Amino acid dipepetide frequency for Thiotrichales bacterium HS_08

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.532AlaAla: 7.532 ± 0.103	1.066AlaCys: 1.066 ± 0.029	4.762AlaAsp: 4.762 ± 0.072	5.557AlaGlu: 5.557 ± 0.075	3.144AlaPhe: 3.144 ± 0.049	5.813AlaGly: 5.813 ± 0.095	1.883AlaHis: 1.883 ± 0.035	5.057AlaIle: 5.057 ± 0.059	4.344AlaLys: 4.344 ± 0.07	10.004AlaLeu: 10.004 ± 0.103	2.054AlaMet: 2.054 ± 0.045	3.264AlaAsn: 3.264 ± 0.049	2.868AlaPro: 2.868 ± 0.052	4.799AlaGln: 4.799 ± 0.085	3.713AlaArg: 3.713 ± 0.054	4.599AlaSer: 4.599 ± 0.061	4.028AlaThr: 4.028 ± 0.06	5.174AlaVal: 5.174 ± 0.061	1.115AlaTrp: 1.115 ± 0.031	2.752AlaTyr: 2.752 ± 0.048	0.007AlaXaa: 0.007 ± 0.002
Cys	0.788CysAla: 0.788 ± 0.024	0.172CysCys: 0.172 ± 0.012	0.53CysAsp: 0.53 ± 0.018	0.543CysGlu: 0.543 ± 0.02	0.503CysPhe: 0.503 ± 0.017	0.774CysGly: 0.774 ± 0.025	0.403CysHis: 0.403 ± 0.026	0.641CysIle: 0.641 ± 0.02	0.473CysLys: 0.473 ± 0.018	1.2CysLeu: 1.2 ± 0.029	0.229CysMet: 0.229 ± 0.013	0.417CysAsn: 0.417 ± 0.02	0.514CysPro: 0.514 ± 0.019	0.602CysGln: 0.602 ± 0.022	0.529CysArg: 0.529 ± 0.019	0.651CysSer: 0.651 ± 0.025	0.483CysThr: 0.483 ± 0.018	0.542CysVal: 0.542 ± 0.019	0.177CysTrp: 0.177 ± 0.014	0.357CysTyr: 0.357 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.522AspAla: 4.522 ± 0.07	0.602AspCys: 0.602 ± 0.019	3.0AspAsp: 3.0 ± 0.063	3.415AspGlu: 3.415 ± 0.063	2.72AspPhe: 2.72 ± 0.053	3.698AspGly: 3.698 ± 0.092	0.917AspHis: 0.917 ± 0.026	4.045AspIle: 4.045 ± 0.063	3.461AspLys: 3.461 ± 0.049	5.442AspLeu: 5.442 ± 0.065	1.381AspMet: 1.381 ± 0.031	2.821AspAsn: 2.821 ± 0.051	2.018AspPro: 2.018 ± 0.042	1.571AspGln: 1.571 ± 0.032	1.994AspArg: 1.994 ± 0.037	3.283AspSer: 3.283 ± 0.051	2.979AspThr: 2.979 ± 0.048	3.1AspVal: 3.1 ± 0.048	0.996AspTrp: 0.996 ± 0.028	2.224AspTyr: 2.224 ± 0.04	0.003AspXaa: 0.003 ± 0.001
Glu	5.335GluAla: 5.335 ± 0.081	0.428GluCys: 0.428 ± 0.018	3.04GluAsp: 3.04 ± 0.057	3.608GluGlu: 3.608 ± 0.068	2.107GluPhe: 2.107 ± 0.041	3.042GluGly: 3.042 ± 0.056	1.563GluHis: 1.563 ± 0.031	4.4GluIle: 4.4 ± 0.061	4.071GluLys: 4.071 ± 0.07	6.139GluLeu: 6.139 ± 0.086	1.547GluMet: 1.547 ± 0.037	3.242GluAsn: 3.242 ± 0.049	1.939GluPro: 1.939 ± 0.04	4.489GluGln: 4.489 ± 0.065	3.105GluArg: 3.105 ± 0.05	3.178GluSer: 3.178 ± 0.052	3.713GluThr: 3.713 ± 0.059	3.565GluVal: 3.565 ± 0.058	0.703GluTrp: 0.703 ± 0.02	1.864GluTyr: 1.864 ± 0.04	0.001GluXaa: 0.001 ± 0.001
