Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12788 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B6HIF0|B6HIF0_PENRW Pc21g05930 protein OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=Pc21g05930 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 TQQLLPLCGLLLASVTIASKK22 pKa = 10.73 LDD24 pKa = 3.55 HH25 pKa = 7.68 DD26 pKa = 5.06 DD27 pKa = 3.5 VPNRR31 pKa = 11.84 CWPACGPVVGIANSCDD47 pKa = 3.12 ARR49 pKa = 11.84 HH50 pKa = 5.93 EE51 pKa = 4.22 RR52 pKa = 11.84 DD53 pKa = 3.38 SAEE56 pKa = 4.0 IQCICDD62 pKa = 2.94 WDD64 pKa = 3.85 AAKK67 pKa = 8.95 TQIPLCAACITQYY80 pKa = 9.23 QTDD83 pKa = 3.5 RR84 pKa = 11.84 RR85 pKa = 11.84 NNNTTRR91 pKa = 11.84 HH92 pKa = 5.9 DD93 pKa = 3.83 HH94 pKa = 7.79 DD95 pKa = 6.81 DD96 pKa = 5.18 DD97 pKa = 6.24 DD98 pKa = 5.94 DD99 pKa = 6.61 HH100 pKa = 9.43 DD101 pKa = 5.85 DD102 pKa = 5.18 DD103 pKa = 6.81 HH104 pKa = 9.36 DD105 pKa = 5.32 DD106 pKa = 5.16 DD107 pKa = 6.69 HH108 pKa = 9.19 DD109 pKa = 5.43 DD110 pKa = 3.63 DD111 pKa = 5.78 HH112 pKa = 9.6 DD113 pKa = 4.46 YY114 pKa = 11.71 DD115 pKa = 4.63 RR116 pKa = 11.84 DD117 pKa = 3.68 NDD119 pKa = 3.67 NDD121 pKa = 4.15 IDD123 pKa = 5.4 DD124 pKa = 5.56 DD125 pKa = 4.89 DD126 pKa = 6.11 NEE128 pKa = 4.39 ALDD131 pKa = 3.67 LVRR134 pKa = 11.84 YY135 pKa = 9.23 CKK137 pKa = 9.98 LTTTTYY143 pKa = 10.94 NSAAATTMGTSTSTAGANTATTTDD167 pKa = 3.76 PSNAATGTDD176 pKa = 3.38 SAGTGGTNSQDD187 pKa = 3.5 STDD190 pKa = 3.7 SAGASAGSASSPTPTPDD207 pKa = 2.9 AAAGILAPGAASMAGVMGIMALAWLL232 pKa = 4.4
Molecular weight: 24.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.821
IPC_protein 3.884
Toseland 3.63
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.91
Rodwell 3.706
Grimsley 3.541
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.38
Thurlkill 3.706
EMBOSS 3.91
Sillero 4.012
Patrickios 1.952
IPC_peptide 3.897
IPC2_peptide 3.986
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|B6GWN6|B6GWN6_PENRW Uncharacterized protein (Fragment) OS=Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) OX=500485 GN=Pc11g00010 PE=4 SV=1
AA1 pKa = 7.57 LRR3 pKa = 11.84 PTTVSRR9 pKa = 11.84 RR10 pKa = 11.84 IGSASTTVSRR20 pKa = 11.84 RR21 pKa = 11.84 TGSASTTVSRR31 pKa = 11.84 RR32 pKa = 11.84 IGSAISTVSRR42 pKa = 11.84 RR43 pKa = 11.84 IGSASTTVSRR53 pKa = 11.84 RR54 pKa = 11.84 TGSASTTVSRR64 pKa = 11.84 RR65 pKa = 11.84 IGSASTTVSRR75 pKa = 11.84 RR76 pKa = 11.84 IGSASTTVSRR86 pKa = 11.84 RR87 pKa = 11.84 TGSASTTVSRR97 pKa = 11.84 RR98 pKa = 11.84 TGSAA102 pKa = 2.77
Molecular weight: 10.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.502
IPC2_protein 11.477
IPC_protein 13.1
Toseland 13.261
ProMoST 13.759
Dawson 13.261
Bjellqvist 13.261
Wikipedia 13.729
Rodwell 12.749
Grimsley 13.29
Solomon 13.759
Lehninger 13.656
Nozaki 13.261
DTASelect 13.261
Thurlkill 13.261
EMBOSS 13.759
Sillero 13.261
Patrickios 12.486
IPC_peptide 13.759
IPC2_peptide 12.749
IPC2.peptide.svr19 9.378
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12788
0
12788
5708734
11
7287
446.4
49.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.27 ± 0.02
1.349 ± 0.009
5.599 ± 0.013
6.05 ± 0.02
3.763 ± 0.012
6.742 ± 0.021
2.489 ± 0.01
5.069 ± 0.015
4.614 ± 0.019
9.009 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.235 ± 0.009
3.697 ± 0.011
6.095 ± 0.024
4.02 ± 0.015
6.173 ± 0.02
8.414 ± 0.022
5.931 ± 0.013
6.136 ± 0.021
1.491 ± 0.009
2.823 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here