Enterobacter phage CC31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Karamvirus; Escherichia virus CC31

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 279 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5DI84|E5DI84_9CAUD Uncharacterized protein 49.2 OS=Enterobacter phage CC31 OX=709484 GN=49.2 PE=4 SV=1
MM1 pKa = 7.71GYY3 pKa = 10.74ALKK6 pKa = 9.99PWYY9 pKa = 9.52AARR12 pKa = 11.84WEE14 pKa = 4.19TLEE17 pKa = 4.24PEE19 pKa = 4.15EE20 pKa = 4.6EE21 pKa = 4.09EE22 pKa = 5.02RR23 pKa = 11.84FPEE26 pKa = 4.81DD27 pKa = 5.18DD28 pKa = 3.76YY29 pKa = 12.26NEE31 pKa = 4.14PTNNEE36 pKa = 4.37LIDD39 pKa = 3.61MEE41 pKa = 4.62FGYY44 pKa = 10.29EE45 pKa = 3.95FPEE48 pKa = 4.14

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5DHZ3|E5DHZ3_9CAUD Uncharacterized protein OS=Enterobacter phage CC31 OX=709484 GN=CC31p261 PE=4 SV=1
MM1 pKa = 7.78AIAGPNIGTSWFRR14 pKa = 11.84EE15 pKa = 4.01TGQRR19 pKa = 11.84PMSAARR25 pKa = 11.84VAVRR29 pKa = 11.84LPARR33 pKa = 11.84PGGARR38 pKa = 11.84QMVGLSKK45 pKa = 10.37EE46 pKa = 3.89VNYY49 pKa = 10.99NIGANNSYY57 pKa = 11.32NKK59 pKa = 9.05DD60 pKa = 3.11TLINYY65 pKa = 7.63LRR67 pKa = 11.84SQGSTPVVVTITGNLVSYY85 pKa = 10.65SSGVACLEE93 pKa = 4.37FPANLPNAYY102 pKa = 9.4VHH104 pKa = 6.77LIINGGVTLYY114 pKa = 11.1GRR116 pKa = 11.84GGNGGVKK123 pKa = 10.58GNGAAGGHH131 pKa = 7.17AINNQFGTRR140 pKa = 11.84LRR142 pKa = 11.84ITNNGAIAGGGGGGGGNSANGGMGGGGRR170 pKa = 11.84PFGYY174 pKa = 10.3ADD176 pKa = 3.51KK177 pKa = 9.51THH179 pKa = 7.14PPAAATSRR187 pKa = 11.84AATDD191 pKa = 3.62GTLTSPGIGAEE202 pKa = 4.15YY203 pKa = 10.43KK204 pKa = 10.29IGTAVQYY211 pKa = 10.17TCGSGGNVGANGGASTGRR229 pKa = 11.84LGTNYY234 pKa = 10.49GGGSAGRR241 pKa = 11.84AVIGNAPTWNKK252 pKa = 9.99VGTIYY257 pKa = 10.88GSRR260 pKa = 11.84VV261 pKa = 2.97

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

279

0

279

52490

31

1264

188.1

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.064 ± 0.184

1.097 ± 0.081

6.207 ± 0.112

7.375 ± 0.175

4.344 ± 0.114

6.474 ± 0.2

1.846 ± 0.08

6.876 ± 0.107

7.331 ± 0.163

7.42 ± 0.136

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.894 ± 0.089

5.26 ± 0.139

3.604 ± 0.089

3.464 ± 0.086

4.586 ± 0.096

6.001 ± 0.123

5.843 ± 0.193

6.771 ± 0.122

1.515 ± 0.062

4.029 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski