Pseudonocardiaceae bacterium YIM PH 21723

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; unclassified Pseudonocardiaceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7085 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419HHC9|A0A419HHC9_9PSEU Uncharacterized protein OS=Pseudonocardiaceae bacterium YIM PH 21723 OX=2340915 GN=D5S17_22630 PE=4 SV=1
MM1 pKa = 7.44TLMNRR6 pKa = 11.84YY7 pKa = 7.44TDD9 pKa = 3.44TFEE12 pKa = 4.22EE13 pKa = 4.42QVSIALAQLYY23 pKa = 8.97PEE25 pKa = 4.09WSGAFDD31 pKa = 5.93ASDD34 pKa = 3.19DD35 pKa = 3.65WPRR38 pKa = 11.84LVSVLDD44 pKa = 4.05DD45 pKa = 4.14LCDD48 pKa = 3.39HH49 pKa = 6.65GRR51 pKa = 11.84AKK53 pKa = 11.04DD54 pKa = 3.69PFTVLDD60 pKa = 4.98DD61 pKa = 4.92LDD63 pKa = 5.35HH64 pKa = 6.76DD65 pKa = 3.5TWDD68 pKa = 3.4RR69 pKa = 11.84MKK71 pKa = 11.09AGDD74 pKa = 4.23DD75 pKa = 3.58VATEE79 pKa = 4.84LIAILTGQPHH89 pKa = 6.32EE90 pKa = 5.08CDD92 pKa = 3.28GCDD95 pKa = 2.72WCLSYY100 pKa = 10.63IGEE103 pKa = 4.48DD104 pKa = 3.42LAA106 pKa = 6.58

Molecular weight: 11.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419H9Y8|A0A419H9Y8_9PSEU AAA_31 domain-containing protein OS=Pseudonocardiaceae bacterium YIM PH 21723 OX=2340915 GN=D5S17_35530 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84GKK40 pKa = 10.43GRR42 pKa = 11.84ASLSAA47 pKa = 3.83

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7085

0

7085

2221496

24

8627

313.5

33.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.346 ± 0.035

0.87 ± 0.009

5.912 ± 0.025

5.454 ± 0.028

2.93 ± 0.014

9.061 ± 0.03

2.233 ± 0.016

4.021 ± 0.019

2.447 ± 0.021

10.763 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.013

2.185 ± 0.015

5.75 ± 0.024

3.365 ± 0.019

7.417 ± 0.031

5.312 ± 0.019

6.084 ± 0.021

8.265 ± 0.028

1.606 ± 0.013

2.122 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski