Muricauda lutaonensis
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2882 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5YVH3|A0A0D5YVH3_9FLAO Uncharacterized protein OS=Muricauda lutaonensis OX=516051 GN=VC82_2257 PE=4 SV=1
MM1 pKa = 7.49 GKK3 pKa = 8.33 MLKK6 pKa = 9.95 IFVLFFLLTISIANAQDD23 pKa = 3.15 TYY25 pKa = 11.34 YY26 pKa = 10.24 DD27 pKa = 3.72 TFSSVSYY34 pKa = 10.67 SNNDD38 pKa = 2.73 GTQNWSTNWIEE49 pKa = 4.62 SGDD52 pKa = 4.03 DD53 pKa = 3.46 NSPSGGYY60 pKa = 9.44 IRR62 pKa = 11.84 ITGNQLRR69 pKa = 11.84 FAYY72 pKa = 9.21 IWTEE76 pKa = 3.95 TIRR79 pKa = 11.84 RR80 pKa = 11.84 SADD83 pKa = 2.89 LSAYY87 pKa = 10.5 SSATLSFDD95 pKa = 2.93 WQTSSLEE102 pKa = 4.13 SGEE105 pKa = 4.19 TLDD108 pKa = 4.32 VQVSSDD114 pKa = 3.04 GSSYY118 pKa = 7.99 TTLATFSGNQSGTFSQNISAHH139 pKa = 5.8 ISSNTTIRR147 pKa = 11.84 FIKK150 pKa = 10.4 GGNNWSGNNDD160 pKa = 2.74 RR161 pKa = 11.84 VYY163 pKa = 10.71 IDD165 pKa = 3.91 NVLITATATASVSIADD181 pKa = 3.55 VSVNEE186 pKa = 4.27 GDD188 pKa = 3.54 GTATFMVQHH197 pKa = 6.47 VGPNATGAFTVNFTTNDD214 pKa = 3.2 GTAVAGSDD222 pKa = 3.56 YY223 pKa = 9.81 TASSGTLSFNGTSGDD238 pKa = 3.86 TEE240 pKa = 5.27 SITVPINDD248 pKa = 4.52 DD249 pKa = 3.56 GDD251 pKa = 4.15 FEE253 pKa = 4.46 GLEE256 pKa = 4.24 TFTVSFTGTSDD267 pKa = 3.23 GTINISDD274 pKa = 4.01 TATGSITDD282 pKa = 3.94 NDD284 pKa = 4.33 LLGNTPLALFEE295 pKa = 5.23 AFDD298 pKa = 4.64 GYY300 pKa = 11.75 VDD302 pKa = 3.73 YY303 pKa = 10.63 TSTGGTLRR311 pKa = 11.84 TQPNTGDD318 pKa = 3.02 ACAITTSSSNTLTSSIPATATIQKK342 pKa = 10.6 ALLYY346 pKa = 7.77 WAHH349 pKa = 6.44 SAATPDD355 pKa = 3.46 SQVTFEE361 pKa = 4.74 GNTVTADD368 pKa = 3.47 LMYY371 pKa = 7.82 TTTLTNRR378 pKa = 11.84 VFFGGVSDD386 pKa = 3.7 VTSIVQGISNPSTNTYY402 pKa = 11.07 DD403 pKa = 3.75 FTDD406 pKa = 3.63 LTVDD410 pKa = 3.25 NTGNYY415 pKa = 9.69 CSTATVLGGWSLFIFYY431 pKa = 10.47 EE432 pKa = 4.43 DD433 pKa = 3.65 ASLPASTVNLYY444 pKa = 10.58 QGFNGEE450 pKa = 4.72 SNSSSSFSLSGFFAIGASGSKK471 pKa = 7.02 TTVLSWEE478 pKa = 4.35 GDD480 pKa = 3.31 QTLSNNEE487 pKa = 4.26 LLTVTTGLGTFTLAGDD503 pKa = 3.95 GDD505 pKa = 4.49 NNGVAVNNPFNSTIFDD521 pKa = 3.34 NTIIPNVNNASAYY534 pKa = 10.45 GLDD537 pKa = 3.84 LDD539 pKa = 4.69 TYY541 pKa = 10.88 DD542 pKa = 3.65 VSPYY546 pKa = 9.34 IQPGEE551 pKa = 4.33 STVTTNVQSGQDD563 pKa = 3.7 FVIMNALVLKK573 pKa = 10.55 VPSNLITGTVFEE585 pKa = 4.77 DD586 pKa = 3.34 VNYY589 pKa = 11.09 GGGAGRR595 pKa = 11.84 NRR597 pKa = 11.84 ASSSGVGVQGATVEE611 pKa = 5.45 LYY613 pKa = 10.24 DD614 pKa = 4.31 ALNNFVDD621 pKa = 4.06 NTTTDD626 pKa = 2.97 ANGLYY631 pKa = 10.26 RR632 pKa = 11.84 FGGMANGSYY641 pKa = 9.93 SVRR644 pKa = 11.84 VVNGTVRR651 pKa = 11.84 STRR654 pKa = 11.84 GGGAACNTCWPVQTFRR670 pKa = 11.84 TSFPGGSVIEE680 pKa = 4.33 VTNEE684 pKa = 3.38 VGGADD689 pKa = 3.81 PSAEE693 pKa = 3.89 DD694 pKa = 3.55 SAAGTLTGAQSVAPVLITSEE714 pKa = 4.62 GVVGMDD720 pKa = 3.81 FGYY723 pKa = 11.06 NFNTIVNTNEE733 pKa = 4.11 DD734 pKa = 3.71 GQGSLEE740 pKa = 3.96 QFIVNANNLDD750 pKa = 4.17 EE751 pKa = 5.45 IGLDD755 pKa = 3.1 IVANSIFDD763 pKa = 3.84 PAAGQDD769 pKa = 3.34 TSIFMIPSSSDD780 pKa = 3.21 PLGRR784 pKa = 11.84 TPDD787 pKa = 3.37 SNFASGYY794 pKa = 9.29 FDD796 pKa = 4.8 IFISDD801 pKa = 4.5 GNPLSDD807 pKa = 2.97 ITADD811 pKa = 3.19 NTFIDD816 pKa = 3.98 GRR818 pKa = 11.84 TQTAYY823 pKa = 10.84 SGDD826 pKa = 4.11 TNSGTIGAGGNPVGVSGSLLPIFEE850 pKa = 4.64 LPEE853 pKa = 4.12 IQVHH857 pKa = 6.33 RR858 pKa = 11.84 DD859 pKa = 3.0 GGDD862 pKa = 3.42 VLKK865 pKa = 11.1 VQADD869 pKa = 3.65 VVLRR873 pKa = 11.84 NLSIYY878 pKa = 10.79 ADD880 pKa = 3.64 NTAGIQVGSGSAVIEE895 pKa = 4.13 RR896 pKa = 11.84 NLIGVNAQGTNSGNINYY913 pKa = 10.03 GIEE916 pKa = 4.3 NIGGSILIDD925 pKa = 3.41 GNYY928 pKa = 9.66 IATNTDD934 pKa = 2.2 AGIFVNGGTTNIIQNNHH951 pKa = 4.4 ITNNGNSACTDD962 pKa = 3.63 NILVQGGSGLVITQNFIEE980 pKa = 4.47 NAASAGIDD988 pKa = 3.28 ASASSGNITISEE1000 pKa = 4.06 NSITASGQDD1009 pKa = 3.42 GGNCSGNIEE1018 pKa = 3.98 GMAIKK1023 pKa = 10.26 IGGNNSVVTNNMIYY1037 pKa = 10.77 ANGGAGIVVVGGTANRR1053 pKa = 11.84 ISQNSIYY1060 pKa = 11.06 ANGTISDD1067 pKa = 4.14 ALGIDD1072 pKa = 4.48 LDD1074 pKa = 4.2 ASSGAGDD1081 pKa = 4.12 GVTLNDD1087 pKa = 4.52 NGDD1090 pKa = 3.74 GDD1092 pKa = 4.15 TGPNDD1097 pKa = 3.7 LQNFPIISAAYY1108 pKa = 8.58 ISGSNLVVKK1117 pKa = 10.34 GWSRR1121 pKa = 11.84 PGATIEE1127 pKa = 5.38 FFFTDD1132 pKa = 3.6 INEE1135 pKa = 4.34 GTATTGDD1142 pKa = 3.83 NQLGMAVDD1150 pKa = 3.8 YY1151 pKa = 11.67 GEE1153 pKa = 4.39 GQTFIGAAVEE1163 pKa = 4.33 GSGADD1168 pKa = 3.46 ADD1170 pKa = 4.29 NTSSSYY1176 pKa = 11.66 SDD1178 pKa = 3.31 VDD1180 pKa = 3.85 GNTDD1184 pKa = 2.99 NTNRR1188 pKa = 11.84 FHH1190 pKa = 7.56 FNLPVPSGATLGEE1203 pKa = 4.73 MITATATLGNSTSEE1217 pKa = 4.18 FAPMSILRR1225 pKa = 11.84 AATVITNRR1233 pKa = 11.84 RR1234 pKa = 11.84 ITYY1237 pKa = 9.66 RR1238 pKa = 11.84 VSKK1241 pKa = 10.76 NN1242 pKa = 3.0
Molecular weight: 128.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.681
IPC_protein 3.745
Toseland 3.503
ProMoST 3.91
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.859
Patrickios 1.036
IPC_peptide 3.732
IPC2_peptide 3.834
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|A0A0D5YRP8|A0A0D5YRP8_9FLAO Uncharacterized protein OS=Muricauda lutaonensis OX=516051 GN=VC82_1328 PE=4 SV=1
MM1 pKa = 7.46 NEE3 pKa = 3.5 VSLLDD8 pKa = 3.32 WFLAFTAIFLLGISKK23 pKa = 10.51 SGLKK27 pKa = 10.23 GLSIFNVTLLALAFGARR44 pKa = 11.84 EE45 pKa = 4.05 STGILIPLLLVGDD58 pKa = 4.35 VFAVLYY64 pKa = 9.15 YY65 pKa = 10.44 NRR67 pKa = 11.84 HH68 pKa = 4.39 TQWRR72 pKa = 11.84 YY73 pKa = 9.19 ILRR76 pKa = 11.84 FLPWMISGILIGVFLGKK93 pKa = 9.89 NLPEE97 pKa = 4.12 TQFKK101 pKa = 10.11 IGMVIVIFISLGVLTYY117 pKa = 9.61 WDD119 pKa = 3.4 RR120 pKa = 11.84 RR121 pKa = 11.84 KK122 pKa = 10.3 SKK124 pKa = 10.37 KK125 pKa = 10.44 VPTHH129 pKa = 4.16 WTFAGGVGILAGICTMIGNLAGAFTNIFFLAMRR162 pKa = 11.84 LPKK165 pKa = 10.57 NEE167 pKa = 4.14 FVGTAAWLFLITNLFKK183 pKa = 11.08 LPFHH187 pKa = 5.87 IWVWKK192 pKa = 8.77 TITPEE197 pKa = 3.93 TVGINLKK204 pKa = 10.0 LLPAILVGLAIGVRR218 pKa = 11.84 IVRR221 pKa = 11.84 RR222 pKa = 11.84 INEE225 pKa = 3.62 KK226 pKa = 8.96 QYY228 pKa = 10.98 RR229 pKa = 11.84 RR230 pKa = 11.84 FILIVTAIGAVVILLRR246 pKa = 4.58
Molecular weight: 27.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.882
IPC_protein 10.57
Toseland 10.833
ProMoST 10.628
Dawson 10.921
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.272
Grimsley 10.965
Solomon 11.008
Lehninger 10.979
Nozaki 10.804
DTASelect 10.584
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 10.994
IPC_peptide 11.023
IPC2_peptide 9.385
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2882
0
2882
971754
50
4585
337.2
37.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.973 ± 0.047
0.746 ± 0.017
5.839 ± 0.055
6.859 ± 0.043
5.143 ± 0.037
6.989 ± 0.053
1.954 ± 0.03
6.91 ± 0.041
7.198 ± 0.079
9.491 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.324 ± 0.028
5.332 ± 0.049
3.748 ± 0.028
3.475 ± 0.026
4.09 ± 0.038
5.798 ± 0.036
5.508 ± 0.066
6.663 ± 0.034
1.125 ± 0.019
3.832 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here