Phenylobacterium deserti

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3698 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328AV49|A0A328AV49_9CAUL Uncharacterized protein OS=Phenylobacterium deserti OX=1914756 GN=DJ018_02150 PE=4 SV=1
MM1 pKa = 7.12FASAHH6 pKa = 5.83NGAGLALNIARR17 pKa = 11.84EE18 pKa = 4.29EE19 pKa = 4.24TNYY22 pKa = 9.21MRR24 pKa = 11.84PNSMDD29 pKa = 3.1DD30 pKa = 3.24FARR33 pKa = 11.84ITRR36 pKa = 11.84GTRR39 pKa = 11.84DD40 pKa = 3.37AQKK43 pKa = 10.92PEE45 pKa = 3.89ATLSGLTPTSATFTHH60 pKa = 7.26ADD62 pKa = 3.27GAAPATTVGSLGTEE76 pKa = 4.01RR77 pKa = 11.84YY78 pKa = 10.13DD79 pKa = 4.7LIPVALTAGTTYY91 pKa = 11.03SFAYY95 pKa = 10.14RR96 pKa = 11.84PTEE99 pKa = 3.67TGGIEE104 pKa = 4.42DD105 pKa = 4.48PFLGLVNPAGTQFLAQDD122 pKa = 3.98DD123 pKa = 4.37DD124 pKa = 4.72GGLGRR129 pKa = 11.84SSMITFTPTASGTYY143 pKa = 10.55LLYY146 pKa = 7.97TTSWYY151 pKa = 10.52HH152 pKa = 6.64IDD154 pKa = 3.76PSAPGYY160 pKa = 9.76PNYY163 pKa = 9.98RR164 pKa = 11.84DD165 pKa = 3.31AGGYY169 pKa = 7.2TVDD172 pKa = 3.85MWTADD177 pKa = 3.85PATDD181 pKa = 4.07APSTRR186 pKa = 11.84AGALTIDD193 pKa = 3.08VGTTYY198 pKa = 11.07GHH200 pKa = 7.46LDD202 pKa = 3.22AAGDD206 pKa = 3.38QDD208 pKa = 3.93MYY210 pKa = 10.85RR211 pKa = 11.84VEE213 pKa = 4.7LDD215 pKa = 2.99PGLFYY220 pKa = 10.86TFTYY224 pKa = 10.39AGGIASSAEE233 pKa = 4.08YY234 pKa = 9.82PGEE237 pKa = 4.02LPGEE241 pKa = 4.44SIGVLRR247 pKa = 11.84LYY249 pKa = 10.44DD250 pKa = 3.58AEE252 pKa = 4.34GNEE255 pKa = 4.07LSAAVNYY262 pKa = 7.47EE263 pKa = 3.86TGLGFLSPEE272 pKa = 3.8GGTYY276 pKa = 8.44YY277 pKa = 10.79LRR279 pKa = 11.84VEE281 pKa = 4.63GYY283 pKa = 9.87EE284 pKa = 3.83PDD286 pKa = 3.29MTGGYY291 pKa = 7.72TLDD294 pKa = 4.36VKK296 pKa = 11.14AQDD299 pKa = 3.91PTDD302 pKa = 4.15FDD304 pKa = 4.49PLEE307 pKa = 4.19SLNWDD312 pKa = 3.52EE313 pKa = 5.39ADD315 pKa = 4.33NIPTTMVNGVPTAYY329 pKa = 10.51VYY331 pKa = 10.06FASARR336 pKa = 11.84EE337 pKa = 4.12GGFGEE342 pKa = 4.5TEE344 pKa = 4.23DD345 pKa = 5.76DD346 pKa = 3.98GVTPITTYY354 pKa = 10.46GWEE357 pKa = 3.82QFQIQGVMRR366 pKa = 11.84ALQEE370 pKa = 3.97YY371 pKa = 8.92TPITGINYY379 pKa = 8.71VRR381 pKa = 11.84TTDD384 pKa = 3.06INQATFRR391 pKa = 11.84LVTTINDD398 pKa = 3.48DD399 pKa = 3.28YY400 pKa = 11.5GARR403 pKa = 11.84FNPRR407 pKa = 11.84DD408 pKa = 3.63PSAGDD413 pKa = 3.44LQGVGVFNLASGGFTRR429 pKa = 11.84PEE431 pKa = 3.8TLEE434 pKa = 4.17PGGYY438 pKa = 9.13SYY440 pKa = 11.74AVVLHH445 pKa = 6.1EE446 pKa = 5.07FGHH449 pKa = 5.58AHH451 pKa = 6.27GVAHH455 pKa = 6.5PHH457 pKa = 6.86DD458 pKa = 4.48EE459 pKa = 4.64GGGSEE464 pKa = 4.12ILLGVTGPTGSLGIYY479 pKa = 9.9DD480 pKa = 4.96LNQGVYY486 pKa = 8.91TVMSYY491 pKa = 11.59NDD493 pKa = 3.39GWQTNPDD500 pKa = 3.38GPLEE504 pKa = 4.16FTRR507 pKa = 11.84QTWGSGWSGTLGAFDD522 pKa = 3.87IAVLQARR529 pKa = 11.84YY530 pKa = 9.16GVHH533 pKa = 6.14AHH535 pKa = 5.16NTGDD539 pKa = 3.4NVYY542 pKa = 11.03ALTGPQKK549 pKa = 10.42DD550 pKa = 4.03AYY552 pKa = 10.0YY553 pKa = 8.82QTIWDD558 pKa = 3.78TGGNDD563 pKa = 3.48TIAYY567 pKa = 9.86DD568 pKa = 3.97GGRR571 pKa = 11.84DD572 pKa = 3.41AHH574 pKa = 7.02IDD576 pKa = 3.65LLAATLDD583 pKa = 3.77YY584 pKa = 10.42TPTGGGVVSFVDD596 pKa = 4.0GTYY599 pKa = 10.93GGYY602 pKa = 9.01TIANGVVIEE611 pKa = 4.15NARR614 pKa = 11.84GGNGSDD620 pKa = 2.97ALMGNSVANRR630 pKa = 11.84LDD632 pKa = 3.58GQNGDD637 pKa = 4.02DD638 pKa = 3.8GLLGRR643 pKa = 11.84EE644 pKa = 4.4GDD646 pKa = 3.98DD647 pKa = 4.82LLIGGNGKK655 pKa = 8.44DD656 pKa = 3.62TLNGGTGNDD665 pKa = 3.86SLDD668 pKa = 3.56GGTGRR673 pKa = 11.84DD674 pKa = 3.77VLNGGAGDD682 pKa = 3.78DD683 pKa = 3.86TLNGGLHH690 pKa = 7.34DD691 pKa = 4.84DD692 pKa = 4.11LFVFEE697 pKa = 5.59DD698 pKa = 3.86AGTDD702 pKa = 3.69TIVGYY707 pKa = 10.23QKK709 pKa = 11.15GEE711 pKa = 4.03QFDD714 pKa = 4.56LSALNVTWADD724 pKa = 3.36VTIEE728 pKa = 4.0SGRR731 pKa = 11.84IGVDD735 pKa = 3.38LEE737 pKa = 4.2GDD739 pKa = 3.21QDD741 pKa = 3.61LTIIVNTSGITQGNFIFAA759 pKa = 4.52

Molecular weight:
80.2 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328AU99|A0A328AU99_9CAUL Beta-ketoacyl-ACP reductase OS=Phenylobacterium deserti OX=1914756 GN=DJ018_08010 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.47GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTKK25 pKa = 10.02NGQKK29 pKa = 9.43ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.28 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3698

0

3698

1169876

27

1930

316.4

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.846 ± 0.065

0.722 ± 0.011

5.54 ± 0.028

5.902 ± 0.046

3.536 ± 0.025

8.937 ± 0.039

1.778 ± 0.02

4.071 ± 0.027

2.858 ± 0.03

10.116 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.019

2.367 ± 0.03

5.655 ± 0.035

3.482 ± 0.024

7.729 ± 0.046

5.133 ± 0.033

5.061 ± 0.033

7.58 ± 0.034

1.438 ± 0.018

2.104 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski