Amino acid dipepetide frequency for Pseudonocardiaceae bacterium YIM PH 21723

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.586AlaAla: 17.586 ± 0.126	1.03AlaCys: 1.03 ± 0.02	7.811AlaAsp: 7.811 ± 0.055	8.384AlaGlu: 8.384 ± 0.097	3.305AlaPhe: 3.305 ± 0.036	12.145AlaGly: 12.145 ± 0.097	2.385AlaHis: 2.385 ± 0.036	4.885AlaIle: 4.885 ± 0.055	3.437AlaLys: 3.437 ± 0.048	12.998AlaLeu: 12.998 ± 0.099	2.592AlaMet: 2.592 ± 0.037	2.388AlaAsn: 2.388 ± 0.037	5.559AlaPro: 5.559 ± 0.067	4.135AlaGln: 4.135 ± 0.042	7.876AlaArg: 7.876 ± 0.063	5.251AlaSer: 5.251 ± 0.051	6.993AlaThr: 6.993 ± 0.065	10.672AlaVal: 10.672 ± 0.075	1.71AlaTrp: 1.71 ± 0.029	2.312AlaTyr: 2.312 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.084CysAla: 1.084 ± 0.021	0.106CysCys: 0.106 ± 0.007	0.53CysAsp: 0.53 ± 0.016	0.385CysGlu: 0.385 ± 0.016	0.253CysPhe: 0.253 ± 0.011	1.015CysGly: 1.015 ± 0.023	0.197CysHis: 0.197 ± 0.009	0.209CysIle: 0.209 ± 0.008	0.172CysLys: 0.172 ± 0.009	0.796CysLeu: 0.796 ± 0.021	0.16CysMet: 0.16 ± 0.009	0.19CysAsn: 0.19 ± 0.01	0.538CysPro: 0.538 ± 0.016	0.264CysGln: 0.264 ± 0.013	0.646CysArg: 0.646 ± 0.019	0.553CysSer: 0.553 ± 0.016	0.585CysThr: 0.585 ± 0.015	0.683CysVal: 0.683 ± 0.015	0.152CysTrp: 0.152 ± 0.008	0.181CysTyr: 0.181 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.438AspAla: 6.438 ± 0.055	0.499AspCys: 0.499 ± 0.015	2.998AspAsp: 2.998 ± 0.04	3.699AspGlu: 3.699 ± 0.05	1.715AspPhe: 1.715 ± 0.028	5.843AspGly: 5.843 ± 0.056	1.454AspHis: 1.454 ± 0.026	2.197AspIle: 2.197 ± 0.03	1.483AspLys: 1.483 ± 0.027	6.536AspLeu: 6.536 ± 0.056	0.922AspMet: 0.922 ± 0.021	1.164AspAsn: 1.164 ± 0.023	4.241AspPro: 4.241 ± 0.044	2.24AspGln: 2.24 ± 0.035	5.043AspArg: 5.043 ± 0.054	2.811AspSer: 2.811 ± 0.036	3.043AspThr: 3.043 ± 0.037	4.388AspVal: 4.388 ± 0.044	1.076AspTrp: 1.076 ± 0.022	1.335AspTyr: 1.335 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	6.018GluAla: 6.018 ± 0.066	0.39GluCys: 0.39 ± 0.013	2.622GluAsp: 2.622 ± 0.035	2.624GluGlu: 2.624 ± 0.041	1.908GluPhe: 1.908 ± 0.029	3.291GluGly: 3.291 ± 0.041	1.675GluHis: 1.675 ± 0.026	2.588GluIle: 2.588 ± 0.037	1.372GluLys: 1.372 ± 0.029	7.791GluLeu: 7.791 ± 0.067	0.911GluMet: 0.911 ± 0.019	1.173GluAsn: 1.173 ± 0.024	3.186GluPro: 3.186 ± 0.052	2.786GluGln: 2.786 ± 0.041	4.549GluArg: 4.549 ± 0.056	2.576GluSer: 2.576 ± 0.036	2.639GluThr: 2.639 ± 0.039	4.467GluVal: 4.467 ± 0.056	0.78GluTrp: 0.78 ± 0.021	1.193GluTyr: 1.193 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.803PheAla: 3.803 ± 0.043	0.318PheCys: 0.318 ± 0.012	2.186PheAsp: 2.186 ± 0.034	1.44PheGlu: 1.44 ± 0.025	0.912PhePhe: 0.912 ± 0.022	3.297PheGly: 3.297 ± 0.049	0.665PheHis: 0.665 ± 0.02	0.94PheIle: 0.94 ± 0.024	0.536PheLys: 0.536 ± 0.015	2.754PheLeu: 2.754 ± 0.041	0.408PheMet: 0.408 ± 0.015	0.641PheAsn: 0.641 ± 0.02	1.467PhePro: 1.467 ± 0.03	0.908PheGln: 0.908 ± 0.021	1.815PheArg: 1.815 ± 0.03	1.639PheSer: 1.639 ± 0.026	2.175PheThr: 2.175 ± 0.032	2.267PheVal: 2.267 ± 0.034	0.465PheTrp: 0.465 ± 0.014	0.665PheTyr: 0.665 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	9.354GlyAla: 9.354 ± 0.08	0.937GlyCys: 0.937 ± 0.02	4.899GlyAsp: 4.899 ± 0.048	4.82GlyGlu: 4.82 ± 0.044	3.132GlyPhe: 3.132 ± 0.043	7.995GlyGly: 7.995 ± 0.1	2.252GlyHis: 2.252 ± 0.031	3.938GlyIle: 3.938 ± 0.051	2.871GlyLys: 2.871 ± 0.04	9.668GlyLeu: 9.668 ± 0.083	2.147GlyMet: 2.147 ± 0.032	2.123GlyAsn: 2.123 ± 0.051	4.44GlyPro: 4.44 ± 0.05	3.223GlyGln: 3.223 ± 0.041	6.442GlyArg: 6.442 ± 0.058	5.303GlySer: 5.303 ± 0.061	5.664GlyThr: 5.664 ± 0.056	7.126GlyVal: 7.126 ± 0.061	1.8GlyTrp: 1.8 ± 0.032	2.475GlyTyr: 2.475 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.411HisAla: 2.411 ± 0.035	0.265HisCys: 0.265 ± 0.011	1.253HisAsp: 1.253 ± 0.026	1.156HisGlu: 1.156 ± 0.024	0.62HisPhe: 0.62 ± 0.019	2.21HisGly: 2.21 ± 0.036	0.65HisHis: 0.65 ± 0.02	0.783HisIle: 0.783 ± 0.021	0.403HisLys: 0.403 ± 0.014	2.319HisLeu: 2.319 ± 0.035	0.357HisMet: 0.357 ± 0.014	0.47HisAsn: 0.47 ± 0.013	1.651HisPro: 1.651 ± 0.03	0.758HisGln: 0.758 ± 0.019	2.131HisArg: 2.131 ± 0.033	1.189HisSer: 1.189 ± 0.024	1.235HisThr: 1.235 ± 0.026	1.558HisVal: 1.558 ± 0.026	0.399HisTrp: 0.399 ± 0.013	0.512HisTyr: 0.512 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.625IleAla: 5.625 ± 0.049	0.369IleCys: 0.369 ± 0.014	2.887IleAsp: 2.887 ± 0.038	2.358IleGlu: 2.358 ± 0.034	0.938IlePhe: 0.938 ± 0.021	4.464IleGly: 4.464 ± 0.05	0.708IleHis: 0.708 ± 0.019	1.376IleIle: 1.376 ± 0.024	0.983IleLys: 0.983 ± 0.021	2.96IleLeu: 2.96 ± 0.04	0.616IleMet: 0.616 ± 0.017	1.057IleAsn: 1.057 ± 0.022	2.261IlePro: 2.261 ± 0.035	0.999IleGln: 0.999 ± 0.026	2.72IleArg: 2.72 ± 0.041	2.408IleSer: 2.408 ± 0.03	3.147IleThr: 3.147 ± 0.04	3.072IleVal: 3.072 ± 0.037	0.493IleTrp: 0.493 ± 0.016	0.767IleTyr: 0.767 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.22LysAla: 3.22 ± 0.043	0.139LysCys: 0.139 ± 0.008	1.457LysAsp: 1.457 ± 0.031	1.093LysGlu: 1.093 ± 0.023	0.633LysPhe: 0.633 ± 0.018	1.796LysGly: 1.796 ± 0.031	0.533LysHis: 0.533 ± 0.015	1.182LysIle: 1.182 ± 0.028	0.784LysLys: 0.784 ± 0.023	2.569LysLeu: 2.569 ± 0.037	0.432LysMet: 0.432 ± 0.016	0.63LysAsn: 0.63 ± 0.023	1.739LysPro: 1.739 ± 0.033	0.969LysGln: 0.969 ± 0.022	1.556LysArg: 1.556 ± 0.027	1.297LysSer: 1.297 ± 0.027	1.361LysThr: 1.361 ± 0.028	2.217LysVal: 2.217 ± 0.038	0.306LysTrp: 0.306 ± 0.013	0.559LysTyr: 0.559 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.763LeuAla: 14.763 ± 0.11	0.911LeuCys: 0.911 ± 0.019	6.839LeuAsp: 6.839 ± 0.063	4.62LeuGlu: 4.62 ± 0.055	2.795LeuPhe: 2.795 ± 0.041	9.588LeuGly: 9.588 ± 0.073	2.331LeuHis: 2.331 ± 0.036	4.482LeuIle: 4.482 ± 0.049	2.051LeuLys: 2.051 ± 0.036	12.027LeuLeu: 12.027 ± 0.105	1.616LeuMet: 1.616 ± 0.03	2.251LeuAsn: 2.251 ± 0.031	6.593LeuPro: 6.593 ± 0.06	2.506LeuGln: 2.506 ± 0.033	8.757LeuArg: 8.757 ± 0.079	6.199LeuSer: 6.199 ± 0.047	6.981LeuThr: 6.981 ± 0.064	9.044LeuVal: 9.044 ± 0.076	1.394LeuTrp: 1.394 ± 0.026	1.88LeuTyr: 1.88 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.352MetAla: 2.352 ± 0.035	0.135MetCys: 0.135 ± 0.009	0.957MetAsp: 0.957 ± 0.018	0.7MetGlu: 0.7 ± 0.019	0.57MetPhe: 0.57 ± 0.015	1.319MetGly: 1.319 ± 0.025	0.381MetHis: 0.381 ± 0.013	0.898MetIle: 0.898 ± 0.021	0.443MetLys: 0.443 ± 0.014	1.963MetLeu: 1.963 ± 0.029	0.318MetMet: 0.318 ± 0.013	0.49MetAsn: 0.49 ± 0.015	1.142MetPro: 1.142 ± 0.02	0.508MetGln: 0.508 ± 0.015	1.534MetArg: 1.534 ± 0.027	1.38MetSer: 1.38 ± 0.026	1.506MetThr: 1.506 ± 0.024	1.453MetVal: 1.453 ± 0.029	0.243MetTrp: 0.243 ± 0.01	0.296MetTyr: 0.296 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.516AsnAla: 2.516 ± 0.042	0.241AsnCys: 0.241 ± 0.011	1.144AsnAsp: 1.144 ± 0.024	0.945AsnGlu: 0.945 ± 0.023	0.614AsnPhe: 0.614 ± 0.018	2.196AsnGly: 2.196 ± 0.043	0.473AsnHis: 0.473 ± 0.015	0.843AsnIle: 0.843 ± 0.023	0.578AsnLys: 0.578 ± 0.017	2.135AsnLeu: 2.135 ± 0.031	0.356AsnMet: 0.356 ± 0.01	0.573AsnAsn: 0.573 ± 0.018	1.768AsnPro: 1.768 ± 0.028	0.741AsnGln: 0.741 ± 0.02	1.615AsnArg: 1.615 ± 0.03	1.204AsnSer: 1.204 ± 0.024	1.363AsnThr: 1.363 ± 0.028	1.587AsnVal: 1.587 ± 0.028	0.413AsnTrp: 0.413 ± 0.014	0.549AsnTyr: 0.549 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	7.576ProAla: 7.576 ± 0.084	0.415ProCys: 0.415 ± 0.014	4.177ProAsp: 4.177 ± 0.051	4.137ProGlu: 4.137 ± 0.055	1.498ProPhe: 1.498 ± 0.025	6.123ProGly: 6.123 ± 0.071	1.149ProHis: 1.149 ± 0.025	1.98ProIle: 1.98 ± 0.032	1.454ProLys: 1.454 ± 0.031	5.202ProLeu: 5.202 ± 0.053	1.077ProMet: 1.077 ± 0.023	1.245ProAsn: 1.245 ± 0.026	2.96ProPro: 2.96 ± 0.051	2.043ProGln: 2.043 ± 0.034	3.206ProArg: 3.206 ± 0.038	2.857ProSer: 2.857 ± 0.039	3.049ProThr: 3.049 ± 0.044	5.368ProVal: 5.368 ± 0.053	0.919ProTrp: 0.919 ± 0.019	1.063ProTyr: 1.063 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	4.353GlnAla: 4.353 ± 0.054	0.234GlnCys: 0.234 ± 0.011	1.63GlnAsp: 1.63 ± 0.023	1.609GlnGlu: 1.609 ± 0.031	0.93GlnPhe: 0.93 ± 0.022	2.393GlnGly: 2.393 ± 0.036	0.831GlnHis: 0.831 ± 0.017	1.419GlnIle: 1.419 ± 0.027	0.673GlnLys: 0.673 ± 0.02	4.084GlnLeu: 4.084 ± 0.043	0.58GlnMet: 0.58 ± 0.017	0.705GlnAsn: 0.705 ± 0.017	2.153GlnPro: 2.153 ± 0.045	1.734GlnGln: 1.734 ± 0.035	2.905GlnArg: 2.905 ± 0.04	1.463GlnSer: 1.463 ± 0.024	1.528GlnThr: 1.528 ± 0.025	3.135GlnVal: 3.135 ± 0.037	0.613GlnTrp: 0.613 ± 0.015	0.677GlnTyr: 0.677 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	7.975ArgAla: 7.975 ± 0.069	0.637ArgCys: 0.637 ± 0.018	4.099ArgAsp: 4.099 ± 0.047	4.327ArgGlu: 4.327 ± 0.049	2.519ArgPhe: 2.519 ± 0.031	5.071ArgGly: 5.071 ± 0.051	1.809ArgHis: 1.809 ± 0.029	3.345ArgIle: 3.345 ± 0.042	1.906ArgLys: 1.906 ± 0.032	8.346ArgLeu: 8.346 ± 0.077	1.852ArgMet: 1.852 ± 0.028	1.604ArgAsn: 1.604 ± 0.027	4.166ArgPro: 4.166 ± 0.054	2.612ArgGln: 2.612 ± 0.036	6.559ArgArg: 6.559 ± 0.072	4.161ArgSer: 4.161 ± 0.046	4.51ArgThr: 4.51 ± 0.047	5.368ArgVal: 5.368 ± 0.06	1.427ArgTrp: 1.427 ± 0.025	1.874ArgTyr: 1.874 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.618SerAla: 6.618 ± 0.054	0.484SerCys: 0.484 ± 0.014	2.677SerAsp: 2.677 ± 0.036	2.545SerGlu: 2.545 ± 0.034	1.61SerPhe: 1.61 ± 0.029	5.864SerGly: 5.864 ± 0.06	0.961SerHis: 0.961 ± 0.02	1.974SerIle: 1.974 ± 0.032	1.268SerLys: 1.268 ± 0.029	5.086SerLeu: 5.086 ± 0.049	1.246SerMet: 1.246 ± 0.024	1.061SerAsn: 1.061 ± 0.025	3.067SerPro: 3.067 ± 0.038	1.475SerGln: 1.475 ± 0.028	3.597SerArg: 3.597 ± 0.039	3.035SerSer: 3.035 ± 0.046	3.727SerThr: 3.727 ± 0.049	4.467SerVal: 4.467 ± 0.048	1.072SerTrp: 1.072 ± 0.024	1.283SerTyr: 1.283 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	7.832ThrAla: 7.832 ± 0.068	0.51ThrCys: 0.51 ± 0.017	3.575ThrAsp: 3.575 ± 0.038	3.659ThrGlu: 3.659 ± 0.044	1.648ThrPhe: 1.648 ± 0.027	6.557ThrGly: 6.557 ± 0.054	1.139ThrHis: 1.139 ± 0.024	2.272ThrIle: 2.272 ± 0.033	1.465ThrLys: 1.465 ± 0.027	5.796ThrLeu: 5.796 ± 0.054	1.084ThrMet: 1.084 ± 0.021	1.226ThrAsn: 1.226 ± 0.026	3.68ThrPro: 3.68 ± 0.046	1.677ThrGln: 1.677 ± 0.03	3.732ThrArg: 3.732 ± 0.048	3.139ThrSer: 3.139 ± 0.041	3.689ThrThr: 3.689 ± 0.049	5.988ThrVal: 5.988 ± 0.058	0.906ThrTrp: 0.906 ± 0.022	1.264ThrTyr: 1.264 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	9.963ValAla: 9.963 ± 0.069	0.71ValCys: 0.71 ± 0.015	5.241ValAsp: 5.241 ± 0.052	4.248ValGlu: 4.248 ± 0.047	2.45ValPhe: 2.45 ± 0.038	6.339ValGly: 6.339 ± 0.061	1.921ValHis: 1.921 ± 0.027	3.687ValIle: 3.687 ± 0.045	1.768ValLys: 1.768 ± 0.031	10.035ValLeu: 10.035 ± 0.087	1.341ValMet: 1.341 ± 0.022	1.942ValAsn: 1.942 ± 0.029	4.79ValPro: 4.79 ± 0.05	2.455ValGln: 2.455 ± 0.032	6.187ValArg: 6.187 ± 0.057	4.486ValSer: 4.486 ± 0.047	5.367ValThr: 5.367 ± 0.059	7.139ValVal: 7.139 ± 0.065	1.086ValTrp: 1.086 ± 0.022	1.491ValTyr: 1.491 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.644TrpAla: 1.644 ± 0.027	0.164TrpCys: 0.164 ± 0.009	0.855TrpAsp: 0.855 ± 0.019	0.701TrpGlu: 0.701 ± 0.019	0.568TrpPhe: 0.568 ± 0.019	1.053TrpGly: 1.053 ± 0.024	0.388TrpHis: 0.388 ± 0.015	0.655TrpIle: 0.655 ± 0.017	0.383TrpLys: 0.383 ± 0.014	2.02TrpLeu: 2.02 ± 0.039	0.319TrpMet: 0.319 ± 0.012	0.429TrpAsn: 0.429 ± 0.015	0.888TrpPro: 0.888 ± 0.019	0.762TrpGln: 0.762 ± 0.02	1.321TrpArg: 1.321 ± 0.024	1.007TrpSer: 1.007 ± 0.024	1.057TrpThr: 1.057 ± 0.023	1.129TrpVal: 1.129 ± 0.021	0.387TrpTrp: 0.387 ± 0.013	0.336TrpTyr: 0.336 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.321TyrAla: 2.321 ± 0.032	0.204TyrCys: 0.204 ± 0.01	1.289TyrAsp: 1.289 ± 0.031	1.089TyrGlu: 1.089 ± 0.02	0.686TyrPhe: 0.686 ± 0.018	2.046TyrGly: 2.046 ± 0.034	0.429TyrHis: 0.429 ± 0.014	0.555TyrIle: 0.555 ± 0.016	0.442TyrLys: 0.442 ± 0.015	2.543TyrLeu: 2.543 ± 0.033	0.254TyrMet: 0.254 ± 0.011	0.491TyrAsn: 0.491 ± 0.017	1.214TyrPro: 1.214 ± 0.023	0.851TyrGln: 0.851 ± 0.019	1.975TyrArg: 1.975 ± 0.029	1.159TyrSer: 1.159 ± 0.023	1.221TyrThr: 1.221 ± 0.021	1.519TyrVal: 1.519 ± 0.026	0.424TyrTrp: 0.424 ± 0.015	0.504TyrTyr: 0.504 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7085 proteins (2221496 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---