Phe	3.083PheAla: 3.083 ± 0.049	0.533PheCys: 0.533 ± 0.02	2.457PheAsp: 2.457 ± 0.045	2.358PheGlu: 2.358 ± 0.037	1.894PhePhe: 1.894 ± 0.042	2.457PheGly: 2.457 ± 0.047	0.958PheHis: 0.958 ± 0.026	2.925PheIle: 2.925 ± 0.058	2.035PheLys: 2.035 ± 0.04	3.842PheLeu: 3.842 ± 0.055	0.968PheMet: 0.968 ± 0.024	2.034PheAsn: 2.034 ± 0.042	1.589PhePro: 1.589 ± 0.033	1.726PheGln: 1.726 ± 0.035	1.661PheArg: 1.661 ± 0.032	3.383PheSer: 3.383 ± 0.055	2.226PheThr: 2.226 ± 0.036	2.163PheVal: 2.163 ± 0.041	0.573PheTrp: 0.573 ± 0.018	1.538PheTyr: 1.538 ± 0.035	0.003PheXaa: 0.003 ± 0.001
Gly	4.357GlyAla: 4.357 ± 0.074	0.756GlyCys: 0.756 ± 0.024	3.406GlyAsp: 3.406 ± 0.064	3.94GlyGlu: 3.94 ± 0.054	2.981GlyPhe: 2.981 ± 0.048	4.471GlyGly: 4.471 ± 0.089	1.546GlyHis: 1.546 ± 0.034	4.481GlyIle: 4.481 ± 0.05	4.104GlyLys: 4.104 ± 0.059	6.839GlyLeu: 6.839 ± 0.092	1.739GlyMet: 1.739 ± 0.042	2.874GlyAsn: 2.874 ± 0.069	1.246GlyPro: 1.246 ± 0.029	3.138GlyGln: 3.138 ± 0.059	2.918GlyArg: 2.918 ± 0.048	3.834GlySer: 3.834 ± 0.084	3.294GlyThr: 3.294 ± 0.074	3.913GlyVal: 3.913 ± 0.069	0.888GlyTrp: 0.888 ± 0.027	2.219GlyTyr: 2.219 ± 0.041	0.002GlyXaa: 0.002 ± 0.001
His	1.88HisAla: 1.88 ± 0.039	0.377HisCys: 0.377 ± 0.018	1.187HisAsp: 1.187 ± 0.028	1.376HisGlu: 1.376 ± 0.032	1.253HisPhe: 1.253 ± 0.028	1.47HisGly: 1.47 ± 0.037	0.783HisHis: 0.783 ± 0.025	1.683HisIle: 1.683 ± 0.035	1.216HisLys: 1.216 ± 0.029	2.921HisLeu: 2.921 ± 0.046	0.427HisMet: 0.427 ± 0.019	1.054HisAsn: 1.054 ± 0.026	1.38HisPro: 1.38 ± 0.033	1.379HisGln: 1.379 ± 0.034	1.161HisArg: 1.161 ± 0.029	1.517HisSer: 1.517 ± 0.032	1.25HisThr: 1.25 ± 0.032	1.205HisVal: 1.205 ± 0.027	0.499HisTrp: 0.499 ± 0.019	1.125HisTyr: 1.125 ± 0.027	0.002HisXaa: 0.002 ± 0.001
Ile	5.633IleAla: 5.633 ± 0.064	0.669IleCys: 0.669 ± 0.022	3.728IleAsp: 3.728 ± 0.05	4.225IleGlu: 4.225 ± 0.058	2.521IlePhe: 2.521 ± 0.047	3.761IleGly: 3.761 ± 0.056	1.637IleHis: 1.637 ± 0.033	4.022IleIle: 4.022 ± 0.065	3.673IleLys: 3.673 ± 0.054	6.334IleLeu: 6.334 ± 0.071	1.215IleMet: 1.215 ± 0.032	3.017IleAsn: 3.017 ± 0.047	2.931IlePro: 2.931 ± 0.044	3.156IleGln: 3.156 ± 0.047	3.01IleArg: 3.01 ± 0.047	4.56IleSer: 4.56 ± 0.056	3.746IleThr: 3.746 ± 0.054	3.315IleVal: 3.315 ± 0.051	0.792IleTrp: 0.792 ± 0.026	1.974IleTyr: 1.974 ± 0.041	0.004IleXaa: 0.004 ± 0.002
Lys	4.397LysAla: 4.397 ± 0.063	0.347LysCys: 0.347 ± 0.018	2.57LysAsp: 2.57 ± 0.048	2.911LysGlu: 2.911 ± 0.069	1.571LysPhe: 1.571 ± 0.037	2.732LysGly: 2.732 ± 0.048	1.422LysHis: 1.422 ± 0.032	3.923LysIle: 3.923 ± 0.07	3.826LysLys: 3.826 ± 0.081	5.629LysLeu: 5.629 ± 0.077	1.348LysMet: 1.348 ± 0.036	3.153LysAsn: 3.153 ± 0.054	2.691LysPro: 2.691 ± 0.052	3.598LysGln: 3.598 ± 0.063	2.686LysArg: 2.686 ± 0.049	2.997LysSer: 2.997 ± 0.056	3.604LysThr: 3.604 ± 0.059	2.996LysVal: 2.996 ± 0.046	0.549LysTrp: 0.549 ± 0.018	1.676LysTyr: 1.676 ± 0.037	0.002LysXaa: 0.002 ± 0.001
Leu	9.113LeuAla: 9.113 ± 0.112	1.156LeuCys: 1.156 ± 0.032	6.117LeuAsp: 6.117 ± 0.078	7.12LeuGlu: 7.12 ± 0.086	4.635LeuPhe: 4.635 ± 0.057	6.317LeuGly: 6.317 ± 0.07	2.801LeuHis: 2.801 ± 0.047	6.313LeuIle: 6.313 ± 0.071	6.239LeuLys: 6.239 ± 0.086	13.053LeuLeu: 13.053 ± 0.151	2.438LeuMet: 2.438 ± 0.042	5.049LeuAsn: 5.049 ± 0.061	5.507LeuPro: 5.507 ± 0.075	6.033LeuGln: 6.033 ± 0.094	5.532LeuArg: 5.532 ± 0.076	8.251LeuSer: 8.251 ± 0.083	5.229LeuThr: 5.229 ± 0.069	5.673LeuVal: 5.673 ± 0.078	1.471LeuTrp: 1.471 ± 0.046	3.24LeuTyr: 3.24 ± 0.051	0.005LeuXaa: 0.005 ± 0.002
Met	1.97MetAla: 1.97 ± 0.04	0.149MetCys: 0.149 ± 0.009	1.383MetAsp: 1.383 ± 0.028	1.301MetGlu: 1.301 ± 0.03	0.672MetPhe: 0.672 ± 0.02	1.47MetGly: 1.47 ± 0.037	0.502MetHis: 0.502 ± 0.018	1.279MetIle: 1.279 ± 0.027	1.262MetLys: 1.262 ± 0.031	2.624MetLeu: 2.624 ± 0.044	0.624MetMet: 0.624 ± 0.024	1.056MetAsn: 1.056 ± 0.03	1.375MetPro: 1.375 ± 0.029	1.378MetGln: 1.378 ± 0.028	1.227MetArg: 1.227 ± 0.03	1.542MetSer: 1.542 ± 0.032	1.196MetThr: 1.196 ± 0.029	1.27MetVal: 1.27 ± 0.035	0.167MetTrp: 0.167 ± 0.01	0.461MetTyr: 0.461 ± 0.018	0.001MetXaa: 0.001 ± 0.001
Asn	3.718AsnAla: 3.718 ± 0.057	0.452AsnCys: 0.452 ± 0.017	2.114AsnAsp: 2.114 ± 0.042	2.339AsnGlu: 2.339 ± 0.049	1.698AsnPhe: 1.698 ± 0.035	2.864AsnGly: 2.864 ± 0.066	1.141AsnHis: 1.141 ± 0.028	3.267AsnIle: 3.267 ± 0.055	2.503AsnLys: 2.503 ± 0.052	4.821AsnLeu: 4.821 ± 0.055	1.018AsnMet: 1.018 ± 0.024	2.312AsnAsn: 2.312 ± 0.049	2.463AsnPro: 2.463 ± 0.043	2.39AsnGln: 2.39 ± 0.042	2.04AsnArg: 2.04 ± 0.036	2.58AsnSer: 2.58 ± 0.053	2.702AsnThr: 2.702 ± 0.047	1.987AsnVal: 1.987 ± 0.039	0.737AsnTrp: 0.737 ± 0.023	1.551AsnTyr: 1.551 ± 0.039	0.003AsnXaa: 0.003 ± 0.001
Pro	3.656ProAla: 3.656 ± 0.059	0.346ProCys: 0.346 ± 0.016	2.994ProAsp: 2.994 ± 0.052	3.674ProGlu: 3.674 ± 0.051	1.6ProPhe: 1.6 ± 0.031	2.877ProGly: 2.877 ± 0.053	0.972ProHis: 0.972 ± 0.023	2.192ProIle: 2.192 ± 0.038	1.935ProLys: 1.935 ± 0.038	4.446ProLeu: 4.446 ± 0.064	0.875ProMet: 0.875 ± 0.024	1.541ProAsn: 1.541 ± 0.029	1.973ProPro: 1.973 ± 0.051	2.143ProGln: 2.143 ± 0.039	1.56ProArg: 1.56 ± 0.042	2.12ProSer: 2.12 ± 0.041	1.909ProThr: 1.909 ± 0.039	3.423ProVal: 3.423 ± 0.056	0.535ProTrp: 0.535 ± 0.019	1.254ProTyr: 1.254 ± 0.034	0.003ProXaa: 0.003 ± 0.001
Gln	6.141GlnAla: 6.141 ± 0.086	0.49GlnCys: 0.49 ± 0.019	2.567GlnAsp: 2.567 ± 0.048	3.035GlnGlu: 3.035 ± 0.055	1.778GlnPhe: 1.778 ± 0.036	3.533GlnGly: 3.533 ± 0.059	1.681GlnHis: 1.681 ± 0.039	3.121GlnIle: 3.121 ± 0.053	2.763GlnLys: 2.763 ± 0.043	5.961GlnLeu: 5.961 ± 0.098	1.161GlnMet: 1.161 ± 0.03	2.215GlnAsn: 2.215 ± 0.04	2.008GlnPro: 2.008 ± 0.041	4.923GlnGln: 4.923 ± 0.094	3.221GlnArg: 3.221 ± 0.055	2.922GlnSer: 2.922 ± 0.049	2.897GlnThr: 2.897 ± 0.049	3.521GlnVal: 3.521 ± 0.051	0.894GlnTrp: 0.894 ± 0.027	1.51GlnTyr: 1.51 ± 0.033	0.003GlnXaa: 0.003 ± 0.001
Arg	3.48ArgAla: 3.48 ± 0.05	0.513ArgCys: 0.513 ± 0.022	2.415ArgAsp: 2.415 ± 0.041	2.907ArgGlu: 2.907 ± 0.049	2.3ArgPhe: 2.3 ± 0.039	2.596ArgGly: 2.596 ± 0.049	1.386ArgHis: 1.386 ± 0.034	3.213ArgIle: 3.213 ± 0.05	2.696ArgLys: 2.696 ± 0.054	5.838ArgLeu: 5.838 ± 0.079	1.194ArgMet: 1.194 ± 0.028	1.962ArgAsn: 1.962 ± 0.037	1.718ArgPro: 1.718 ± 0.035	2.873ArgGln: 2.873 ± 0.046	2.534ArgArg: 2.534 ± 0.048	2.531ArgSer: 2.531 ± 0.046	2.079ArgThr: 2.079 ± 0.038	2.746ArgVal: 2.746 ± 0.044	0.76ArgTrp: 0.76 ± 0.025	1.852ArgTyr: 1.852 ± 0.037	0.003ArgXaa: 0.003 ± 0.001
Ser	5.068SerAla: 5.068 ± 0.07	0.686SerCys: 0.686 ± 0.02	3.199SerAsp: 3.199 ± 0.048	3.453SerGlu: 3.453 ± 0.051	2.5SerPhe: 2.5 ± 0.04	4.916SerGly: 4.916 ± 0.088	1.614SerHis: 1.614 ± 0.032	3.942SerIle: 3.942 ± 0.058	3.055SerLys: 3.055 ± 0.053	7.042SerLeu: 7.042 ± 0.071	1.362SerMet: 1.362 ± 0.028	2.696SerAsn: 2.696 ± 0.048	2.635SerPro: 2.635 ± 0.045	3.045SerGln: 3.045 ± 0.05	2.903SerArg: 2.903 ± 0.041	4.004SerSer: 4.004 ± 0.065	3.111SerThr: 3.111 ± 0.05	3.576SerVal: 3.576 ± 0.057	0.887SerTrp: 0.887 ± 0.022	1.831SerTyr: 1.831 ± 0.037	0.001SerXaa: 0.001 ± 0.001
Thr	4.647ThrAla: 4.647 ± 0.067	0.567ThrCys: 0.567 ± 0.024	2.996ThrAsp: 2.996 ± 0.06	3.25ThrGlu: 3.25 ± 0.049	1.702ThrPhe: 1.702 ± 0.038	4.29ThrGly: 4.29 ± 0.074	1.316ThrHis: 1.316 ± 0.027	3.023ThrIle: 3.023 ± 0.051	1.993ThrLys: 1.993 ± 0.042	6.63ThrLeu: 6.63 ± 0.08	0.982ThrMet: 0.982 ± 0.025	1.757ThrAsn: 1.757 ± 0.04	3.046ThrPro: 3.046 ± 0.052	2.964ThrGln: 2.964 ± 0.05	2.315ThrArg: 2.315 ± 0.039	2.923ThrSer: 2.923 ± 0.048	2.869ThrThr: 2.869 ± 0.063	3.269ThrVal: 3.269 ± 0.06	0.692ThrTrp: 0.692 ± 0.021	1.53ThrTyr: 1.53 ± 0.033	0.001ThrXaa: 0.001 ± 0.001
Val	4.76ValAla: 4.76 ± 0.056	0.687ValCys: 0.687 ± 0.022	3.357ValAsp: 3.357 ± 0.05	3.586ValGlu: 3.586 ± 0.059	2.559ValPhe: 2.559 ± 0.047	3.24ValGly: 3.24 ± 0.045	1.263ValHis: 1.263 ± 0.028	3.799ValIle: 3.799 ± 0.057	2.911ValLys: 2.911 ± 0.053	6.653ValLeu: 6.653 ± 0.071	1.393ValMet: 1.393 ± 0.033	2.425ValAsn: 2.425 ± 0.041	2.18ValPro: 2.18 ± 0.037	2.603ValGln: 2.603 ± 0.044	2.792ValArg: 2.792 ± 0.047	3.902ValSer: 3.902 ± 0.064	3.218ValThr: 3.218 ± 0.06	3.822ValVal: 3.822 ± 0.063	0.694ValTrp: 0.694 ± 0.027	1.907ValTyr: 1.907 ± 0.036	0.003ValXaa: 0.003 ± 0.001
Trp	0.854TrpAla: 0.854 ± 0.022	0.158TrpCys: 0.158 ± 0.009	0.679TrpAsp: 0.679 ± 0.026	0.723TrpGlu: 0.723 ± 0.024	0.639TrpPhe: 0.639 ± 0.022	0.726TrpGly: 0.726 ± 0.024	0.376TrpHis: 0.376 ± 0.017	0.797TrpIle: 0.797 ± 0.025	0.619TrpLys: 0.619 ± 0.019	2.122TrpLeu: 2.122 ± 0.047	0.359TrpMet: 0.359 ± 0.015	0.527TrpAsn: 0.527 ± 0.02	0.396TrpPro: 0.396 ± 0.017	1.177TrpGln: 1.177 ± 0.027	0.807TrpArg: 0.807 ± 0.03	0.766TrpSer: 0.766 ± 0.025	0.532TrpThr: 0.532 ± 0.02	0.918TrpVal: 0.918 ± 0.028	0.207TrpTrp: 0.207 ± 0.013	0.441TrpTyr: 0.441 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.601TyrAla: 2.601 ± 0.045	0.371TyrCys: 0.371 ± 0.016	1.693TyrAsp: 1.693 ± 0.041	1.759TyrGlu: 1.759 ± 0.034	1.624TyrPhe: 1.624 ± 0.035	1.896TyrGly: 1.896 ± 0.034	0.977TyrHis: 0.977 ± 0.029	1.798TyrIle: 1.798 ± 0.04	1.447TyrLys: 1.447 ± 0.038	3.748TyrLeu: 3.748 ± 0.049	0.592TyrMet: 0.592 ± 0.021	1.281TyrAsn: 1.281 ± 0.033	1.55TyrPro: 1.55 ± 0.037	2.356TyrGln: 2.356 ± 0.041	1.881TyrArg: 1.881 ± 0.038	1.977TyrSer: 1.977 ± 0.037	1.694TyrThr: 1.694 ± 0.039	1.572TyrVal: 1.572 ± 0.034	0.486TyrTrp: 0.486 ± 0.017	1.238TyrTyr: 1.238 ± 0.033	0.003TyrXaa: 0.003 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.004XaaPhe: 0.004 ± 0.002	0.003XaaGly: 0.003 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.003XaaIle: 0.003 ± 0.001	0.003XaaLys: 0.003 ± 0.001	0.006XaaLeu: 0.006 ± 0.002	0.005XaaMet: 0.005 ± 0.002	0.002XaaAsn: 0.002 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.002	0.004XaaSer: 0.004 ± 0.001	0.003XaaThr: 0.003 ± 0.001	0.003XaaVal: 0.003 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.002XaaTyr: 0.002 ± 0.001	0.301XaaXaa: 0.301 ± 0.039

Statistics based on 4918 proteins (1586916 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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