Golovinomyces magnicellulatus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Golovinomyces

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A544ZUF2|A0A544ZUF2_9PEZI WD_REPEATS_REGION domain-containing protein (Fragment) OS=Golovinomyces magnicellulatus OX=62714 GN=Golomagni_06951 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 4.46GVMGTAPQGTMYY14 pKa = 10.34VRR16 pKa = 11.84ALYY19 pKa = 10.15DD20 pKa = 3.36YY21 pKa = 10.39EE22 pKa = 6.67ADD24 pKa = 4.05DD25 pKa = 3.81RR26 pKa = 11.84TSLSFHH32 pKa = 6.87EE33 pKa = 4.76GDD35 pKa = 3.79VIQVITQLEE44 pKa = 4.4SGWWDD49 pKa = 3.27GVINGVRR56 pKa = 11.84GWFPSNYY63 pKa = 8.13CQIVGGPDD71 pKa = 3.73DD72 pKa = 5.96LPDD75 pKa = 4.28HH76 pKa = 6.63LQQGQYY82 pKa = 11.26DD83 pKa = 4.36HH84 pKa = 7.39IDD86 pKa = 3.85DD87 pKa = 4.21EE88 pKa = 5.22NEE90 pKa = 3.91DD91 pKa = 3.6GEE93 pKa = 5.37AYY95 pKa = 10.32DD96 pKa = 3.72EE97 pKa = 4.3SFEE100 pKa = 4.72RR101 pKa = 11.84EE102 pKa = 4.13EE103 pKa = 4.76EE104 pKa = 4.13SDD106 pKa = 3.8DD107 pKa = 4.1DD108 pKa = 4.36APSRR112 pKa = 11.84LPLEE116 pKa = 4.6GTTAMGGGGGNSRR129 pKa = 11.84ADD131 pKa = 3.26FWIPQATPDD140 pKa = 4.26GRR142 pKa = 11.84LFYY145 pKa = 10.76YY146 pKa = 11.39NMMTGDD152 pKa = 3.84RR153 pKa = 11.84SMEE156 pKa = 4.15LPLEE160 pKa = 4.58SPTT163 pKa = 4.26

Molecular weight: 18.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A544ZYW2|A0A544ZYW2_9PEZI Calponin-homology (CH) domain-containing protein OS=Golovinomyces magnicellulatus OX=62714 GN=Golomagni_05385 PE=4 SV=1
RR1 pKa = 7.04RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84IEE61 pKa = 3.63ILIHH65 pKa = 6.24NSEE68 pKa = 4.39PTNISCVPTNTDD80 pKa = 2.31QWLKK84 pKa = 10.02GTTIDD89 pKa = 4.66SKK91 pKa = 11.53VV92 pKa = 2.94

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8170

0

8170

3553560

16

6734

435.0

48.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.976 ± 0.025

1.293 ± 0.011

5.563 ± 0.019

6.473 ± 0.028

3.905 ± 0.017

5.913 ± 0.028

2.293 ± 0.012

6.171 ± 0.023

6.112 ± 0.027

9.126 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.082 ± 0.01

4.748 ± 0.018

5.095 ± 0.022

4.049 ± 0.022

5.704 ± 0.022

9.016 ± 0.038

5.799 ± 0.016

5.595 ± 0.016

1.257 ± 0.01

2.818 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski