Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S38-C3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.363AlaAla: 10.363 ± 1.01
0.666AlaCys: 0.666 ± 0.177
5.996AlaAsp: 5.996 ± 0.586
7.254AlaGlu: 7.254 ± 1.038
3.405AlaPhe: 3.405 ± 0.486
9.031AlaGly: 9.031 ± 0.993
0.444AlaHis: 0.444 ± 0.162
3.405AlaIle: 3.405 ± 0.493
4.071AlaLys: 4.071 ± 0.417
8.365AlaLeu: 8.365 ± 1.099
2.887AlaMet: 2.887 ± 0.477
4.441AlaAsn: 4.441 ± 0.498
3.627AlaPro: 3.627 ± 0.651
5.774AlaGln: 5.774 ± 0.723
5.256AlaArg: 5.256 ± 0.785
5.182AlaSer: 5.182 ± 0.645
4.812AlaThr: 4.812 ± 0.61
5.33AlaVal: 5.33 ± 0.634
1.925AlaTrp: 1.925 ± 0.309
2.887AlaTyr: 2.887 ± 0.296
0.0AlaXaa: 0.0 ± 0.0
Cys
0.888CysAla: 0.888 ± 0.257
0.148CysCys: 0.148 ± 0.103
0.74CysAsp: 0.74 ± 0.24
0.592CysGlu: 0.592 ± 0.208
0.666CysPhe: 0.666 ± 0.277
1.258CysGly: 1.258 ± 0.346
0.222CysHis: 0.222 ± 0.121
0.666CysIle: 0.666 ± 0.292
0.518CysLys: 0.518 ± 0.187
0.666CysLeu: 0.666 ± 0.233
0.37CysMet: 0.37 ± 0.168
0.296CysAsn: 0.296 ± 0.161
0.444CysPro: 0.444 ± 0.186
0.222CysGln: 0.222 ± 0.126
0.74CysArg: 0.74 ± 0.313
0.814CysSer: 0.814 ± 0.235
0.74CysThr: 0.74 ± 0.257
0.518CysVal: 0.518 ± 0.213
0.074CysTrp: 0.074 ± 0.073
0.296CysTyr: 0.296 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
6.292AspAla: 6.292 ± 0.706
0.962AspCys: 0.962 ± 0.243
4.738AspAsp: 4.738 ± 0.902
4.441AspGlu: 4.441 ± 0.611
2.295AspPhe: 2.295 ± 0.341
6.44AspGly: 6.44 ± 1.303
0.444AspHis: 0.444 ± 0.239
2.369AspIle: 2.369 ± 0.377
3.183AspLys: 3.183 ± 0.603
6.662AspLeu: 6.662 ± 0.788
0.888AspMet: 0.888 ± 0.261
2.443AspAsn: 2.443 ± 0.311
4.367AspPro: 4.367 ± 0.623
2.591AspGln: 2.591 ± 0.437
3.479AspArg: 3.479 ± 0.422
3.331AspSer: 3.331 ± 0.618
2.739AspThr: 2.739 ± 0.59
3.109AspVal: 3.109 ± 0.44
1.629AspTrp: 1.629 ± 0.405
1.406AspTyr: 1.406 ± 0.199
0.0AspXaa: 0.0 ± 0.0
Glu
6.292GluAla: 6.292 ± 0.814
0.888GluCys: 0.888 ± 0.222
3.627GluAsp: 3.627 ± 0.482
4.812GluGlu: 4.812 ± 0.6
1.851GluPhe: 1.851 ± 0.285
6.144GluGly: 6.144 ± 0.558
1.036GluHis: 1.036 ± 0.272
3.849GluIle: 3.849 ± 0.581
4.071GluLys: 4.071 ± 0.585
7.551GluLeu: 7.551 ± 1.066
2.665GluMet: 2.665 ± 0.419
2.517GluAsn: 2.517 ± 0.574
2.887GluPro: 2.887 ± 0.486
2.813GluGln: 2.813 ± 0.421
3.257GluArg: 3.257 ± 0.577
2.665GluSer: 2.665 ± 0.407
3.109GluThr: 3.109 ± 0.356
3.923GluVal: 3.923 ± 0.489
1.629GluTrp: 1.629 ± 0.346
1.703GluTyr: 1.703 ± 0.404
0.0GluXaa: 0.0 ± 0.0
Phe
2.147PheAla: 2.147 ± 0.435
0.518PheCys: 0.518 ± 0.166
2.443PheAsp: 2.443 ± 0.346
1.777PheGlu: 1.777 ± 0.415
1.036PhePhe: 1.036 ± 0.331
2.221PheGly: 2.221 ± 0.435
0.814PheHis: 0.814 ± 0.281
0.888PheIle: 0.888 ± 0.216
1.851PheLys: 1.851 ± 0.41
2.665PheLeu: 2.665 ± 0.506
1.332PheMet: 1.332 ± 0.328
1.48PheAsn: 1.48 ± 0.351
1.258PhePro: 1.258 ± 0.301
1.48PheGln: 1.48 ± 0.321
1.629PheArg: 1.629 ± 0.381
2.591PheSer: 2.591 ± 0.41
1.925PheThr: 1.925 ± 0.259
1.555PheVal: 1.555 ± 0.3
0.444PheTrp: 0.444 ± 0.196
1.184PheTyr: 1.184 ± 0.374
0.0PheXaa: 0.0 ± 0.0
Gly
5.996GlyAla: 5.996 ± 0.579
0.592GlyCys: 0.592 ± 0.175
5.996GlyAsp: 5.996 ± 1.281
4.96GlyGlu: 4.96 ± 0.711
2.295GlyPhe: 2.295 ± 0.365
7.032GlyGly: 7.032 ± 1.084
1.332GlyHis: 1.332 ± 0.461
3.405GlyIle: 3.405 ± 0.499
4.738GlyLys: 4.738 ± 0.626
7.254GlyLeu: 7.254 ± 0.964
2.295GlyMet: 2.295 ± 0.483
4.219GlyAsn: 4.219 ± 0.485
5.404GlyPro: 5.404 ± 1.694
3.923GlyGln: 3.923 ± 0.559
4.886GlyArg: 4.886 ± 0.565
6.07GlySer: 6.07 ± 0.793
5.034GlyThr: 5.034 ± 0.788
5.034GlyVal: 5.034 ± 0.655
1.777GlyTrp: 1.777 ± 0.341
1.999GlyTyr: 1.999 ± 0.364
0.0GlyXaa: 0.0 ± 0.0
His
0.666HisAla: 0.666 ± 0.227
0.444HisCys: 0.444 ± 0.179
0.962HisAsp: 0.962 ± 0.303
0.888HisGlu: 0.888 ± 0.298
0.518HisPhe: 0.518 ± 0.179
0.888HisGly: 0.888 ± 0.268
0.296HisHis: 0.296 ± 0.171
0.592HisIle: 0.592 ± 0.259
0.37HisLys: 0.37 ± 0.163
1.11HisLeu: 1.11 ± 0.32
0.222HisMet: 0.222 ± 0.16
0.148HisAsn: 0.148 ± 0.106
0.592HisPro: 0.592 ± 0.248
1.11HisGln: 1.11 ± 0.361
0.962HisArg: 0.962 ± 0.274
0.666HisSer: 0.666 ± 0.234
0.592HisThr: 0.592 ± 0.208
0.814HisVal: 0.814 ± 0.28
0.444HisTrp: 0.444 ± 0.173
0.592HisTyr: 0.592 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
3.701IleAla: 3.701 ± 0.522
0.74IleCys: 0.74 ± 0.305
4.071IleAsp: 4.071 ± 0.559
3.849IleGlu: 3.849 ± 0.542
0.666IlePhe: 0.666 ± 0.217
4.071IleGly: 4.071 ± 0.535
0.592IleHis: 0.592 ± 0.192
1.036IleIle: 1.036 ± 0.225
2.591IleLys: 2.591 ± 0.343
2.369IleLeu: 2.369 ± 0.517
0.814IleMet: 0.814 ± 0.311
2.221IleAsn: 2.221 ± 0.34
1.703IlePro: 1.703 ± 0.414
2.295IleGln: 2.295 ± 0.356
2.443IleArg: 2.443 ± 0.422
2.887IleSer: 2.887 ± 0.375
3.849IleThr: 3.849 ± 0.664
1.703IleVal: 1.703 ± 0.304
0.962IleTrp: 0.962 ± 0.303
1.11IleTyr: 1.11 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
5.922LysAla: 5.922 ± 0.596
0.37LysCys: 0.37 ± 0.152
3.405LysAsp: 3.405 ± 0.695
3.405LysGlu: 3.405 ± 0.38
1.925LysPhe: 1.925 ± 0.354
4.071LysGly: 4.071 ± 0.629
0.37LysHis: 0.37 ± 0.196
2.591LysIle: 2.591 ± 0.37
3.035LysLys: 3.035 ± 0.556
3.849LysLeu: 3.849 ± 0.655
0.962LysMet: 0.962 ± 0.3
2.295LysAsn: 2.295 ± 0.383
3.479LysPro: 3.479 ± 0.514
2.665LysGln: 2.665 ± 0.481
2.517LysArg: 2.517 ± 0.504
2.887LysSer: 2.887 ± 0.425
2.591LysThr: 2.591 ± 0.412
4.145LysVal: 4.145 ± 0.41
1.11LysTrp: 1.11 ± 0.377
1.11LysTyr: 1.11 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
7.847LeuAla: 7.847 ± 0.954
0.888LeuCys: 0.888 ± 0.244
4.664LeuAsp: 4.664 ± 0.585
6.07LeuGlu: 6.07 ± 0.797
1.703LeuPhe: 1.703 ± 0.367
6.44LeuGly: 6.44 ± 0.863
1.184LeuHis: 1.184 ± 0.342
4.219LeuIle: 4.219 ± 0.717
5.33LeuLys: 5.33 ± 0.736
6.366LeuLeu: 6.366 ± 0.816
2.887LeuMet: 2.887 ± 0.549
3.997LeuAsn: 3.997 ± 0.732
4.738LeuPro: 4.738 ± 0.593
3.997LeuGln: 3.997 ± 0.68
5.182LeuArg: 5.182 ± 0.767
4.367LeuSer: 4.367 ± 0.604
5.7LeuThr: 5.7 ± 0.813
4.441LeuVal: 4.441 ± 0.592
1.258LeuTrp: 1.258 ± 0.287
1.703LeuTyr: 1.703 ± 0.299
0.0LeuXaa: 0.0 ± 0.0
Met
3.479MetAla: 3.479 ± 0.494
0.518MetCys: 0.518 ± 0.17
1.629MetAsp: 1.629 ± 0.366
1.332MetGlu: 1.332 ± 0.389
0.814MetPhe: 0.814 ± 0.339
2.147MetGly: 2.147 ± 0.55
0.296MetHis: 0.296 ± 0.139
1.406MetIle: 1.406 ± 0.273
0.814MetLys: 0.814 ± 0.231
2.073MetLeu: 2.073 ± 0.335
0.37MetMet: 0.37 ± 0.157
1.036MetAsn: 1.036 ± 0.312
1.703MetPro: 1.703 ± 0.384
1.406MetGln: 1.406 ± 0.481
2.221MetArg: 2.221 ± 0.362
2.443MetSer: 2.443 ± 0.451
1.555MetThr: 1.555 ± 0.303
1.555MetVal: 1.555 ± 0.326
0.148MetTrp: 0.148 ± 0.104
0.518MetTyr: 0.518 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
4.516AsnAla: 4.516 ± 0.75
0.222AsnCys: 0.222 ± 0.122
2.517AsnAsp: 2.517 ± 0.587
1.925AsnGlu: 1.925 ± 0.439
1.48AsnPhe: 1.48 ± 0.299
3.701AsnGly: 3.701 ± 0.477
0.74AsnHis: 0.74 ± 0.207
2.369AsnIle: 2.369 ± 0.436
2.073AsnLys: 2.073 ± 0.529
3.257AsnLeu: 3.257 ± 0.559
1.332AsnMet: 1.332 ± 0.294
1.851AsnAsn: 1.851 ± 0.422
2.665AsnPro: 2.665 ± 0.609
1.925AsnGln: 1.925 ± 0.39
2.665AsnArg: 2.665 ± 0.493
2.147AsnSer: 2.147 ± 0.36
2.739AsnThr: 2.739 ± 0.512
2.887AsnVal: 2.887 ± 0.591
0.37AsnTrp: 0.37 ± 0.175
1.703AsnTyr: 1.703 ± 0.372
0.0AsnXaa: 0.0 ± 0.0
Pro
5.182ProAla: 5.182 ± 0.727
0.37ProCys: 0.37 ± 0.152
3.849ProAsp: 3.849 ± 0.633
4.59ProGlu: 4.59 ± 0.646
1.332ProPhe: 1.332 ± 0.301
3.923ProGly: 3.923 ± 0.666
0.888ProHis: 0.888 ± 0.315
1.629ProIle: 1.629 ± 0.348
2.813ProLys: 2.813 ± 0.43
3.627ProLeu: 3.627 ± 0.393
0.814ProMet: 0.814 ± 0.245
1.851ProAsn: 1.851 ± 0.444
3.035ProPro: 3.035 ± 0.676
1.999ProGln: 1.999 ± 0.607
1.851ProArg: 1.851 ± 0.479
3.405ProSer: 3.405 ± 0.387
3.183ProThr: 3.183 ± 0.49
3.331ProVal: 3.331 ± 0.474
1.184ProTrp: 1.184 ± 0.455
1.703ProTyr: 1.703 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
5.552GlnAla: 5.552 ± 0.892
0.666GlnCys: 0.666 ± 0.207
1.555GlnAsp: 1.555 ± 0.261
3.701GlnGlu: 3.701 ± 0.818
2.443GlnPhe: 2.443 ± 0.384
4.441GlnGly: 4.441 ± 0.918
0.814GlnHis: 0.814 ± 0.283
2.369GlnIle: 2.369 ± 0.448
1.851GlnLys: 1.851 ± 0.375
3.849GlnLeu: 3.849 ± 0.669
1.999GlnMet: 1.999 ± 0.41
1.332GlnAsn: 1.332 ± 0.343
2.073GlnPro: 2.073 ± 0.373
5.33GlnGln: 5.33 ± 1.122
3.257GlnArg: 3.257 ± 0.533
2.443GlnSer: 2.443 ± 0.352
2.591GlnThr: 2.591 ± 0.364
3.923GlnVal: 3.923 ± 0.51
1.036GlnTrp: 1.036 ± 0.303
1.777GlnTyr: 1.777 ± 0.617
0.0GlnXaa: 0.0 ± 0.0
Arg
5.626ArgAla: 5.626 ± 0.725
0.518ArgCys: 0.518 ± 0.209
2.739ArgAsp: 2.739 ± 0.429
3.553ArgGlu: 3.553 ± 0.714
1.777ArgPhe: 1.777 ± 0.37
4.071ArgGly: 4.071 ± 0.644
0.74ArgHis: 0.74 ± 0.328
2.739ArgIle: 2.739 ± 0.511
3.923ArgLys: 3.923 ± 0.502
5.182ArgLeu: 5.182 ± 0.753
1.48ArgMet: 1.48 ± 0.293
2.147ArgAsn: 2.147 ± 0.38
2.443ArgPro: 2.443 ± 0.453
3.849ArgGln: 3.849 ± 0.732
3.035ArgArg: 3.035 ± 0.625
2.443ArgSer: 2.443 ± 0.321
2.739ArgThr: 2.739 ± 0.457
3.849ArgVal: 3.849 ± 0.576
1.555ArgTrp: 1.555 ± 0.381
1.999ArgTyr: 1.999 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
5.33SerAla: 5.33 ± 0.653
0.592SerCys: 0.592 ± 0.195
4.071SerAsp: 4.071 ± 0.628
3.035SerGlu: 3.035 ± 0.499
1.777SerPhe: 1.777 ± 0.488
5.478SerGly: 5.478 ± 0.823
0.592SerHis: 0.592 ± 0.238
1.851SerIle: 1.851 ± 0.394
2.961SerLys: 2.961 ± 0.515
3.923SerLeu: 3.923 ± 0.615
1.258SerMet: 1.258 ± 0.28
2.517SerAsn: 2.517 ± 0.348
3.035SerPro: 3.035 ± 0.361
2.739SerGln: 2.739 ± 0.415
3.183SerArg: 3.183 ± 0.575
4.071SerSer: 4.071 ± 0.6
3.701SerThr: 3.701 ± 0.635
4.145SerVal: 4.145 ± 0.516
1.406SerTrp: 1.406 ± 0.37
1.925SerTyr: 1.925 ± 0.364
0.0SerXaa: 0.0 ± 0.0
Thr
5.848ThrAla: 5.848 ± 0.847
0.444ThrCys: 0.444 ± 0.225
3.701ThrAsp: 3.701 ± 0.629
3.701ThrGlu: 3.701 ± 0.337
1.999ThrPhe: 1.999 ± 0.423
5.774ThrGly: 5.774 ± 0.684
0.37ThrHis: 0.37 ± 0.144
3.553ThrIle: 3.553 ± 0.781
2.887ThrLys: 2.887 ± 0.312
4.367ThrLeu: 4.367 ± 0.73
0.962ThrMet: 0.962 ± 0.255
2.887ThrAsn: 2.887 ± 0.723
2.665ThrPro: 2.665 ± 0.393
2.591ThrGln: 2.591 ± 0.478
2.813ThrArg: 2.813 ± 0.382
3.479ThrSer: 3.479 ± 0.512
3.553ThrThr: 3.553 ± 0.554
3.923ThrVal: 3.923 ± 0.81
1.332ThrTrp: 1.332 ± 0.224
1.258ThrTyr: 1.258 ± 0.309
0.0ThrXaa: 0.0 ± 0.0
Val
5.552ValAla: 5.552 ± 0.633
0.74ValCys: 0.74 ± 0.295
3.627ValAsp: 3.627 ± 0.548
4.59ValGlu: 4.59 ± 0.585
1.999ValPhe: 1.999 ± 0.377
4.219ValGly: 4.219 ± 0.648
0.74ValHis: 0.74 ± 0.264
2.739ValIle: 2.739 ± 0.57
3.109ValLys: 3.109 ± 0.425
5.182ValLeu: 5.182 ± 0.594
2.073ValMet: 2.073 ± 0.501
3.035ValAsn: 3.035 ± 0.379
2.517ValPro: 2.517 ± 0.34
3.183ValGln: 3.183 ± 0.679
3.997ValArg: 3.997 ± 0.527
3.109ValSer: 3.109 ± 0.439
3.775ValThr: 3.775 ± 0.786
3.849ValVal: 3.849 ± 0.521
0.666ValTrp: 0.666 ± 0.235
2.221ValTyr: 2.221 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
1.406TrpAla: 1.406 ± 0.42
0.296TrpCys: 0.296 ± 0.194
1.851TrpAsp: 1.851 ± 0.507
1.851TrpGlu: 1.851 ± 0.466
0.444TrpPhe: 0.444 ± 0.128
1.036TrpGly: 1.036 ± 0.317
0.296TrpHis: 0.296 ± 0.154
0.74TrpIle: 0.74 ± 0.253
0.962TrpLys: 0.962 ± 0.269
1.851TrpLeu: 1.851 ± 0.442
0.74TrpMet: 0.74 ± 0.223
1.258TrpAsn: 1.258 ± 0.345
0.814TrpPro: 0.814 ± 0.217
1.11TrpGln: 1.11 ± 0.436
1.555TrpArg: 1.555 ± 0.303
1.184TrpSer: 1.184 ± 0.416
1.11TrpThr: 1.11 ± 0.217
0.962TrpVal: 0.962 ± 0.325
0.222TrpTrp: 0.222 ± 0.143
0.222TrpTyr: 0.222 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.517TyrAla: 2.517 ± 0.419
0.296TyrCys: 0.296 ± 0.154
1.629TyrAsp: 1.629 ± 0.494
0.962TyrGlu: 0.962 ± 0.242
0.814TyrPhe: 0.814 ± 0.319
1.851TyrGly: 1.851 ± 0.379
0.666TyrHis: 0.666 ± 0.209
1.258TyrIle: 1.258 ± 0.319
1.629TyrLys: 1.629 ± 0.326
2.813TyrLeu: 2.813 ± 0.434
1.036TyrMet: 1.036 ± 0.258
1.11TyrAsn: 1.11 ± 0.342
1.11TyrPro: 1.11 ± 0.283
1.999TyrGln: 1.999 ± 0.373
1.48TyrArg: 1.48 ± 0.303
1.406TyrSer: 1.406 ± 0.263
2.073TyrThr: 2.073 ± 0.338
1.925TyrVal: 1.925 ± 0.324
0.74TyrTrp: 0.74 ± 0.214
0.814TyrTyr: 0.814 ± 0.278
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (13510 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski