Mycobacterium phage Yecey3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649V8Z6|A0A649V8Z6_9CAUD Cas4 family exonuclease OS=Mycobacterium phage Yecey3 OX=2656617 GN=77 PE=4 SV=1
MM1 pKa = 7.35FGIPVATVDD10 pKa = 3.39QEE12 pKa = 4.54SYY14 pKa = 11.21EE15 pKa = 4.13EE16 pKa = 5.17EE17 pKa = 3.93EE18 pKa = 4.9LDD20 pKa = 2.97WSGYY24 pKa = 9.16IEE26 pKa = 5.64GGATHH31 pKa = 6.36NFEE34 pKa = 4.66RR35 pKa = 11.84ADD37 pKa = 3.68NNEE40 pKa = 3.84TSEE43 pKa = 4.21MEE45 pKa = 4.22GYY47 pKa = 10.24EE48 pKa = 4.1YY49 pKa = 11.32YY50 pKa = 10.66DD51 pKa = 4.84DD52 pKa = 4.59EE53 pKa = 5.13EE54 pKa = 6.14SDD56 pKa = 3.64FGFRR60 pKa = 11.84RR61 pKa = 11.84PP62 pKa = 3.5

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649V906|A0A649V906_9CAUD Uncharacterized protein OS=Mycobacterium phage Yecey3 OX=2656617 GN=87 PE=4 SV=1
MM1 pKa = 7.9PPRR4 pKa = 11.84ASIQQTADD12 pKa = 3.36YY13 pKa = 10.86LGVCTKK19 pKa = 8.01TVRR22 pKa = 11.84NYY24 pKa = 10.21IADD27 pKa = 3.64GRR29 pKa = 11.84LKK31 pKa = 10.45AVRR34 pKa = 11.84LGPRR38 pKa = 11.84LIRR41 pKa = 11.84VEE43 pKa = 4.05RR44 pKa = 11.84DD45 pKa = 3.15SVEE48 pKa = 3.78ALMRR52 pKa = 11.84PIGKK56 pKa = 9.51

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

16648

37

973

166.5

18.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.332 ± 0.402

0.895 ± 0.129

6.055 ± 0.226

6.746 ± 0.357

3.22 ± 0.182

8.343 ± 0.52

1.976 ± 0.171

5.028 ± 0.168

4.421 ± 0.2

8.181 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.535 ± 0.172

3.448 ± 0.231

5.316 ± 0.244

3.46 ± 0.234

6.553 ± 0.353

5.526 ± 0.191

6.055 ± 0.272

7.112 ± 0.227

1.988 ± 0.118

2.811 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski