Escherichia phage LAMP
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I6PD12|A0A2I6PD12_9CAUD Uncharacterized protein OS=Escherichia phage LAMP OX=2065191 GN=phiL_039 PE=4 SV=1
MM1 pKa = 7.42 INKK4 pKa = 9.58 IKK6 pKa = 10.86 DD7 pKa = 3.45 NEE9 pKa = 4.13 YY10 pKa = 10.85 LLNSSILDD18 pKa = 3.53 NGNTIDD24 pKa = 6.29 FITLSEE30 pKa = 4.15 LKK32 pKa = 10.55 EE33 pKa = 4.19 LVNSLYY39 pKa = 10.81 SEE41 pKa = 4.11 EE42 pKa = 4.1 QNRR45 pKa = 11.84 LLQEE49 pKa = 4.31 SLSGDD54 pKa = 4.09 YY55 pKa = 11.19 VCDD58 pKa = 3.48 GCLII62 pKa = 4.04
Molecular weight: 7.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.822
IPC2_protein 4.075
IPC_protein 3.923
Toseland 3.745
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.757
Grimsley 3.656
Solomon 3.872
Lehninger 3.821
Nozaki 4.024
DTASelect 4.164
Thurlkill 3.795
EMBOSS 3.821
Sillero 4.037
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 4.012
IPC2.peptide.svr19 3.939
Protein with the highest isoelectric point:
>tr|A0A2I6PD32|A0A2I6PD32_9CAUD Uncharacterized protein OS=Escherichia phage LAMP OX=2065191 GN=phiL_070 PE=4 SV=1
MM1 pKa = 7.44 SRR3 pKa = 11.84 LHH5 pKa = 5.61 STKK8 pKa = 10.26 KK9 pKa = 10.3 NNFYY13 pKa = 9.99 RR14 pKa = 11.84 QRR16 pKa = 11.84 RR17 pKa = 11.84 ARR19 pKa = 11.84 SNASRR24 pKa = 11.84 KK25 pKa = 8.17 MKK27 pKa = 10.13 GMQLTYY33 pKa = 10.77 EE34 pKa = 4.14 QIKK37 pKa = 9.94 QINNIISQVVWEE49 pKa = 4.43 VTVGEE54 pKa = 4.44 PEE56 pKa = 3.65
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.304
IPC2_protein 9.882
IPC_protein 10.657
Toseland 10.891
ProMoST 10.555
Dawson 10.965
Bjellqvist 10.657
Wikipedia 11.169
Rodwell 11.272
Grimsley 11.008
Solomon 11.096
Lehninger 11.067
Nozaki 10.862
DTASelect 10.657
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.891
Patrickios 11.052
IPC_peptide 11.096
IPC2_peptide 9.399
IPC2.peptide.svr19 8.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
20410
38
1450
214.8
24.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.143 ± 0.534
1.068 ± 0.148
6.164 ± 0.28
7.075 ± 0.381
3.797 ± 0.158
7.134 ± 0.357
1.671 ± 0.145
5.806 ± 0.206
6.002 ± 0.338
7.202 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.685 ± 0.189
5.884 ± 0.253
3.704 ± 0.165
4.429 ± 0.318
5.365 ± 0.274
6.786 ± 0.231
5.698 ± 0.311
6.585 ± 0.28
1.372 ± 0.117
3.43 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here