Ralstonia phage Alix

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Gervaisevirus; unclassified Gervaisevirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5B888|A0A7G5B888_9CAUD Uncharacterized protein OS=Ralstonia phage Alix OX=2759718 GN=20A_00062 PE=4 SV=1
MM1 pKa = 6.29TTKK4 pKa = 10.56HH5 pKa = 6.52SDD7 pKa = 3.34EE8 pKa = 4.94LLDD11 pKa = 3.87GLTPEE16 pKa = 4.77EE17 pKa = 4.09LAALNDD23 pKa = 4.22DD24 pKa = 4.71DD25 pKa = 5.74GGAAGGEE32 pKa = 4.13DD33 pKa = 3.89GGAGGDD39 pKa = 3.79DD40 pKa = 3.78GAGEE44 pKa = 4.44GTGEE48 pKa = 4.17GAGAKK53 pKa = 9.59NAGGEE58 pKa = 4.2GDD60 pKa = 4.32DD61 pKa = 4.66GANAGAGAGADD72 pKa = 3.37AGAGGEE78 pKa = 4.47DD79 pKa = 3.66GGAGAGEE86 pKa = 4.45GGGEE90 pKa = 4.16GDD92 pKa = 4.25GGAVDD97 pKa = 4.13TGSKK101 pKa = 10.52SMPVYY106 pKa = 9.87VAEE109 pKa = 4.31VPEE112 pKa = 4.67DD113 pKa = 3.63ADD115 pKa = 4.01AKK117 pKa = 11.05LKK119 pKa = 11.01GLADD123 pKa = 3.32KK124 pKa = 10.92RR125 pKa = 11.84ADD127 pKa = 4.02LRR129 pKa = 11.84KK130 pKa = 10.09QYY132 pKa = 11.27DD133 pKa = 3.6DD134 pKa = 4.16GDD136 pKa = 3.68ITFDD140 pKa = 3.55EE141 pKa = 4.94LEE143 pKa = 4.16SARR146 pKa = 11.84DD147 pKa = 3.67EE148 pKa = 4.05ITKK151 pKa = 9.95EE152 pKa = 3.63EE153 pKa = 4.17KK154 pKa = 10.46AIEE157 pKa = 4.04RR158 pKa = 11.84AQLKK162 pKa = 10.89AEE164 pKa = 4.45MAAEE168 pKa = 4.1MQQQQTINDD177 pKa = 3.71WNRR180 pKa = 11.84DD181 pKa = 3.5VNSFLDD187 pKa = 3.59ANPEE191 pKa = 3.82YY192 pKa = 10.19RR193 pKa = 11.84ASDD196 pKa = 3.42VRR198 pKa = 11.84YY199 pKa = 8.9QALDD203 pKa = 3.13MMVRR207 pKa = 11.84KK208 pKa = 10.04IGGAPEE214 pKa = 4.07AAKK217 pKa = 8.36MTGPQVLAKK226 pKa = 10.47AHH228 pKa = 6.63EE229 pKa = 4.31EE230 pKa = 3.91LQKK233 pKa = 11.23AFGVAAPAKK242 pKa = 9.75KK243 pKa = 10.38DD244 pKa = 3.29EE245 pKa = 4.56GNGKK249 pKa = 9.33GEE251 pKa = 4.32KK252 pKa = 9.93KK253 pKa = 10.06PPPKK257 pKa = 10.3VDD259 pKa = 3.26VPPTLGKK266 pKa = 10.62LPAAEE271 pKa = 4.49NNDD274 pKa = 3.83TQGGKK279 pKa = 9.61YY280 pKa = 9.66AALDD284 pKa = 3.62RR285 pKa = 11.84LLEE288 pKa = 4.24TDD290 pKa = 3.99PLAHH294 pKa = 6.99EE295 pKa = 4.87DD296 pKa = 3.8ALAKK300 pKa = 9.99MSAGEE305 pKa = 3.83RR306 pKa = 11.84DD307 pKa = 3.52AYY309 pKa = 10.69LAARR313 pKa = 4.41

Molecular weight: 31.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5B853|A0A7G5B853_9CAUD Uncharacterized protein OS=Ralstonia phage Alix OX=2759718 GN=20A_00027 PE=4 SV=1
MM1 pKa = 7.6PCSEE5 pKa = 3.6QRR7 pKa = 11.84ARR9 pKa = 11.84KK10 pKa = 9.03LLSAGRR16 pKa = 11.84ARR18 pKa = 11.84VHH20 pKa = 6.32RR21 pKa = 11.84LYY23 pKa = 10.32PFTIRR28 pKa = 11.84LIDD31 pKa = 3.61RR32 pKa = 11.84QQANSVLQPLEE43 pKa = 4.54LKK45 pKa = 10.42LDD47 pKa = 4.35PGSKK51 pKa = 6.87TTGLAVCRR59 pKa = 11.84VEE61 pKa = 4.85EE62 pKa = 4.68RR63 pKa = 11.84IDD65 pKa = 3.29VDD67 pKa = 3.97GVVEE71 pKa = 4.1RR72 pKa = 11.84VMHH75 pKa = 6.39IRR77 pKa = 11.84FLMDD81 pKa = 3.92LVHH84 pKa = 6.97RR85 pKa = 11.84GHH87 pKa = 7.53AIKK90 pKa = 10.34EE91 pKa = 4.13ALHH94 pKa = 6.32ARR96 pKa = 11.84AAMRR100 pKa = 11.84RR101 pKa = 11.84RR102 pKa = 11.84RR103 pKa = 11.84RR104 pKa = 11.84GNLRR108 pKa = 11.84YY109 pKa = 9.46RR110 pKa = 11.84APRR113 pKa = 11.84FDD115 pKa = 2.98NRR117 pKa = 11.84TRR119 pKa = 11.84KK120 pKa = 9.91EE121 pKa = 3.62GWLPPSLQHH130 pKa = 7.23RR131 pKa = 11.84VDD133 pKa = 3.81TTVSWVKK140 pKa = 10.15RR141 pKa = 11.84LCRR144 pKa = 11.84LAPITHH150 pKa = 7.53LAQEE154 pKa = 4.45LVRR157 pKa = 11.84FDD159 pKa = 3.56MQLMQNAEE167 pKa = 3.87ISGVEE172 pKa = 4.02YY173 pKa = 10.62QQGEE177 pKa = 4.04LAGYY181 pKa = 7.23EE182 pKa = 3.88VRR184 pKa = 11.84EE185 pKa = 3.92YY186 pKa = 11.21LLEE189 pKa = 4.35KK190 pKa = 10.41FGRR193 pKa = 11.84TCQYY197 pKa = 10.63CDD199 pKa = 3.25EE200 pKa = 4.8TGVPLQVEE208 pKa = 4.92HH209 pKa = 6.68IHH211 pKa = 6.08PKK213 pKa = 10.4ARR215 pKa = 11.84GGSNRR220 pKa = 11.84VSNLTLACQPCNEE233 pKa = 4.9KK234 pKa = 10.41KK235 pKa = 10.31DD236 pKa = 3.8SQDD239 pKa = 2.61IRR241 pKa = 11.84VFLAKK246 pKa = 10.57DD247 pKa = 3.18PMRR250 pKa = 11.84LEE252 pKa = 5.13RR253 pKa = 11.84ILARR257 pKa = 11.84AKK259 pKa = 10.84APLRR263 pKa = 11.84DD264 pKa = 3.54AAAVNATRR272 pKa = 11.84WALLNALKK280 pKa = 9.45GTGLPVNTGSGGQTKK295 pKa = 9.43WNRR298 pKa = 11.84TRR300 pKa = 11.84LGLPKK305 pKa = 9.65THH307 pKa = 6.85SLDD310 pKa = 3.5AACVGRR316 pKa = 11.84ADD318 pKa = 3.88RR319 pKa = 11.84VTGSGAPVLVVKK331 pKa = 9.21CTGRR335 pKa = 11.84GSRR338 pKa = 11.84SRR340 pKa = 11.84TRR342 pKa = 11.84LNQYY346 pKa = 8.61GFPRR350 pKa = 11.84AYY352 pKa = 10.63LARR355 pKa = 11.84NKK357 pKa = 9.22TAFGFRR363 pKa = 11.84TGDD366 pKa = 3.32MVIASVPSGKK376 pKa = 10.13KK377 pKa = 9.39AGTYY381 pKa = 9.16KK382 pKa = 10.63GRR384 pKa = 11.84VAIRR388 pKa = 11.84QTGSFNIQPGKK399 pKa = 9.8PGLPTVQGISHH410 pKa = 6.39KK411 pKa = 9.73HH412 pKa = 5.4CSVAQRR418 pKa = 11.84GDD420 pKa = 3.17GYY422 pKa = 11.29GYY424 pKa = 11.51ALASPVNSQPGKK436 pKa = 8.23ATRR439 pKa = 11.84PAASALYY446 pKa = 10.63LLGLKK451 pKa = 10.52AEE453 pKa = 4.39VSRR456 pKa = 11.84ADD458 pKa = 3.14

Molecular weight: 50.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

17536

32

3164

250.5

27.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.698 ± 0.609

0.861 ± 0.16

6.028 ± 0.186

6.233 ± 0.368

2.891 ± 0.159

8.639 ± 0.388

1.979 ± 0.166

4.34 ± 0.27

4.653 ± 0.325

7.031 ± 0.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.155

3.627 ± 0.224

4.995 ± 0.231

4.06 ± 0.228

7.003 ± 0.331

5.628 ± 0.238

5.514 ± 0.261

6.301 ± 0.177

1.391 ± 0.165

2.492 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski