Marinicella litoralis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2897 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R6XMW7|A0A4R6XMW7_9GAMM Putative ABC transport system permease protein OS=Marinicella litoralis OX=644220 GN=C8D91_2016 PE=4 SV=1
MM1 pKa = 7.55 FFLCFILLSLISLNVTAQNYY21 pKa = 9.3 FSDD24 pKa = 3.81 VSNQAGVAINHH35 pKa = 5.32 QLKK38 pKa = 10.63 SGEE41 pKa = 4.18 LGMGQGWVDD50 pKa = 3.64 VNNDD54 pKa = 3.15 GYY56 pKa = 11.37 EE57 pKa = 4.12 DD58 pKa = 4.92 LMLTNQSGEE67 pKa = 3.83 NYY69 pKa = 10.51 LFINQQDD76 pKa = 3.55 GSFVEE81 pKa = 4.23 MAQYY85 pKa = 11.47 NDD87 pKa = 2.83 IKK89 pKa = 11.13 LPLSQDD95 pKa = 3.26 FGVSVTDD102 pKa = 3.78 FNNDD106 pKa = 2.48 GFQDD110 pKa = 3.81 LFIASWGVNKK120 pKa = 10.21 LLKK123 pKa = 10.39 NINGSSFLDD132 pKa = 3.66 VSDD135 pKa = 3.94 EE136 pKa = 4.03 MGFVDD141 pKa = 4.36 NSYY144 pKa = 11.07 SIASTWADD152 pKa = 2.94 INQDD156 pKa = 3.04 GWLDD160 pKa = 3.92 LYY162 pKa = 10.74 VVNYY166 pKa = 10.36 SVEE169 pKa = 4.08 NDD171 pKa = 2.98 SDD173 pKa = 3.76 EE174 pKa = 4.71 ADD176 pKa = 3.41 RR177 pKa = 11.84 MYY179 pKa = 11.32 LNNQGANFIDD189 pKa = 4.05 VSSDD193 pKa = 3.03 LTPFTNLTKK202 pKa = 10.6 HH203 pKa = 5.5 GLAVQFIDD211 pKa = 4.73 FDD213 pKa = 3.86 MDD215 pKa = 3.47 NDD217 pKa = 3.23 MDD219 pKa = 4.9 LYY221 pKa = 11.14 VVNDD225 pKa = 3.7 KK226 pKa = 11.4 LEE228 pKa = 4.02 QNTLWRR234 pKa = 11.84 NDD236 pKa = 3.45 GPGSSACGVYY246 pKa = 10.34 VCFTDD251 pKa = 5.29 VSALTQTNRR260 pKa = 11.84 AVFGMGIAIADD271 pKa = 3.74 YY272 pKa = 11.23 DD273 pKa = 4.43 LDD275 pKa = 4.97 GDD277 pKa = 3.83 FDD279 pKa = 5.77 FYY281 pKa = 11.08 FSSIAEE287 pKa = 4.05 QVLLQSQISQGSAVFVEE304 pKa = 4.45 QSNPAGLNFNATGWATIFADD324 pKa = 4.43 FNNDD328 pKa = 3.45 QFPDD332 pKa = 3.7 AYY334 pKa = 10.94 LATANNTPSKK344 pKa = 10.49 RR345 pKa = 11.84 DD346 pKa = 3.27 RR347 pKa = 11.84 LYY349 pKa = 11.45 LNNQSQQFTDD359 pKa = 3.52 VTALSGIEE367 pKa = 4.09 DD368 pKa = 4.52 LYY370 pKa = 9.13 MTVGAAKK377 pKa = 10.54 GDD379 pKa = 3.63 FDD381 pKa = 5.55 NDD383 pKa = 3.57 GKK385 pKa = 11.42 VDD387 pKa = 4.03 LVVGNWGEE395 pKa = 3.96 NYY397 pKa = 9.32 MLYY400 pKa = 10.87 KK401 pKa = 9.84 NTSLDD406 pKa = 3.36 ANNWIKK412 pKa = 10.61 IKK414 pKa = 10.82 LIGGSGINQLAIGSKK429 pKa = 9.81 VAVNTSNGQEE439 pKa = 4.17 LVDD442 pKa = 3.65 QLVSGGSHH450 pKa = 6.28 GAGNSLVLHH459 pKa = 6.63 FGLADD464 pKa = 3.53 EE465 pKa = 5.29 TIEE468 pKa = 4.29 SVKK471 pKa = 9.45 ITWPNGIISLLSGLSANQMHH491 pKa = 6.97 TLTYY495 pKa = 9.57 PGDD498 pKa = 4.24 GIFMQGFEE506 pKa = 4.14
Molecular weight: 55.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.671
IPC2_protein 3.719
IPC_protein 3.77
Toseland 3.528
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.884
Patrickios 1.354
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.811
Protein with the highest isoelectric point:
>tr|A0A4R6XV76|A0A4R6XV76_9GAMM RND family efflux transporter MFP subunit OS=Marinicella litoralis OX=644220 GN=C8D91_0928 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.08 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 6.05 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.55 TAGGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.74 GRR39 pKa = 11.84 KK40 pKa = 8.8 RR41 pKa = 11.84 LAAA44 pKa = 4.42
Molecular weight: 4.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2897
0
2897
1015597
26
4547
350.6
39.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.047 ± 0.046
0.94 ± 0.015
5.896 ± 0.041
5.589 ± 0.05
4.373 ± 0.037
6.738 ± 0.047
2.397 ± 0.026
6.722 ± 0.031
5.635 ± 0.056
9.972 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.027
5.172 ± 0.044
3.847 ± 0.03
5.02 ± 0.038
3.689 ± 0.033
6.579 ± 0.044
5.433 ± 0.045
6.804 ± 0.043
1.325 ± 0.018
3.117 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here