Enterobacteria phage HK620 (Bacteriophage HK620)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; Escherichia virus HK620

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZ14|Q9AZ14_BPHK6 Protein ninE OS=Enterobacteria phage HK620 OX=155148 GN=ninE PE=3 SV=1
MM1 pKa = 7.82IDD3 pKa = 3.4PNRR6 pKa = 11.84SYY8 pKa = 10.61EE9 pKa = 3.97QEE11 pKa = 4.12SVEE14 pKa = 5.1RR15 pKa = 11.84ALTCANCGQKK25 pKa = 10.37LHH27 pKa = 6.06VLEE30 pKa = 4.23IHH32 pKa = 6.15VCEE35 pKa = 4.35HH36 pKa = 6.64CCAEE40 pKa = 4.99LMSDD44 pKa = 4.35PNSSMYY50 pKa = 10.6EE51 pKa = 3.98EE52 pKa = 5.0EE53 pKa = 4.99DD54 pKa = 4.39DD55 pKa = 4.1EE56 pKa = 4.75

Molecular weight: 6.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZ24|Q9AZ24_BPHK6 Uncharacterized protein OS=Enterobacteria phage HK620 OX=155148 GN=hkaQ PE=4 SV=1
MM1 pKa = 7.07TVVITYY7 pKa = 9.95LADD10 pKa = 4.18DD11 pKa = 3.61NARR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84QAQRR25 pKa = 11.84EE26 pKa = 3.88QAMQEE31 pKa = 3.48QRR33 pKa = 11.84LARR36 pKa = 11.84KK37 pKa = 8.86IALKK41 pKa = 10.84LSGCVRR47 pKa = 11.84ADD49 pKa = 3.17KK50 pKa = 10.65AASLGSIRR58 pKa = 11.84CKK60 pKa = 10.38KK61 pKa = 10.64AEE63 pKa = 4.06EE64 pKa = 4.33CSGSICLPNVAIYY77 pKa = 10.18AAGYY81 pKa = 9.31RR82 pKa = 11.84KK83 pKa = 9.92SKK85 pKa = 10.61QLTARR90 pKa = 3.76

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11301

27

722

194.8

21.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.601 ± 0.588

1.203 ± 0.172

5.849 ± 0.259

6.451 ± 0.526

3.495 ± 0.25

7.274 ± 0.369

1.584 ± 0.164

6.53 ± 0.311

6.247 ± 0.392

7.778 ± 0.357

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.212

4.566 ± 0.328

3.734 ± 0.254

4.725 ± 0.418

5.619 ± 0.313

6.592 ± 0.338

5.115 ± 0.242

6.008 ± 0.346

1.195 ± 0.123

3.531 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski