Hyalangium minutum
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8967 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A085VZS1|A0A085VZS1_9DELT Chromosome partition protein smc OS=Hyalangium minutum OX=394096 GN=DB31_4558 PE=4 SV=1
MM1 pKa = 7.52 RR2 pKa = 11.84 RR3 pKa = 11.84 SSSSSRR9 pKa = 11.84 ISAVCSSLLAALGLLLSAPASAQWTPGTALPHH41 pKa = 6.18 AHH43 pKa = 5.55 TQGVIIPDD51 pKa = 3.28 ATSRR55 pKa = 11.84 IHH57 pKa = 7.3 AIGGGDD63 pKa = 3.39 SGAFDD68 pKa = 3.99 TNAHH72 pKa = 6.76 DD73 pKa = 3.97 EE74 pKa = 4.4 FDD76 pKa = 4.43 PVTHH80 pKa = 5.98 TWITRR85 pKa = 11.84 APLPINNRR93 pKa = 11.84 GMGASLGPDD102 pKa = 2.96 GRR104 pKa = 11.84 IYY106 pKa = 10.88 VYY108 pKa = 10.9 GGFAGWGSIGAVYY121 pKa = 10.03 AYY123 pKa = 8.93 TPSTDD128 pKa = 2.46 TWAPLASMPTPQWEE142 pKa = 4.23 TRR144 pKa = 11.84 GDD146 pKa = 3.52 FGSDD150 pKa = 2.39 GKK152 pKa = 11.28 LYY154 pKa = 11.03 VFGGEE159 pKa = 4.54 TNGSASAVTQIYY171 pKa = 8.34 TPSTNTWTTGASMPGGRR188 pKa = 11.84 RR189 pKa = 11.84 QHH191 pKa = 5.56 GAFRR195 pKa = 11.84 GSDD198 pKa = 2.83 GRR200 pKa = 11.84 FYY202 pKa = 11.57 VVGGQDD208 pKa = 3.03 ASAATAGTYY217 pKa = 9.6 IYY219 pKa = 11.06 NPTTNTWTTGPAMPAAANSFGQAVSTDD246 pKa = 3.04 RR247 pKa = 11.84 SRR249 pKa = 11.84 FFVLGGSTSYY259 pKa = 11.47 NNEE262 pKa = 3.83 STPLFDD268 pKa = 3.15 TVYY271 pKa = 9.07 MFNVNTQEE279 pKa = 4.04 WTSLTGFPTARR290 pKa = 11.84 RR291 pKa = 11.84 EE292 pKa = 3.88 LGATVTHH299 pKa = 6.38 CRR301 pKa = 11.84 LRR303 pKa = 11.84 TLGGSTGTSVTTHH316 pKa = 5.7 EE317 pKa = 4.44 VFTLTGAPDD326 pKa = 3.74 TNGNGQPDD334 pKa = 4.14 YY335 pKa = 11.59 CEE337 pKa = 5.71 DD338 pKa = 4.57 PDD340 pKa = 4.96 IDD342 pKa = 5.35 DD343 pKa = 5.72 DD344 pKa = 4.53 NVPNSADD351 pKa = 3.43 NCPTVPNPGQEE362 pKa = 4.28 NQDD365 pKa = 3.19 HH366 pKa = 7.17 DD367 pKa = 4.43 AQGDD371 pKa = 3.81 ACDD374 pKa = 4.35 PDD376 pKa = 4.72 ADD378 pKa = 4.93 NDD380 pKa = 4.18 ALPNGQDD387 pKa = 3.29 NCPLHH392 pKa = 6.88 PNADD396 pKa = 3.84 QTDD399 pKa = 3.57 SDD401 pKa = 4.76 ADD403 pKa = 3.9 GLGNACDD410 pKa = 4.4 PDD412 pKa = 3.74 NDD414 pKa = 3.99 NDD416 pKa = 3.93 GTADD420 pKa = 4.44 LSDD423 pKa = 3.81 NCPSQSNADD432 pKa = 3.52 QHH434 pKa = 6.67 DD435 pKa = 3.81 TDD437 pKa = 4.7 GDD439 pKa = 4.53 SIGDD443 pKa = 3.72 ACDD446 pKa = 3.9 SDD448 pKa = 5.21 DD449 pKa = 5.72 DD450 pKa = 4.61 NDD452 pKa = 5.47 SIADD456 pKa = 3.84 PQDD459 pKa = 3.39 NCPLVANTDD468 pKa = 3.38 QRR470 pKa = 11.84 DD471 pKa = 3.48 TDD473 pKa = 3.73 GDD475 pKa = 4.53 SIGDD479 pKa = 3.76 ACDD482 pKa = 3.6 SDD484 pKa = 3.93 TDD486 pKa = 4.01 SDD488 pKa = 4.09 NDD490 pKa = 4.02 TVPDD494 pKa = 3.72 VTDD497 pKa = 3.32 NCALVPNPDD506 pKa = 3.07 QANADD511 pKa = 3.67 KK512 pKa = 11.14 DD513 pKa = 4.17 EE514 pKa = 4.68 QGDD517 pKa = 3.87 ACDD520 pKa = 5.24 SDD522 pKa = 4.83 DD523 pKa = 6.31 DD524 pKa = 4.62 NDD526 pKa = 4.03 TVADD530 pKa = 3.89 EE531 pKa = 4.35 WDD533 pKa = 3.63 NCPFIPNTDD542 pKa = 3.43 QADD545 pKa = 3.43 SDD547 pKa = 4.52 GNGKK551 pKa = 10.56 GDD553 pKa = 3.53 VCDD556 pKa = 4.21 VDD558 pKa = 4.7 NDD560 pKa = 3.73 TDD562 pKa = 4.15 KK563 pKa = 11.81 DD564 pKa = 3.92 GVANATDD571 pKa = 3.73 NCPLVANPDD580 pKa = 3.71 QLDD583 pKa = 3.52 SDD585 pKa = 4.24 GDD587 pKa = 4.4 GVGDD591 pKa = 4.02 ACDD594 pKa = 3.6 EE595 pKa = 4.49 SGGGAGGCGGGCGAGPIGSSSLMLWALAGLALRR628 pKa = 11.84 ARR630 pKa = 11.84 RR631 pKa = 11.84 RR632 pKa = 11.84 FARR635 pKa = 3.78
Molecular weight: 65.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.661
IPC2_protein 3.643
IPC_protein 3.719
Toseland 3.465
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.719
Rodwell 3.528
Grimsley 3.363
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.19
Thurlkill 3.528
EMBOSS 3.719
Sillero 3.846
Patrickios 1.278
IPC_peptide 3.719
IPC2_peptide 3.808
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A085WNG5|A0A085WNG5_9DELT Uncharacterized protein OS=Hyalangium minutum OX=394096 GN=DB31_7130 PE=4 SV=1
MM1 pKa = 7.07 VRR3 pKa = 11.84 GHH5 pKa = 5.61 STSGFHH11 pKa = 7.19 GNRR14 pKa = 11.84 PSSRR18 pKa = 11.84 FWPARR23 pKa = 11.84 GALGQALHH31 pKa = 6.76 GSAAAAPAQGAAGRR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 6.16 LSLSALRR54 pKa = 11.84 SVLNAHH60 pKa = 7.28 LSPLSSRR67 pKa = 11.84 SSPSMAPARR76 pKa = 11.84 PQSPPAHH83 pKa = 6.34 RR84 pKa = 4.59
Molecular weight: 8.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.466
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8967
0
8967
3319859
37
10488
370.2
40.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.534 ± 0.037
0.944 ± 0.012
4.794 ± 0.02
6.591 ± 0.036
3.387 ± 0.018
8.568 ± 0.032
2.043 ± 0.012
3.239 ± 0.017
3.263 ± 0.027
10.931 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.946 ± 0.012
2.333 ± 0.021
6.215 ± 0.027
3.772 ± 0.016
7.472 ± 0.034
6.055 ± 0.027
5.592 ± 0.039
7.778 ± 0.028
1.358 ± 0.012
2.188 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here