Amino acid dipepetide frequency for Escherichia phage LAMP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.84AlaAla: 7.84 ± 1.102
0.637AlaCys: 0.637 ± 0.168
5.635AlaAsp: 5.635 ± 0.497
5.488AlaGlu: 5.488 ± 0.971
3.087AlaPhe: 3.087 ± 0.341
5.537AlaGly: 5.537 ± 0.536
1.176AlaHis: 1.176 ± 0.259
4.116AlaIle: 4.116 ± 0.426
5.047AlaLys: 5.047 ± 0.818
7.252AlaLeu: 7.252 ± 0.633
2.205AlaMet: 2.205 ± 0.297
4.214AlaAsn: 4.214 ± 0.493
3.087AlaPro: 3.087 ± 0.338
4.508AlaGln: 4.508 ± 0.792
3.822AlaArg: 3.822 ± 0.668
5.194AlaSer: 5.194 ± 0.557
4.018AlaThr: 4.018 ± 0.494
5.096AlaVal: 5.096 ± 0.507
1.029AlaTrp: 1.029 ± 0.202
2.45AlaTyr: 2.45 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
0.98CysAla: 0.98 ± 0.254
0.0CysCys: 0.0 ± 0.0
0.49CysAsp: 0.49 ± 0.189
0.637CysGlu: 0.637 ± 0.179
0.294CysPhe: 0.294 ± 0.115
0.931CysGly: 0.931 ± 0.266
0.245CysHis: 0.245 ± 0.109
0.637CysIle: 0.637 ± 0.208
0.98CysLys: 0.98 ± 0.257
0.882CysLeu: 0.882 ± 0.242
0.392CysMet: 0.392 ± 0.143
0.343CysAsn: 0.343 ± 0.131
0.343CysPro: 0.343 ± 0.15
0.343CysGln: 0.343 ± 0.138
0.49CysArg: 0.49 ± 0.15
0.833CysSer: 0.833 ± 0.248
0.539CysThr: 0.539 ± 0.173
0.833CysVal: 0.833 ± 0.258
0.049CysTrp: 0.049 ± 0.056
0.441CysTyr: 0.441 ± 0.151
0.0CysXaa: 0.0 ± 0.0
Asp
5.439AspAla: 5.439 ± 0.492
0.539AspCys: 0.539 ± 0.162
3.234AspAsp: 3.234 ± 0.471
4.9AspGlu: 4.9 ± 0.661
2.009AspPhe: 2.009 ± 0.31
4.9AspGly: 4.9 ± 0.579
0.735AspHis: 0.735 ± 0.211
4.655AspIle: 4.655 ± 0.483
3.332AspLys: 3.332 ± 0.431
4.851AspLeu: 4.851 ± 0.434
1.96AspMet: 1.96 ± 0.356
3.969AspAsn: 3.969 ± 0.425
2.499AspPro: 2.499 ± 0.47
1.813AspGln: 1.813 ± 0.308
2.646AspArg: 2.646 ± 0.4
3.773AspSer: 3.773 ± 0.41
2.793AspThr: 2.793 ± 0.357
4.361AspVal: 4.361 ± 0.342
0.735AspTrp: 0.735 ± 0.186
2.45AspTyr: 2.45 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
6.713GluAla: 6.713 ± 0.831
1.029GluCys: 1.029 ± 0.282
4.312GluAsp: 4.312 ± 0.685
7.546GluGlu: 7.546 ± 0.933
2.891GluPhe: 2.891 ± 0.375
4.165GluGly: 4.165 ± 0.398
1.274GluHis: 1.274 ± 0.335
3.822GluIle: 3.822 ± 0.402
4.361GluLys: 4.361 ± 0.5
5.341GluLeu: 5.341 ± 0.6
2.499GluMet: 2.499 ± 0.348
3.185GluAsn: 3.185 ± 0.415
1.813GluPro: 1.813 ± 0.381
2.891GluGln: 2.891 ± 0.484
4.263GluArg: 4.263 ± 0.612
3.675GluSer: 3.675 ± 0.424
3.038GluThr: 3.038 ± 0.381
4.557GluVal: 4.557 ± 0.603
1.078GluTrp: 1.078 ± 0.237
2.303GluTyr: 2.303 ± 0.301
0.0GluXaa: 0.0 ± 0.0
Phe
2.891PheAla: 2.891 ± 0.416
0.539PheCys: 0.539 ± 0.195
3.185PheAsp: 3.185 ± 0.498
2.156PheGlu: 2.156 ± 0.415
1.225PhePhe: 1.225 ± 0.294
2.352PheGly: 2.352 ± 0.315
0.588PheHis: 0.588 ± 0.215
1.911PheIle: 1.911 ± 0.249
1.911PheLys: 1.911 ± 0.346
3.087PheLeu: 3.087 ± 0.484
0.784PheMet: 0.784 ± 0.19
2.793PheAsn: 2.793 ± 0.386
0.98PhePro: 0.98 ± 0.244
1.715PheGln: 1.715 ± 0.273
2.352PheArg: 2.352 ± 0.297
2.009PheSer: 2.009 ± 0.306
2.254PheThr: 2.254 ± 0.373
2.744PheVal: 2.744 ± 0.312
0.343PheTrp: 0.343 ± 0.133
2.156PheTyr: 2.156 ± 0.389
0.0PheXaa: 0.0 ± 0.0
Gly
5.586GlyAla: 5.586 ± 0.717
0.882GlyCys: 0.882 ± 0.227
4.067GlyAsp: 4.067 ± 0.539
4.361GlyGlu: 4.361 ± 0.454
2.94GlyPhe: 2.94 ± 0.337
5.831GlyGly: 5.831 ± 0.818
0.931GlyHis: 0.931 ± 0.214
4.214GlyIle: 4.214 ± 0.437
4.41GlyLys: 4.41 ± 0.469
4.802GlyLeu: 4.802 ± 0.595
2.156GlyMet: 2.156 ± 0.32
3.577GlyAsn: 3.577 ± 0.542
0.833GlyPro: 0.833 ± 0.214
3.038GlyGln: 3.038 ± 0.433
4.116GlyArg: 4.116 ± 0.387
6.419GlySer: 6.419 ± 0.676
3.675GlyThr: 3.675 ± 0.527
5.978GlyVal: 5.978 ± 0.658
1.029GlyTrp: 1.029 ± 0.192
2.499GlyTyr: 2.499 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
0.882HisAla: 0.882 ± 0.242
0.049HisCys: 0.049 ± 0.049
0.98HisAsp: 0.98 ± 0.241
1.078HisGlu: 1.078 ± 0.247
0.588HisPhe: 0.588 ± 0.152
1.078HisGly: 1.078 ± 0.253
0.245HisHis: 0.245 ± 0.111
0.98HisIle: 0.98 ± 0.236
1.029HisLys: 1.029 ± 0.209
1.323HisLeu: 1.323 ± 0.231
0.294HisMet: 0.294 ± 0.124
1.568HisAsn: 1.568 ± 0.27
0.588HisPro: 0.588 ± 0.172
0.588HisGln: 0.588 ± 0.152
1.176HisArg: 1.176 ± 0.278
1.372HisSer: 1.372 ± 0.362
1.029HisThr: 1.029 ± 0.23
0.882HisVal: 0.882 ± 0.207
0.245HisTrp: 0.245 ± 0.103
0.735HisTyr: 0.735 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
3.577IleAla: 3.577 ± 0.328
0.784IleCys: 0.784 ± 0.24
4.165IleAsp: 4.165 ± 0.434
4.41IleGlu: 4.41 ± 0.57
2.107IlePhe: 2.107 ± 0.323
3.92IleGly: 3.92 ± 0.476
0.833IleHis: 0.833 ± 0.177
2.254IleIle: 2.254 ± 0.369
4.557IleLys: 4.557 ± 0.373
3.283IleLeu: 3.283 ± 0.379
1.176IleMet: 1.176 ± 0.294
3.43IleAsn: 3.43 ± 0.4
3.822IlePro: 3.822 ± 0.523
1.862IleGln: 1.862 ± 0.294
3.626IleArg: 3.626 ± 0.347
4.312IleSer: 4.312 ± 0.37
4.41IleThr: 4.41 ± 0.589
2.891IleVal: 2.891 ± 0.457
0.882IleTrp: 0.882 ± 0.203
1.764IleTyr: 1.764 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
5.537LysAla: 5.537 ± 0.973
0.735LysCys: 0.735 ± 0.226
4.018LysAsp: 4.018 ± 0.416
5.194LysGlu: 5.194 ± 0.612
2.058LysPhe: 2.058 ± 0.343
3.675LysGly: 3.675 ± 0.414
1.568LysHis: 1.568 ± 0.273
3.087LysIle: 3.087 ± 0.361
4.214LysLys: 4.214 ± 0.509
4.018LysLeu: 4.018 ± 0.365
1.911LysMet: 1.911 ± 0.3
3.038LysAsn: 3.038 ± 0.415
1.862LysPro: 1.862 ± 0.33
2.548LysGln: 2.548 ± 0.296
2.793LysArg: 2.793 ± 0.459
3.381LysSer: 3.381 ± 0.397
3.381LysThr: 3.381 ± 0.408
3.92LysVal: 3.92 ± 0.418
1.029LysTrp: 1.029 ± 0.256
2.058LysTyr: 2.058 ± 0.311
0.0LysXaa: 0.0 ± 0.0
Leu
5.635LeuAla: 5.635 ± 0.562
0.686LeuCys: 0.686 ± 0.237
4.753LeuAsp: 4.753 ± 0.517
5.586LeuGlu: 5.586 ± 0.531
2.548LeuPhe: 2.548 ± 0.387
4.998LeuGly: 4.998 ± 0.49
1.029LeuHis: 1.029 ± 0.231
3.773LeuIle: 3.773 ± 0.478
3.92LeuLys: 3.92 ± 0.493
4.802LeuLeu: 4.802 ± 0.544
2.548LeuMet: 2.548 ± 0.322
4.312LeuAsn: 4.312 ± 0.577
3.43LeuPro: 3.43 ± 0.344
3.136LeuGln: 3.136 ± 0.396
4.312LeuArg: 4.312 ± 0.492
4.998LeuSer: 4.998 ± 0.588
4.165LeuThr: 4.165 ± 0.503
4.704LeuVal: 4.704 ± 0.479
0.441LeuTrp: 0.441 ± 0.139
2.254LeuTyr: 2.254 ± 0.31
0.0LeuXaa: 0.0 ± 0.0
Met
2.303MetAla: 2.303 ± 0.311
0.343MetCys: 0.343 ± 0.154
1.568MetAsp: 1.568 ± 0.236
1.764MetGlu: 1.764 ± 0.312
1.274MetPhe: 1.274 ± 0.24
1.127MetGly: 1.127 ± 0.265
0.49MetHis: 0.49 ± 0.159
1.764MetIle: 1.764 ± 0.343
1.715MetLys: 1.715 ± 0.312
2.107MetLeu: 2.107 ± 0.393
0.735MetMet: 0.735 ± 0.208
1.617MetAsn: 1.617 ± 0.296
0.882MetPro: 0.882 ± 0.216
1.225MetGln: 1.225 ± 0.263
1.617MetArg: 1.617 ± 0.315
2.254MetSer: 2.254 ± 0.329
1.96MetThr: 1.96 ± 0.279
1.323MetVal: 1.323 ± 0.317
0.196MetTrp: 0.196 ± 0.122
0.588MetTyr: 0.588 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
5.145AsnAla: 5.145 ± 0.527
0.196AsnCys: 0.196 ± 0.095
2.401AsnAsp: 2.401 ± 0.258
2.45AsnGlu: 2.45 ± 0.379
2.597AsnPhe: 2.597 ± 0.359
5.39AsnGly: 5.39 ± 0.612
0.833AsnHis: 0.833 ± 0.227
3.871AsnIle: 3.871 ± 0.427
3.136AsnLys: 3.136 ± 0.355
4.606AsnLeu: 4.606 ± 0.568
1.225AsnMet: 1.225 ± 0.241
3.969AsnAsn: 3.969 ± 0.502
2.989AsnPro: 2.989 ± 0.401
3.087AsnGln: 3.087 ± 0.411
3.528AsnArg: 3.528 ± 0.401
3.773AsnSer: 3.773 ± 0.413
3.528AsnThr: 3.528 ± 0.4
3.528AsnVal: 3.528 ± 0.474
0.833AsnTrp: 0.833 ± 0.211
1.764AsnTyr: 1.764 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
2.94ProAla: 2.94 ± 0.36
0.392ProCys: 0.392 ± 0.137
2.695ProAsp: 2.695 ± 0.348
2.695ProGlu: 2.695 ± 0.393
1.715ProPhe: 1.715 ± 0.341
1.764ProGly: 1.764 ± 0.31
0.784ProHis: 0.784 ± 0.186
2.352ProIle: 2.352 ± 0.279
2.254ProLys: 2.254 ± 0.391
2.45ProLeu: 2.45 ± 0.348
0.931ProMet: 0.931 ± 0.227
1.617ProAsn: 1.617 ± 0.313
1.568ProPro: 1.568 ± 0.315
1.666ProGln: 1.666 ± 0.301
1.078ProArg: 1.078 ± 0.207
3.038ProSer: 3.038 ± 0.498
2.548ProThr: 2.548 ± 0.379
2.744ProVal: 2.744 ± 0.34
0.637ProTrp: 0.637 ± 0.148
1.176ProTyr: 1.176 ± 0.214
0.0ProXaa: 0.0 ± 0.0
Gln
4.459GlnAla: 4.459 ± 0.595
0.784GlnCys: 0.784 ± 0.201
2.205GlnAsp: 2.205 ± 0.358
2.793GlnGlu: 2.793 ± 0.42
1.666GlnPhe: 1.666 ± 0.225
2.891GlnGly: 2.891 ± 0.527
0.392GlnHis: 0.392 ± 0.158
2.548GlnIle: 2.548 ± 0.369
2.891GlnLys: 2.891 ± 0.352
2.891GlnLeu: 2.891 ± 0.405
1.127GlnMet: 1.127 ± 0.25
3.038GlnAsn: 3.038 ± 0.466
1.127GlnPro: 1.127 ± 0.19
3.626GlnGln: 3.626 ± 0.941
2.499GlnArg: 2.499 ± 0.431
2.058GlnSer: 2.058 ± 0.332
2.45GlnThr: 2.45 ± 0.281
2.45GlnVal: 2.45 ± 0.375
0.735GlnTrp: 0.735 ± 0.168
1.666GlnTyr: 1.666 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
4.116ArgAla: 4.116 ± 0.671
0.637ArgCys: 0.637 ± 0.166
3.283ArgAsp: 3.283 ± 0.406
3.822ArgGlu: 3.822 ± 0.527
2.352ArgPhe: 2.352 ± 0.389
3.381ArgGly: 3.381 ± 0.344
0.931ArgHis: 0.931 ± 0.235
3.185ArgIle: 3.185 ± 0.409
3.185ArgLys: 3.185 ± 0.454
3.822ArgLeu: 3.822 ± 0.434
1.323ArgMet: 1.323 ± 0.238
2.548ArgAsn: 2.548 ± 0.32
1.96ArgPro: 1.96 ± 0.286
2.352ArgGln: 2.352 ± 0.373
3.43ArgArg: 3.43 ± 0.507
3.92ArgSer: 3.92 ± 0.421
2.842ArgThr: 2.842 ± 0.377
4.116ArgVal: 4.116 ± 0.478
0.784ArgTrp: 0.784 ± 0.192
1.666ArgTyr: 1.666 ± 0.299
0.0ArgXaa: 0.0 ± 0.0
Ser
3.969SerAla: 3.969 ± 0.443
0.588SerCys: 0.588 ± 0.192
4.018SerAsp: 4.018 ± 0.574
3.969SerGlu: 3.969 ± 0.359
2.352SerPhe: 2.352 ± 0.368
6.468SerGly: 6.468 ± 0.761
1.421SerHis: 1.421 ± 0.287
3.92SerIle: 3.92 ± 0.477
3.871SerLys: 3.871 ± 0.443
5.39SerLeu: 5.39 ± 0.505
1.715SerMet: 1.715 ± 0.292
4.949SerAsn: 4.949 ± 0.631
2.793SerPro: 2.793 ± 0.348
2.744SerGln: 2.744 ± 0.455
3.675SerArg: 3.675 ± 0.367
4.802SerSer: 4.802 ± 0.45
3.822SerThr: 3.822 ± 0.45
3.969SerVal: 3.969 ± 0.533
1.029SerTrp: 1.029 ± 0.23
2.401SerTyr: 2.401 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
4.9ThrAla: 4.9 ± 0.696
0.294ThrCys: 0.294 ± 0.117
2.94ThrAsp: 2.94 ± 0.383
4.018ThrGlu: 4.018 ± 0.381
2.254ThrPhe: 2.254 ± 0.317
5.047ThrGly: 5.047 ± 0.543
0.882ThrHis: 0.882 ± 0.188
3.43ThrIle: 3.43 ± 0.498
2.94ThrLys: 2.94 ± 0.36
4.214ThrLeu: 4.214 ± 0.422
0.882ThrMet: 0.882 ± 0.2
3.332ThrAsn: 3.332 ± 0.464
2.891ThrPro: 2.891 ± 0.301
2.352ThrGln: 2.352 ± 0.379
2.303ThrArg: 2.303 ± 0.349
3.528ThrSer: 3.528 ± 0.473
3.136ThrThr: 3.136 ± 0.468
4.508ThrVal: 4.508 ± 0.482
0.931ThrTrp: 0.931 ± 0.243
2.205ThrTyr: 2.205 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
5.243ValAla: 5.243 ± 0.377
0.686ValCys: 0.686 ± 0.186
4.459ValAsp: 4.459 ± 0.529
4.508ValGlu: 4.508 ± 0.404
2.499ValPhe: 2.499 ± 0.367
4.655ValGly: 4.655 ± 0.379
1.372ValHis: 1.372 ± 0.209
4.165ValIle: 4.165 ± 0.489
3.626ValLys: 3.626 ± 0.432
4.165ValLeu: 4.165 ± 0.551
1.274ValMet: 1.274 ± 0.223
4.312ValAsn: 4.312 ± 0.493
1.862ValPro: 1.862 ± 0.313
2.597ValGln: 2.597 ± 0.462
2.989ValArg: 2.989 ± 0.282
4.851ValSer: 4.851 ± 0.772
4.655ValThr: 4.655 ± 0.672
4.704ValVal: 4.704 ± 0.531
1.127ValTrp: 1.127 ± 0.243
2.107ValTyr: 2.107 ± 0.322
0.0ValXaa: 0.0 ± 0.0
Trp
0.882TrpAla: 0.882 ± 0.217
0.392TrpCys: 0.392 ± 0.191
1.078TrpAsp: 1.078 ± 0.186
0.735TrpGlu: 0.735 ± 0.163
0.343TrpPhe: 0.343 ± 0.131
0.833TrpGly: 0.833 ± 0.228
0.294TrpHis: 0.294 ± 0.138
1.078TrpIle: 1.078 ± 0.254
0.735TrpLys: 0.735 ± 0.175
0.735TrpLeu: 0.735 ± 0.19
0.637TrpMet: 0.637 ± 0.191
0.98TrpAsn: 0.98 ± 0.252
0.294TrpPro: 0.294 ± 0.113
0.441TrpGln: 0.441 ± 0.148
0.686TrpArg: 0.686 ± 0.152
1.078TrpSer: 1.078 ± 0.255
1.127TrpThr: 1.127 ± 0.185
0.735TrpVal: 0.735 ± 0.176
0.147TrpTrp: 0.147 ± 0.103
0.49TrpTyr: 0.49 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.401TyrAla: 2.401 ± 0.264
0.49TyrCys: 0.49 ± 0.176
2.156TyrAsp: 2.156 ± 0.357
2.646TyrGlu: 2.646 ± 0.348
1.176TyrPhe: 1.176 ± 0.272
2.401TyrGly: 2.401 ± 0.379
0.686TyrHis: 0.686 ± 0.176
2.303TyrIle: 2.303 ± 0.296
1.911TyrLys: 1.911 ± 0.276
2.009TyrLeu: 2.009 ± 0.308
1.029TyrMet: 1.029 ± 0.237
2.058TyrAsn: 2.058 ± 0.251
1.421TyrPro: 1.421 ± 0.295
1.764TyrGln: 1.764 ± 0.229
2.254TyrArg: 2.254 ± 0.444
2.597TyrSer: 2.597 ± 0.308
1.617TyrThr: 1.617 ± 0.257
1.813TyrVal: 1.813 ± 0.313
0.441TyrTrp: 0.441 ± 0.127
1.127TyrTyr: 1.127 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (20410 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski