Amino acid dipepetide frequency for Enterobacter phage CC31

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.944AlaAla: 5.944 ± 0.45
0.514AlaCys: 0.514 ± 0.109
3.677AlaAsp: 3.677 ± 0.232
5.373AlaGlu: 5.373 ± 0.344
2.515AlaPhe: 2.515 ± 0.244
5.22AlaGly: 5.22 ± 0.447
1.276AlaHis: 1.276 ± 0.18
4.763AlaIle: 4.763 ± 0.265
5.468AlaLys: 5.468 ± 0.299
6.077AlaLeu: 6.077 ± 0.353
1.6AlaMet: 1.6 ± 0.156
3.334AlaAsn: 3.334 ± 0.252
2.172AlaPro: 2.172 ± 0.223
2.629AlaGln: 2.629 ± 0.258
3.467AlaArg: 3.467 ± 0.251
4.058AlaSer: 4.058 ± 0.298
4.191AlaThr: 4.191 ± 0.387
4.725AlaVal: 4.725 ± 0.33
1.086AlaTrp: 1.086 ± 0.168
2.553AlaTyr: 2.553 ± 0.217
0.0AlaXaa: 0.0 ± 0.0
Cys
0.724CysAla: 0.724 ± 0.125
0.171CysCys: 0.171 ± 0.068
0.781CysAsp: 0.781 ± 0.118
0.914CysGlu: 0.914 ± 0.135
0.457CysPhe: 0.457 ± 0.11
0.724CysGly: 0.724 ± 0.124
0.267CysHis: 0.267 ± 0.074
0.591CysIle: 0.591 ± 0.119
0.61CysLys: 0.61 ± 0.119
0.686CysLeu: 0.686 ± 0.125
0.362CysMet: 0.362 ± 0.089
0.552CysAsn: 0.552 ± 0.112
0.533CysPro: 0.533 ± 0.095
0.343CysGln: 0.343 ± 0.078
0.61CysArg: 0.61 ± 0.109
0.667CysSer: 0.667 ± 0.109
0.629CysThr: 0.629 ± 0.116
0.61CysVal: 0.61 ± 0.111
0.21CysTrp: 0.21 ± 0.058
0.533CysTyr: 0.533 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
4.21AspAla: 4.21 ± 0.3
0.629AspCys: 0.629 ± 0.095
3.62AspAsp: 3.62 ± 0.27
5.125AspGlu: 5.125 ± 0.358
3.277AspPhe: 3.277 ± 0.26
4.896AspGly: 4.896 ± 0.306
0.914AspHis: 0.914 ± 0.144
4.649AspIle: 4.649 ± 0.304
4.306AspLys: 4.306 ± 0.349
4.706AspLeu: 4.706 ± 0.359
1.81AspMet: 1.81 ± 0.175
2.953AspAsn: 2.953 ± 0.235
2.134AspPro: 2.134 ± 0.195
1.619AspGln: 1.619 ± 0.189
1.905AspArg: 1.905 ± 0.193
3.544AspSer: 3.544 ± 0.246
3.296AspThr: 3.296 ± 0.274
4.172AspVal: 4.172 ± 0.259
1.143AspTrp: 1.143 ± 0.164
3.163AspTyr: 3.163 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
5.639GluAla: 5.639 ± 0.395
0.972GluCys: 0.972 ± 0.157
4.877GluAsp: 4.877 ± 0.365
5.563GluGlu: 5.563 ± 0.43
3.429GluPhe: 3.429 ± 0.294
3.906GluGly: 3.906 ± 0.264
1.562GluHis: 1.562 ± 0.187
5.354GluIle: 5.354 ± 0.308
4.725GluLys: 4.725 ± 0.332
6.382GluLeu: 6.382 ± 0.38
2.362GluMet: 2.362 ± 0.246
3.525GluAsn: 3.525 ± 0.274
2.191GluPro: 2.191 ± 0.205
2.343GluGln: 2.343 ± 0.19
3.067GluArg: 3.067 ± 0.235
4.039GluSer: 4.039 ± 0.248
4.039GluThr: 4.039 ± 0.322
5.582GluVal: 5.582 ± 0.335
1.124GluTrp: 1.124 ± 0.134
3.067GluTyr: 3.067 ± 0.245
0.0GluXaa: 0.0 ± 0.0
Phe
2.591PheAla: 2.591 ± 0.253
0.533PheCys: 0.533 ± 0.107
2.762PheAsp: 2.762 ± 0.235
3.715PheGlu: 3.715 ± 0.285
1.638PhePhe: 1.638 ± 0.182
3.01PheGly: 3.01 ± 0.237
0.819PheHis: 0.819 ± 0.132
2.877PheIle: 2.877 ± 0.253
4.077PheLys: 4.077 ± 0.251
2.496PheLeu: 2.496 ± 0.181
1.257PheMet: 1.257 ± 0.15
2.42PheAsn: 2.42 ± 0.208
1.467PhePro: 1.467 ± 0.16
1.486PheGln: 1.486 ± 0.172
1.81PheArg: 1.81 ± 0.187
2.667PheSer: 2.667 ± 0.213
2.572PheThr: 2.572 ± 0.232
2.915PheVal: 2.915 ± 0.234
0.686PheTrp: 0.686 ± 0.111
1.638PheTyr: 1.638 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
3.963GlyAla: 3.963 ± 0.349
0.781GlyCys: 0.781 ± 0.128
4.439GlyAsp: 4.439 ± 0.332
4.191GlyGlu: 4.191 ± 0.256
2.877GlyPhe: 2.877 ± 0.256
3.696GlyGly: 3.696 ± 0.437
1.143GlyHis: 1.143 ± 0.142
4.134GlyIle: 4.134 ± 0.254
4.458GlyLys: 4.458 ± 0.262
4.763GlyLeu: 4.763 ± 0.325
1.943GlyMet: 1.943 ± 0.234
3.448GlyAsn: 3.448 ± 0.36
1.81GlyPro: 1.81 ± 0.215
2.724GlyGln: 2.724 ± 0.279
2.801GlyArg: 2.801 ± 0.213
4.496GlySer: 4.496 ± 0.309
4.401GlyThr: 4.401 ± 0.358
4.591GlyVal: 4.591 ± 0.28
1.181GlyTrp: 1.181 ± 0.138
2.896GlyTyr: 2.896 ± 0.252
0.0GlyXaa: 0.0 ± 0.0
His
1.276HisAla: 1.276 ± 0.165
0.267HisCys: 0.267 ± 0.075
1.162HisAsp: 1.162 ± 0.16
0.895HisGlu: 0.895 ± 0.146
0.876HisPhe: 0.876 ± 0.117
1.257HisGly: 1.257 ± 0.152
0.457HisHis: 0.457 ± 0.109
1.467HisIle: 1.467 ± 0.226
1.276HisLys: 1.276 ± 0.19
1.524HisLeu: 1.524 ± 0.153
0.572HisMet: 0.572 ± 0.1
0.876HisAsn: 0.876 ± 0.144
1.143HisPro: 1.143 ± 0.14
0.724HisGln: 0.724 ± 0.128
0.629HisArg: 0.629 ± 0.107
0.8HisSer: 0.8 ± 0.119
0.876HisThr: 0.876 ± 0.134
1.296HisVal: 1.296 ± 0.128
0.229HisTrp: 0.229 ± 0.066
0.857HisTyr: 0.857 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
5.011IleAla: 5.011 ± 0.407
0.876IleCys: 0.876 ± 0.133
4.706IleAsp: 4.706 ± 0.324
5.525IleGlu: 5.525 ± 0.278
2.496IlePhe: 2.496 ± 0.239
3.544IleGly: 3.544 ± 0.267
1.276IleHis: 1.276 ± 0.133
4.61IleIle: 4.61 ± 0.267
5.296IleLys: 5.296 ± 0.324
3.848IleLeu: 3.848 ± 0.292
2.039IleMet: 2.039 ± 0.202
4.382IleAsn: 4.382 ± 0.333
2.82IlePro: 2.82 ± 0.235
2.172IleGln: 2.172 ± 0.199
3.772IleArg: 3.772 ± 0.269
3.925IleSer: 3.925 ± 0.334
4.496IleThr: 4.496 ± 0.311
4.591IleVal: 4.591 ± 0.29
0.914IleTrp: 0.914 ± 0.155
2.458IleTyr: 2.458 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
5.792LysAla: 5.792 ± 0.352
0.934LysCys: 0.934 ± 0.134
4.363LysAsp: 4.363 ± 0.261
5.334LysGlu: 5.334 ± 0.348
3.391LysPhe: 3.391 ± 0.25
4.287LysGly: 4.287 ± 0.272
1.448LysHis: 1.448 ± 0.18
4.934LysIle: 4.934 ± 0.296
4.344LysLys: 4.344 ± 0.298
6.077LysLeu: 6.077 ± 0.391
2.705LysMet: 2.705 ± 0.229
3.544LysAsn: 3.544 ± 0.273
2.229LysPro: 2.229 ± 0.227
2.553LysGln: 2.553 ± 0.215
3.677LysArg: 3.677 ± 0.298
4.21LysSer: 4.21 ± 0.335
4.229LysThr: 4.229 ± 0.264
4.992LysVal: 4.992 ± 0.381
1.143LysTrp: 1.143 ± 0.139
3.124LysTyr: 3.124 ± 0.264
0.0LysXaa: 0.0 ± 0.0
Leu
5.449LeuAla: 5.449 ± 0.362
0.762LeuCys: 0.762 ± 0.126
5.106LeuAsp: 5.106 ± 0.366
5.62LeuGlu: 5.62 ± 0.354
3.067LeuPhe: 3.067 ± 0.25
4.401LeuGly: 4.401 ± 0.303
1.315LeuHis: 1.315 ± 0.157
4.572LeuIle: 4.572 ± 0.323
6.001LeuLys: 6.001 ± 0.34
4.553LeuLeu: 4.553 ± 0.293
2.019LeuMet: 2.019 ± 0.19
4.553LeuAsn: 4.553 ± 0.309
3.201LeuPro: 3.201 ± 0.243
2.61LeuGln: 2.61 ± 0.259
3.791LeuArg: 3.791 ± 0.304
4.591LeuSer: 4.591 ± 0.33
4.172LeuThr: 4.172 ± 0.285
4.63LeuVal: 4.63 ± 0.312
0.914LeuTrp: 0.914 ± 0.126
2.858LeuTyr: 2.858 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
2.058MetAla: 2.058 ± 0.179
0.229MetCys: 0.229 ± 0.068
1.238MetAsp: 1.238 ± 0.171
1.848MetGlu: 1.848 ± 0.207
1.467MetPhe: 1.467 ± 0.165
1.41MetGly: 1.41 ± 0.177
0.438MetHis: 0.438 ± 0.107
1.81MetIle: 1.81 ± 0.197
3.029MetLys: 3.029 ± 0.262
2.305MetLeu: 2.305 ± 0.215
1.029MetMet: 1.029 ± 0.15
2.019MetAsn: 2.019 ± 0.185
0.819MetPro: 0.819 ± 0.107
1.067MetGln: 1.067 ± 0.137
1.162MetArg: 1.162 ± 0.142
1.943MetSer: 1.943 ± 0.202
2.115MetThr: 2.115 ± 0.188
1.81MetVal: 1.81 ± 0.183
0.286MetTrp: 0.286 ± 0.077
0.857MetTyr: 0.857 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.429AsnAla: 3.429 ± 0.293
0.552AsnCys: 0.552 ± 0.091
3.086AsnAsp: 3.086 ± 0.234
3.715AsnGlu: 3.715 ± 0.292
2.343AsnPhe: 2.343 ± 0.236
4.839AsnGly: 4.839 ± 0.392
0.838AsnHis: 0.838 ± 0.132
3.715AsnIle: 3.715 ± 0.252
3.829AsnLys: 3.829 ± 0.24
3.62AsnLeu: 3.62 ± 0.235
1.257AsnMet: 1.257 ± 0.197
2.82AsnAsn: 2.82 ± 0.257
2.248AsnPro: 2.248 ± 0.214
1.81AsnGln: 1.81 ± 0.161
2.782AsnArg: 2.782 ± 0.23
3.105AsnSer: 3.105 ± 0.254
2.705AsnThr: 2.705 ± 0.263
3.22AsnVal: 3.22 ± 0.242
0.705AsnTrp: 0.705 ± 0.129
1.981AsnTyr: 1.981 ± 0.23
0.0AsnXaa: 0.0 ± 0.0
Pro
2.439ProAla: 2.439 ± 0.231
0.476ProCys: 0.476 ± 0.1
2.553ProAsp: 2.553 ± 0.24
3.277ProGlu: 3.277 ± 0.281
1.619ProPhe: 1.619 ± 0.174
2.553ProGly: 2.553 ± 0.19
0.705ProHis: 0.705 ± 0.104
2.21ProIle: 2.21 ± 0.208
2.401ProLys: 2.401 ± 0.3
2.21ProLeu: 2.21 ± 0.173
0.857ProMet: 0.857 ± 0.136
1.772ProAsn: 1.772 ± 0.171
0.991ProPro: 0.991 ± 0.177
1.086ProGln: 1.086 ± 0.153
1.581ProArg: 1.581 ± 0.19
2.42ProSer: 2.42 ± 0.188
2.305ProThr: 2.305 ± 0.193
2.458ProVal: 2.458 ± 0.236
0.876ProTrp: 0.876 ± 0.134
1.257ProTyr: 1.257 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
2.248GlnAla: 2.248 ± 0.253
0.267GlnCys: 0.267 ± 0.074
1.543GlnAsp: 1.543 ± 0.149
2.229GlnGlu: 2.229 ± 0.192
1.753GlnPhe: 1.753 ± 0.216
2.039GlnGly: 2.039 ± 0.188
0.61GlnHis: 0.61 ± 0.12
2.877GlnIle: 2.877 ± 0.216
2.286GlnLys: 2.286 ± 0.189
3.353GlnLeu: 3.353 ± 0.253
1.143GlnMet: 1.143 ± 0.144
1.543GlnAsn: 1.543 ± 0.164
1.162GlnPro: 1.162 ± 0.167
1.181GlnGln: 1.181 ± 0.135
1.772GlnArg: 1.772 ± 0.186
1.981GlnSer: 1.981 ± 0.184
2.019GlnThr: 2.019 ± 0.173
2.496GlnVal: 2.496 ± 0.22
0.495GlnTrp: 0.495 ± 0.106
1.638GlnTyr: 1.638 ± 0.188
0.0GlnXaa: 0.0 ± 0.0
Arg
3.01ArgAla: 3.01 ± 0.275
0.533ArgCys: 0.533 ± 0.098
2.61ArgAsp: 2.61 ± 0.187
3.372ArgGlu: 3.372 ± 0.282
1.981ArgPhe: 1.981 ± 0.192
2.953ArgGly: 2.953 ± 0.259
0.972ArgHis: 0.972 ± 0.148
3.486ArgIle: 3.486 ± 0.253
3.601ArgLys: 3.601 ± 0.274
3.658ArgLeu: 3.658 ± 0.278
1.581ArgMet: 1.581 ± 0.143
2.229ArgAsn: 2.229 ± 0.207
1.657ArgPro: 1.657 ± 0.165
1.791ArgGln: 1.791 ± 0.202
2.115ArgArg: 2.115 ± 0.201
2.534ArgSer: 2.534 ± 0.243
2.381ArgThr: 2.381 ± 0.203
3.144ArgVal: 3.144 ± 0.24
0.667ArgTrp: 0.667 ± 0.113
1.581ArgTyr: 1.581 ± 0.211
0.0ArgXaa: 0.0 ± 0.0
Ser
4.134SerAla: 4.134 ± 0.293
0.667SerCys: 0.667 ± 0.112
3.486SerAsp: 3.486 ± 0.229
4.039SerGlu: 4.039 ± 0.285
2.705SerPhe: 2.705 ± 0.228
4.782SerGly: 4.782 ± 0.348
0.762SerHis: 0.762 ± 0.136
4.21SerIle: 4.21 ± 0.28
4.61SerLys: 4.61 ± 0.275
4.134SerLeu: 4.134 ± 0.289
1.657SerMet: 1.657 ± 0.166
2.858SerAsn: 2.858 ± 0.263
2.381SerPro: 2.381 ± 0.237
2.058SerGln: 2.058 ± 0.195
2.972SerArg: 2.972 ± 0.218
3.791SerSer: 3.791 ± 0.281
3.505SerThr: 3.505 ± 0.277
4.001SerVal: 4.001 ± 0.27
0.838SerTrp: 0.838 ± 0.115
2.42SerTyr: 2.42 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
4.096ThrAla: 4.096 ± 0.345
0.572ThrCys: 0.572 ± 0.109
3.029ThrAsp: 3.029 ± 0.308
3.925ThrGlu: 3.925 ± 0.276
2.61ThrPhe: 2.61 ± 0.23
4.61ThrGly: 4.61 ± 0.32
1.067ThrHis: 1.067 ± 0.142
4.153ThrIle: 4.153 ± 0.292
3.734ThrLys: 3.734 ± 0.246
4.934ThrLeu: 4.934 ± 0.4
1.296ThrMet: 1.296 ± 0.144
2.858ThrAsn: 2.858 ± 0.226
3.124ThrPro: 3.124 ± 0.292
1.943ThrGln: 1.943 ± 0.25
2.572ThrArg: 2.572 ± 0.232
3.963ThrSer: 3.963 ± 0.297
3.01ThrThr: 3.01 ± 0.258
3.848ThrVal: 3.848 ± 0.307
0.857ThrTrp: 0.857 ± 0.14
2.229ThrTyr: 2.229 ± 0.235
0.0ThrXaa: 0.0 ± 0.0
Val
4.687ValAla: 4.687 ± 0.283
0.591ValCys: 0.591 ± 0.104
4.858ValAsp: 4.858 ± 0.306
5.03ValGlu: 5.03 ± 0.315
2.782ValPhe: 2.782 ± 0.266
3.201ValGly: 3.201 ± 0.255
1.391ValHis: 1.391 ± 0.17
4.572ValIle: 4.572 ± 0.321
5.449ValLys: 5.449 ± 0.319
4.877ValLeu: 4.877 ± 0.281
1.848ValMet: 1.848 ± 0.186
3.753ValAsn: 3.753 ± 0.282
2.191ValPro: 2.191 ± 0.233
2.686ValGln: 2.686 ± 0.237
2.934ValArg: 2.934 ± 0.24
4.096ValSer: 4.096 ± 0.251
4.134ValThr: 4.134 ± 0.294
4.744ValVal: 4.744 ± 0.308
0.953ValTrp: 0.953 ± 0.135
2.934ValTyr: 2.934 ± 0.24
0.0ValXaa: 0.0 ± 0.0
Trp
0.762TrpAla: 0.762 ± 0.097
0.229TrpCys: 0.229 ± 0.066
1.067TrpAsp: 1.067 ± 0.145
1.124TrpGlu: 1.124 ± 0.147
0.667TrpPhe: 0.667 ± 0.113
0.8TrpGly: 0.8 ± 0.111
0.324TrpHis: 0.324 ± 0.096
0.972TrpIle: 0.972 ± 0.134
1.467TrpLys: 1.467 ± 0.166
1.2TrpLeu: 1.2 ± 0.152
0.572TrpMet: 0.572 ± 0.109
0.781TrpAsn: 0.781 ± 0.136
0.4TrpPro: 0.4 ± 0.087
0.476TrpGln: 0.476 ± 0.091
0.705TrpArg: 0.705 ± 0.124
0.8TrpSer: 0.8 ± 0.11
0.857TrpThr: 0.857 ± 0.142
0.914TrpVal: 0.914 ± 0.12
0.267TrpTrp: 0.267 ± 0.071
0.762TrpTyr: 0.762 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.182TyrAla: 3.182 ± 0.225
0.419TyrCys: 0.419 ± 0.09
3.105TyrAsp: 3.105 ± 0.254
2.934TyrGlu: 2.934 ± 0.239
1.486TyrPhe: 1.486 ± 0.167
2.61TyrGly: 2.61 ± 0.239
0.876TyrHis: 0.876 ± 0.139
2.801TyrIle: 2.801 ± 0.245
2.343TyrLys: 2.343 ± 0.211
2.801TyrLeu: 2.801 ± 0.229
1.01TyrMet: 1.01 ± 0.139
2.381TyrAsn: 2.381 ± 0.2
1.372TyrPro: 1.372 ± 0.153
1.334TyrGln: 1.334 ± 0.161
1.734TyrArg: 1.734 ± 0.185
2.381TyrSer: 2.381 ± 0.222
2.496TyrThr: 2.496 ± 0.225
2.972TyrVal: 2.972 ± 0.257
0.572TyrTrp: 0.572 ± 0.088
1.486TyrTyr: 1.486 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 279 proteins (52490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski