Thiotrichales bacterium HS_08

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; unclassified Thiotrichales

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4918 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6FC32|A0A1H6FC32_9GAMM Abequosyltransferase RfbV OS=Thiotrichales bacterium HS_08 OX=1899563 GN=rfbV PE=4 SV=1
MM1 pKa = 7.87KK2 pKa = 10.23IPITMRR8 pKa = 11.84QLLLFGSCMTLAGWMNTASADD29 pKa = 3.56VCVPTDD35 pKa = 3.66PNYY38 pKa = 11.14NPDD41 pKa = 5.16DD42 pKa = 5.16PICLTNPPPCDD53 pKa = 3.45PNVNPACQDD62 pKa = 3.31PVEE65 pKa = 5.03PPACDD70 pKa = 3.55PNVNPACQDD79 pKa = 3.31PVEE82 pKa = 5.03PPACDD87 pKa = 3.55PNVNPACQDD96 pKa = 3.31PVEE99 pKa = 5.03PPACDD104 pKa = 3.55PNVNPACQDD113 pKa = 3.31PVEE116 pKa = 5.03PPACDD121 pKa = 3.55PNVNPACQDD130 pKa = 3.26PVDD133 pKa = 4.55PQACDD138 pKa = 3.67PNVDD142 pKa = 4.16PACQDD147 pKa = 3.32SVDD150 pKa = 4.48PAACDD155 pKa = 3.59PNVNPGCTTNTPPPIDD171 pKa = 4.07CSLLINKK178 pKa = 8.57DD179 pKa = 3.33MPEE182 pKa = 4.24CTGEE186 pKa = 4.05QPKK189 pKa = 10.26FCMDD193 pKa = 3.14APEE196 pKa = 5.09RR197 pKa = 11.84GLFCDD202 pKa = 3.58EE203 pKa = 5.14FGHH206 pKa = 6.03KK207 pKa = 9.33VCRR210 pKa = 11.84VDD212 pKa = 5.11LIRR215 pKa = 11.84PGFLSCEE222 pKa = 4.18DD223 pKa = 3.56VPGVCVEE230 pKa = 4.77DD231 pKa = 3.78PSKK234 pKa = 11.4NLFCDD239 pKa = 3.3EE240 pKa = 4.95RR241 pKa = 11.84GKK243 pKa = 9.46QLCFPDD249 pKa = 3.87EE250 pKa = 4.19EE251 pKa = 5.18LGISCGDD258 pKa = 3.64VQCEE262 pKa = 3.97EE263 pKa = 4.45NPDD266 pKa = 3.25EE267 pKa = 6.34GIYY270 pKa = 10.58CDD272 pKa = 4.45AKK274 pKa = 10.47GHH276 pKa = 6.55PICEE280 pKa = 4.11EE281 pKa = 4.03NPLKK285 pKa = 10.9GIFCHH290 pKa = 6.91EE291 pKa = 4.32IPPACIHH298 pKa = 7.15DD299 pKa = 4.64PDD301 pKa = 5.03NGMTCDD307 pKa = 3.6ANHH310 pKa = 6.14NQGFVCIDD318 pKa = 3.84DD319 pKa = 4.38NDD321 pKa = 4.19PNNNVSCDD329 pKa = 3.62DD330 pKa = 3.56QGRR333 pKa = 11.84LCVTIPSGEE342 pKa = 4.37TFCEE346 pKa = 3.78EE347 pKa = 5.29DD348 pKa = 3.91GLFDD352 pKa = 4.01PAQFNSPDD360 pKa = 4.43LIVDD364 pKa = 3.92PALFNFFDD372 pKa = 4.54EE373 pKa = 4.5EE374 pKa = 5.91DD375 pKa = 3.55ITQLPPEE382 pKa = 4.42FFIGITPEE390 pKa = 3.74QFDD393 pKa = 3.94EE394 pKa = 4.65FRR396 pKa = 11.84PDD398 pKa = 3.57AYY400 pKa = 10.76GHH402 pKa = 6.68LNSGQVQALPLDD414 pKa = 3.66VLMDD418 pKa = 3.44MDD420 pKa = 3.85GQEE423 pKa = 4.75FFNFPPDD430 pKa = 3.39VFDD433 pKa = 6.53DD434 pKa = 4.91FSQEE438 pKa = 4.0QIGALPPKK446 pKa = 9.76ALRR449 pKa = 11.84GLTPTQFNRR458 pKa = 11.84LNPDD462 pKa = 3.06ALQGLDD468 pKa = 3.11RR469 pKa = 11.84HH470 pKa = 6.86DD471 pKa = 3.76IAALPPEE478 pKa = 4.59LWMDD482 pKa = 3.66IQQDD486 pKa = 4.09VFFGLPDD493 pKa = 3.89DD494 pKa = 5.14AFQGMDD500 pKa = 4.65HH501 pKa = 7.13EE502 pKa = 5.13DD503 pKa = 4.13CEE505 pKa = 4.35NLPPLMFGQMDD516 pKa = 3.83AAKK519 pKa = 9.98VGNLGGDD526 pKa = 3.96CLMGMDD532 pKa = 5.1SEE534 pKa = 5.52QFMQLPDD541 pKa = 3.39AALAGLGADD550 pKa = 4.43HH551 pKa = 7.01LHH553 pKa = 6.37GLPPRR558 pKa = 11.84VFNDD562 pKa = 3.18WTPEE566 pKa = 3.96RR567 pKa = 11.84FDD569 pKa = 4.59ALPDD573 pKa = 3.54AALTQLAQEE582 pKa = 4.85NPFEE586 pKa = 4.05MLEE589 pKa = 3.89ILANLDD595 pKa = 3.43TIAADD600 pKa = 3.98GVSADD605 pKa = 4.42DD606 pKa = 3.77LALVEE611 pKa = 5.23GFLPPGVSIDD621 pKa = 3.89PNTGEE626 pKa = 5.26LLFTGNEE633 pKa = 3.86FDD635 pKa = 4.12GQRR638 pKa = 11.84LPMPILEE645 pKa = 4.61FGADD649 pKa = 3.51VFGSDD654 pKa = 2.8IDD656 pKa = 4.5FRR658 pKa = 11.84LEE660 pKa = 3.65PHH662 pKa = 6.2NLAKK666 pKa = 10.86GFGLGGAGEE675 pKa = 4.19PALDD679 pKa = 3.59QMDD682 pKa = 3.74RR683 pKa = 11.84VMADD687 pKa = 3.42FFPGFGFKK695 pKa = 9.86QDD697 pKa = 3.36AEE699 pKa = 4.56GFISLDD705 pKa = 3.51GEE707 pKa = 4.42GDD709 pKa = 3.4FAGIGFDD716 pKa = 5.77LMLTPDD722 pKa = 5.47AIRR725 pKa = 11.84EE726 pKa = 4.31APEE729 pKa = 3.95GTPEE733 pKa = 5.52GITEE737 pKa = 4.2DD738 pKa = 3.65PMTGEE743 pKa = 4.17FLVTTDD749 pKa = 3.05HH750 pKa = 6.69GKK752 pKa = 9.75QFGVSFGPPNMTCMAKK768 pKa = 10.56ALGVGDD774 pKa = 4.0TNNPGSQLSMDD785 pKa = 3.72QNGIAVFMPATTSGTSNRR803 pKa = 11.84RR804 pKa = 11.84QFSNNPQSFKK814 pKa = 10.84PSGGSSNFRR823 pKa = 11.84RR824 pKa = 11.84SQQAAGLYY832 pKa = 7.23TYY834 pKa = 8.27PARR837 pKa = 11.84RR838 pKa = 11.84TGEE841 pKa = 3.87NPFAEE846 pKa = 4.36LVYY849 pKa = 10.77GQDD852 pKa = 3.27TGVEE856 pKa = 4.23EE857 pKa = 4.65CAGLTQTVYY866 pKa = 8.85PTVPDD871 pKa = 3.71ADD873 pKa = 4.36LFMEE877 pKa = 4.63MLANLLGIPTTDD889 pKa = 2.09IYY891 pKa = 11.46QRR893 pKa = 11.84VDD895 pKa = 2.8GSFRR899 pKa = 11.84VQLGPDD905 pKa = 3.76LYY907 pKa = 11.04DD908 pKa = 3.3IVPDD912 pKa = 4.56FVTEE916 pKa = 3.99APNIARR922 pKa = 11.84GTEE925 pKa = 3.89QATQLATDD933 pKa = 3.63AAGNFMMDD941 pKa = 2.97AQDD944 pKa = 4.12RR945 pKa = 11.84FILHH949 pKa = 7.12APYY952 pKa = 10.14QSRR955 pKa = 11.84KK956 pKa = 9.82LVIPLILKK964 pKa = 9.21VVVPQQ969 pKa = 3.66

Molecular weight: 104.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6FCM3|A0A1H6FCM3_9GAMM Histidinol-phosphate aminotransferase OS=Thiotrichales bacterium HS_08 OX=1899563 GN=hisC2_1 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.54RR3 pKa = 11.84TFQPSQLVRR12 pKa = 11.84KK13 pKa = 9.03RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MQTRR25 pKa = 11.84GGRR28 pKa = 11.84QILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.45GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4918

0

4918

1586916

29

4142

322.7

36.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.354 ± 0.042

1.053 ± 0.013

5.419 ± 0.034

6.108 ± 0.04

4.04 ± 0.023

6.295 ± 0.043

2.514 ± 0.022

6.392 ± 0.032

5.463 ± 0.046

11.129 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.017

4.47 ± 0.031

4.203 ± 0.024

5.634 ± 0.044

4.759 ± 0.034

6.153 ± 0.032

5.276 ± 0.039

5.775 ± 0.034

1.382 ± 0.016

3.254 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski