Spider associated circular virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus cybusi1

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346BP71|A0A346BP71_9VIRU Putative capsid protein OS=Spider associated circular virus 1 OX=2293302 PE=4 SV=1
MM1 pKa = 7.67PFVFQARR8 pKa = 11.84YY9 pKa = 9.56CLLTFSQCDD18 pKa = 3.24ALDD21 pKa = 3.63GFRR24 pKa = 11.84VMEE27 pKa = 4.42HH28 pKa = 6.58LSGLGAEE35 pKa = 4.68CIVGRR40 pKa = 11.84EE41 pKa = 3.74IHH43 pKa = 6.84ADD45 pKa = 3.35GGIHH49 pKa = 6.09LHH51 pKa = 6.41CFVDD55 pKa = 4.62FGRR58 pKa = 11.84KK59 pKa = 8.02FRR61 pKa = 11.84SRR63 pKa = 11.84KK64 pKa = 7.67TDD66 pKa = 2.7IFDD69 pKa = 3.24VDD71 pKa = 3.45GRR73 pKa = 11.84HH74 pKa = 5.78PNIVGSYY81 pKa = 5.99GTPWGGYY88 pKa = 10.08DD89 pKa = 3.67YY90 pKa = 10.8AIKK93 pKa = 10.91DD94 pKa = 3.47GDD96 pKa = 4.3VVCGGLEE103 pKa = 3.84RR104 pKa = 11.84PEE106 pKa = 3.88QPRR109 pKa = 11.84SKK111 pKa = 10.36RR112 pKa = 11.84VTKK115 pKa = 10.57DD116 pKa = 2.12WDD118 pKa = 2.91AWTEE122 pKa = 3.71ITNARR127 pKa = 11.84DD128 pKa = 3.58RR129 pKa = 11.84EE130 pKa = 4.49HH131 pKa = 6.41FWEE134 pKa = 4.87LVHH137 pKa = 8.03HH138 pKa = 7.16LDD140 pKa = 4.88PKK142 pKa = 10.84AAACNHH148 pKa = 5.45GQLAKK153 pKa = 10.36YY154 pKa = 10.07ADD156 pKa = 2.88WRR158 pKa = 11.84FRR160 pKa = 11.84VKK162 pKa = 10.18PAVYY166 pKa = 8.99EE167 pKa = 4.32SPGGVSFVGGDD178 pKa = 3.29ADD180 pKa = 6.29GRR182 pKa = 11.84DD183 pKa = 3.48AWCDD187 pKa = 3.17QSGIRR192 pKa = 11.84SGEE195 pKa = 3.87PLVGSEE201 pKa = 4.89LKK203 pKa = 10.46KK204 pKa = 11.0ASNDD208 pKa = 3.4DD209 pKa = 3.12VKK211 pKa = 11.37YY212 pKa = 10.88AIFDD216 pKa = 4.73DD217 pKa = 3.41IRR219 pKa = 11.84GGIKK223 pKa = 9.97FFPAFKK229 pKa = 9.97EE230 pKa = 3.97WLGAQAFVTVKK241 pKa = 10.2EE242 pKa = 4.57LYY244 pKa = 10.14RR245 pKa = 11.84EE246 pKa = 4.05PALMKK251 pKa = 9.62WGKK254 pKa = 9.54PSIWLSNEE262 pKa = 3.48DD263 pKa = 3.31PRR265 pKa = 11.84NYY267 pKa = 9.56MEE269 pKa = 4.36QSDD272 pKa = 4.05RR273 pKa = 11.84TWLEE277 pKa = 3.88EE278 pKa = 3.62NCVFIEE284 pKa = 3.64ITEE287 pKa = 4.72PIFRR291 pKa = 11.84ASII294 pKa = 3.49

Molecular weight: 33.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346BP71|A0A346BP71_9VIRU Putative capsid protein OS=Spider associated circular virus 1 OX=2293302 PE=4 SV=1
MM1 pKa = 7.85AFRR4 pKa = 11.84IRR6 pKa = 11.84APRR9 pKa = 11.84GRR11 pKa = 11.84YY12 pKa = 8.61RR13 pKa = 11.84RR14 pKa = 11.84PTSRR18 pKa = 11.84AAKK21 pKa = 9.56KK22 pKa = 10.34SYY24 pKa = 9.96GGSRR28 pKa = 11.84SRR30 pKa = 11.84RR31 pKa = 11.84SYY33 pKa = 10.09RR34 pKa = 11.84KK35 pKa = 8.97KK36 pKa = 8.43RR37 pKa = 11.84TYY39 pKa = 10.48RR40 pKa = 11.84KK41 pKa = 9.52RR42 pKa = 11.84KK43 pKa = 8.7SPRR46 pKa = 11.84KK47 pKa = 9.75AILNLTSTKK56 pKa = 10.2KK57 pKa = 10.43RR58 pKa = 11.84NTMLQYY64 pKa = 11.57ANTSSVNGTSVNIGPGPYY82 pKa = 9.62LVNGATGGFSIFCPTAMDD100 pKa = 3.69LTEE103 pKa = 5.03KK104 pKa = 10.62SGANNSVVNVAEE116 pKa = 4.51RR117 pKa = 11.84NSTTCFMRR125 pKa = 11.84GFSEE129 pKa = 4.3DD130 pKa = 3.99LRR132 pKa = 11.84VQTSTGVPWFWRR144 pKa = 11.84RR145 pKa = 11.84ITFCSKK151 pKa = 9.84AGGVFGVFFAGDD163 pKa = 3.7TPTQTNGGEE172 pKa = 4.07TSYY175 pKa = 11.37TDD177 pKa = 3.16TSNGMEE183 pKa = 4.5RR184 pKa = 11.84IWFNQNVNNAPLTVTGIQAILFKK207 pKa = 11.09GVINKK212 pKa = 9.65DD213 pKa = 2.8WVDD216 pKa = 3.72PLTAPLDD223 pKa = 3.65TARR226 pKa = 11.84IDD228 pKa = 3.57VKK230 pKa = 11.32SDD232 pKa = 2.93VTKK235 pKa = 10.9CIRR238 pKa = 11.84SGNASGTVMQQKK250 pKa = 8.65CWYY253 pKa = 8.45PMNKK257 pKa = 9.47NLVYY261 pKa = 10.81DD262 pKa = 4.24DD263 pKa = 5.38DD264 pKa = 4.33EE265 pKa = 6.69AGEE268 pKa = 4.6MEE270 pKa = 4.33VSTYY274 pKa = 10.58TSVVDD279 pKa = 3.34KK280 pKa = 10.99RR281 pKa = 11.84GMGDD285 pKa = 3.32YY286 pKa = 10.81YY287 pKa = 10.26IIDD290 pKa = 3.79IFTVGTGGGTADD302 pKa = 4.29LLQVTSTSSIYY313 pKa = 8.98WHH315 pKa = 6.78EE316 pKa = 4.14KK317 pKa = 9.12

Molecular weight: 35.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

611

294

317

305.5

34.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.511

2.128 ± 0.392

6.71 ± 1.186

4.746 ± 1.36

5.074 ± 0.693

9.493 ± 0.021

1.637 ± 0.942

5.074 ± 0.244

5.728 ± 0.189

4.91 ± 0.577

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.128 ± 0.507

4.419 ± 1.347

4.255 ± 0.335

2.455 ± 0.049

7.529 ± 0.255

6.874 ± 1.172

7.038 ± 2.629

6.874 ± 0.272

2.619 ± 0.517

3.601 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski