Phenylobacterium deserti
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3698 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328AV49|A0A328AV49_9CAUL Uncharacterized protein OS=Phenylobacterium deserti OX=1914756 GN=DJ018_02150 PE=4 SV=1
MM1 pKa = 7.12 FASAHH6 pKa = 5.83 NGAGLALNIARR17 pKa = 11.84 EE18 pKa = 4.29 EE19 pKa = 4.24 TNYY22 pKa = 9.21 MRR24 pKa = 11.84 PNSMDD29 pKa = 3.1 DD30 pKa = 3.24 FARR33 pKa = 11.84 ITRR36 pKa = 11.84 GTRR39 pKa = 11.84 DD40 pKa = 3.37 AQKK43 pKa = 10.92 PEE45 pKa = 3.89 ATLSGLTPTSATFTHH60 pKa = 7.26 ADD62 pKa = 3.27 GAAPATTVGSLGTEE76 pKa = 4.01 RR77 pKa = 11.84 YY78 pKa = 10.13 DD79 pKa = 4.7 LIPVALTAGTTYY91 pKa = 11.03 SFAYY95 pKa = 10.14 RR96 pKa = 11.84 PTEE99 pKa = 3.67 TGGIEE104 pKa = 4.42 DD105 pKa = 4.48 PFLGLVNPAGTQFLAQDD122 pKa = 3.98 DD123 pKa = 4.37 DD124 pKa = 4.72 GGLGRR129 pKa = 11.84 SSMITFTPTASGTYY143 pKa = 10.55 LLYY146 pKa = 7.97 TTSWYY151 pKa = 10.52 HH152 pKa = 6.64 IDD154 pKa = 3.76 PSAPGYY160 pKa = 9.76 PNYY163 pKa = 9.98 RR164 pKa = 11.84 DD165 pKa = 3.31 AGGYY169 pKa = 7.2 TVDD172 pKa = 3.85 MWTADD177 pKa = 3.85 PATDD181 pKa = 4.07 APSTRR186 pKa = 11.84 AGALTIDD193 pKa = 3.08 VGTTYY198 pKa = 11.07 GHH200 pKa = 7.46 LDD202 pKa = 3.22 AAGDD206 pKa = 3.38 QDD208 pKa = 3.93 MYY210 pKa = 10.85 RR211 pKa = 11.84 VEE213 pKa = 4.7 LDD215 pKa = 2.99 PGLFYY220 pKa = 10.86 TFTYY224 pKa = 10.39 AGGIASSAEE233 pKa = 4.08 YY234 pKa = 9.82 PGEE237 pKa = 4.02 LPGEE241 pKa = 4.44 SIGVLRR247 pKa = 11.84 LYY249 pKa = 10.44 DD250 pKa = 3.58 AEE252 pKa = 4.34 GNEE255 pKa = 4.07 LSAAVNYY262 pKa = 7.47 EE263 pKa = 3.86 TGLGFLSPEE272 pKa = 3.8 GGTYY276 pKa = 8.44 YY277 pKa = 10.79 LRR279 pKa = 11.84 VEE281 pKa = 4.63 GYY283 pKa = 9.87 EE284 pKa = 3.83 PDD286 pKa = 3.29 MTGGYY291 pKa = 7.72 TLDD294 pKa = 4.36 VKK296 pKa = 11.14 AQDD299 pKa = 3.91 PTDD302 pKa = 4.15 FDD304 pKa = 4.49 PLEE307 pKa = 4.19 SLNWDD312 pKa = 3.52 EE313 pKa = 5.39 ADD315 pKa = 4.33 NIPTTMVNGVPTAYY329 pKa = 10.51 VYY331 pKa = 10.06 FASARR336 pKa = 11.84 EE337 pKa = 4.12 GGFGEE342 pKa = 4.5 TEE344 pKa = 4.23 DD345 pKa = 5.76 DD346 pKa = 3.98 GVTPITTYY354 pKa = 10.46 GWEE357 pKa = 3.82 QFQIQGVMRR366 pKa = 11.84 ALQEE370 pKa = 3.97 YY371 pKa = 8.92 TPITGINYY379 pKa = 8.71 VRR381 pKa = 11.84 TTDD384 pKa = 3.06 INQATFRR391 pKa = 11.84 LVTTINDD398 pKa = 3.48 DD399 pKa = 3.28 YY400 pKa = 11.5 GARR403 pKa = 11.84 FNPRR407 pKa = 11.84 DD408 pKa = 3.63 PSAGDD413 pKa = 3.44 LQGVGVFNLASGGFTRR429 pKa = 11.84 PEE431 pKa = 3.8 TLEE434 pKa = 4.17 PGGYY438 pKa = 9.13 SYY440 pKa = 11.74 AVVLHH445 pKa = 6.1 EE446 pKa = 5.07 FGHH449 pKa = 5.58 AHH451 pKa = 6.27 GVAHH455 pKa = 6.5 PHH457 pKa = 6.86 DD458 pKa = 4.48 EE459 pKa = 4.64 GGGSEE464 pKa = 4.12 ILLGVTGPTGSLGIYY479 pKa = 9.9 DD480 pKa = 4.96 LNQGVYY486 pKa = 8.91 TVMSYY491 pKa = 11.59 NDD493 pKa = 3.39 GWQTNPDD500 pKa = 3.38 GPLEE504 pKa = 4.16 FTRR507 pKa = 11.84 QTWGSGWSGTLGAFDD522 pKa = 3.87 IAVLQARR529 pKa = 11.84 YY530 pKa = 9.16 GVHH533 pKa = 6.14 AHH535 pKa = 5.16 NTGDD539 pKa = 3.4 NVYY542 pKa = 11.03 ALTGPQKK549 pKa = 10.42 DD550 pKa = 4.03 AYY552 pKa = 10.0 YY553 pKa = 8.82 QTIWDD558 pKa = 3.78 TGGNDD563 pKa = 3.48 TIAYY567 pKa = 9.86 DD568 pKa = 3.97 GGRR571 pKa = 11.84 DD572 pKa = 3.41 AHH574 pKa = 7.02 IDD576 pKa = 3.65 LLAATLDD583 pKa = 3.77 YY584 pKa = 10.42 TPTGGGVVSFVDD596 pKa = 4.0 GTYY599 pKa = 10.93 GGYY602 pKa = 9.01 TIANGVVIEE611 pKa = 4.15 NARR614 pKa = 11.84 GGNGSDD620 pKa = 2.97 ALMGNSVANRR630 pKa = 11.84 LDD632 pKa = 3.58 GQNGDD637 pKa = 4.02 DD638 pKa = 3.8 GLLGRR643 pKa = 11.84 EE644 pKa = 4.4 GDD646 pKa = 3.98 DD647 pKa = 4.82 LLIGGNGKK655 pKa = 8.44 DD656 pKa = 3.62 TLNGGTGNDD665 pKa = 3.86 SLDD668 pKa = 3.56 GGTGRR673 pKa = 11.84 DD674 pKa = 3.77 VLNGGAGDD682 pKa = 3.78 DD683 pKa = 3.86 TLNGGLHH690 pKa = 7.34 DD691 pKa = 4.84 DD692 pKa = 4.11 LFVFEE697 pKa = 5.59 DD698 pKa = 3.86 AGTDD702 pKa = 3.69 TIVGYY707 pKa = 10.23 QKK709 pKa = 11.15 GEE711 pKa = 4.03 QFDD714 pKa = 4.56 LSALNVTWADD724 pKa = 3.36 VTIEE728 pKa = 4.0 SGRR731 pKa = 11.84 IGVDD735 pKa = 3.38 LEE737 pKa = 4.2 GDD739 pKa = 3.21 QDD741 pKa = 3.61 LTIIVNTSGITQGNFIFAA759 pKa = 4.52
Molecular weight: 80.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.897
IPC_protein 3.935
Toseland 3.706
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.617
Solomon 3.923
Lehninger 3.884
Nozaki 4.037
DTASelect 4.304
Thurlkill 3.757
EMBOSS 3.872
Sillero 4.062
Patrickios 0.82
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.922
Protein with the highest isoelectric point:
>tr|A0A328AU99|A0A328AU99_9CAUL Beta-ketoacyl-ACP reductase OS=Phenylobacterium deserti OX=1914756 GN=DJ018_08010 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.47 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTKK25 pKa = 10.02 NGQKK29 pKa = 9.43 ILARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.95 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3698
0
3698
1169876
27
1930
316.4
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.846 ± 0.065
0.722 ± 0.011
5.54 ± 0.028
5.902 ± 0.046
3.536 ± 0.025
8.937 ± 0.039
1.778 ± 0.02
4.071 ± 0.027
2.858 ± 0.03
10.116 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.148 ± 0.019
2.367 ± 0.03
5.655 ± 0.035
3.482 ± 0.024
7.729 ± 0.046
5.133 ± 0.033
5.061 ± 0.033
7.58 ± 0.034
1.438 ± 0.018
2.104 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here