Amino acid dipepetide frequency for Ralstonia phage Alix

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.04AlaAla: 23.04 ± 1.734
1.369AlaCys: 1.369 ± 0.471
5.931AlaAsp: 5.931 ± 0.551
9.524AlaGlu: 9.524 ± 1.308
3.707AlaPhe: 3.707 ± 0.464
11.691AlaGly: 11.691 ± 1.327
2.452AlaHis: 2.452 ± 0.355
5.304AlaIle: 5.304 ± 0.601
6.216AlaLys: 6.216 ± 0.709
10.037AlaLeu: 10.037 ± 0.671
4.106AlaMet: 4.106 ± 0.513
4.448AlaAsn: 4.448 ± 0.691
8.155AlaPro: 8.155 ± 1.34
5.988AlaGln: 5.988 ± 0.508
8.497AlaArg: 8.497 ± 0.707
8.041AlaSer: 8.041 ± 0.803
5.532AlaThr: 5.532 ± 0.512
7.699AlaVal: 7.699 ± 0.541
1.825AlaTrp: 1.825 ± 0.319
3.422AlaTyr: 3.422 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.741CysAla: 0.741 ± 0.228
0.228CysCys: 0.228 ± 0.124
0.513CysAsp: 0.513 ± 0.209
0.285CysGlu: 0.285 ± 0.167
0.285CysPhe: 0.285 ± 0.159
0.855CysGly: 0.855 ± 0.307
0.285CysHis: 0.285 ± 0.175
0.342CysIle: 0.342 ± 0.146
0.627CysLys: 0.627 ± 0.218
0.627CysLeu: 0.627 ± 0.257
0.171CysMet: 0.171 ± 0.109
0.285CysAsn: 0.285 ± 0.14
0.342CysPro: 0.342 ± 0.187
0.114CysGln: 0.114 ± 0.132
0.684CysArg: 0.684 ± 0.249
0.627CysSer: 0.627 ± 0.234
0.456CysThr: 0.456 ± 0.203
0.627CysVal: 0.627 ± 0.226
0.228CysTrp: 0.228 ± 0.111
0.285CysTyr: 0.285 ± 0.214
0.0CysXaa: 0.0 ± 0.0
Asp
8.725AspAla: 8.725 ± 0.727
0.399AspCys: 0.399 ± 0.227
3.878AspAsp: 3.878 ± 0.653
3.992AspGlu: 3.992 ± 0.527
1.654AspPhe: 1.654 ± 0.243
7.414AspGly: 7.414 ± 0.697
1.141AspHis: 1.141 ± 0.267
2.908AspIle: 2.908 ± 0.412
1.939AspLys: 1.939 ± 0.386
4.334AspLeu: 4.334 ± 0.474
1.312AspMet: 1.312 ± 0.339
2.167AspAsn: 2.167 ± 0.323
2.623AspPro: 2.623 ± 0.364
1.312AspGln: 1.312 ± 0.245
3.992AspArg: 3.992 ± 0.461
2.509AspSer: 2.509 ± 0.429
3.023AspThr: 3.023 ± 0.374
4.277AspVal: 4.277 ± 0.547
0.969AspTrp: 0.969 ± 0.222
1.711AspTyr: 1.711 ± 0.279
0.0AspXaa: 0.0 ± 0.0
Glu
8.383GluAla: 8.383 ± 1.143
0.399GluCys: 0.399 ± 0.182
3.479GluAsp: 3.479 ± 0.473
3.992GluGlu: 3.992 ± 0.565
1.939GluPhe: 1.939 ± 0.302
4.448GluGly: 4.448 ± 0.772
1.483GluHis: 1.483 ± 0.332
3.08GluIle: 3.08 ± 0.319
2.509GluLys: 2.509 ± 0.412
5.532GluLeu: 5.532 ± 0.494
1.882GluMet: 1.882 ± 0.372
2.395GluAsn: 2.395 ± 0.388
3.365GluPro: 3.365 ± 0.599
3.08GluGln: 3.08 ± 0.516
6.102GluArg: 6.102 ± 0.64
2.908GluSer: 2.908 ± 0.482
2.452GluThr: 2.452 ± 0.401
2.509GluVal: 2.509 ± 0.402
1.027GluTrp: 1.027 ± 0.228
1.369GluTyr: 1.369 ± 0.247
0.0GluXaa: 0.0 ± 0.0
Phe
3.023PheAla: 3.023 ± 0.388
0.399PheCys: 0.399 ± 0.151
2.68PheAsp: 2.68 ± 0.408
1.825PheGlu: 1.825 ± 0.329
0.57PhePhe: 0.57 ± 0.216
2.623PheGly: 2.623 ± 0.358
0.456PheHis: 0.456 ± 0.133
1.426PheIle: 1.426 ± 0.324
1.027PheLys: 1.027 ± 0.261
1.768PheLeu: 1.768 ± 0.384
0.912PheMet: 0.912 ± 0.281
1.084PheAsn: 1.084 ± 0.21
1.084PhePro: 1.084 ± 0.267
0.855PheGln: 0.855 ± 0.238
1.54PheArg: 1.54 ± 0.468
1.54PheSer: 1.54 ± 0.261
1.369PheThr: 1.369 ± 0.27
3.023PheVal: 3.023 ± 0.438
0.513PheTrp: 0.513 ± 0.178
1.198PheTyr: 1.198 ± 0.218
0.0PheXaa: 0.0 ± 0.0
Gly
11.634GlyAla: 11.634 ± 1.527
0.741GlyCys: 0.741 ± 0.302
6.159GlyAsp: 6.159 ± 0.537
5.475GlyGlu: 5.475 ± 0.723
2.224GlyPhe: 2.224 ± 0.342
8.155GlyGly: 8.155 ± 1.205
1.654GlyHis: 1.654 ± 0.359
3.365GlyIle: 3.365 ± 0.434
4.562GlyLys: 4.562 ± 0.522
5.475GlyLeu: 5.475 ± 0.66
1.996GlyMet: 1.996 ± 0.335
3.251GlyAsn: 3.251 ± 0.578
2.281GlyPro: 2.281 ± 0.337
3.308GlyGln: 3.308 ± 0.391
5.988GlyArg: 5.988 ± 0.706
4.79GlySer: 4.79 ± 0.599
6.045GlyThr: 6.045 ± 0.779
5.532GlyVal: 5.532 ± 0.54
1.255GlyTrp: 1.255 ± 0.213
2.509GlyTyr: 2.509 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
2.167HisAla: 2.167 ± 0.303
0.513HisCys: 0.513 ± 0.255
1.483HisAsp: 1.483 ± 0.272
1.654HisGlu: 1.654 ± 0.294
0.912HisPhe: 0.912 ± 0.204
1.54HisGly: 1.54 ± 0.349
0.684HisHis: 0.684 ± 0.187
1.084HisIle: 1.084 ± 0.249
0.57HisLys: 0.57 ± 0.204
1.312HisLeu: 1.312 ± 0.248
0.456HisMet: 0.456 ± 0.165
0.798HisAsn: 0.798 ± 0.238
0.855HisPro: 0.855 ± 0.208
0.57HisGln: 0.57 ± 0.147
1.597HisArg: 1.597 ± 0.304
1.426HisSer: 1.426 ± 0.317
0.798HisThr: 0.798 ± 0.182
0.855HisVal: 0.855 ± 0.238
0.114HisTrp: 0.114 ± 0.084
0.399HisTyr: 0.399 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
5.76IleAla: 5.76 ± 0.604
0.171IleCys: 0.171 ± 0.108
2.68IleAsp: 2.68 ± 0.394
4.22IleGlu: 4.22 ± 0.603
0.969IlePhe: 0.969 ± 0.25
4.163IleGly: 4.163 ± 0.573
0.57IleHis: 0.57 ± 0.15
1.483IleIle: 1.483 ± 0.304
1.996IleLys: 1.996 ± 0.356
2.053IleLeu: 2.053 ± 0.282
1.141IleMet: 1.141 ± 0.304
2.053IleAsn: 2.053 ± 0.334
1.654IlePro: 1.654 ± 0.318
1.768IleGln: 1.768 ± 0.38
3.08IleArg: 3.08 ± 0.548
2.452IleSer: 2.452 ± 0.422
2.68IleThr: 2.68 ± 0.524
3.137IleVal: 3.137 ± 0.513
0.285IleTrp: 0.285 ± 0.123
1.084IleTyr: 1.084 ± 0.238
0.0IleXaa: 0.0 ± 0.0
Lys
6.729LysAla: 6.729 ± 0.588
0.399LysCys: 0.399 ± 0.156
2.566LysAsp: 2.566 ± 0.421
2.338LysGlu: 2.338 ± 0.441
1.312LysPhe: 1.312 ± 0.307
3.194LysGly: 3.194 ± 0.501
0.627LysHis: 0.627 ± 0.19
1.768LysIle: 1.768 ± 0.368
2.053LysLys: 2.053 ± 0.347
3.707LysLeu: 3.707 ± 0.404
1.312LysMet: 1.312 ± 0.293
1.882LysAsn: 1.882 ± 0.368
3.023LysPro: 3.023 ± 0.402
2.281LysGln: 2.281 ± 0.382
3.992LysArg: 3.992 ± 0.54
2.053LysSer: 2.053 ± 0.508
2.68LysThr: 2.68 ± 0.443
2.851LysVal: 2.851 ± 0.419
0.969LysTrp: 0.969 ± 0.251
0.798LysTyr: 0.798 ± 0.156
0.0LysXaa: 0.0 ± 0.0
Leu
8.098LeuAla: 8.098 ± 0.696
0.456LeuCys: 0.456 ± 0.172
5.589LeuAsp: 5.589 ± 0.501
3.593LeuGlu: 3.593 ± 0.419
2.395LeuPhe: 2.395 ± 0.434
5.475LeuGly: 5.475 ± 0.581
1.369LeuHis: 1.369 ± 0.275
3.422LeuIle: 3.422 ± 0.587
4.391LeuLys: 4.391 ± 0.672
4.505LeuLeu: 4.505 ± 0.531
1.483LeuMet: 1.483 ± 0.279
2.167LeuAsn: 2.167 ± 0.308
4.22LeuPro: 4.22 ± 0.818
2.338LeuGln: 2.338 ± 0.273
5.247LeuArg: 5.247 ± 0.441
4.676LeuSer: 4.676 ± 0.508
4.847LeuThr: 4.847 ± 0.48
3.707LeuVal: 3.707 ± 0.607
0.798LeuTrp: 0.798 ± 0.232
1.54LeuTyr: 1.54 ± 0.305
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.418
0.114MetCys: 0.114 ± 0.09
1.369MetAsp: 1.369 ± 0.313
1.141MetGlu: 1.141 ± 0.271
0.912MetPhe: 0.912 ± 0.272
2.11MetGly: 2.11 ± 0.341
0.513MetHis: 0.513 ± 0.163
0.798MetIle: 0.798 ± 0.181
1.255MetLys: 1.255 ± 0.242
2.11MetLeu: 2.11 ± 0.375
0.798MetMet: 0.798 ± 0.166
0.855MetAsn: 0.855 ± 0.207
1.768MetPro: 1.768 ± 0.264
1.597MetGln: 1.597 ± 0.374
2.053MetArg: 2.053 ± 0.335
2.11MetSer: 2.11 ± 0.383
1.996MetThr: 1.996 ± 0.349
1.312MetVal: 1.312 ± 0.303
0.285MetTrp: 0.285 ± 0.114
0.627MetTyr: 0.627 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
4.962AsnAla: 4.962 ± 0.506
0.171AsnCys: 0.171 ± 0.096
2.11AsnAsp: 2.11 ± 0.329
1.711AsnGlu: 1.711 ± 0.305
0.684AsnPhe: 0.684 ± 0.246
4.049AsnGly: 4.049 ± 0.662
0.741AsnHis: 0.741 ± 0.231
1.255AsnIle: 1.255 ± 0.365
1.654AsnLys: 1.654 ± 0.318
2.167AsnLeu: 2.167 ± 0.351
1.084AsnMet: 1.084 ± 0.29
1.255AsnAsn: 1.255 ± 0.279
2.395AsnPro: 2.395 ± 0.41
1.255AsnGln: 1.255 ± 0.255
2.452AsnArg: 2.452 ± 0.326
2.11AsnSer: 2.11 ± 0.374
2.281AsnThr: 2.281 ± 0.401
2.566AsnVal: 2.566 ± 0.43
0.741AsnTrp: 0.741 ± 0.231
0.57AsnTyr: 0.57 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
8.839ProAla: 8.839 ± 1.386
0.513ProCys: 0.513 ± 0.205
2.68ProAsp: 2.68 ± 0.319
3.422ProGlu: 3.422 ± 0.495
1.312ProPhe: 1.312 ± 0.29
4.448ProGly: 4.448 ± 0.704
0.798ProHis: 0.798 ± 0.22
1.825ProIle: 1.825 ± 0.342
2.509ProLys: 2.509 ± 0.373
2.851ProLeu: 2.851 ± 0.364
0.912ProMet: 0.912 ± 0.225
1.255ProAsn: 1.255 ± 0.249
2.167ProPro: 2.167 ± 0.351
1.825ProGln: 1.825 ± 0.488
2.965ProArg: 2.965 ± 0.407
3.821ProSer: 3.821 ± 0.558
3.536ProThr: 3.536 ± 0.466
3.023ProVal: 3.023 ± 0.411
0.342ProTrp: 0.342 ± 0.123
0.912ProTyr: 0.912 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
6.729GlnAla: 6.729 ± 0.723
0.057GlnCys: 0.057 ± 0.053
1.882GlnAsp: 1.882 ± 0.34
2.452GlnGlu: 2.452 ± 0.408
0.855GlnPhe: 0.855 ± 0.223
2.737GlnGly: 2.737 ± 0.493
1.084GlnHis: 1.084 ± 0.321
1.825GlnIle: 1.825 ± 0.41
1.483GlnLys: 1.483 ± 0.436
3.023GlnLeu: 3.023 ± 0.325
1.198GlnMet: 1.198 ± 0.405
1.312GlnAsn: 1.312 ± 0.281
1.882GlnPro: 1.882 ± 0.328
3.479GlnGln: 3.479 ± 0.587
1.882GlnArg: 1.882 ± 0.447
1.939GlnSer: 1.939 ± 0.32
1.711GlnThr: 1.711 ± 0.31
3.308GlnVal: 3.308 ± 0.384
0.741GlnTrp: 0.741 ± 0.207
1.027GlnTyr: 1.027 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
9.182ArgAla: 9.182 ± 0.802
0.456ArgCys: 0.456 ± 0.182
4.505ArgAsp: 4.505 ± 0.502
4.505ArgGlu: 4.505 ± 0.643
2.965ArgPhe: 2.965 ± 0.498
4.448ArgGly: 4.448 ± 0.519
1.255ArgHis: 1.255 ± 0.289
3.308ArgIle: 3.308 ± 0.45
3.422ArgLys: 3.422 ± 0.418
5.874ArgLeu: 5.874 ± 0.631
2.509ArgMet: 2.509 ± 0.289
2.737ArgAsn: 2.737 ± 0.373
2.737ArgPro: 2.737 ± 0.496
3.365ArgGln: 3.365 ± 0.612
4.847ArgArg: 4.847 ± 0.655
2.965ArgSer: 2.965 ± 0.42
2.965ArgThr: 2.965 ± 0.477
4.619ArgVal: 4.619 ± 0.627
1.027ArgTrp: 1.027 ± 0.278
2.338ArgTyr: 2.338 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
7.015SerAla: 7.015 ± 0.992
0.456SerCys: 0.456 ± 0.223
3.65SerAsp: 3.65 ± 0.368
2.851SerGlu: 2.851 ± 0.361
1.255SerPhe: 1.255 ± 0.279
5.475SerGly: 5.475 ± 0.579
1.54SerHis: 1.54 ± 0.314
2.68SerIle: 2.68 ± 0.319
2.851SerLys: 2.851 ± 0.333
3.935SerLeu: 3.935 ± 0.436
1.312SerMet: 1.312 ± 0.309
2.224SerAsn: 2.224 ± 0.37
2.851SerPro: 2.851 ± 0.374
2.509SerGln: 2.509 ± 0.375
3.422SerArg: 3.422 ± 0.451
2.965SerSer: 2.965 ± 0.328
3.65SerThr: 3.65 ± 0.43
3.479SerVal: 3.479 ± 0.506
0.969SerTrp: 0.969 ± 0.227
1.198SerTyr: 1.198 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
7.015ThrAla: 7.015 ± 0.661
0.456ThrCys: 0.456 ± 0.24
2.338ThrAsp: 2.338 ± 0.346
3.479ThrGlu: 3.479 ± 0.471
1.426ThrPhe: 1.426 ± 0.263
5.931ThrGly: 5.931 ± 0.757
1.597ThrHis: 1.597 ± 0.346
3.08ThrIle: 3.08 ± 0.48
2.965ThrLys: 2.965 ± 0.325
4.22ThrLeu: 4.22 ± 0.544
1.255ThrMet: 1.255 ± 0.239
1.711ThrAsn: 1.711 ± 0.457
3.023ThrPro: 3.023 ± 0.364
1.54ThrGln: 1.54 ± 0.359
3.536ThrArg: 3.536 ± 0.57
2.623ThrSer: 2.623 ± 0.548
2.965ThrThr: 2.965 ± 0.482
3.992ThrVal: 3.992 ± 0.399
0.627ThrTrp: 0.627 ± 0.252
1.369ThrTyr: 1.369 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
8.326ValAla: 8.326 ± 0.645
0.741ValCys: 0.741 ± 0.228
3.935ValAsp: 3.935 ± 0.546
3.707ValGlu: 3.707 ± 0.493
1.882ValPhe: 1.882 ± 0.496
4.562ValGly: 4.562 ± 0.445
0.912ValHis: 0.912 ± 0.26
2.737ValIle: 2.737 ± 0.61
3.023ValLys: 3.023 ± 0.596
3.365ValLeu: 3.365 ± 0.384
1.654ValMet: 1.654 ± 0.243
2.737ValAsn: 2.737 ± 0.424
4.448ValPro: 4.448 ± 0.514
2.11ValGln: 2.11 ± 0.343
4.562ValArg: 4.562 ± 0.519
4.277ValSer: 4.277 ± 0.477
3.707ValThr: 3.707 ± 0.475
3.992ValVal: 3.992 ± 0.504
0.684ValTrp: 0.684 ± 0.226
1.654ValTyr: 1.654 ± 0.318
0.0ValXaa: 0.0 ± 0.0
Trp
1.198TrpAla: 1.198 ± 0.298
0.114TrpCys: 0.114 ± 0.082
0.741TrpAsp: 0.741 ± 0.246
0.627TrpGlu: 0.627 ± 0.233
0.684TrpPhe: 0.684 ± 0.208
0.969TrpGly: 0.969 ± 0.25
0.285TrpHis: 0.285 ± 0.146
0.684TrpIle: 0.684 ± 0.201
0.513TrpLys: 0.513 ± 0.192
1.369TrpLeu: 1.369 ± 0.269
0.513TrpMet: 0.513 ± 0.141
0.57TrpAsn: 0.57 ± 0.182
0.285TrpPro: 0.285 ± 0.138
0.513TrpGln: 0.513 ± 0.185
1.369TrpArg: 1.369 ± 0.333
1.027TrpSer: 1.027 ± 0.264
0.912TrpThr: 0.912 ± 0.204
1.084TrpVal: 1.084 ± 0.235
0.114TrpTrp: 0.114 ± 0.08
0.342TrpTyr: 0.342 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.794TyrAla: 2.794 ± 0.373
0.456TyrCys: 0.456 ± 0.171
2.11TyrAsp: 2.11 ± 0.217
1.54TyrGlu: 1.54 ± 0.262
0.969TyrPhe: 0.969 ± 0.269
2.11TyrGly: 2.11 ± 0.463
0.342TyrHis: 0.342 ± 0.147
1.027TyrIle: 1.027 ± 0.271
0.969TyrLys: 0.969 ± 0.186
2.053TyrLeu: 2.053 ± 0.425
0.285TyrMet: 0.285 ± 0.104
1.084TyrAsn: 1.084 ± 0.253
0.798TyrPro: 0.798 ± 0.18
0.798TyrGln: 0.798 ± 0.181
2.224TyrArg: 2.224 ± 0.323
1.426TyrSer: 1.426 ± 0.389
1.54TyrThr: 1.54 ± 0.306
1.426TyrVal: 1.426 ± 0.231
0.399TyrTrp: 0.399 ± 0.269
0.57TyrTyr: 0.57 ± 0.148
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (17536 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski