Reovirus GCRV104
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H9XH60|H9XH60_9REOV Uncharacterized protein OS=Reovirus GCRV104 OX=1164802 PE=4 SV=1
MM1 pKa = 7.58 GNAQTSVTNVTVNGDD16 pKa = 3.67 GNVIRR21 pKa = 11.84 PSAEE25 pKa = 3.84 FQATAAPSFDD35 pKa = 4.76 LNPGVLNPNGNLYY48 pKa = 10.0 IEE50 pKa = 4.47 QPDD53 pKa = 3.18 MDD55 pKa = 4.22 ATVTAQSLVLLHH67 pKa = 7.35 DD68 pKa = 4.1 KK69 pKa = 10.36 TGSPYY74 pKa = 10.91 AYY76 pKa = 10.11 VADD79 pKa = 4.11 NTRR82 pKa = 11.84 EE83 pKa = 4.05 TLDD86 pKa = 4.08 KK87 pKa = 10.42 IVNYY91 pKa = 9.58 IANFQPLLEE100 pKa = 4.72 ASGLTCPPRR109 pKa = 11.84 MISFYY114 pKa = 9.61 CEE116 pKa = 3.47 RR117 pKa = 11.84 MSGYY121 pKa = 10.64 VGTSAITAINNPVEE135 pKa = 4.67 DD136 pKa = 4.67 GEE138 pKa = 5.23 FITQAKK144 pKa = 7.69 MAQFRR149 pKa = 11.84 KK150 pKa = 9.82 QLGLFYY156 pKa = 11.02 DD157 pKa = 4.2 KK158 pKa = 11.46 VKK160 pKa = 10.63 DD161 pKa = 3.28 WHH163 pKa = 7.01 VDD165 pKa = 3.26 LKK167 pKa = 10.26 MAQAMLPLNVPTGTYY182 pKa = 8.78 QLEE185 pKa = 4.24 FTSYY189 pKa = 10.21 RR190 pKa = 11.84 DD191 pKa = 3.44 FMTEE195 pKa = 3.87 YY196 pKa = 10.4 LPEE199 pKa = 4.45 SNICKK204 pKa = 10.24 LYY206 pKa = 9.86 PDD208 pKa = 4.03 EE209 pKa = 4.56 ALEE212 pKa = 4.2 VAANLIPAIRR222 pKa = 11.84 TGLTKK227 pKa = 10.39 EE228 pKa = 3.78 ISDD231 pKa = 3.95 VNSTGLIAGAIMAPQEE247 pKa = 4.52 GGGSLTDD254 pKa = 3.89 GSNTGTSLLQDD265 pKa = 3.81 VASMNANVITASSTIPVSSLNVIPEE290 pKa = 4.29 PKK292 pKa = 10.06 SFSYY296 pKa = 11.02 DD297 pKa = 2.9 SLAPDD302 pKa = 3.31 EE303 pKa = 5.11 VYY305 pKa = 10.21 WIQQVPSVQVRR316 pKa = 11.84 FSVDD320 pKa = 3.11 VEE322 pKa = 4.33 TVTYY326 pKa = 11.11 YY327 pKa = 10.24 MGFEE331 pKa = 4.03 PAQVNTVIDD340 pKa = 3.91 LRR342 pKa = 11.84 EE343 pKa = 4.12 CSSASFVFDD352 pKa = 3.2 LTGTQFAADD361 pKa = 4.06 YY362 pKa = 7.19 PTSKK366 pKa = 10.6 LGLLMIRR373 pKa = 11.84 LTDD376 pKa = 3.93 SITAVTPSTEE386 pKa = 3.28 IKK388 pKa = 10.94 ANVLEE393 pKa = 4.77 ISDD396 pKa = 3.54 ATIQQRR402 pKa = 11.84 SRR404 pKa = 11.84 KK405 pKa = 8.6 AVSGLSQLATTVLTYY420 pKa = 9.89 TYY422 pKa = 10.18 EE423 pKa = 4.65 RR424 pKa = 11.84 EE425 pKa = 4.1 VLPNDD430 pKa = 3.37 DD431 pKa = 3.51 AYY433 pKa = 11.54 YY434 pKa = 10.75 YY435 pKa = 10.29 LVPCTMQPTAYY446 pKa = 8.31 TALNYY451 pKa = 8.49 PCSVGGTLTVTPIDD465 pKa = 3.73 NTEE468 pKa = 3.91 VTVNGIMVDD477 pKa = 3.76 SIIPSEE483 pKa = 4.59 LIGSQTEE490 pKa = 4.24 EE491 pKa = 3.9 EE492 pKa = 4.93 QMTCLPNEE500 pKa = 3.86 AAEE503 pKa = 4.08 LLKK506 pKa = 10.97 HH507 pKa = 6.0 KK508 pKa = 10.48 VQICAGTIAKK518 pKa = 8.36 MDD520 pKa = 4.05 PSGVSEE526 pKa = 4.5 DD527 pKa = 3.99 TPAVAAVAGILALQNEE543 pKa = 4.52 TRR545 pKa = 11.84 AVRR548 pKa = 11.84 LDD550 pKa = 3.23 IKK552 pKa = 11.09 SLFSKK557 pKa = 10.43 ARR559 pKa = 11.84 RR560 pKa = 11.84 AINMLVGRR568 pKa = 11.84 PSTVLGLAKK577 pKa = 10.08 PVISDD582 pKa = 3.72 PNVWMQIATGVAEE595 pKa = 4.73 AVASGNVNSAMRR607 pKa = 11.84 DD608 pKa = 3.37 SARR611 pKa = 11.84 RR612 pKa = 11.84 LAALRR617 pKa = 11.84 SVSGAKK623 pKa = 9.96 GKK625 pKa = 10.11 LLSLVAQRR633 pKa = 11.84 FPPNPP638 pKa = 3.43
Molecular weight: 68.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.559
IPC2_protein 4.609
IPC_protein 4.558
Toseland 4.393
ProMoST 4.673
Dawson 4.507
Bjellqvist 4.66
Wikipedia 4.38
Rodwell 4.393
Grimsley 4.304
Solomon 4.507
Lehninger 4.469
Nozaki 4.609
DTASelect 4.787
Thurlkill 4.406
EMBOSS 4.406
Sillero 4.673
Patrickios 3.617
IPC_peptide 4.52
IPC2_peptide 4.66
IPC2.peptide.svr19 4.627
Protein with the highest isoelectric point:
>tr|H9XH56|H9XH56_9REOV VP3 OS=Reovirus GCRV104 OX=1164802 PE=4 SV=1
MM1 pKa = 8.1 DD2 pKa = 3.92 NHH4 pKa = 7.44 DD5 pKa = 4.26 DD6 pKa = 3.89 LAVEE10 pKa = 4.26 RR11 pKa = 11.84 LINCLTGEE19 pKa = 4.86 LEE21 pKa = 4.14 LTDD24 pKa = 6.48 DD25 pKa = 4.26 IFLAANSALRR35 pKa = 11.84 SRR37 pKa = 11.84 DD38 pKa = 2.74 RR39 pKa = 11.84 SYY41 pKa = 11.77 VYY43 pKa = 10.28 RR44 pKa = 11.84 QLNAIPFHH52 pKa = 5.85 TPVYY56 pKa = 7.53 VTRR59 pKa = 11.84 AILVNRR65 pKa = 11.84 DD66 pKa = 2.69 HH67 pKa = 7.57 RR68 pKa = 11.84 DD69 pKa = 3.35 YY70 pKa = 11.76 YY71 pKa = 11.11 HH72 pKa = 6.8 IDD74 pKa = 2.89 SRR76 pKa = 11.84 GLVRR80 pKa = 11.84 RR81 pKa = 11.84 KK82 pKa = 10.39 LSVTVGADD90 pKa = 3.1 DD91 pKa = 4.62 DD92 pKa = 5.66 VYY94 pKa = 11.65 VPCCNIGPLLAPHH107 pKa = 6.52 RR108 pKa = 11.84 TLEE111 pKa = 4.57 GYY113 pKa = 10.66 GILDD117 pKa = 3.63 PKK119 pKa = 10.48 FAATIKK125 pKa = 10.83 DD126 pKa = 3.5 PASRR130 pKa = 11.84 IAITFAKK137 pKa = 10.03 SATVQARR144 pKa = 11.84 QVKK147 pKa = 10.42 DD148 pKa = 3.87 DD149 pKa = 3.33 ITRR152 pKa = 11.84 FLQALLLMNGKK163 pKa = 8.61 QAIVGDD169 pKa = 4.58 EE170 pKa = 4.09 YY171 pKa = 11.66 ADD173 pKa = 4.12 RR174 pKa = 11.84 SPTPPCEE181 pKa = 4.03 TCPALHH187 pKa = 6.69 AVSQVIQLLGFAPPNLSPFLALPAGGLWHH216 pKa = 6.58 MPGTAKK222 pKa = 10.3 VMPVQVPQLLNDD234 pKa = 4.5 LVNLAILRR242 pKa = 11.84 VMLRR246 pKa = 11.84 IPDD249 pKa = 3.56 EE250 pKa = 3.82 LTYY253 pKa = 10.85 FGVRR257 pKa = 11.84 VLLAAAYY264 pKa = 9.9 SEE266 pKa = 4.61 SYY268 pKa = 10.01 SWAVLQCKK276 pKa = 9.97 HH277 pKa = 6.33 FFPDD281 pKa = 3.98 LSVHH285 pKa = 6.89 AMYY288 pKa = 8.45 KK289 pKa = 9.18 TNFSGGYY296 pKa = 10.03 APIFDD301 pKa = 4.19 WPSPRR306 pKa = 11.84 SDD308 pKa = 3.27 YY309 pKa = 11.09 RR310 pKa = 11.84 FTYY313 pKa = 10.02 LGHH316 pKa = 6.27 RR317 pKa = 11.84 TMTSEE322 pKa = 4.7 DD323 pKa = 4.06 KK324 pKa = 10.81 PVLPLDD330 pKa = 3.79 LKK332 pKa = 11.15 KK333 pKa = 10.64 VDD335 pKa = 3.22 EE336 pKa = 4.4 TARR339 pKa = 11.84 KK340 pKa = 9.13 FDD342 pKa = 3.88 LVDD345 pKa = 3.02 VAEE348 pKa = 4.16 RR349 pKa = 11.84 MKK351 pKa = 10.72 TYY353 pKa = 10.47 LADD356 pKa = 3.83 INTHH360 pKa = 4.57 THH362 pKa = 6.05 ASMKK366 pKa = 9.43 YY367 pKa = 8.07 AHH369 pKa = 6.95 HH370 pKa = 6.68 VMALTSSSFTVQTATEE386 pKa = 4.21 SVYY389 pKa = 11.18 AEE391 pKa = 4.03 YY392 pKa = 9.17 TQNPVIEE399 pKa = 4.31 QPIPPDD405 pKa = 2.96 SWTGPIGYY413 pKa = 9.28 LRR415 pKa = 11.84 LLKK418 pKa = 9.9 TDD420 pKa = 3.69 APAPALSLHH429 pKa = 5.03 RR430 pKa = 11.84 TWRR433 pKa = 11.84 NAAVAVAKK441 pKa = 10.46 DD442 pKa = 3.8 MRR444 pKa = 11.84 QMDD447 pKa = 4.72 PLQQAIMRR455 pKa = 11.84 TQYY458 pKa = 8.06 VTPRR462 pKa = 11.84 GGSGYY467 pKa = 8.58 SLRR470 pKa = 11.84 TSLEE474 pKa = 3.77 AANVAIRR481 pKa = 11.84 DD482 pKa = 3.72 FRR484 pKa = 11.84 NAGVKK489 pKa = 9.94 ISTKK493 pKa = 9.85 IYY495 pKa = 10.13 QAAQTAQIPFEE506 pKa = 4.18 VLLPAVLAAVTLGTRR521 pKa = 11.84 FQVQRR526 pKa = 11.84 RR527 pKa = 11.84 PRR529 pKa = 11.84 AIMPLNIVQQTVSAIHH545 pKa = 6.43 TIVADD550 pKa = 4.06 YY551 pKa = 10.82 INKK554 pKa = 9.24 HH555 pKa = 5.61 MNLSTTSGSAVQEE568 pKa = 3.98 KK569 pKa = 10.32 VIPLVLFASTTPTTVVNVDD588 pKa = 3.13 VKK590 pKa = 11.4 ACDD593 pKa = 3.21 ACVTYY598 pKa = 10.55 SYY600 pKa = 10.56 FLSVICAAMYY610 pKa = 9.84 EE611 pKa = 4.37 GLNPHH616 pKa = 6.83 GDD618 pKa = 3.6 PRR620 pKa = 11.84 PFMGVPVLPYY630 pKa = 8.95 TNRR633 pKa = 11.84 VSSAMMTDD641 pKa = 3.64 EE642 pKa = 4.36 ASGMQVMVQQLARR655 pKa = 11.84 LYY657 pKa = 10.14 QNGFRR662 pKa = 11.84 YY663 pKa = 8.75 MVSDD667 pKa = 3.79 PMAPGNIFEE676 pKa = 5.38 LPTLTFPSGSTATSTEE692 pKa = 3.82 HH693 pKa = 5.93 TANNSTMMDD702 pKa = 3.43 HH703 pKa = 7.83 FITTWLPEE711 pKa = 3.92 HH712 pKa = 5.72 TTNPEE717 pKa = 3.25 LKK719 pKa = 10.48 AIVRR723 pKa = 11.84 QMSIRR728 pKa = 11.84 DD729 pKa = 3.62 NYY731 pKa = 9.3 VCQGDD736 pKa = 4.29 DD737 pKa = 3.7 GMCIIDD743 pKa = 3.74 NVSGRR748 pKa = 11.84 RR749 pKa = 11.84 ISAEE753 pKa = 3.77 SLTEE757 pKa = 4.02 FTTLLNKK764 pKa = 10.29 YY765 pKa = 8.54 GRR767 pKa = 11.84 QFGWVYY773 pKa = 10.78 DD774 pKa = 3.88 IDD776 pKa = 4.28 ASGCAEE782 pKa = 4.03 YY783 pKa = 11.05 LKK785 pKa = 10.32 IYY787 pKa = 9.85 AVNGCRR793 pKa = 11.84 VPNCSRR799 pKa = 11.84 HH800 pKa = 5.82 PILGKK805 pKa = 10.68 EE806 pKa = 4.13 MANPDD811 pKa = 4.73 AYY813 pKa = 10.79 DD814 pKa = 3.64 PWPQIINALMGLWRR828 pKa = 11.84 SGLTDD833 pKa = 4.36 AYY835 pKa = 10.51 DD836 pKa = 3.7 TVDD839 pKa = 2.71 WTRR842 pKa = 11.84 YY843 pKa = 5.66 MWALVTIYY851 pKa = 10.77 ASGSMTTTKK860 pKa = 10.52 GQRR863 pKa = 11.84 LRR865 pKa = 11.84 LAYY868 pKa = 9.74 PMSTFITLGIPPIRR882 pKa = 11.84 IWGCDD887 pKa = 3.2 PFTVSVYY894 pKa = 9.64 MPPGDD899 pKa = 3.43 MGVYY903 pKa = 10.69 ALINVLRR910 pKa = 11.84 HH911 pKa = 5.74 HH912 pKa = 7.63 LYY914 pKa = 8.02 TTIAAHH920 pKa = 6.01 GDD922 pKa = 3.2 KK923 pKa = 10.56 YY924 pKa = 10.98 RR925 pKa = 11.84 QDD927 pKa = 3.77 RR928 pKa = 11.84 GDD930 pKa = 4.22 DD931 pKa = 3.36 MFDD934 pKa = 3.13 GHH936 pKa = 6.68 NVLQFLHH943 pKa = 6.48 DD944 pKa = 3.42 TRR946 pKa = 11.84 FTHH949 pKa = 7.39 GYY951 pKa = 8.47 FAAAHH956 pKa = 5.87 HH957 pKa = 6.01 RR958 pKa = 11.84 VPLRR962 pKa = 11.84 EE963 pKa = 3.99 SRR965 pKa = 11.84 EE966 pKa = 3.76 VDD968 pKa = 2.9 AKK970 pKa = 11.21 ARR972 pKa = 11.84 LDD974 pKa = 3.88 MISALSDD981 pKa = 3.36 FLYY984 pKa = 10.56 HH985 pKa = 7.56 DD986 pKa = 3.87 PEE988 pKa = 4.13 LRR990 pKa = 11.84 FRR992 pKa = 11.84 VIAGRR997 pKa = 11.84 RR998 pKa = 11.84 LWDD1001 pKa = 3.35 TYY1003 pKa = 11.63 GQGQHH1008 pKa = 5.68 GAYY1011 pKa = 9.67 LHH1013 pKa = 6.49 RR1014 pKa = 11.84 VPSLDD1019 pKa = 3.64 DD1020 pKa = 3.57 VPHH1023 pKa = 6.35 RR1024 pKa = 11.84 WYY1026 pKa = 10.62 EE1027 pKa = 3.84 EE1028 pKa = 3.48 ARR1030 pKa = 11.84 EE1031 pKa = 3.95 ADD1033 pKa = 3.85 RR1034 pKa = 11.84 ATLSDD1039 pKa = 3.71 EE1040 pKa = 3.8 EE1041 pKa = 4.6 RR1042 pKa = 11.84 LFKK1045 pKa = 10.7 SLHH1048 pKa = 5.51 RR1049 pKa = 11.84 AITRR1053 pKa = 11.84 PSLMLSKK1060 pKa = 11.06 LLMAYY1065 pKa = 10.22 LRR1067 pKa = 11.84 VRR1069 pKa = 11.84 WDD1071 pKa = 2.87 IGEE1074 pKa = 4.38 LMPAAVSPQVPLTAGMHH1091 pKa = 5.46 PQNPDD1096 pKa = 2.52 MFMKK1100 pKa = 8.25 MTSLGPFLEE1109 pKa = 4.4 RR1110 pKa = 11.84 VGQYY1114 pKa = 9.69 FHH1116 pKa = 7.8 DD1117 pKa = 3.94 ALFVKK1122 pKa = 10.21 RR1123 pKa = 11.84 VVSGMDD1129 pKa = 3.39 VNTLDD1134 pKa = 4.34 AALLKK1139 pKa = 10.69 LRR1141 pKa = 11.84 ILGAPANALVGVIMQSGFSEE1161 pKa = 4.44 SEE1163 pKa = 3.72 AAEE1166 pKa = 3.76 IAGRR1170 pKa = 11.84 IQLKK1174 pKa = 9.73 DD1175 pKa = 3.15 ARR1177 pKa = 11.84 TVQIARR1183 pKa = 11.84 VVNLSVPDD1191 pKa = 3.17 TWMLFNFHH1199 pKa = 7.05 LLLHH1203 pKa = 6.1 ATVNPHH1209 pKa = 5.61 GMYY1212 pKa = 10.67 GQTTFTKK1219 pKa = 9.97 IPPSMPWLRR1228 pKa = 11.84 SILLFLGSSINMTRR1242 pKa = 11.84 VGPIHH1247 pKa = 5.87 QVYY1250 pKa = 9.62 LARR1253 pKa = 11.84 IHH1255 pKa = 5.84 GTGASLARR1263 pKa = 11.84 QFQQWMSSAHH1273 pKa = 6.04 KK1274 pKa = 10.49
Molecular weight: 142.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.109
IPC2_protein 7.439
IPC_protein 7.293
Toseland 7.19
ProMoST 7.922
Dawson 7.951
Bjellqvist 8.302
Wikipedia 7.892
Rodwell 7.966
Grimsley 7.322
Solomon 7.98
Lehninger 7.995
Nozaki 8.39
DTASelect 8.112
Thurlkill 8.141
EMBOSS 8.185
Sillero 8.404
Patrickios 4.139
IPC_peptide 7.98
IPC2_peptide 7.512
IPC2.peptide.svr19 7.354
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
7523
140
1294
683.9
75.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.305 ± 0.299
1.196 ± 0.16
5.516 ± 0.14
3.722 ± 0.282
3.27 ± 0.265
5.37 ± 0.326
2.353 ± 0.334
5.251 ± 0.288
2.459 ± 0.353
9.451 ± 0.278
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.523 ± 0.176
4.161 ± 0.369
6.886 ± 0.352
4.373 ± 0.215
5.543 ± 0.304
7.298 ± 0.529
7.563 ± 0.264
7.922 ± 0.196
1.276 ± 0.141
3.562 ± 0.308
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here