Amino acid dipepetide frequency for Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.084AlaAla: 8.084 ± 0.062	1.143AlaCys: 1.143 ± 0.014	4.123AlaAsp: 4.123 ± 0.03	4.983AlaGlu: 4.983 ± 0.041	3.098AlaPhe: 3.098 ± 0.026	5.555AlaGly: 5.555 ± 0.038	1.795AlaHis: 1.795 ± 0.019	4.25AlaIle: 4.25 ± 0.034	3.809AlaLys: 3.809 ± 0.027	7.575AlaLeu: 7.575 ± 0.041	1.967AlaMet: 1.967 ± 0.022	2.95AlaAsn: 2.95 ± 0.022	4.671AlaPro: 4.671 ± 0.041	3.279AlaGln: 3.279 ± 0.026	4.855AlaArg: 4.855 ± 0.036	7.052AlaSer: 7.052 ± 0.038	5.051AlaThr: 5.051 ± 0.031	5.205AlaVal: 5.205 ± 0.032	1.128AlaTrp: 1.128 ± 0.016	2.116AlaTyr: 2.116 ± 0.02	0.011AlaXaa: 0.011 ± 0.002
Cys	1.006CysAla: 1.006 ± 0.014	0.276CysCys: 0.276 ± 0.008	0.712CysAsp: 0.712 ± 0.013	0.649CysGlu: 0.649 ± 0.013	0.593CysPhe: 0.593 ± 0.011	1.011CysGly: 1.011 ± 0.015	0.389CysHis: 0.389 ± 0.01	0.756CysIle: 0.756 ± 0.013	0.535CysLys: 0.535 ± 0.011	1.399CysLeu: 1.399 ± 0.018	0.304CysMet: 0.304 ± 0.008	0.459CysAsn: 0.459 ± 0.009	0.734CysPro: 0.734 ± 0.014	0.523CysGln: 0.523 ± 0.01	0.883CysArg: 0.883 ± 0.015	1.036CysSer: 1.036 ± 0.017	0.742CysThr: 0.742 ± 0.012	0.87CysVal: 0.87 ± 0.014	0.228CysTrp: 0.228 ± 0.006	0.382CysTyr: 0.382 ± 0.008	0.002CysXaa: 0.002 ± 0.001
Asp	4.351AspAla: 4.351 ± 0.031	0.675AspCys: 0.675 ± 0.012	3.754AspAsp: 3.754 ± 0.036	4.153AspGlu: 4.153 ± 0.037	2.159AspPhe: 2.159 ± 0.022	3.808AspGly: 3.808 ± 0.028	1.363AspHis: 1.363 ± 0.015	3.207AspIle: 3.207 ± 0.025	2.247AspLys: 2.247 ± 0.022	5.193AspLeu: 5.193 ± 0.029	1.318AspMet: 1.318 ± 0.016	1.92AspAsn: 1.92 ± 0.019	3.371AspPro: 3.371 ± 0.025	2.002AspGln: 2.002 ± 0.019	3.156AspArg: 3.156 ± 0.03	4.22AspSer: 4.22 ± 0.029	3.051AspThr: 3.051 ± 0.025	3.537AspVal: 3.537 ± 0.03	0.897AspTrp: 0.897 ± 0.013	1.595AspTyr: 1.595 ± 0.019	0.012AspXaa: 0.012 ± 0.002
Glu	5.04GluAla: 5.04 ± 0.039	0.681GluCys: 0.681 ± 0.013	4.032GluAsp: 4.032 ± 0.033	5.029GluGlu: 5.029 ± 0.054	1.958GluPhe: 1.958 ± 0.019	3.608GluGly: 3.608 ± 0.027	1.418GluHis: 1.418 ± 0.019	3.152GluIle: 3.152 ± 0.025	3.464GluLys: 3.464 ± 0.031	5.072GluLeu: 5.072 ± 0.041	1.462GluMet: 1.462 ± 0.016	2.335GluAsn: 2.335 ± 0.023	2.853GluPro: 2.853 ± 0.04	2.424GluGln: 2.424 ± 0.026	3.83GluArg: 3.83 ± 0.032	4.47GluSer: 4.47 ± 0.03	3.61GluThr: 3.61 ± 0.03	3.436GluVal: 3.436 ± 0.027	0.908GluTrp: 0.908 ± 0.012	1.704GluTyr: 1.704 ± 0.017	0.011GluXaa: 0.011 ± 0.002
Phe	3.014PheAla: 3.014 ± 0.024	0.612PheCys: 0.612 ± 0.011	2.257PheAsp: 2.257 ± 0.02	2.144PheGlu: 2.144 ± 0.02	1.66PhePhe: 1.66 ± 0.018	2.824PheGly: 2.824 ± 0.028	0.956PheHis: 0.956 ± 0.014	1.915PheIle: 1.915 ± 0.021	1.464PheLys: 1.464 ± 0.02	3.534PheLeu: 3.534 ± 0.033	0.836PheMet: 0.836 ± 0.013	1.458PheAsn: 1.458 ± 0.02	2.08PhePro: 2.08 ± 0.019	1.458PheGln: 1.458 ± 0.016	2.023PheArg: 2.023 ± 0.018	3.029PheSer: 3.029 ± 0.024	2.127PheThr: 2.127 ± 0.021	2.432PheVal: 2.432 ± 0.025	0.644PheTrp: 0.644 ± 0.011	1.147PheTyr: 1.147 ± 0.016	0.016PheXaa: 0.016 ± 0.002
Gly	5.105GlyAla: 5.105 ± 0.039	0.966GlyCys: 0.966 ± 0.014	3.523GlyAsp: 3.523 ± 0.028	3.49GlyGlu: 3.49 ± 0.028	2.848GlyPhe: 2.848 ± 0.024	5.22GlyGly: 5.22 ± 0.042	1.741GlyHis: 1.741 ± 0.02	3.614GlyIle: 3.614 ± 0.029	3.255GlyLys: 3.255 ± 0.025	6.159GlyLeu: 6.159 ± 0.034	1.658GlyMet: 1.658 ± 0.02	2.504GlyAsn: 2.504 ± 0.023	3.404GlyPro: 3.404 ± 0.028	2.556GlyGln: 2.556 ± 0.022	4.054GlyArg: 4.054 ± 0.033	5.755GlySer: 5.755 ± 0.038	3.91GlyThr: 3.91 ± 0.029	4.32GlyVal: 4.32 ± 0.031	1.156GlyTrp: 1.156 ± 0.013	2.173GlyTyr: 2.173 ± 0.022	0.01GlyXaa: 0.01 ± 0.002
His	1.867HisAla: 1.867 ± 0.019	0.392HisCys: 0.392 ± 0.009	1.338HisAsp: 1.338 ± 0.018	1.381HisGlu: 1.381 ± 0.016	0.979HisPhe: 0.979 ± 0.014	1.794HisGly: 1.794 ± 0.019	0.834HisHis: 0.834 ± 0.013	1.296HisIle: 1.296 ± 0.017	0.885HisLys: 0.885 ± 0.013	2.371HisLeu: 2.371 ± 0.02	0.539HisMet: 0.539 ± 0.01	0.914HisAsn: 0.914 ± 0.014	1.723HisPro: 1.723 ± 0.02	1.031HisGln: 1.031 ± 0.014	1.662HisArg: 1.662 ± 0.018	1.961HisSer: 1.961 ± 0.023	1.351HisThr: 1.351 ± 0.015	1.482HisVal: 1.482 ± 0.017	0.377HisTrp: 0.377 ± 0.008	0.712HisTyr: 0.712 ± 0.013	0.005HisXaa: 0.005 ± 0.001
Ile	4.195IleAla: 4.195 ± 0.032	0.865IleCys: 0.865 ± 0.014	2.951IleAsp: 2.951 ± 0.025	2.924IleGlu: 2.924 ± 0.027	2.082IlePhe: 2.082 ± 0.02	3.337IleGly: 3.337 ± 0.028	1.308IleHis: 1.308 ± 0.015	2.591IleIle: 2.591 ± 0.026	2.13IleLys: 2.13 ± 0.024	4.709IleLeu: 4.709 ± 0.034	1.06IleMet: 1.06 ± 0.013	1.814IleAsn: 1.814 ± 0.02	3.308IlePro: 3.308 ± 0.026	2.004IleGln: 2.004 ± 0.02	2.914IleArg: 2.914 ± 0.023	4.12IleSer: 4.12 ± 0.03	2.838IleThr: 2.838 ± 0.025	3.229IleVal: 3.229 ± 0.029	0.759IleTrp: 0.759 ± 0.013	1.518IleTyr: 1.518 ± 0.019	0.035IleXaa: 0.035 ± 0.005
Lys	3.908LysAla: 3.908 ± 0.033	0.55LysCys: 0.55 ± 0.011	2.667LysAsp: 2.667 ± 0.023	3.142LysGlu: 3.142 ± 0.032	1.429LysPhe: 1.429 ± 0.017	2.834LysGly: 2.834 ± 0.023	1.088LysHis: 1.088 ± 0.014	2.237LysIle: 2.237 ± 0.021	2.969LysLys: 2.969 ± 0.037	3.891LysLeu: 3.891 ± 0.031	0.983LysMet: 0.983 ± 0.013	1.695LysAsn: 1.695 ± 0.021	2.649LysPro: 2.649 ± 0.027	1.82LysGln: 1.82 ± 0.021	3.277LysArg: 3.277 ± 0.027	3.437LysSer: 3.437 ± 0.026	2.719LysThr: 2.719 ± 0.023	2.747LysVal: 2.747 ± 0.023	0.691LysTrp: 0.691 ± 0.011	1.383LysTyr: 1.383 ± 0.018	0.024LysXaa: 0.024 ± 0.003
Leu	7.7LeuAla: 7.7 ± 0.043	1.331LeuCys: 1.331 ± 0.017	5.208LeuAsp: 5.208 ± 0.032	5.46LeuGlu: 5.46 ± 0.036	3.416LeuPhe: 3.416 ± 0.03	6.046LeuGly: 6.046 ± 0.036	2.338LeuHis: 2.338 ± 0.022	4.102LeuIle: 4.102 ± 0.034	4.01LeuLys: 4.01 ± 0.03	8.464LeuLeu: 8.464 ± 0.055	1.854LeuMet: 1.854 ± 0.019	3.249LeuAsn: 3.249 ± 0.028	5.527LeuPro: 5.527 ± 0.037	3.881LeuGln: 3.881 ± 0.028	5.86LeuArg: 5.86 ± 0.031	7.561LeuSer: 7.561 ± 0.043	4.783LeuThr: 4.783 ± 0.032	5.57LeuVal: 5.57 ± 0.041	1.247LeuTrp: 1.247 ± 0.016	2.44LeuTyr: 2.44 ± 0.02	0.045LeuXaa: 0.045 ± 0.006
Met	2.22MetAla: 2.22 ± 0.022	0.263MetCys: 0.263 ± 0.006	1.336MetAsp: 1.336 ± 0.017	1.356MetGlu: 1.356 ± 0.017	0.781MetPhe: 0.781 ± 0.012	1.534MetGly: 1.534 ± 0.02	0.537MetHis: 0.537 ± 0.011	1.053MetIle: 1.053 ± 0.013	1.01MetLys: 1.01 ± 0.014	1.872MetLeu: 1.872 ± 0.019	0.573MetMet: 0.573 ± 0.011	0.833MetAsn: 0.833 ± 0.014	1.286MetPro: 1.286 ± 0.017	0.912MetGln: 0.912 ± 0.013	1.278MetArg: 1.278 ± 0.015	1.964MetSer: 1.964 ± 0.018	1.352MetThr: 1.352 ± 0.015	1.378MetVal: 1.378 ± 0.015	0.28MetTrp: 0.28 ± 0.007	0.53MetTyr: 0.53 ± 0.009	0.002MetXaa: 0.002 ± 0.001
Asn	3.044AsnAla: 3.044 ± 0.021	0.491AsnCys: 0.491 ± 0.011	1.919AsnAsp: 1.919 ± 0.018	2.079AsnGlu: 2.079 ± 0.022	1.382AsnPhe: 1.382 ± 0.016	2.825AsnGly: 2.825 ± 0.027	0.887AsnHis: 0.887 ± 0.015	2.151AsnIle: 2.151 ± 0.023	1.526AsnLys: 1.526 ± 0.018	3.287AsnLeu: 3.287 ± 0.027	0.879AsnMet: 0.879 ± 0.013	1.449AsnAsn: 1.449 ± 0.018	2.598AsnPro: 2.598 ± 0.026	1.413AsnGln: 1.413 ± 0.017	2.04AsnArg: 2.04 ± 0.02	2.801AsnSer: 2.801 ± 0.022	2.21AsnThr: 2.21 ± 0.02	2.306AsnVal: 2.306 ± 0.023	0.578AsnTrp: 0.578 ± 0.011	1.093AsnTyr: 1.093 ± 0.015	0.016AsnXaa: 0.016 ± 0.002
Pro	5.177ProAla: 5.177 ± 0.046	0.621ProCys: 0.621 ± 0.012	3.263ProAsp: 3.263 ± 0.028	3.887ProGlu: 3.887 ± 0.036	2.122ProPhe: 2.122 ± 0.019	3.971ProGly: 3.971 ± 0.035	1.406ProHis: 1.406 ± 0.017	2.691ProIle: 2.691 ± 0.025	2.607ProLys: 2.607 ± 0.025	4.798ProLeu: 4.798 ± 0.03	1.169ProMet: 1.169 ± 0.015	2.248ProAsn: 2.248 ± 0.023	4.611ProPro: 4.611 ± 0.063	2.451ProGln: 2.451 ± 0.03	3.587ProArg: 3.587 ± 0.031	6.174ProSer: 6.174 ± 0.04	4.059ProThr: 4.059 ± 0.032	3.731ProVal: 3.731 ± 0.033	0.819ProTrp: 0.819 ± 0.011	1.546ProTyr: 1.546 ± 0.019	0.008ProXaa: 0.008 ± 0.001
Gln	3.323GlnAla: 3.323 ± 0.025	0.493GlnCys: 0.493 ± 0.009	2.03GlnAsp: 2.03 ± 0.02	2.369GlnGlu: 2.369 ± 0.023	1.357GlnPhe: 1.357 ± 0.015	2.465GlnGly: 2.465 ± 0.022	1.073GlnHis: 1.073 ± 0.015	1.975GlnIle: 1.975 ± 0.02	1.959GlnLys: 1.959 ± 0.023	3.477GlnLeu: 3.477 ± 0.024	0.929GlnMet: 0.929 ± 0.013	1.596GlnAsn: 1.596 ± 0.018	2.611GlnPro: 2.611 ± 0.032	2.127GlnGln: 2.127 ± 0.036	2.658GlnArg: 2.658 ± 0.024	3.337GlnSer: 3.337 ± 0.03	2.399GlnThr: 2.399 ± 0.023	2.223GlnVal: 2.223 ± 0.019	0.595GlnTrp: 0.595 ± 0.011	1.202GlnTyr: 1.202 ± 0.014	0.005GlnXaa: 0.005 ± 0.001
Arg	4.668ArgAla: 4.668 ± 0.032	0.785ArgCys: 0.785 ± 0.014	3.393ArgAsp: 3.393 ± 0.031	3.753ArgGlu: 3.753 ± 0.035	2.215ArgPhe: 2.215 ± 0.019	3.813ArgGly: 3.813 ± 0.032	1.624ArgHis: 1.624 ± 0.017	3.008ArgIle: 3.008 ± 0.024	3.335ArgLys: 3.335 ± 0.027	5.67ArgLeu: 5.67 ± 0.038	1.345ArgMet: 1.345 ± 0.015	2.303ArgAsn: 2.303 ± 0.023	3.578ArgPro: 3.578 ± 0.032	2.611ArgGln: 2.611 ± 0.025	5.047ArgArg: 5.047 ± 0.046	5.017ArgSer: 5.017 ± 0.038	3.313ArgThr: 3.313 ± 0.026	3.545ArgVal: 3.545 ± 0.025	0.97ArgTrp: 0.97 ± 0.011	1.716ArgTyr: 1.716 ± 0.019	0.025ArgXaa: 0.025 ± 0.004
Ser	6.642SerAla: 6.642 ± 0.033	1.007SerCys: 1.007 ± 0.016	4.384SerAsp: 4.384 ± 0.032	4.31SerGlu: 4.31 ± 0.033	3.103SerPhe: 3.103 ± 0.028	5.518SerGly: 5.518 ± 0.035	2.124SerHis: 2.124 ± 0.019	4.138SerIle: 4.138 ± 0.03	3.703SerLys: 3.703 ± 0.029	7.534SerLeu: 7.534 ± 0.047	1.835SerMet: 1.835 ± 0.018	3.071SerAsn: 3.071 ± 0.022	5.687SerPro: 5.687 ± 0.046	3.429SerGln: 3.429 ± 0.027	5.173SerArg: 5.173 ± 0.036	8.634SerSer: 8.634 ± 0.056	5.627SerThr: 5.627 ± 0.043	4.878SerVal: 4.878 ± 0.036	1.189SerTrp: 1.189 ± 0.016	2.134SerTyr: 2.134 ± 0.019	0.02SerXaa: 0.02 ± 0.003
Thr	4.971ThrAla: 4.971 ± 0.032	0.782ThrCys: 0.782 ± 0.014	2.88ThrAsp: 2.88 ± 0.024	3.276ThrGlu: 3.276 ± 0.03	2.183ThrPhe: 2.183 ± 0.02	4.214ThrGly: 4.214 ± 0.029	1.34ThrHis: 1.34 ± 0.02	3.13ThrIle: 3.13 ± 0.022	2.56ThrLys: 2.56 ± 0.023	5.263ThrLeu: 5.263 ± 0.034	1.224ThrMet: 1.224 ± 0.015	2.091ThrAsn: 2.091 ± 0.02	4.346ThrPro: 4.346 ± 0.037	2.152ThrGln: 2.152 ± 0.021	3.255ThrArg: 3.255 ± 0.028	5.32ThrSer: 5.32 ± 0.037	4.063ThrThr: 4.063 ± 0.039	3.773ThrVal: 3.773 ± 0.029	0.871ThrTrp: 0.871 ± 0.012	1.601ThrTyr: 1.601 ± 0.018	0.011ThrXaa: 0.011 ± 0.002
Val	5.098ValAla: 5.098 ± 0.034	0.909ValCys: 0.909 ± 0.014	3.754ValAsp: 3.754 ± 0.029	3.708ValGlu: 3.708 ± 0.028	2.524ValPhe: 2.524 ± 0.023	3.99ValGly: 3.99 ± 0.027	1.486ValHis: 1.486 ± 0.019	3.081ValIle: 3.081 ± 0.026	2.697ValLys: 2.697 ± 0.028	5.635ValLeu: 5.635 ± 0.035	1.363ValMet: 1.363 ± 0.017	2.289ValAsn: 2.289 ± 0.022	3.665ValPro: 3.665 ± 0.028	2.424ValGln: 2.424 ± 0.021	3.443ValArg: 3.443 ± 0.025	4.934ValSer: 4.934 ± 0.034	3.518ValThr: 3.518 ± 0.028	4.172ValVal: 4.172 ± 0.034	0.851ValTrp: 0.851 ± 0.014	1.802ValTyr: 1.802 ± 0.017	0.018ValXaa: 0.018 ± 0.003
Trp	1.153TrpAla: 1.153 ± 0.014	0.204TrpCys: 0.204 ± 0.006	0.87TrpAsp: 0.87 ± 0.013	0.872TrpGlu: 0.872 ± 0.015	0.53TrpPhe: 0.53 ± 0.009	0.941TrpGly: 0.941 ± 0.014	0.377TrpHis: 0.377 ± 0.008	0.799TrpIle: 0.799 ± 0.013	0.828TrpLys: 0.828 ± 0.012	1.418TrpLeu: 1.418 ± 0.019	0.398TrpMet: 0.398 ± 0.008	0.652TrpAsn: 0.652 ± 0.01	0.634TrpPro: 0.634 ± 0.01	0.568TrpGln: 0.568 ± 0.011	0.985TrpArg: 0.985 ± 0.017	1.128TrpSer: 1.128 ± 0.013	0.936TrpThr: 0.936 ± 0.013	0.894TrpVal: 0.894 ± 0.014	0.283TrpTrp: 0.283 ± 0.008	0.44TrpTyr: 0.44 ± 0.009	0.001TrpXaa: 0.001 ± 0.0
Tyr	2.115TyrAla: 2.115 ± 0.019	0.44TyrCys: 0.44 ± 0.009	1.582TyrAsp: 1.582 ± 0.02	1.513TyrGlu: 1.513 ± 0.017	1.201TyrPhe: 1.201 ± 0.016	2.097TyrGly: 2.097 ± 0.021	0.806TyrHis: 0.806 ± 0.012	1.521TyrIle: 1.521 ± 0.02	1.12TyrLys: 1.12 ± 0.015	2.732TyrLeu: 2.732 ± 0.026	0.65TyrMet: 0.65 ± 0.011	1.137TyrAsn: 1.137 ± 0.014	1.599TyrPro: 1.599 ± 0.018	1.131TyrGln: 1.131 ± 0.014	1.733TyrArg: 1.733 ± 0.018	2.171TyrSer: 2.171 ± 0.022	1.639TyrThr: 1.639 ± 0.017	1.61TyrVal: 1.61 ± 0.017	0.443TyrTrp: 0.443 ± 0.009	0.974TyrTyr: 0.974 ± 0.016	0.018TyrXaa: 0.018 ± 0.003
Xaa	0.015XaaAla: 0.015 ± 0.002	0.004XaaCys: 0.004 ± 0.001	0.014XaaAsp: 0.014 ± 0.002	0.018XaaGlu: 0.018 ± 0.002	0.013XaaPhe: 0.013 ± 0.002	0.014XaaGly: 0.014 ± 0.002	0.004XaaHis: 0.004 ± 0.001	0.021XaaIle: 0.021 ± 0.003	0.023XaaLys: 0.023 ± 0.003	0.037XaaLeu: 0.037 ± 0.005	0.005XaaMet: 0.005 ± 0.001	0.008XaaAsn: 0.008 ± 0.001	0.017XaaPro: 0.017 ± 0.002	0.004XaaGln: 0.004 ± 0.001	0.02XaaArg: 0.02 ± 0.003	0.021XaaSer: 0.021 ± 0.003	0.011XaaThr: 0.011 ± 0.002	0.022XaaVal: 0.022 ± 0.003	0.002XaaTrp: 0.002 ± 0.001	0.021XaaTyr: 0.021 ± 0.003	0.015XaaXaa: 0.015 ± 0.003

Statistics based on 12788 proteins (5708734 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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