Prosthecochloris aestuarii (strain DSM 271 / SK 413)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae; Prosthecochloris; Prosthecochloris aestuarii

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2319 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4S978|B4S978_PROA2 Uncharacterized protein OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) OX=290512 GN=Paes_0044 PE=4 SV=1
MM1 pKa = 6.9LTVWPIAYY9 pKa = 8.82MSGKK13 pKa = 10.44LGLTWMDD20 pKa = 3.21DD21 pKa = 3.96VEE23 pKa = 4.93FTSNWIDD30 pKa = 3.35STEE33 pKa = 4.07GFDD36 pKa = 4.22LGTDD40 pKa = 3.15AGVTLSCAVGYY51 pKa = 10.73DD52 pKa = 3.43FGDD55 pKa = 3.48YY56 pKa = 10.09RR57 pKa = 11.84AEE59 pKa = 4.29LEE61 pKa = 5.39LGYY64 pKa = 10.55QSNDD68 pKa = 2.31VDD70 pKa = 3.45TWADD74 pKa = 3.24SDD76 pKa = 4.14DD77 pKa = 4.98GYY79 pKa = 11.96VSDD82 pKa = 4.83AEE84 pKa = 4.28EE85 pKa = 4.11AGGEE89 pKa = 4.26VFVISVMAKK98 pKa = 8.86GAYY101 pKa = 10.02DD102 pKa = 3.38IDD104 pKa = 4.47LGGVEE109 pKa = 4.96LSPFVGAGFAFISFDD124 pKa = 3.66DD125 pKa = 4.51APVCFSGSEE134 pKa = 4.06YY135 pKa = 10.12RR136 pKa = 11.84SKK138 pKa = 11.06SATTFAYY145 pKa = 10.36QIGASLALPVADD157 pKa = 4.68TILLEE162 pKa = 3.79GRR164 pKa = 11.84YY165 pKa = 9.18RR166 pKa = 11.84YY167 pKa = 10.34LGTANFNFDD176 pKa = 3.65RR177 pKa = 11.84PEE179 pKa = 5.34AEE181 pKa = 4.62ADD183 pKa = 3.97FASHH187 pKa = 6.32SALVGLRR194 pKa = 11.84FSLL197 pKa = 4.19

Molecular weight: 21.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4S6Z1|B4S6Z1_PROA2 von Willebrand factor type A OS=Prosthecochloris aestuarii (strain DSM 271 / SK 413) OX=290512 GN=Paes_0782 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.6RR3 pKa = 11.84TFQPHH8 pKa = 3.66NRR10 pKa = 11.84KK11 pKa = 9.13RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 8.63HH16 pKa = 3.75GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.79NGRR28 pKa = 11.84RR29 pKa = 11.84ILASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84HH40 pKa = 4.91SLSVSSAMGTRR51 pKa = 11.84KK52 pKa = 8.38QQ53 pKa = 3.28

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2319

0

2319

748019

31

5009

322.6

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.391 ± 0.045

1.142 ± 0.023

5.713 ± 0.077

6.643 ± 0.047

4.286 ± 0.038

7.362 ± 0.047

2.204 ± 0.028

6.625 ± 0.05

5.02 ± 0.058

10.008 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.031

3.638 ± 0.031

4.19 ± 0.036

3.397 ± 0.032

5.799 ± 0.067

6.782 ± 0.037

5.149 ± 0.086

6.874 ± 0.051

1.071 ± 0.024

3.066 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski