uncultured phage_MedDCM-OCT-S38-C3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Stopalavirus; Stopalavirus S38C3

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4PGX3|A0A6S4PGX3_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S38-C3 OX=2740803 PE=4 SV=1
MM1 pKa = 7.49PRR3 pKa = 11.84QYY5 pKa = 11.43DD6 pKa = 3.87SNDD9 pKa = 3.18VNPFEE14 pKa = 5.81DD15 pKa = 4.38LGPLQFPPTDD25 pKa = 3.68EE26 pKa = 4.71NSPDD30 pKa = 4.23DD31 pKa = 3.89GSLIYY36 pKa = 10.47IHH38 pKa = 7.24GNTTYY43 pKa = 10.25IWDD46 pKa = 3.95GEE48 pKa = 4.21KK49 pKa = 8.34WTASTSGPRR58 pKa = 11.84LVDD61 pKa = 3.59LTWDD65 pKa = 3.93PNSGGGWLLNNAGADD80 pKa = 3.32VWFASVNEE88 pKa = 3.94VDD90 pKa = 4.48AGLMTPDD97 pKa = 2.68ILGRR101 pKa = 11.84IEE103 pKa = 4.48ALEE106 pKa = 4.02LHH108 pKa = 6.75GGAQGIVIKK117 pKa = 10.83GRR119 pKa = 11.84LDD121 pKa = 3.43SVGPPDD127 pKa = 4.1PSLAISVGDD136 pKa = 4.53AYY138 pKa = 11.44LDD140 pKa = 3.75TNGDD144 pKa = 3.24LWVCTEE150 pKa = 3.79EE151 pKa = 5.18LEE153 pKa = 4.35FVNVGMVQGPPGPQGPAGPQGPRR176 pKa = 11.84GYY178 pKa = 10.63DD179 pKa = 3.31GEE181 pKa = 4.68NGLDD185 pKa = 3.76GEE187 pKa = 4.71PGRR190 pKa = 11.84DD191 pKa = 3.65GTPGAQGPPGAPGRR205 pKa = 11.84DD206 pKa = 3.8GQPGAQGEE214 pKa = 4.12QGPQGEE220 pKa = 4.31KK221 pKa = 10.97GEE223 pKa = 4.49RR224 pKa = 11.84GWEE227 pKa = 4.28GATGPQGEE235 pKa = 4.7EE236 pKa = 4.79GPVGPQGPKK245 pKa = 10.04GDD247 pKa = 4.03RR248 pKa = 11.84GDD250 pKa = 3.79VGPEE254 pKa = 4.01GPVVGVEE261 pKa = 4.3GPEE264 pKa = 4.45GPVGPPGPEE273 pKa = 4.11GPEE276 pKa = 4.47GPDD279 pKa = 3.74GPQGPPGTSFANLKK293 pKa = 10.3GVVATEE299 pKa = 4.14HH300 pKa = 7.27DD301 pKa = 4.47LPADD305 pKa = 3.51PTQWDD310 pKa = 3.96MYY312 pKa = 10.59LVEE315 pKa = 4.98SADD318 pKa = 5.39KK319 pKa = 8.94YY320 pKa = 8.95TIWDD324 pKa = 3.77GEE326 pKa = 3.9KK327 pKa = 9.21WRR329 pKa = 11.84YY330 pKa = 7.89VAAGGSVEE338 pKa = 4.82DD339 pKa = 4.74APGDD343 pKa = 3.65SKK345 pKa = 11.5PYY347 pKa = 10.27VRR349 pKa = 11.84YY350 pKa = 10.7NMDD353 pKa = 2.47WRR355 pKa = 11.84EE356 pKa = 4.03GVLDD360 pKa = 4.72APDD363 pKa = 4.33DD364 pKa = 4.0GKK366 pKa = 11.65GYY368 pKa = 10.72VRR370 pKa = 11.84YY371 pKa = 8.28QQGWASLEE379 pKa = 4.21AVGLPYY385 pKa = 10.84LIGTDD390 pKa = 3.22KK391 pKa = 11.03TGLARR396 pKa = 11.84SVRR399 pKa = 11.84KK400 pKa = 9.89AGPFTNTKK408 pKa = 11.0DD409 pKa = 3.32MGPEE413 pKa = 3.45IEE415 pKa = 5.82LIDD418 pKa = 4.46GNGNYY423 pKa = 10.45SNVKK427 pKa = 9.15IGGSGGVVTSSDD439 pKa = 3.24AAGITVDD446 pKa = 4.86GSYY449 pKa = 11.12LLKK452 pKa = 10.7KK453 pKa = 10.57QFDD456 pKa = 4.35EE457 pKa = 4.71YY458 pKa = 10.24PTIDD462 pKa = 4.56AEE464 pKa = 4.53TLDD467 pKa = 4.3SVPADD472 pKa = 2.98SVMFAANQQGNKK484 pKa = 9.93SITWKK489 pKa = 10.8EE490 pKa = 3.7MMGSVMHH497 pKa = 6.24QIGFAIQSASITEE510 pKa = 4.32DD511 pKa = 3.22NAPAQIDD518 pKa = 3.63RR519 pKa = 11.84AVEE522 pKa = 4.36CTCQATLEE530 pKa = 4.27SSAPQFGEE538 pKa = 4.43DD539 pKa = 3.06VCTYY543 pKa = 8.88RR544 pKa = 11.84WRR546 pKa = 11.84AFPQGTSDD554 pKa = 3.32NAKK557 pKa = 10.29PEE559 pKa = 4.09WEE561 pKa = 4.08NGKK564 pKa = 9.66VPSNPNKK571 pKa = 10.05KK572 pKa = 10.41SFVFDD577 pKa = 3.29EE578 pKa = 4.82AGDD581 pKa = 3.77YY582 pKa = 10.57TIEE585 pKa = 4.54CEE587 pKa = 4.3VGASKK592 pKa = 10.89SKK594 pKa = 10.31LWNQVTKK601 pKa = 10.08VASYY605 pKa = 10.91DD606 pKa = 3.44FHH608 pKa = 8.3VKK610 pKa = 10.32EE611 pKa = 4.9GPDD614 pKa = 3.4PLMSYY619 pKa = 10.87AYY621 pKa = 10.32LHH623 pKa = 6.22VKK625 pKa = 10.26NITGGQIKK633 pKa = 9.8FPNALDD639 pKa = 4.1SGNHH643 pKa = 5.37YY644 pKa = 10.72YY645 pKa = 10.69DD646 pKa = 3.76WEE648 pKa = 4.46NMGHH652 pKa = 5.37EE653 pKa = 4.21PEE655 pKa = 4.88YY656 pKa = 10.36YY657 pKa = 10.77AFVHH661 pKa = 5.8VLGLEE666 pKa = 4.53GGDD669 pKa = 3.34QAFGGFKK676 pKa = 10.35CYY678 pKa = 10.56RR679 pKa = 11.84NVQGDD684 pKa = 3.35WDD686 pKa = 3.73DD687 pKa = 3.8WEE689 pKa = 4.46TDD691 pKa = 3.61KK692 pKa = 11.71SDD694 pKa = 3.68EE695 pKa = 4.24TCAKK699 pKa = 10.25YY700 pKa = 10.46GISFYY705 pKa = 11.52DD706 pKa = 3.18NDD708 pKa = 5.1LYY710 pKa = 11.47LEE712 pKa = 4.89PDD714 pKa = 3.0TGLEE718 pKa = 3.86YY719 pKa = 9.88MVYY722 pKa = 9.77MRR724 pKa = 11.84TQTYY728 pKa = 8.81NQVGGSDD735 pKa = 3.66DD736 pKa = 4.01SGVDD740 pKa = 3.54GLNQAGAWLDD750 pKa = 3.52GQRR753 pKa = 11.84WSTTGNATWEE763 pKa = 4.13WGEE766 pKa = 3.92LTDD769 pKa = 3.85TSKK772 pKa = 9.42VTSFYY777 pKa = 11.31RR778 pKa = 11.84MFEE781 pKa = 3.95QFSNSGSAWPTGWEE795 pKa = 4.12HH796 pKa = 7.06FDD798 pKa = 3.2TGKK801 pKa = 11.11GEE803 pKa = 3.8NFAWMFYY810 pKa = 7.6QARR813 pKa = 11.84ATGGIGCGMEE823 pKa = 3.86QWNMSKK829 pKa = 10.31ARR831 pKa = 11.84SLHH834 pKa = 5.48RR835 pKa = 11.84MFYY838 pKa = 9.38QAEE841 pKa = 4.14NFDD844 pKa = 4.53CDD846 pKa = 3.09ISNWDD851 pKa = 3.28TRR853 pKa = 11.84EE854 pKa = 3.84NEE856 pKa = 3.79TLYY859 pKa = 11.19YY860 pKa = 11.0FMGQCKK866 pKa = 10.02NFTCGGLNASEE877 pKa = 4.31EE878 pKa = 5.01GYY880 pKa = 8.66GTGIVNMKK888 pKa = 6.96TLKK891 pKa = 10.54VKK893 pKa = 10.19EE894 pKa = 4.2INYY897 pKa = 9.94SFYY900 pKa = 10.81QCEE903 pKa = 4.26KK904 pKa = 10.93LYY906 pKa = 10.31IDD908 pKa = 4.27CSQWGFPNLEE918 pKa = 4.0NFDD921 pKa = 4.27WDD923 pKa = 3.17TDD925 pKa = 3.73AAPDD929 pKa = 3.5VEE931 pKa = 4.86MFQTANIPRR940 pKa = 11.84SKK942 pKa = 11.01ASDD945 pKa = 3.26YY946 pKa = 10.92WGITSYY952 pKa = 11.52DD953 pKa = 3.84SYY955 pKa = 11.29WNVQIAGILPIWGTDD970 pKa = 3.24PSRR973 pKa = 11.84YY974 pKa = 8.21TSPQDD979 pKa = 3.42CPRR982 pKa = 11.84TGDD985 pKa = 3.27RR986 pKa = 11.84QKK988 pKa = 11.33AYY990 pKa = 9.56EE991 pKa = 4.12NTMATYY997 pKa = 10.48ADD999 pKa = 4.49EE1000 pKa = 4.39YY1001 pKa = 10.07AACGSSSSCKK1011 pKa = 10.39NEE1013 pKa = 4.1AKK1015 pKa = 10.45DD1016 pKa = 3.91KK1017 pKa = 10.73RR1018 pKa = 11.84DD1019 pKa = 3.43AKK1021 pKa = 10.71AVEE1024 pKa = 4.25DD1025 pKa = 4.09AKK1027 pKa = 11.52VPLL1030 pKa = 4.25

Molecular weight: 112.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PEE1|A0A6S4PEE1_9CAUD Uncharacterized protein OS=uncultured phage_MedDCM-OCT-S38-C3 OX=2740803 PE=4 SV=1
MM1 pKa = 7.65CFGGGGGGGTIVMPNTGAYY20 pKa = 10.96DD21 pKa = 3.39MMANAQISAMQSAMQMGTQRR41 pKa = 11.84VQAQLNDD48 pKa = 3.84SLRR51 pKa = 11.84MQGQYY56 pKa = 7.12QQRR59 pKa = 11.84LRR61 pKa = 11.84DD62 pKa = 3.68IQIEE66 pKa = 4.14RR67 pKa = 11.84AEE69 pKa = 4.12NTQAQARR76 pKa = 11.84RR77 pKa = 11.84MAEE80 pKa = 4.28LIGPPPPEE88 pKa = 4.13EE89 pKa = 3.79TAKK92 pKa = 11.14APGTNEE98 pKa = 3.43KK99 pKa = 9.8TSRR102 pKa = 11.84NNKK105 pKa = 8.8SRR107 pKa = 11.84LRR109 pKa = 11.84INRR112 pKa = 11.84PRR114 pKa = 11.84ATTSAQGTGLNINLGGG130 pKa = 3.61

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

13510

32

1124

300.2

33.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.511 ± 0.396

1.088 ± 0.189

6.38 ± 0.382

6.728 ± 0.236

3.124 ± 0.192

8.423 ± 0.413

1.288 ± 0.209

4.367 ± 0.235

5.278 ± 0.276

8.298 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.746 ± 0.195

4.308 ± 0.213

5.056 ± 0.345

5.3 ± 0.369

5.64 ± 0.466

5.759 ± 0.198

5.811 ± 0.387

6.092 ± 0.224

1.991 ± 0.241

2.813 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski