681
ProMoST 3.986
Dawson 3.859
Bjellqvist 4.088
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.605
Solomon 3.834
Lehninger 3.795
Nozaki 3.986
DTASelect 4.151
Thurlkill 3.745
EMBOSS 3.795
Sillero 3.999
Patrickios 0.769
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.931
Protein with the highest isoelectric point:
>tr|A0A0F7II16|A0A0F7II16_9EURY Uncharacterized protein OS=Geoglobus ahangari OX=113653 GN=GAH_00245 PE=4 SV=1
MM1 pKa = 7.48 SVIDD5 pKa = 3.6 LSGIEE10 pKa = 4.04 RR11 pKa = 11.84 ARR13 pKa = 11.84 RR14 pKa = 11.84 QYY16 pKa = 11.41 VDD18 pKa = 3.25 MILRR22 pKa = 11.84 AEE24 pKa = 5.33 FIRR27 pKa = 11.84 ADD29 pKa = 4.58 KK30 pKa = 10.68 IPKK33 pKa = 6.96 TCRR36 pKa = 11.84 RR37 pKa = 11.84 SKK39 pKa = 10.6 CPRR42 pKa = 11.84 DD43 pKa = 3.43 LRR45 pKa = 11.84 TT46 pKa = 3.47
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.428
IPC_protein 10.116
Toseland 10.599
ProMoST 10.262
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.833
Grimsley 10.716
Solomon 10.818
Lehninger 10.804
Nozaki 10.613
DTASelect 10.379
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.628
Patrickios 10.672
IPC_peptide 10.833
IPC2_peptide 9.648
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1973
0
1973
540133
39
1541
273.8
30.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.421 ± 0.059
0.978 ± 0.029
5.129 ± 0.042
8.964 ± 0.078
4.411 ± 0.043
7.444 ± 0.058
1.67 ± 0.025
7.187 ± 0.045
5.935 ± 0.061
9.311 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.653 ± 0.025
3.067 ± 0.04
3.793 ± 0.038
1.661 ± 0.026
6.701 ± 0.071
5.795 ± 0.051
4.18 ± 0.047
9.017 ± 0.052
0.965 ± 0.023
3.716 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here